Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8X167 (XKS1_ASPNG) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-xylulose kinase A

Short name=Xylulokinase A
EC=2.7.1.17
Gene names
Name:xkiA
OrganismAspergillus niger
Taxonomic identifier5061 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length570 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Highly specific D-xylulose kinase which participates in the catabolism of xylose. Xylose is a major component of hemicelluloses such as xylan. Most fungi utilize D-xylose via three enzymatic reactions, xylose reductase (XR), xylitol dehydrogenase (XDH), and xylulokinase, to form xylulose 5-phosphate, which enters pentose phosphate pathway. Ref.1

Catalytic activity

ATP + D-xylulose = ADP + D-xylulose 5-phosphate.

Subcellular location

Cytoplasm By similarity.

Induction

By D-xylose, L-arabinose or L-arabitol. Ref.1

Sequence similarities

Belongs to the FGGY kinase family.

Biophysicochemical properties

Kinetic parameters:

KM=0.76 mM for D-xylulose Ref.1

KM=0.061 mM for ATP

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Xylose metabolism
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
Gene Ontology (GO)
   Biological_processD-xylose metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

xylulokinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 570570D-xylulose kinase A
PRO_0000393521

Regions

Nucleotide binding469 – 4702ATP By similarity

Sites

Binding site951Substrate By similarity
Binding site1661Substrate By similarity
Binding site2821Substrate By similarity
Binding site2831Substrate By similarity
Binding site3641ATP By similarity
Binding site4731ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8X167 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: DB28E6CC2728EE86

FASTA57062,809
        10         20         30         40         50         60 
MQGPLYIGFD LSTQQLKGLV VNSDLKVVYV SKFDFDADSR GFPIKKGVLT NEAEHEVFAP 

        70         80         90        100        110        120 
VALWLQALDG VLEGLRKQGM DFSQIKGISG AGQQHGSVYW GENAEKLLKE LDASKTLEEQ 

       130        140        150        160        170        180 
LDGAFSHPFS PNWQDSSTQK ECDEFDAALG GQSELAFATG SKAHHRFTGP QIMRFQRKYP 

       190        200        210        220        230        240 
DVYKKTSRIS LVSSFIASLF LGHIAPMDIS DVCGMNLWNI KKGAYDEKLL QLCAGSSGVD 

       250        260        270        280        290        300 
DLKRKLGDVP EDGGIHLGPI DRYYVERYGF SPDCTIIPAT GDNPATILAL PLRASDAMVS 

       310        320        330        340        350        360 
LGTSTTFLMS TPSYKPDPAT HFFNHPTTAG LYMFMLCYKN GGLARELVRD AVNEKLGEKP 

       370        380        390        400        410        420 
STSWANFDKV TLETPPMGQK ADSDPMKLGL FFPRPEIVPN LRSGQWRFDY NPKDGSLQPS 

       430        440        450        460        470        480 
NGGWDEPFDE ARAIVESQML SLRLRSRGLT QSPGEGIPAQ PRRVYLVGGG SKNKAIAKVA 

       490        500        510        520        530        540 
GEILGGSEGV YKLEIGDNAC ALGAAYKAVW AMERAEGQTF EDLIGKRWHE EEFIEKIADG 

       550        560        570 
YQPGVFERYG QAVEGFEKME LEVLRQEGKH 

« Hide

References

[1]"The Aspergillus niger D-xylulose kinase gene is co-expressed with genes encoding arabinan degrading enzymes, and is essential for growth on D-xylose and L-arabinose."
vanKuyk P.A., de Groot M.J., Ruijter G.J., de Vries R.P., Visser J.
Eur. J. Biochem. 268:5414-5423(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ305311 Genomic DNA. Translation: CAC83746.1.

3D structure databases

ProteinModelPortalQ8X167.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG1070.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13191.

Family and domain databases

InterProIPR018485. Carb_kinase_FGGY_C.
IPR018484. Carb_kinase_FGGY_N.
[Graphical view]
PfamPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXKS1_ASPNG
AccessionPrimary (citable) accession number: Q8X167
Entry history
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: March 1, 2002
Last modified: April 16, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families