Q8X0Z1 (LCPS_GIBFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional lycopene cyclase/phytoene synthase

Including the following 2 domains:

1. Lycopene beta-cyclase
   EC=5.5.1.19
   Alternative name(s):
   Carotene cyclase
   Lycopene cyclase
2. Phytoene synthase
   EC=2.5.1.32

Gene names

Name:

carRA

Organism

Gibberella fujikuroi (Bakanae and foot rot disease fungus) (Fusarium moniliforme)

Taxonomic identifier

5127 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Gibberella › Fusarium fujikuroi complex

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene and from lycopene to beta-carotene via the intermediate gamma-carotene. Gamma-carotene is further processed to the acidic carotenoid neurosporaxanthin. Ref.1
Catalytic activity	Carotenoid psi-end group = carotenoid beta-end group. 2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.
Pathway	Carotenoid biosynthesis; beta-carotene biosynthesis. Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Induction	Induced by light. Ref.1
Sequence similarities	In the N-terminal section; belongs to the lycopene beta-cyclase family. In the C-terminal section; belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Isomerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	geranylgeranyl-diphosphate geranylgeranyltransferase activity Inferred from mutant phenotype Ref.1. Source: UniProtKB intramolecular lyase activity Inferred from electronic annotation. Source: InterPro lycopene beta cyclase activity Inferred from mutant phenotype Ref.1. Source: UniProtKB phytoene synthase activity Inferred from mutant phenotype Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 612	612	Bifunctional lycopene cyclase/phytoene synthase		PRO_0000409236
Regions
	Transmembrane	3 – 23	21	Helical; Potential
	Transmembrane	31 – 51	21	Helical; Potential
	Transmembrane	112 – 130	19	Helical; Potential
	Transmembrane	148 – 168	21	Helical; Potential
	Transmembrane	171 – 191	21	Helical; Potential
	Transmembrane	203 – 223	21	Helical; Potential
	Transmembrane	246 – 266	21	Helical; Potential
	Region	1 – 268	268	Lycopene beta-cyclase
	Region	275 – 612	338	Phytoene synthase

Sequences

	Sequence		Length	Mass (Da)
	Q8X0Z1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 14F7D731B993AB65	FASTA	612	69,458
	10 20 30 40 50 60 MGWEYAQVHL KYTIPFGVVL AAVYRPLMSR LDVFKLVFLI TVSFFWVVKG LEANSCRLLL 70 80 90 100 110 120 FLPCMLILRG VLLRLTNADS PWDSYLIKNR IWTYPPGVVV GLTAWDIPAE ELFFFVIQTF 130 140 150 160 170 180 NTSLLYMILS KPTFHPIYLS KKTGWGKIAG QILFASAIIF GLVSVSSGGE GMYMGLILIW 190 200 210 220 230 240 ACPFLLFLWS ISYQFIVNLP WTNTALPIAL PTLYLWVVDT FALRRGTWSI TSGTKYGVVL 250 260 270 280 290 300 WDGLEIEEAV FFLLTNTLIV FGLIACDNNL AILDTFPEHF PRTKGVPSLL TIIRTLILPK 310 320 330 340 350 360 EKYDEERIQG LVSAVALLRK KSRSFYLASG TFEGRLRIDL IRLYAFCRAA DDLVDEAPSV 370 380 390 400 410 420 DDSRASIEKL RKFLDLAYEE NQEEPSQRLR EYVTSSIPEM FHMALLQLPT YYLPKQPLDD 430 440 450 460 470 480 LLKGFDTDLL FDRKSGAFPI ETTEDLDIYG SRVAGTVAEL CNHLILYHTP EAVPEDIQRE 490 500 510 520 530 540 VVASGQEMGI ALQYVNIARD IKTDAEIDRV YLPLSWLKEA QLTPEDVIQQ PHGPTIEALR 550 560 570 580 590 600 HKLLDRAFEK YNMAKGAIDK LPSEGKGPIR VAVESYMEIG RVLREKGPTM KKGRATVPKM 610 RRIRVAWSAL NK

References

[1]

"A carotenoid biosynthesis gene cluster in Fusarium fujikuroi: the genes carB and carRA." Linnemannstons P., Prado M.M., Fernandez-Martin R., Tudzynski B., Avalos J. Mol. Genet. Genomics 267:593-602(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ426417 Genomic DNA. Translation: CAD19988.1.
3D structure databases
ProteinModelPortal	Q8X0Z1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00799; UER00773. UPA00802.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR017825. Lycopene_cyclase_dom. IPR002060. Squ/phyt_synthse. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
TIGRFAMs	TIGR03462. CarR_dom_SF. 2 hits.
PROSITE	PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LCPS_GIBFU

Accession

Primary (citable) accession number: Q8X0Z1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	March 1, 2002
Last modified:	April 3, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program