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Q8WZJ7

- C1TC_SCHPO

UniProt

Q8WZJ7 - C1TC_SCHPO

Protein

C-1-tetrahydrofolate synthase, cytoplasmic

Gene

SPBC839.16

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.
    5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.
    ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei193 – 1931NADPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi168 – 1703NADPBy similarity
    Nucleotide bindingi374 – 3818ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. formate-tetrahydrofolate ligase activity Source: PomBase
    3. methenyltetrahydrofolate cyclohydrolase activity Source: PomBase
    4. methylenetetrahydrofolate dehydrogenase (NADP+) activity Source: PomBase
    5. single-stranded DNA binding Source: PomBase

    GO - Biological processi

    1. 10-formyltetrahydrofolate biosynthetic process Source: PomBase
    2. folic acid biosynthetic process Source: PomBase
    3. histidine biosynthetic process Source: UniProtKB-KW
    4. methionine biosynthetic process Source: UniProtKB-KW
    5. one-carbon metabolic process Source: PomBase
    6. purine nucleobase biosynthetic process Source: PomBase
    7. purine nucleotide biosynthetic process Source: UniProtKB-KW
    8. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase, Ligase, Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, NADP, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    C-1-tetrahydrofolate synthase, cytoplasmic
    Short name:
    C1-THF synthase
    Including the following 3 domains:
    Methylenetetrahydrofolate dehydrogenase (EC:1.5.1.5)
    Methenyltetrahydrofolate cyclohydrolase (EC:3.5.4.9)
    Formyltetrahydrofolate synthetase (EC:6.3.4.3)
    Gene namesi
    ORF Names:SPBC839.16
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC839.16.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosol Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 937937C-1-tetrahydrofolate synthase, cytoplasmicPRO_0000315626Add
    BLAST

    Proteomic databases

    MaxQBiQ8WZJ7.
    PaxDbiQ8WZJ7.
    PRIDEiQ8WZJ7.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi277731. 2 interactions.
    MINTiMINT-4700994.
    STRINGi4896.SPBC839.16-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8WZJ7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 309309Methylenetetrahydrofolate dehydrogenase and cyclohydrolaseAdd
    BLAST
    Regioni50 – 545Substrate bindingBy similarity
    Regioni97 – 993Substrate bindingBy similarity
    Regioni268 – 2725Substrate bindingBy similarity
    Regioni310 – 937628Formyltetrahydrofolate synthetaseAdd
    BLAST

    Domaini

    This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.

    Sequence similaritiesi

    In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.Curated
    In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.Curated

    Phylogenomic databases

    eggNOGiCOG0190.
    HOGENOMiHOG000040280.
    KOiK00288.
    OMAiFAPLMFK.
    OrthoDBiEOG7K0ZMJ.
    PhylomeDBiQ8WZJ7.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    3.40.50.720. 1 hit.
    HAMAPiMF_01543. FTHFS.
    MF_01576. THF_DHG_CYH.
    InterProiIPR000559. Formate_THF_ligase.
    IPR020628. Formate_THF_ligase_CS.
    IPR016040. NAD(P)-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR000672. THF_DH/CycHdrlase.
    IPR020630. THF_DH/CycHdrlase_cat_dom.
    IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
    [Graphical view]
    PfamiPF01268. FTHFS. 1 hit.
    PF00763. THF_DHG_CYH. 1 hit.
    PF02882. THF_DHG_CYH_C. 1 hit.
    [Graphical view]
    PRINTSiPR00085. THFDHDRGNASE.
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS00721. FTHFS_1. 1 hit.
    PS00722. FTHFS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8WZJ7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALLLEGTSL ARKVREELRE QISSIKSVDP YFNVSLKIIQ VGGREDSNVY    50
    VRMKTRAANE AGISCEHVNF PEDITEYDLL LAIKGFNEDP TVHGIIVQLP 100
    LPAHINEQII TEAVAPEKDV DGFCETNLGK LTKREGQPLF TACTPKGIMC 150
    ILKHYGINVQ GKHAVVIGRS NIVGRPMSIL LEKANATVTL CHSKTESIAD 200
    IVRTADIVVA AIGIPHFVKA DWLKKGVVAI DVGINSIPDA TKKSGYRLTG 250
    DIDFENAKEV ASAITPVPGS VGPMTVAMLL QNVVESAVRF RKMSRKRKPT 300
    LLPLKLQTPV PSDIEIARSQ TPKNIGDLAS EIGIAKSELE FYGSHKAKVN 350
    LEILQRLAHR RDGHYVVVTG ITPTPFGEGK STLTAGLVQA LSNLDKLAIA 400
    CVRQPSQGPT FGIKGGAAGG GYSQFIPMEE FNLHLTGDIH AITAATNLLA 450
    AAIDTRMFHE NTQSDAALYK RLTLVKGNKR EFAPVMFRRL KKLGIDKTNP 500
    EELTEEEQRK FARLDIEPST ISWNRTLDVN DRFLRKITIG ENPTEKGFTR 550
    QTGFDLSVAS ECMSVLALAT DLKDMRERLG RMVVASNKSG EPVTADDLGV 600
    GGALTVLLKD AIKPTLMQTL EGTPALVHAG PFANISIGAS SILADRIALK 650
    LAGTEVDEDA KKEAGYVVTE AGFASDIGME KFFNIKCRTS GLKPDAIVIV 700
    ATVQALKLHG GGPPVGPGKP IPEVYKREDV DLVRKGCANL AKHISNARKY 750
    GLPVVVAINK FSSDSPNEIS AIREEALAAG ATDAVDSNHW AEGGKGALGV 800
    ARALINACEN VDSEFRLLYD VHEPIEKKIE IIAKEMYGAD GIELSPLAKE 850
    RLETFTKQGY NNLPICIAKT QYSLSHDPDL KGAPTNFTVP IRDMRLSAGA 900
    GFIYPLAAAI STIPGLPTKP AYYNIDIAEN GDIVGLS 937
    Length:937
    Mass (Da):101,203
    Last modified:March 1, 2002 - v1
    Checksum:i70FF8700FD023C90
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAB46709.1.
    PIRiT40723.
    RefSeqiNP_595256.1. NM_001021162.2.

    Genome annotation databases

    EnsemblFungiiSPBC839.16.1; SPBC839.16.1:pep; SPBC839.16.
    GeneIDi2541217.
    KEGGispo:SPBC839.16.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAB46709.1 .
    PIRi T40723.
    RefSeqi NP_595256.1. NM_001021162.2.

    3D structure databases

    ProteinModelPortali Q8WZJ7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277731. 2 interactions.
    MINTi MINT-4700994.
    STRINGi 4896.SPBC839.16-1.

    Proteomic databases

    MaxQBi Q8WZJ7.
    PaxDbi Q8WZJ7.
    PRIDEi Q8WZJ7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC839.16.1 ; SPBC839.16.1:pep ; SPBC839.16 .
    GeneIDi 2541217.
    KEGGi spo:SPBC839.16.

    Organism-specific databases

    PomBasei SPBC839.16.

    Phylogenomic databases

    eggNOGi COG0190.
    HOGENOMi HOG000040280.
    KOi K00288.
    OMAi FAPLMFK.
    OrthoDBi EOG7K0ZMJ.
    PhylomeDBi Q8WZJ7.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .

    Miscellaneous databases

    NextBioi 20802329.
    PROi Q8WZJ7.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    3.40.50.720. 1 hit.
    HAMAPi MF_01543. FTHFS.
    MF_01576. THF_DHG_CYH.
    InterProi IPR000559. Formate_THF_ligase.
    IPR020628. Formate_THF_ligase_CS.
    IPR016040. NAD(P)-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR000672. THF_DH/CycHdrlase.
    IPR020630. THF_DH/CycHdrlase_cat_dom.
    IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
    [Graphical view ]
    Pfami PF01268. FTHFS. 1 hit.
    PF00763. THF_DHG_CYH. 1 hit.
    PF02882. THF_DHG_CYH_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00085. THFDHDRGNASE.
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS00721. FTHFS_1. 1 hit.
    PS00722. FTHFS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiC1TC_SCHPO
    AccessioniPrimary (citable) accession number: Q8WZJ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3