Q8X167 (XKS1_ASPNG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-xylulose kinase A
Short name=Xylulokinase A
EC=2.7.1.17

Gene names

Name:

xkiA

Organism

Aspergillus niger

Taxonomic identifier

5061 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Highly specific D-xylulose kinase which participates in the catabolism of xylose. Xylose is a major component of hemicelluloses such as xylan. Most fungi utilize D-xylose via three enzymatic reactions, xylose reductase (XR), xylitol dehydrogenase (XDH), and xylulokinase, to form xylulose 5-phosphate, which enters pentose phosphate pathway. Ref.1
Catalytic activity	ATP + D-xylulose = ADP + D-xylulose 5-phosphate.
Subcellular location	Cytoplasm By similarity.
Induction	By D-xylose, L-arabinose or L-arabitol. Ref.1
Sequence similarities	Belongs to the FGGY kinase family.
Biophysicochemical properties	Kinetic parameters: KM=0.76 mM for D-xylulose Ref.1 KM=0.061 mM for ATP

Ontologies

Keywords
Biological process	Carbohydrate metabolism Xylose metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	D-xylose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW xylulokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 570	570	D-xylulose kinase A		PRO_0000393521

Sequences

	Sequence		Length	Mass (Da)
	Q8X167 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: DB28E6CC2728EE86	FASTA	570	62,809
	10 20 30 40 50 60 MQGPLYIGFD LSTQQLKGLV VNSDLKVVYV SKFDFDADSR GFPIKKGVLT NEAEHEVFAP 70 80 90 100 110 120 VALWLQALDG VLEGLRKQGM DFSQIKGISG AGQQHGSVYW GENAEKLLKE LDASKTLEEQ 130 140 150 160 170 180 LDGAFSHPFS PNWQDSSTQK ECDEFDAALG GQSELAFATG SKAHHRFTGP QIMRFQRKYP 190 200 210 220 230 240 DVYKKTSRIS LVSSFIASLF LGHIAPMDIS DVCGMNLWNI KKGAYDEKLL QLCAGSSGVD 250 260 270 280 290 300 DLKRKLGDVP EDGGIHLGPI DRYYVERYGF SPDCTIIPAT GDNPATILAL PLRASDAMVS 310 320 330 340 350 360 LGTSTTFLMS TPSYKPDPAT HFFNHPTTAG LYMFMLCYKN GGLARELVRD AVNEKLGEKP 370 380 390 400 410 420 STSWANFDKV TLETPPMGQK ADSDPMKLGL FFPRPEIVPN LRSGQWRFDY NPKDGSLQPS 430 440 450 460 470 480 NGGWDEPFDE ARAIVESQML SLRLRSRGLT QSPGEGIPAQ PRRVYLVGGG SKNKAIAKVA 490 500 510 520 530 540 GEILGGSEGV YKLEIGDNAC ALGAAYKAVW AMERAEGQTF EDLIGKRWHE EEFIEKIADG 550 560 570 YQPGVFERYG QAVEGFEKME LEVLRQEGKH

References

[1]

"The Aspergillus niger D-xylulose kinase gene is co-expressed with genes encoding arabinan degrading enzymes, and is essential for growth on D-xylose and L-arabinose." vanKuyk P.A., de Groot M.J., Ruijter G.J., de Vries R.P., Visser J. Eur. J. Biochem. 268:5414-5423(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ305311 Genomic DNA. Translation: CAC83746.1.
3D structure databases
ProteinModelPortal	Q8X167.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG1070.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13191.
Family and domain databases
InterPro	IPR018485. Carb_kinase_FGGY_C. IPR018484. Carb_kinase_FGGY_N. [Graphical view]
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 2 hits. [Graphical view]
PROSITE	PS00933. FGGY_KINASES_1. False negative. PS00445. FGGY_KINASES_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XKS1_ASPNG

Accession

Primary (citable) accession number: Q8X167

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 20, 2010
Last sequence update:	March 1, 2002
Last modified:	April 3, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program