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Protein

Acid phosphatase

Gene

phoA

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has both phosphomonoesterase and phosphodiesterase activity. Cleaves a broad range of phosphate esters.

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Enzyme regulationi

Inhibited by NaF, molybdate and vanadate.

Kineticsi

  1. KM=1.45 mM for p-nitrophenyl phophate1 Publication
  2. KM=2.3 mM for bis-(p-nitrophenyl) phophate1 Publication

    pH dependencei

    Optimum pH is 4-6. Active from pH 3 to 7.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei215Proton donorBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase
    Biological processCell wall biogenesis/degradation

    Enzyme and pathway databases

    BRENDAi3.1.3.2 508

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acid phosphatase (EC:3.1.3.2)
    Gene namesi
    Name:phoA
    ORF Names:AFUA_1G03570
    OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
    Taxonomic identifieri330879 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    Proteomesi
    • UP000002530 Componentsi: Chromosome 1, Unassembled WGS sequence

    Organism-specific databases

    EuPathDBiFungiDB:Afu1g03570

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 17Sequence analysisAdd BLAST17
    ChainiPRO_000024556018 – 419Acid phosphataseAdd BLAST402
    PropeptideiPRO_0000245561420 – 447Removed in mature formSequence analysisAdd BLAST28

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi119N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi150N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi177N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi186N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi208N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi217N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi234N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi240N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi315N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi332N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi382N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi405N-linked (GlcNAc...) asparagineSequence analysis1
    Lipidationi419GPI-like-anchor amidated serineSequence analysis1

    Post-translational modificationi

    The GPI-like anchor contains a phosphoceramide lipid group. The anchor position has not been determined.

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Expressioni

    Inductioni

    Repressed by phosphate.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ8X176
    SMRiQ8X176
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi412 – 419Poly-Ser8

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000212034
    InParanoidiQ8X176
    KOiK01078
    OrthoDBiEOG092C2GX5

    Family and domain databases

    InterProiView protein in InterPro
    IPR007312 Phosphoesterase
    PfamiView protein in Pfam
    PF04185 Phosphoesterase, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8X176-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKPSVATLLA TVSLVYAQTA TEKEPSLSAI ESAAASIQPY SPVSNVEGVA
    60 70 80 90 100
    FNRFFQVWLE NIDYEDAAAD ENMKWLASQG ILLTNFYAVT HPSEPNYCAA
    110 120 130 140 150
    VGGDTFGMDN DNFNQIPANV STVADLLDTK NIAWGEYQEH LPYPGFQGFN
    160 170 180 190 200
    YSNQETYVND YVRKHNPLVL YDSVTKNSTR LRQIKNFTSF EDDLANKKLP
    210 220 230 240 250
    QWAFITPNMT NDAHDTNITF GAKWERSWIA PLLNNSYFMN DTLILLTFDE
    260 270 280 290 300
    DGTYSKSNKI FSVLLGGAIP DELKGTQDDT FYTHYSVIAS VSANWGLPSL
    310 320 330 340 350
    GRWDCGANIL EIVANKTGYV NYDVDTTNLR LNETYPGPMS AGEYSKYSPV
    360 370 380 390 400
    WPNALTRGDC SAGHGILDIV KETYANTEPT YNYSSPFPYD TASNYNTKVT
    410 420 430 440
    ATKKNVTGTH RSSSSSSPSA SSNAAVSAVA PAAGVSGLLL GLALNLL
    Length:447
    Mass (Da):48,988
    Last modified:March 1, 2002 - v1
    Checksum:i4B2FB1F0ECEF0D72
    GO

    Sequence cautioni

    The sequence EAL88069 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti329L → C AA sequence (PubMed:11545413).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF462065 Genomic DNA Translation: AAL66381.1
    AAHF01000007 Genomic DNA Translation: EAL88069.1 Sequence problems.
    RefSeqiXP_750107.1, XM_745014.1

    Genome annotation databases

    GeneIDi3507810
    KEGGiafm:AFUA_1G03570

    Similar proteinsi

    Entry informationi

    Entry nameiPHOA_ASPFU
    AccessioniPrimary (citable) accession number: Q8X176
    Secondary accession number(s): Q4WK63
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: March 1, 2002
    Last modified: April 25, 2018
    This is version 76 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health