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Q8X168

- GANA_ASPNG

UniProt

Q8X168 - GANA_ASPNG

Protein

Arabinogalactan endo-beta-1,4-galactanase A

Gene

galA

Organism
Aspergillus niger
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Endogalactanase involved in the degradation of plant cell wall polysaccharides, and more particularly of hairy regions of pectin.By similarity

    Catalytic activityi

    The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.

    pH dependencei

    Optimum pH is between 4.0 and 4.5.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei152 – 1521Proton donorBy similarity
    Active sitei262 – 2621NucleophileBy similarity

    GO - Molecular functioni

    1. arabinogalactan endo-1,4-beta-galactosidase activity Source: UniProtKB
    2. glucosidase activity Source: InterPro

    GO - Biological processi

    1. pectin catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cell wall biogenesis/degradation, Polysaccharide degradation

    Protein family/group databases

    CAZyiGH53. Glycoside Hydrolase Family 53.
    mycoCLAPiGAN53A_ASPNG.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arabinogalactan endo-beta-1,4-galactanase A (EC:3.2.1.89)
    Alternative name(s):
    Endo-1,4-beta-galactanase A
    Short name:
    Galactanase A
    Gene namesi
    Name:galA
    OrganismiAspergillus niger
    Taxonomic identifieri5061 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Chaini17 – 350334Arabinogalactan endo-beta-1,4-galactanase APRO_0000394947Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Inductioni

    Specifically expressed on arabinose and galacturonic acid.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ8X168.
    SMRiQ8X168. Positions 17-350.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 53 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3867.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR011683. Glyco_hydro_53.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF07745. Glyco_hydro_53. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8X168-1 [UniParc]FASTAAdd to Basket

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    MIYPLLLSAL PLLSSAALTY RGADISSLLI EEDAGISYKN LNGETQALED    50
    ILVNNGVNSI RQRVWVDPSD GSYDLDYNLK LAKRVQAAGM SIYLDLHLSD 100
    TWADPSDQTT PTGWSTTDID TLTWQLYNYT LDVCNTFAEN DIDIEIVSIG 150
    NEISSGLLWP LGKTSNYDNI AKLLHSGAWG VKDSNQATTP KIMIHLDNGW 200
    DWEEQEYFYK TVLATGSLLS TDFDLMGVSY YPFYNSEATL SALQTSLTNM 250
    QSNYDKSVVV VETNWPVSCP DPEYSFPSDL SSIPFSAAGQ EEFLEKLAEV 300
    VEGVTDGLGI YYWEPAWVDN AALGSSCADN LMVDIDTDEV LESVTVFEDL 350
    Length:350
    Mass (Da):38,734
    Last modified:March 1, 2002 - v1
    Checksum:i3353286DECB85DD6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ305303 Genomic DNA. Translation: CAC83735.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ305303 Genomic DNA. Translation: CAC83735.1 .

    3D structure databases

    ProteinModelPortali Q8X168.
    SMRi Q8X168. Positions 17-350.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH53. Glycoside Hydrolase Family 53.
    mycoCLAPi GAN53A_ASPNG.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG3867.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR011683. Glyco_hydro_53.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF07745. Glyco_hydro_53. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Endogalactanase A from Aspergillus niger is specifically induced on L-arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions."
      de Vries R.P., Paenicova L., Hinz S., Kester H.C.M., Benen J.A.E., Beldman G.A., Visser J.
      Eur. J. Biochem. 269:4985-4993(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, SUBCELLULAR LOCATION, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.

    Entry informationi

    Entry nameiGANA_ASPNG
    AccessioniPrimary (citable) accession number: Q8X168
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 15, 2010
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3