Q8WZ79 (DNS2B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyribonuclease-2-beta
EC=3.1.22.1

Alternative name(s):
DNase II-like acid DNase
DNase2-like acid DNase
Deoxyribonuclease II beta
Short name=DNase II beta
Endonuclease DLAD

Gene names

Name:	DNASE2B
Synonyms:	DLAD

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes DNA under acidic conditions. Does not require divalent cations for activity. Participates in the degradation of nuclear DNA during lens cell differentiation. Ref.1 Ref.5
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.
Subcellular location	Lysosome Probable.
Tissue specificity	Highly expressed in the eye lens and in salivary gland. Detected at lower levels in lung, prostate and lymph node. Isoform 2 is lung specific. Ref.1 Ref.2 Ref.5
Sequence similarities	Belongs to the DNase II family.
Sequence caution	The sequence AAF76893.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Lysosome
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA metabolic process Inferred from electronic annotation. Source: InterPro nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	extracellular region Inferred from electronic annotation. Source: Compara lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	deoxyribonuclease II activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8WZ79-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8WZ79-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-208: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 361	334	Deoxyribonuclease-2-beta		PRO_0000007295
Amino acid modifications
	Glycosylation	81	1	N-linked (GlcNAc...) Potential
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Glycosylation	119	1	N-linked (GlcNAc...) Potential
	Glycosylation	278	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 208	208	Missing in isoform 2.		VSP_009812
	Natural variant	3	1	Q → H. Corresponds to variant rs3738573 [ dbSNP | Ensembl ].		VAR_059250
	Natural variant	47	1	K → R. Corresponds to variant rs3754274 [ dbSNP | Ensembl ].		VAR_048872
	Natural variant	51	1	R → K. Corresponds to variant rs3754274 [ dbSNP | Ensembl ].		VAR_059251

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: C6FDD3F58F62CAC0	FASTA	361	41,713
	10 20 30 40 50 60 MKQKMMARLL RTSFALLFLG LFGVLGAATI SCRNEEGKAV DWFTFYKLPK RQNKESGETG 70 80 90 100 110 120 LEYLYLDSTT RSWRKSEQLM NDTKSVLGRT LQQLYEAYAS KSNNTAYLIY NDGVPKPVNY 130 140 150 160 170 180 SRKYGHTKGL LLWNRVQGFW LIHSIPQFPP IPEEGYDYPP TGRRNGQSGI CITFKYNQYE 190 200 210 220 230 240 AIDSQLLVCN PNVYSCSIPA TFHQELIHMP QLCTRASSSE IPGRLLTTLQ SAQGQKFLHF 250 260 270 280 290 300 AKSDSFLDDI FAAWMAQRLK THLLTETWQR KRQELPSNCS LPYHVYNIKA IKLSRHSYFS 310 320 330 340 350 360 SYQDHAKWCI SQKGTKNRWT CIGDLNRSPH QAFRSGGFIC TQNWQIYQAF QGLVLYYESC K
	Isoform 2 [UniParc]. Checksum: ABF4ED379AFA9FCF	FASTA	153	17,816
	10 20 30 40 50 60 MPQLCTRASS SEIPGRLLTT LQSAQGQKFL HFAKSDSFLD DIFAAWMAQR LKTHLLTETW 70 80 90 100 110 120 QRKRQELPSN CSLPYHVYNI KAIKLSRHSY FSSYQDHAKW CISQKGTKNR WTCIGDLNRS 130 140 150 PHQAFRSGGF ICTQNWQIYQ AFQGLVLYYE SCK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the DLAD/Dlad genes, which lie head-to-head with the genes for urate oxidase." Shiokawa D., Tanuma S. Biochem. Biophys. Res. Commun. 288:1119-1128(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY. Tissue: Lung.
[2]	"The cloning, genomic structure, localization, and expression of human deoxyribonuclease IIbeta." Krieser R.J., MacLea K.S., Park J.P., Eastman A. Gene 269:205-216(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Nuclear cataract caused by a lack of DNA degradation in the mouse eye lens." Nishimoto S., Kawane K., Watanabe-Fukunaga R., Fukuyama H., Ohsawa Y., Uchiyama Y., Hashida N., Ohguro N., Tano Y., Morimoto T., Fukuda Y., Nagata S. Nature 424:1071-1074(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF333389 mRNA. Translation: AAL34448.1. AF334602 Genomic DNA. Translation: AAL34449.1. AF274571 mRNA. Translation: AAF76893.1. Different initiation. AL359273 Genomic DNA. Translation: CAH73126.1. AL359273 Genomic DNA. Translation: CAH73127.1. CH471097 Genomic DNA. Translation: EAW73238.1. CH471097 Genomic DNA. Translation: EAW73239.1.
IPI	IPI00072376. IPI00103879.
RefSeq	NP_067056.2. NM_021233.2. NP_490649.1. NM_058248.1.
UniGene	Hs.129142.
3D structure databases
ProteinModelPortal	Q8WZ79.
ModBase	Search...
PTM databases
PhosphoSite	Q8WZ79.
Polymorphism databases
DMDM	46395921.
Proteomic databases
PaxDb	Q8WZ79.
PRIDE	Q8WZ79.
Protocols and materials databases
DNASU	58511.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000370662; ENSP00000359696; ENSG00000137976. ENST00000370665; ENSP00000359699; ENSG00000137976.
GeneID	58511.
KEGG	hsa:58511.
UCSC	uc001djt.1. human. uc001dju.1. human.
Organism-specific databases
CTD	58511.
GeneCards	GC01P084864.
HGNC	HGNC:28875. DNASE2B.
MIM	608057. gene.
neXtProt	NX_Q8WZ79.
PharmGKB	PA134993904.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG145330.
HOGENOM	HOG000261682.
HOVERGEN	HBG051387.
InParanoid	Q8WZ79.
KO	K01158.
OMA	SSYQDHA.
OrthoDB	EOG4MGS7K.
PhylomeDB	Q8WZ79.
Enzyme and pathway databases
BRENDA	3.1.22.1. 2681.
Gene expression databases
ArrayExpress	Q8WZ79.
Bgee	Q8WZ79.
CleanEx	HS_DNASE2B.
Genevestigator	Q8WZ79.
GermOnline	ENSG00000137976. Homo sapiens.
Family and domain databases
InterPro	IPR004947. DNase_II. [Graphical view]
PANTHER	PTHR10858. PTHR10858. 1 hit.
Pfam	PF03265. DNase_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	58511.
NextBio	65041.
SOURCE	Search...

Entry information

Entry name

DNS2B_HUMAN

Accession

Primary (citable) accession number: Q8WZ79
Secondary accession number(s): Q5VXD0 , Q5VXD1, Q8WZ80, Q9NQW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	March 1, 2002
Last modified:	April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.