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Q8X0Z1 (LCPS_GIBFU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional lycopene cyclase/phytoene synthase

Including the following 2 domains:

  1. Lycopene beta-cyclase
    EC=5.5.1.19
    Alternative name(s):
    Carotene cyclase
    Lycopene cyclase
  2. Phytoene synthase
    EC=2.5.1.32
Gene names
Name:carRA
OrganismGibberella fujikuroi (Bakanae and foot rot disease fungus) (Fusarium fujikuroi)
Taxonomic identifier5127 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium fujikuroi species complex

Protein attributes

Sequence length612 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene and from lycopene to beta-carotene via the intermediate gamma-carotene. Gamma-carotene is further processed to the acidic carotenoid neurosporaxanthin. Ref.1

Catalytic activity

Carotenoid psi-end group = carotenoid beta-end group.

2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

Pathway

Carotenoid biosynthesis; beta-carotene biosynthesis.

Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Induction

Induced by light. Ref.1

Sequence similarities

In the N-terminal section; belongs to the lycopene beta-cyclase family.

In the C-terminal section; belongs to the phytoene/squalene synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 612612Bifunctional lycopene cyclase/phytoene synthase
PRO_0000409236

Regions

Transmembrane3 – 2321Helical; Potential
Transmembrane31 – 5121Helical; Potential
Transmembrane112 – 13019Helical; Potential
Transmembrane148 – 16821Helical; Potential
Transmembrane171 – 19121Helical; Potential
Transmembrane203 – 22321Helical; Potential
Transmembrane246 – 26621Helical; Potential
Region1 – 268268Lycopene beta-cyclase
Region275 – 612338Phytoene synthase

Sequences

Sequence LengthMass (Da)Tools
Q8X0Z1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 14F7D731B993AB65

FASTA61269,458
        10         20         30         40         50         60 
MGWEYAQVHL KYTIPFGVVL AAVYRPLMSR LDVFKLVFLI TVSFFWVVKG LEANSCRLLL 

        70         80         90        100        110        120 
FLPCMLILRG VLLRLTNADS PWDSYLIKNR IWTYPPGVVV GLTAWDIPAE ELFFFVIQTF 

       130        140        150        160        170        180 
NTSLLYMILS KPTFHPIYLS KKTGWGKIAG QILFASAIIF GLVSVSSGGE GMYMGLILIW 

       190        200        210        220        230        240 
ACPFLLFLWS ISYQFIVNLP WTNTALPIAL PTLYLWVVDT FALRRGTWSI TSGTKYGVVL 

       250        260        270        280        290        300 
WDGLEIEEAV FFLLTNTLIV FGLIACDNNL AILDTFPEHF PRTKGVPSLL TIIRTLILPK 

       310        320        330        340        350        360 
EKYDEERIQG LVSAVALLRK KSRSFYLASG TFEGRLRIDL IRLYAFCRAA DDLVDEAPSV 

       370        380        390        400        410        420 
DDSRASIEKL RKFLDLAYEE NQEEPSQRLR EYVTSSIPEM FHMALLQLPT YYLPKQPLDD 

       430        440        450        460        470        480 
LLKGFDTDLL FDRKSGAFPI ETTEDLDIYG SRVAGTVAEL CNHLILYHTP EAVPEDIQRE 

       490        500        510        520        530        540 
VVASGQEMGI ALQYVNIARD IKTDAEIDRV YLPLSWLKEA QLTPEDVIQQ PHGPTIEALR 

       550        560        570        580        590        600 
HKLLDRAFEK YNMAKGAIDK LPSEGKGPIR VAVESYMEIG RVLREKGPTM KKGRATVPKM 

       610 
RRIRVAWSAL NK 

« Hide

References

[1]"A carotenoid biosynthesis gene cluster in Fusarium fujikuroi: the genes carB and carRA."
Linnemannstons P., Prado M.M., Fernandez-Martin R., Tudzynski B., Avalos J.
Mol. Genet. Genomics 267:593-602(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ426417 Genomic DNA. Translation: CAD19988.1.

3D structure databases

ProteinModelPortalQ8X0Z1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00799; UER00773.
UPA00802.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
InterProIPR017825. Lycopene_cyclase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMSSF48576. SSF48576. 1 hit.
TIGRFAMsTIGR03462. CarR_dom_SF. 2 hits.
PROSITEPS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLCPS_GIBFU
AccessionPrimary (citable) accession number: Q8X0Z1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: March 1, 2002
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways