Q8WZK3 (IBP1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dual specificity phosphatase ibp1
EC=3.1.3.48

Alternative name(s):
Cdc25-like phosphatase ibp1
Itsy bitsy phosphatase 1

Gene names

Name:	ibp1
ORF Names:	SPBC839.07

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in DNA replication checkpoint via regulation of hsk1 or may act downstream of hsk1 in an S phase regulatory pathway. Ref.1
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subcellular location	Cytoplasm. Nucleus Ref.1 Ref.3.
Sequence similarities	Belongs to the MPI phosphatase family. Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Nucleus
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent DNA replication Inferred from genetic interaction Ref.1. Source: PomBase cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC regulation of DNA-dependent DNA replication Inferred from genetic interaction Ref.1. Source: PomBase
Cellular_component	cytosol Inferred from direct assay Ref.3. Source: PomBase nucleus Inferred from direct assay Ref.3. Source: PomBase
Molecular_function	phosphoprotein phosphatase activity Inferred from direct assay Ref.1. Source: PomBase protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 138	138	Dual specificity phosphatase ibp1		PRO_0000256254
Regions
	Domain	19 – 133	115	Rhodanese
Sites
	Active site	70	1	Phosphocysteine intermediate By similarity
Experimental info
	Mutagenesis	70	1	C → S: No catalytic activity. Ref.1
	Mutagenesis	73	1	S → A: No catalytic activity. Ref.1
	Mutagenesis	76	1	R → A: No catalytic activity. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8WZK3 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 48B69E211539AECF	FASTA	138	15,778
	10 20 30 40 50 60 MSTLSYVSPD ALKGWLMESP NEISIIDVRD YDYEGERIPG SVRIPSDTFL ASVDQHVDDL 70 80 90 100 110 120 MKKRSLIVHC TYSQVRGPKA ARVLSEILRN RITESKEKLS LSQKEKLFQN LPTVYILHGG 130 FSAWKRRYGG QQGLIEYD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ibp1p, a novel Cdc25-related phosphatase, suppresses Schizosaccharomyces pombe hsk1 (cdc7)." Snaith H.A., Marlett J., Forsburg S.L. Curr. Genet. 44:38-48(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-70; SER-73 AND ARG-76.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAB46700.1.
PIR	T40714.
RefSeq	NP_595247.1. NM_001021153.2.
3D structure databases
ProteinModelPortal	Q8WZK3.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4701059.
STRING	4896.SPBC839.07-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC839.07.1; SPBC839.07.1:pep; SPBC839.07.
GeneID	2541214.
KEGG	spo:SPBC839.07.
Organism-specific databases
PomBase	SPBC839.07.
Phylogenomic databases
eggNOG	COG0607.
HOGENOM	HOG000162894.
OMA	RITESKE.
OrthoDB	EOG43BQZC.
Family and domain databases
Gene3D	3.40.250.10. 1 hit.
InterPro	IPR001763. Rhodanese-like_dom. [Graphical view]
Pfam	PF00581. Rhodanese. 1 hit. [Graphical view]
SMART	SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20802326.

Entry information

Entry name

IBP1_SCHPO

Accession

Primary (citable) accession number: Q8WZK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	March 1, 2002
Last modified:	May 29, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program