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Q8WZ75 (ROBO4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Roundabout homolog 4
Alternative name(s):
Magic roundabout
Gene names
Name:ROBO4
ORF Names:UNQ421/PRO3674
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1007 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins By similarity.

Subunit structure

Interacts with SLIT2 and ENAH By similarity.

Tissue specificity

Specifically expressed in endothelial cells. Expressed at sites of angiogenesis in different tumor types. Ref.1

Sequence similarities

Belongs to the immunoglobulin superfamily. ROBO family.

Contains 2 fibronectin type-III domains.

Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Sequence caution

The sequence AAH64643.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAA91382.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAB15082.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAB55411.1 differs from that shown. Reason: Frameshift at position 706.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8WZ75-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8WZ75-2)

The sequence of this isoform differs from the canonical sequence as follows:
     932-933: DA → GM
     934-1007: Missing.
Isoform 3 (identifier: Q8WZ75-3)

The sequence of this isoform differs from the canonical sequence as follows:
     24-133: Missing.
     813-1007: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 1007980Roundabout homolog 4
PRO_0000031040

Regions

Domain32 – 131100Ig-like C2-type 1
Domain137 – 22488Ig-like C2-type 2
Domain248 – 34598Fibronectin type-III 1
Domain347 – 44296Fibronectin type-III 2
Compositional bias715 – 78470Pro/Ser-rich

Amino acid modifications

Glycosylation2461N-linked (GlcNAc...) (complex) Ref.5 Ref.6
Glycosylation3601N-linked (GlcNAc...) Potential
Glycosylation3891N-linked (GlcNAc...) Potential
Glycosylation3961N-linked (GlcNAc...) Potential
Glycosylation6801N-linked (GlcNAc...) Potential
Disulfide bond53 ↔ 114 Potential
Disulfide bond158 ↔ 207 Potential

Natural variations

Alternative sequence24 – 133110Missing in isoform 3.
VSP_010654
Alternative sequence813 – 1007195Missing in isoform 3.
VSP_010657
Alternative sequence932 – 9332DA → GM in isoform 2.
VSP_010658
Alternative sequence934 – 100774Missing in isoform 2.
VSP_010659
Natural variant541Q → R.
Corresponds to variant rs59648931 [ dbSNP | Ensembl ].
VAR_062146
Natural variant1031Q → P.
Corresponds to variant rs4995424 [ dbSNP | Ensembl ].
VAR_053643
Natural variant6691R → Q.
Corresponds to variant rs4408324 [ dbSNP | Ensembl ].
VAR_053644

Experimental info

Sequence conflict1751G → E in BAB55411. Ref.3
Sequence conflict4981H → L in BAA91382. Ref.3
Sequence conflict6521Q → P in BAB55411. Ref.3
Sequence conflict6621G → D in BAB84989. Ref.3
Sequence conflict8601P → L in BAB15082. Ref.3
Sequence conflict8641L → F in BAB15082. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: E43F246C59BE1415

FASTA1,007107,457
        10         20         30         40         50         60 
MGSGGDSLLG GRGSLPLLLL LIMGGMAQDS PPQILVHPQD QLFQGPGPAR MSCQASGQPP 

        70         80         90        100        110        120 
PTIRWLLNGQ PLSMVPPDPH HLLPDGTLLL LQPPARGHAH DGQALSTDLG VYTCEASNRL 

       130        140        150        160        170        180 
GTAVSRGARL SVAVLREDFQ IQPRDMVAVV GEQFTLECGP PWGHPEPTVS WWKDGKPLAL 

       190        200        210        220        230        240 
QPGRHTVSGG SLLMARAEKS DEGTYMCVAT NSAGHRESRA ARVSIQEPQD YTEPVELLAV 

       250        260        270        280        290        300 
RIQLENVTLL NPDPAEGPKP RPAVWLSWKV SGPAAPAQSY TALFRTQTAP GGQGAPWAEE 

       310        320        330        340        350        360 
LLAGWQSAEL GGLHWGQDYE FKVRPSSGRA RGPDSNVLLL RLPEKVPSAP PQEVTLKPGN 

       370        380        390        400        410        420 
GTVFVSWVPP PAENHNGIIR GYQVWSLGNT SLPPANWTVV GEQTQLEIAT HMPGSYCVQV 

       430        440        450        460        470        480 
AAVTGAGAGE PSRPVCLLLE QAMERATQEP SEHGPWTLEQ LRATLKRPEV IATCGVALWL 

       490        500        510        520        530        540 
LLLGTAVCIH RRRRARVHLG PGLYRYTSED AILKHRMDHS DSQWLADTWR STSGSRDLSS 

       550        560        570        580        590        600 
SSSLSSRLGA DARDPLDCRR SLLSWDSRSP GVPLLPDTST FYGSLIAELP SSTPARPSPQ 

       610        620        630        640        650        660 
VPAVRRLPPQ LAQLSSPCSS SDSLCSRRGL SSPRLSLAPA EAWKAKKKQE LQHANSSPLL 

       670        680        690        700        710        720 
RGSHSLELRA CELGNRGSKN LSQSPGAVPQ ALVAWRALGP KLLSSSNELV TRHLPPAPLF 

       730        740        750        760        770        780 
PHETPPTQSQ QTQPPVAPQA PSSILLPAAP IPILSPCSPP SPQASSLSGP SPASSRLSSS 

       790        800        810        820        830        840 
SLSSLGEDQD SVLTPEEVAL CLELSEGEET PRNSVSPMPR APSPPTTYGY ISVPTASEFT 

       850        860        870        880        890        900 
DMGRTGGGVG PKGGVLLCPP RPCLTPTPSE GSLANGWGSA SEDNAASARA SLVSSSDGSF 

       910        920        930        940        950        960 
LADAHFARAL AVAVDSFGFG LEPREADCVF IDASSPPSPR DEIFLTPNLS LPLWEWRPDW 

       970        980        990       1000 
LEDMEVSHTQ RLGRGMPPWP PDSQISSQRS QLHCRMPKAG ASPVDYS 

« Hide

Isoform 2 [UniParc].

Checksum: 1939F45D34AB81FA
Show »

FASTA93398,994
Isoform 3 [UniParc].

Checksum: 3988CDE4BF9FEE9B
Show »

FASTA70275,282

References

« Hide 'large scale' references
[1]"Magic roundabout is a new member of the roundabout receptor family that is endothelial specific and expressed at sites of active angiogenesis."
Huminiecki L., Gorn M., Suchting S., Poulsom R., Bicknell R.
Genomics 79:547-552(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-1007 (ISOFORM 1).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Tissue: Adipose tissue, Brain and Colon.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain and Ovary.
[5]"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-246.
Tissue: Plasma.
[6]"A strategy for precise and large scale identification of core fucosylated glycoproteins."
Jia W., Lu Z., Fu Y., Wang H.P., Wang L.H., Chi H., Yuan Z.F., Zheng Z.B., Song L.N., Han H.H., Liang Y.M., Wang J.L., Cai Y., Zhang Y.K., Deng Y.L., Ying W.T., He S.M., Qian X.H.
Mol. Cell. Proteomics 8:913-923(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT ASN-246.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF361473 mRNA. Translation: AAL31867.1.
AY358083 mRNA. Translation: AAQ88450.1.
AK000805 mRNA. Translation: BAA91382.1. Different initiation.
AK025195 mRNA. Translation: BAB15082.1. Different initiation.
AK027852 mRNA. Translation: BAB55411.1. Frameshift.
AK074163 mRNA. Translation: BAB84989.1.
AK289769 mRNA. Translation: BAF82458.1.
BC039602 mRNA. Translation: AAH39602.1.
BC064643 mRNA. Translation: AAH64643.1. Different initiation.
BC111562 mRNA. Translation: AAI11563.1.
BC111748 mRNA. Translation: AAI11749.1.
CCDSCCDS8455.1. [Q8WZ75-1]
RefSeqNP_061928.4. NM_019055.5. [Q8WZ75-1]
UniGeneHs.524121.

3D structure databases

ProteinModelPortalQ8WZ75.
SMRQ8WZ75. Positions 28-441.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120025. 4 interactions.
IntActQ8WZ75. 5 interactions.
STRING9606.ENSP00000304945.

Protein family/group databases

MEROPSI43.001.

PTM databases

PhosphoSiteQ8WZ75.

Polymorphism databases

DMDM49036490.

Proteomic databases

PaxDbQ8WZ75.
PRIDEQ8WZ75.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000306534; ENSP00000304945; ENSG00000154133. [Q8WZ75-1]
GeneID54538.
KEGGhsa:54538.
UCSCuc001qbg.3. human. [Q8WZ75-1]
uc001qbh.2. human. [Q8WZ75-3]
uc001qbi.3. human. [Q8WZ75-2]

Organism-specific databases

CTD54538.
GeneCardsGC11M124753.
HGNCHGNC:17985. ROBO4.
MIM607528. gene.
neXtProtNX_Q8WZ75.
PharmGKBPA34622.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG325715.
HOVERGENHBG061836.
InParanoidQ8WZ75.
KOK06784.
OMAQILVHPQ.
OrthoDBEOG7FFMR3.
PhylomeDBQ8WZ75.
TreeFamTF351053.

Gene expression databases

ArrayExpressQ8WZ75.
BgeeQ8WZ75.
CleanExHS_ROBO4.
GenevestigatorQ8WZ75.

Family and domain databases

Gene3D2.60.40.10. 4 hits.
InterProIPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
[Graphical view]
PfamPF00041. fn3. 1 hit.
PF07679. I-set. 2 hits.
[Graphical view]
SMARTSM00060. FN3. 2 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
SUPFAMSSF49265. SSF49265. 1 hit.
PROSITEPS50853. FN3. 2 hits.
PS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiROBO4.
GenomeRNAi54538.
NextBio56970.
PROQ8WZ75.
SOURCESearch...

Entry information

Entry nameROBO4_HUMAN
AccessionPrimary (citable) accession number: Q8WZ75
Secondary accession number(s): A8K154 expand/collapse secondary AC list , Q14DU7, Q8TEG1, Q96JV6, Q9H718, Q9NWJ8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM