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Protein

D-xylulose kinase A

Gene

xkiA

Organism
Aspergillus niger
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Highly specific D-xylulose kinase which participates in the catabolism of xylose. Xylose is a major component of hemicelluloses such as xylan. Most fungi utilize D-xylose via three enzymatic reactions, xylose reductase (XR), xylitol dehydrogenase (XDH), and xylulokinase, to form xylulose 5-phosphate, which enters pentose phosphate pathway.1 Publication

Catalytic activityi

ATP + D-xylulose = ADP + D-xylulose 5-phosphate.

Kineticsi

  1. KM=0.76 mM for D-xylulose1 Publication
  2. KM=0.061 mM for ATP1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei95 – 951SubstrateBy similarity
    Binding sitei166 – 1661SubstrateBy similarity
    Binding sitei282 – 2821SubstrateBy similarity
    Binding sitei283 – 2831SubstrateBy similarity
    Binding sitei364 – 3641ATPBy similarity
    Binding sitei473 – 4731ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi469 – 4702ATPBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Xylose metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13191.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-xylulose kinase A (EC:2.7.1.17)
    Short name:
    Xylulokinase A
    Gene namesi
    Name:xkiA
    OrganismiAspergillus niger
    Taxonomic identifieri5061 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 570570D-xylulose kinase APRO_0000393521Add
    BLAST

    Expressioni

    Inductioni

    By D-xylose, L-arabinose or L-arabitol.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi5061.CADANGAP00005475.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8X167.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FGGY kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG1070.

    Family and domain databases

    InterProiIPR018485. Carb_kinase_FGGY_C.
    IPR018484. Carb_kinase_FGGY_N.
    [Graphical view]
    PfamiPF02782. FGGY_C. 1 hit.
    PF00370. FGGY_N. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8X167-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MQGPLYIGFD LSTQQLKGLV VNSDLKVVYV SKFDFDADSR GFPIKKGVLT
    60 70 80 90 100
    NEAEHEVFAP VALWLQALDG VLEGLRKQGM DFSQIKGISG AGQQHGSVYW
    110 120 130 140 150
    GENAEKLLKE LDASKTLEEQ LDGAFSHPFS PNWQDSSTQK ECDEFDAALG
    160 170 180 190 200
    GQSELAFATG SKAHHRFTGP QIMRFQRKYP DVYKKTSRIS LVSSFIASLF
    210 220 230 240 250
    LGHIAPMDIS DVCGMNLWNI KKGAYDEKLL QLCAGSSGVD DLKRKLGDVP
    260 270 280 290 300
    EDGGIHLGPI DRYYVERYGF SPDCTIIPAT GDNPATILAL PLRASDAMVS
    310 320 330 340 350
    LGTSTTFLMS TPSYKPDPAT HFFNHPTTAG LYMFMLCYKN GGLARELVRD
    360 370 380 390 400
    AVNEKLGEKP STSWANFDKV TLETPPMGQK ADSDPMKLGL FFPRPEIVPN
    410 420 430 440 450
    LRSGQWRFDY NPKDGSLQPS NGGWDEPFDE ARAIVESQML SLRLRSRGLT
    460 470 480 490 500
    QSPGEGIPAQ PRRVYLVGGG SKNKAIAKVA GEILGGSEGV YKLEIGDNAC
    510 520 530 540 550
    ALGAAYKAVW AMERAEGQTF EDLIGKRWHE EEFIEKIADG YQPGVFERYG
    560 570
    QAVEGFEKME LEVLRQEGKH
    Length:570
    Mass (Da):62,809
    Last modified:March 1, 2002 - v1
    Checksum:iDB28E6CC2728EE86
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ305311 Genomic DNA. Translation: CAC83746.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ305311 Genomic DNA. Translation: CAC83746.1.

    3D structure databases

    ProteinModelPortaliQ8X167.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi5061.CADANGAP00005475.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Phylogenomic databases

    eggNOGiCOG1070.

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13191.

    Family and domain databases

    InterProiIPR018485. Carb_kinase_FGGY_C.
    IPR018484. Carb_kinase_FGGY_N.
    [Graphical view]
    PfamiPF02782. FGGY_C. 1 hit.
    PF00370. FGGY_N. 2 hits.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    1. "The Aspergillus niger D-xylulose kinase gene is co-expressed with genes encoding arabinan degrading enzymes, and is essential for growth on D-xylose and L-arabinose."
      vanKuyk P.A., de Groot M.J., Ruijter G.J., de Vries R.P., Visser J.
      Eur. J. Biochem. 268:5414-5423(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.

    Entry informationi

    Entry nameiXKS1_ASPNG
    AccessioniPrimary (citable) accession number: Q8X167
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 20, 2010
    Last sequence update: March 1, 2002
    Last modified: June 24, 2015
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.