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Q8WZ79 (DNS2B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxyribonuclease-2-beta

EC=3.1.22.1
Alternative name(s):
DNase II-like acid DNase
DNase2-like acid DNase
Deoxyribonuclease II beta
Short name=DNase II beta
Endonuclease DLAD
Gene names
Name:DNASE2B
Synonyms:DLAD
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes DNA under acidic conditions. Does not require divalent cations for activity. Participates in the degradation of nuclear DNA during lens cell differentiation. Ref.1 Ref.5

Catalytic activity

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.

Subcellular location

Lysosome Probable.

Tissue specificity

Highly expressed in the eye lens and in salivary gland. Detected at lower levels in lung, prostate and lymph node. Isoform 2 is lung specific. Ref.1 Ref.2 Ref.5

Sequence similarities

Belongs to the DNase II family.

Sequence caution

The sequence AAF76893.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentLysosome
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
   Molecular functionEndonuclease
Hydrolase
Nuclease
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: Ensembl

lysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondeoxyribonuclease II activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8WZ79-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8WZ79-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-208: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 361334Deoxyribonuclease-2-beta
PRO_0000007295

Amino acid modifications

Glycosylation811N-linked (GlcNAc...) Potential
Glycosylation1031N-linked (GlcNAc...) Potential
Glycosylation1191N-linked (GlcNAc...) Potential
Glycosylation2781N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 208208Missing in isoform 2.
VSP_009812
Natural variant31Q → H.
Corresponds to variant rs3738573 [ dbSNP | Ensembl ].
VAR_059250
Natural variant471K → R.
Corresponds to variant rs3754274 [ dbSNP | Ensembl ].
VAR_048872
Natural variant511R → K.
Corresponds to variant rs3754274 [ dbSNP | Ensembl ].
VAR_059251

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: C6FDD3F58F62CAC0

FASTA36141,713
        10         20         30         40         50         60 
MKQKMMARLL RTSFALLFLG LFGVLGAATI SCRNEEGKAV DWFTFYKLPK RQNKESGETG 

        70         80         90        100        110        120 
LEYLYLDSTT RSWRKSEQLM NDTKSVLGRT LQQLYEAYAS KSNNTAYLIY NDGVPKPVNY 

       130        140        150        160        170        180 
SRKYGHTKGL LLWNRVQGFW LIHSIPQFPP IPEEGYDYPP TGRRNGQSGI CITFKYNQYE 

       190        200        210        220        230        240 
AIDSQLLVCN PNVYSCSIPA TFHQELIHMP QLCTRASSSE IPGRLLTTLQ SAQGQKFLHF 

       250        260        270        280        290        300 
AKSDSFLDDI FAAWMAQRLK THLLTETWQR KRQELPSNCS LPYHVYNIKA IKLSRHSYFS 

       310        320        330        340        350        360 
SYQDHAKWCI SQKGTKNRWT CIGDLNRSPH QAFRSGGFIC TQNWQIYQAF QGLVLYYESC 


K 

« Hide

Isoform 2 [UniParc].

Checksum: ABF4ED379AFA9FCF
Show »

FASTA15317,816

References

« Hide 'large scale' references
[1]"Isolation and characterization of the DLAD/Dlad genes, which lie head-to-head with the genes for urate oxidase."
Shiokawa D., Tanuma S.
Biochem. Biophys. Res. Commun. 288:1119-1128(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY.
Tissue: Lung.
[2]"The cloning, genomic structure, localization, and expression of human deoxyribonuclease IIbeta."
Krieser R.J., MacLea K.S., Park J.P., Eastman A.
Gene 269:205-216(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Nuclear cataract caused by a lack of DNA degradation in the mouse eye lens."
Nishimoto S., Kawane K., Watanabe-Fukunaga R., Fukuyama H., Ohsawa Y., Uchiyama Y., Hashida N., Ohguro N., Tano Y., Morimoto T., Fukuda Y., Nagata S.
Nature 424:1071-1074(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF333389 mRNA. Translation: AAL34448.1.
AF334602 Genomic DNA. Translation: AAL34449.1.
AF274571 mRNA. Translation: AAF76893.1. Different initiation.
AL359273 Genomic DNA. Translation: CAH73126.1.
AL359273 Genomic DNA. Translation: CAH73127.1.
CH471097 Genomic DNA. Translation: EAW73238.1.
CH471097 Genomic DNA. Translation: EAW73239.1.
RefSeqNP_067056.2. NM_021233.2.
NP_490649.1. NM_058248.1.
UniGeneHs.129142.

3D structure databases

ProteinModelPortalQ8WZ79.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121838. 2 interactions.
STRING9606.ENSP00000359699.

PTM databases

PhosphoSiteQ8WZ79.

Polymorphism databases

DMDM46395921.

Proteomic databases

PaxDbQ8WZ79.
PRIDEQ8WZ79.

Protocols and materials databases

DNASU58511.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000370662; ENSP00000359696; ENSG00000137976. [Q8WZ79-2]
ENST00000370665; ENSP00000359699; ENSG00000137976. [Q8WZ79-1]
GeneID58511.
KEGGhsa:58511.
UCSCuc001djt.1. human. [Q8WZ79-1]
uc001dju.1. human. [Q8WZ79-2]

Organism-specific databases

CTD58511.
GeneCardsGC01P084864.
HGNCHGNC:28875. DNASE2B.
MIM608057. gene.
neXtProtNX_Q8WZ79.
PharmGKBPA134993904.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG145330.
HOGENOMHOG000261682.
HOVERGENHBG051387.
InParanoidQ8WZ79.
KOK01158.
OMASSYQDHA.
OrthoDBEOG7F5122.
PhylomeDBQ8WZ79.
TreeFamTF314536.

Enzyme and pathway databases

BRENDA3.1.22.1. 2681.

Gene expression databases

ArrayExpressQ8WZ79.
BgeeQ8WZ79.
CleanExHS_DNASE2B.
GenevestigatorQ8WZ79.

Family and domain databases

InterProIPR004947. DNase_II.
[Graphical view]
PANTHERPTHR10858. PTHR10858. 1 hit.
PfamPF03265. DNase_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi58511.
NextBio65041.
PROQ8WZ79.
SOURCESearch...

Entry information

Entry nameDNS2B_HUMAN
AccessionPrimary (citable) accession number: Q8WZ79
Secondary accession number(s): Q5VXD0 expand/collapse secondary AC list , Q5VXD1, Q8WZ80, Q9NQW3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: March 1, 2002
Last modified: April 16, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM