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Protein

GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase

Gene

alg-11

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for N-linked oligosaccharide assembly. Has a role in the last step of the synthesis of the Man5GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum (By similarity).By similarity

Catalytic activityi

2 GDP-alpha-D-mannose + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = 2 GDP + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.

Pathwayi

GO - Molecular functioni

  1. GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. oligosaccharide-lipid intermediate biosynthetic process Source: EnsemblFungi
  2. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase (EC:2.4.1.131)
Alternative name(s):
Alpha-1,2-mannosyltransferase alg-11
Asparagine-linked glycosylation protein 11
Glycolipid 2-alpha-mannosyltransferase
Gene namesi
Name:alg-11
ORF Names:B14D6.360, NCU06779
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805 Componenti: Chromosome 6, Linkage Group II

Organism-specific databases

EuPathDBiFungiDB:NCU06779.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini29 – 556528LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferasePRO_0000080277Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi81 – 811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi314 – 3141N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi5141.NCU06779.1.

Structurei

3D structure databases

ProteinModelPortaliQ8X092.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0438.
HOGENOMiHOG000209670.
InParanoidiQ8X092.
KOiK03844.
OMAiWEALTAF.
OrthoDBiEOG7673KQ.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8X092-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANGLFTYVA ISLFTIGPLL ALFIPFVWRL VGSSLGWYLR RKTDGRRCHI
60 70 80 90 100
LEVVEADERK YRDSKSAKGR KAEKEDRDTF NNTEATGTSD GKVYDKDWDG
110 120 130 140 150
IVGFFHPFCN AGGGGERVLW AAIRATQKRW PKAKCVVYTG DHDVSKEAIL
160 170 180 190 200
SRVEQRFNIH LHPPTVNFLY LSTRRWVLAS TWPYFTLAGQ SFGSLIMAWD
210 220 230 240 250
AFSLLVPDIF VDTMGYAFAL GFSRFLFRDV PTAAYVHYPT ISTDMLESLD
260 270 280 290 300
PASAVGSQGV NAGKGTGAKG RAKKIYWQLF ARLYSLMGAS VDVVMTNSTW
310 320 330 340 350
TQAHIEKLWG PVRNLTGAVP GIKSKVNPIA VVYPPVAVEE LEQEVEVSPE
360 370 380 390 400
SEKRRENVLL YIAQFRPEKN HQLIVQAFAE FLKSGSEAAR DAKLVLVGSV
410 420 430 440 450
RDDYDSKRVY KLRLLVNELH IKDRVEFHLD ASWPDILEWL RRASVGVNGM
460 470 480 490 500
WNEHFGIGVV EYQAAGLISV VHDSGGPKLD IVVEVDGEPT GFHATTSKEF
510 520 530 540 550
AEGFEKALSL PNPYAVRLRA RKSAKRFTEE EFARRWIEQL EKCVAIKAVE

KPKSRQ
Length:556
Mass (Da):62,533
Last modified:March 1, 2002 - v1
Checksum:i3D59DA2B140B287A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL356173 Genomic DNA. Translation: CAB91745.2.
CM002237 Genomic DNA. Translation: EAA34384.1.
RefSeqiXP_963620.1. XM_958527.3.
UniGeneiNcr.23849.

Genome annotation databases

EnsemblFungiiEFNCRT00000006888; EFNCRP00000006879; EFNCRG00000006876.
GeneIDi3879830.
KEGGincr:NCU06779.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL356173 Genomic DNA. Translation: CAB91745.2.
CM002237 Genomic DNA. Translation: EAA34384.1.
RefSeqiXP_963620.1. XM_958527.3.
UniGeneiNcr.23849.

3D structure databases

ProteinModelPortaliQ8X092.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5141.NCU06779.1.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFNCRT00000006888; EFNCRP00000006879; EFNCRG00000006876.
GeneIDi3879830.
KEGGincr:NCU06779.

Organism-specific databases

EuPathDBiFungiDB:NCU06779.

Phylogenomic databases

eggNOGiCOG0438.
HOGENOMiHOG000209670.
InParanoidiQ8X092.
KOiK03844.
OMAiWEALTAF.
OrthoDBiEOG7673KQ.

Enzyme and pathway databases

UniPathwayiUPA00378.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
    Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
    Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiALG11_NEUCR
AccessioniPrimary (citable) accession number: Q8X092
Secondary accession number(s): Q1K8Z1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: March 1, 2002
Last modified: April 29, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.