Reviewed, UniProtKB/Swiss-Prot Q8X097 (ACL1_NEUCR)

Names and origin

Protein names

Recommended name:
    Probable ATP-citrate synthase subunit 1
    EC=2.3.3.8
Alternative name(s):
    ATP-citrate (pro-S-)-lyase 1
    Citrate cleavage enzyme subunit 1

Gene names

ORF Names:

B14D6.310, NCU06785

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	670 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of cytosolic acetyl-CoA, which is mainly used for the biosynthesis of fatty acids and sterols By similarity.
Catalytic activity	ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA.
Subunit structure	Composed of two subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the succinate/malate CoA ligase alpha subunit family.

Ontologies

Keywords
Biological process	Lipid synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP citrate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW succinate-CoA ligase (ADP-forming) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 670	670	Probable ATP-citrate synthase subunit 1		PRO_0000102785
Regions
	Nucleotide binding	257 – 277	21	ATP By similarity
	Nucleotide binding	308 – 334	27	ATP By similarity
	Region	335 – 345	11	CoA-binding Potential
Sites
	Active site	316	1	Tele-phosphohistidine intermediate By similarity
	Metal binding	274	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8X097-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 3AC73BA19AA5EDFE	FASTA	670	72,627
	10 20 30 40 50 60 MPSATTASTN GANGASASPA PGNLSANDNI RRFAAPSRPL SPLPAHALFN DKTRCFVYGL 70 80 90 100 110 120 QPRAVQGMLD FDFICKRSTP SVAGIIYTFG GQFVSKMYWG TSETLLPVYQ EVPKAIAKHP 130 140 150 160 170 180 DVDVVVNFAS SRSVYSSTME LMEYPQIKTI AIIAEGVPER RAREIAYVAK KKGITIIGPA 190 200 210 220 230 240 TVGGIKPGCF KIGNTGGMMD NIVASKLYRK GSVGYVSKSG GMSNELNNII SQTTDGVYEG 250 260 270 280 290 300 VAIGGDRYPG TTFIDHLLRY QADPDCKILV LLGEVGGVEE YKVIDAVKQG IITKPIVAWA 310 320 330 340 350 360 IGTCASMFKT EVQFGHAGAF ANSQLETAAT KNKSMREAGF YVPDTFEDMP ALLKQVYDKL 370 380 390 400 410 420 VADGTIVPAP EPVVPKIPID YSWAQELGLI RKPAAFISTI SDDRGQELLY AGMPISDVFK 430 440 450 460 470 480 EEIGIGGVMS LLWFRRRLPD YAAKFLEMVL MLTADHGPAV SGAMNTIITT RAGKDLISSL 490 500 510 520 530 540 VAGLLTIGSR FGGALDGAAE EFTKAFDKGL SPREFVDTMR KQNKLIPGIG HRVKSRNNPD 550 560 570 580 590 600 LRVELVKEYV KAKFPSTKLL DYALAVESVT TSKKDNLILN VDGCIAVCFV DLLRNCGAFS 610 620 630 640 650 660 TEEAEDYLSM GVLNGLFVLG RSIGLIAHYL DQKRLRTGLY RHPWDDITYL LPSLQQPGPP 670 GTEGRVEVQI

References

[1]

"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

[2]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL356173 Genomic DNA. Translation: CAB91740.2. AABX02000033 Genomic DNA. Translation: EAA34390.1.
RefSeq	XP_963626.1.
3D structure databases
HSSP	HSSP built from PDB template 1OI7 based on UniProtKB P09143.
ModBase	Search...
Genome annotation databases
GeneID	3879766.
KEGG	ncr:NCU06785.
NMPDR	fig|5141.1.peg.5004.
Enzyme and pathway databases
BioCyc	NCRA-XX3-01:NCRA-XX3-01-001644-MON.
BRENDA	2.3.3.8. 266.
Family and domain databases
InterPro	IPR017440. Cit_synth/succinyl-CoA_lig_AS. IPR016142. Citrate_synth-like_lrg_a-sub. IPR003781. CoA_bd. IPR005810. CoA_lig_alpha. IPR005811. CoA_ligase. IPR016040. NAD(P)-bd_dom. IPR017866. Succ-CoA_synthase_bsu_CS. IPR016102. Succinyl-CoA_synth-like. [Graphical view]
Gene3D	G3DSA:1.10.580.10. Citrate_synthase_lrg_a-sub. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:3.40.50.261. Succinyl-CoA_synth-like. 1 hit.
Pfam	PF02629. CoA_binding. 1 hit. PF00549. Ligase_CoA. 1 hit. [Graphical view]
PRINTS	PR01798. SCOASYNTHASE.
PROSITE	PS01216. SUCCINYL_COA_LIG_1. 1 hit. PS00399. SUCCINYL_COA_LIG_2. 1 hit. PS01217. SUCCINYL_COA_LIG_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACL1_NEUCR

Accession

Primary (citable) accession number: Q8X097
Secondary accession number(s): Q7SCH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	March 1, 2002
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)