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Q8X168

- GANA_ASPNG

UniProt

Q8X168 - GANA_ASPNG

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Protein
Arabinogalactan endo-beta-1,4-galactanase A
Gene
galA
Organism
Aspergillus niger
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Endogalactanase involved in the degradation of plant cell wall polysaccharides, and more particularly of hairy regions of pectin By similarity.1 Publication

Catalytic activityi

The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.

pH dependencei

Optimum pH is between 4.0 and 4.5.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei152 – 1521Proton donor By similarity
Active sitei262 – 2621Nucleophile By similarity

GO - Molecular functioni

  1. arabinogalactan endo-1,4-beta-galactosidase activity Source: UniProtKB
  2. glucosidase activity Source: InterPro

GO - Biological processi

  1. pectin catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH53. Glycoside Hydrolase Family 53.
mycoCLAPiGAN53A_ASPNG.

Names & Taxonomyi

Protein namesi
Recommended name:
Arabinogalactan endo-beta-1,4-galactanase A (EC:3.2.1.89)
Alternative name(s):
Endo-1,4-beta-galactanase A
Short name:
Galactanase A
Gene namesi
Name:galA
OrganismiAspergillus niger
Taxonomic identifieri5061 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616 Reviewed prediction
Add
BLAST
Chaini17 – 350334Arabinogalactan endo-beta-1,4-galactanase A
PRO_0000394947Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi128 – 1281N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Expressioni

Inductioni

Specifically expressed on arabinose and galacturonic acid.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8X168.
SMRiQ8X168. Positions 17-350.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3867.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR011683. Glyco_hydro_53.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF07745. Glyco_hydro_53. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8X168-1 [UniParc]FASTAAdd to Basket

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MIYPLLLSAL PLLSSAALTY RGADISSLLI EEDAGISYKN LNGETQALED    50
ILVNNGVNSI RQRVWVDPSD GSYDLDYNLK LAKRVQAAGM SIYLDLHLSD 100
TWADPSDQTT PTGWSTTDID TLTWQLYNYT LDVCNTFAEN DIDIEIVSIG 150
NEISSGLLWP LGKTSNYDNI AKLLHSGAWG VKDSNQATTP KIMIHLDNGW 200
DWEEQEYFYK TVLATGSLLS TDFDLMGVSY YPFYNSEATL SALQTSLTNM 250
QSNYDKSVVV VETNWPVSCP DPEYSFPSDL SSIPFSAAGQ EEFLEKLAEV 300
VEGVTDGLGI YYWEPAWVDN AALGSSCADN LMVDIDTDEV LESVTVFEDL 350
Length:350
Mass (Da):38,734
Last modified:March 1, 2002 - v1
Checksum:i3353286DECB85DD6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ305303 Genomic DNA. Translation: CAC83735.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ305303 Genomic DNA. Translation: CAC83735.1 .

3D structure databases

ProteinModelPortali Q8X168.
SMRi Q8X168. Positions 17-350.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH53. Glycoside Hydrolase Family 53.
mycoCLAPi GAN53A_ASPNG.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG3867.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR011683. Glyco_hydro_53.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF07745. Glyco_hydro_53. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Endogalactanase A from Aspergillus niger is specifically induced on L-arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions."
    de Vries R.P., Paenicova L., Hinz S., Kester H.C.M., Benen J.A.E., Beldman G.A., Visser J.
    Eur. J. Biochem. 269:4985-4993(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, SUBCELLULAR LOCATION, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.

Entry informationi

Entry nameiGANA_ASPNG
AccessioniPrimary (citable) accession number: Q8X168
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: March 1, 2002
Last modified: June 11, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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