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Q8WZ75

- ROBO4_HUMAN

UniProt

Q8WZ75 - ROBO4_HUMAN

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Protein

Roundabout homolog 4

Gene
ROBO4, UNQ421/PRO3674
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins By similarity.

GO - Molecular functioni

  1. receptor activity Source: UniProtKB

GO - Biological processi

  1. angiogenesis Source: UniProtKB
  2. cell differentiation Source: UniProtKB-KW
  3. negative regulation of cell migration Source: Ensembl
  4. regulation of cell migration Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Receptor

Keywords - Biological processi

Angiogenesis, Differentiation

Protein family/group databases

MEROPSiI43.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Roundabout homolog 4
Alternative name(s):
Magic roundabout
Gene namesi
Name:ROBO4
ORF Names:UNQ421/PRO3674
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:17985. ROBO4.

Subcellular locationi

GO - Cellular componenti

  1. external side of plasma membrane Source: Ensembl
  2. extracellular vesicular exosome Source: UniProt
  3. integral component of membrane Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34622.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed predictionAdd
BLAST
Chaini28 – 1007980Roundabout homolog 4PRO_0000031040Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi53 ↔ 114 Reviewed prediction
Disulfide bondi158 ↔ 207 Reviewed prediction
Glycosylationi246 – 2461N-linked (GlcNAc...) (complex)2 Publications
Glycosylationi360 – 3601N-linked (GlcNAc...) Reviewed prediction
Glycosylationi389 – 3891N-linked (GlcNAc...) Reviewed prediction
Glycosylationi396 – 3961N-linked (GlcNAc...) Reviewed prediction
Glycosylationi680 – 6801N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8WZ75.
PRIDEiQ8WZ75.

PTM databases

PhosphoSiteiQ8WZ75.

Expressioni

Tissue specificityi

Specifically expressed in endothelial cells. Expressed at sites of angiogenesis in different tumor types.1 Publication

Gene expression databases

ArrayExpressiQ8WZ75.
BgeeiQ8WZ75.
CleanExiHS_ROBO4.
GenevestigatoriQ8WZ75.

Interactioni

Subunit structurei

Interacts with SLIT2 and ENAH By similarity.

Protein-protein interaction databases

BioGridi120025. 4 interactions.
IntActiQ8WZ75. 5 interactions.
STRINGi9606.ENSP00000304945.

Structurei

3D structure databases

ProteinModelPortaliQ8WZ75.
SMRiQ8WZ75. Positions 28-441.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 131100Ig-like C2-type 1Add
BLAST
Domaini137 – 22488Ig-like C2-type 2Add
BLAST
Domaini248 – 34598Fibronectin type-III 1Add
BLAST
Domaini347 – 44296Fibronectin type-III 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi715 – 78470Pro/Ser-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG325715.
HOVERGENiHBG061836.
InParanoidiQ8WZ75.
KOiK06784.
OMAiQILVHPQ.
OrthoDBiEOG7FFMR3.
PhylomeDBiQ8WZ75.
TreeFamiTF351053.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF00041. fn3. 1 hit.
PF07679. I-set. 2 hits.
[Graphical view]
SMARTiSM00060. FN3. 2 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 2 hits.
PS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8WZ75-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGSGGDSLLG GRGSLPLLLL LIMGGMAQDS PPQILVHPQD QLFQGPGPAR     50
MSCQASGQPP PTIRWLLNGQ PLSMVPPDPH HLLPDGTLLL LQPPARGHAH 100
DGQALSTDLG VYTCEASNRL GTAVSRGARL SVAVLREDFQ IQPRDMVAVV 150
GEQFTLECGP PWGHPEPTVS WWKDGKPLAL QPGRHTVSGG SLLMARAEKS 200
DEGTYMCVAT NSAGHRESRA ARVSIQEPQD YTEPVELLAV RIQLENVTLL 250
NPDPAEGPKP RPAVWLSWKV SGPAAPAQSY TALFRTQTAP GGQGAPWAEE 300
LLAGWQSAEL GGLHWGQDYE FKVRPSSGRA RGPDSNVLLL RLPEKVPSAP 350
PQEVTLKPGN GTVFVSWVPP PAENHNGIIR GYQVWSLGNT SLPPANWTVV 400
GEQTQLEIAT HMPGSYCVQV AAVTGAGAGE PSRPVCLLLE QAMERATQEP 450
SEHGPWTLEQ LRATLKRPEV IATCGVALWL LLLGTAVCIH RRRRARVHLG 500
PGLYRYTSED AILKHRMDHS DSQWLADTWR STSGSRDLSS SSSLSSRLGA 550
DARDPLDCRR SLLSWDSRSP GVPLLPDTST FYGSLIAELP SSTPARPSPQ 600
VPAVRRLPPQ LAQLSSPCSS SDSLCSRRGL SSPRLSLAPA EAWKAKKKQE 650
LQHANSSPLL RGSHSLELRA CELGNRGSKN LSQSPGAVPQ ALVAWRALGP 700
KLLSSSNELV TRHLPPAPLF PHETPPTQSQ QTQPPVAPQA PSSILLPAAP 750
IPILSPCSPP SPQASSLSGP SPASSRLSSS SLSSLGEDQD SVLTPEEVAL 800
CLELSEGEET PRNSVSPMPR APSPPTTYGY ISVPTASEFT DMGRTGGGVG 850
PKGGVLLCPP RPCLTPTPSE GSLANGWGSA SEDNAASARA SLVSSSDGSF 900
LADAHFARAL AVAVDSFGFG LEPREADCVF IDASSPPSPR DEIFLTPNLS 950
LPLWEWRPDW LEDMEVSHTQ RLGRGMPPWP PDSQISSQRS QLHCRMPKAG 1000
ASPVDYS 1007
Length:1,007
Mass (Da):107,457
Last modified:March 1, 2002 - v1
Checksum:iE43F246C59BE1415
GO
Isoform 2 (identifier: Q8WZ75-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     932-933: DA → GM
     934-1007: Missing.

Show »
Length:933
Mass (Da):98,994
Checksum:i1939F45D34AB81FA
GO
Isoform 3 (identifier: Q8WZ75-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     24-133: Missing.
     813-1007: Missing.

Show »
Length:702
Mass (Da):75,282
Checksum:i3988CDE4BF9FEE9B
GO

Sequence cautioni

The sequence BAB55411.1 differs from that shown. Reason: Frameshift at position 706.
The sequence AAH64643.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAA91382.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAB15082.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti54 – 541Q → R.
Corresponds to variant rs59648931 [ dbSNP | Ensembl ].
VAR_062146
Natural varianti103 – 1031Q → P.
Corresponds to variant rs4995424 [ dbSNP | Ensembl ].
VAR_053643
Natural varianti669 – 6691R → Q.
Corresponds to variant rs4408324 [ dbSNP | Ensembl ].
VAR_053644

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei24 – 133110Missing in isoform 3. VSP_010654Add
BLAST
Alternative sequencei813 – 1007195Missing in isoform 3. VSP_010657Add
BLAST
Alternative sequencei932 – 9332DA → GM in isoform 2. VSP_010658
Alternative sequencei934 – 100774Missing in isoform 2. VSP_010659Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti175 – 1751G → E in BAB55411. 1 Publication
Sequence conflicti498 – 4981H → L in BAA91382. 1 Publication
Sequence conflicti652 – 6521Q → P in BAB55411. 1 Publication
Sequence conflicti662 – 6621G → D in BAB84989. 1 Publication
Sequence conflicti860 – 8601P → L in BAB15082. 1 Publication
Sequence conflicti864 – 8641L → F in BAB15082. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF361473 mRNA. Translation: AAL31867.1.
AY358083 mRNA. Translation: AAQ88450.1.
AK000805 mRNA. Translation: BAA91382.1. Different initiation.
AK025195 mRNA. Translation: BAB15082.1. Different initiation.
AK027852 mRNA. Translation: BAB55411.1. Frameshift.
AK074163 mRNA. Translation: BAB84989.1.
AK289769 mRNA. Translation: BAF82458.1.
BC039602 mRNA. Translation: AAH39602.1.
BC064643 mRNA. Translation: AAH64643.1. Different initiation.
BC111562 mRNA. Translation: AAI11563.1.
BC111748 mRNA. Translation: AAI11749.1.
CCDSiCCDS8455.1. [Q8WZ75-1]
RefSeqiNP_061928.4. NM_019055.5. [Q8WZ75-1]
UniGeneiHs.524121.

Genome annotation databases

EnsembliENST00000306534; ENSP00000304945; ENSG00000154133. [Q8WZ75-1]
GeneIDi54538.
KEGGihsa:54538.
UCSCiuc001qbg.3. human. [Q8WZ75-1]
uc001qbh.2. human. [Q8WZ75-3]
uc001qbi.3. human. [Q8WZ75-2]

Polymorphism databases

DMDMi49036490.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF361473 mRNA. Translation: AAL31867.1 .
AY358083 mRNA. Translation: AAQ88450.1 .
AK000805 mRNA. Translation: BAA91382.1 . Different initiation.
AK025195 mRNA. Translation: BAB15082.1 . Different initiation.
AK027852 mRNA. Translation: BAB55411.1 . Frameshift.
AK074163 mRNA. Translation: BAB84989.1 .
AK289769 mRNA. Translation: BAF82458.1 .
BC039602 mRNA. Translation: AAH39602.1 .
BC064643 mRNA. Translation: AAH64643.1 . Different initiation.
BC111562 mRNA. Translation: AAI11563.1 .
BC111748 mRNA. Translation: AAI11749.1 .
CCDSi CCDS8455.1. [Q8WZ75-1 ]
RefSeqi NP_061928.4. NM_019055.5. [Q8WZ75-1 ]
UniGenei Hs.524121.

3D structure databases

ProteinModelPortali Q8WZ75.
SMRi Q8WZ75. Positions 28-441.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 120025. 4 interactions.
IntActi Q8WZ75. 5 interactions.
STRINGi 9606.ENSP00000304945.

Protein family/group databases

MEROPSi I43.001.

PTM databases

PhosphoSitei Q8WZ75.

Polymorphism databases

DMDMi 49036490.

Proteomic databases

PaxDbi Q8WZ75.
PRIDEi Q8WZ75.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000306534 ; ENSP00000304945 ; ENSG00000154133 . [Q8WZ75-1 ]
GeneIDi 54538.
KEGGi hsa:54538.
UCSCi uc001qbg.3. human. [Q8WZ75-1 ]
uc001qbh.2. human. [Q8WZ75-3 ]
uc001qbi.3. human. [Q8WZ75-2 ]

Organism-specific databases

CTDi 54538.
GeneCardsi GC11M124753.
HGNCi HGNC:17985. ROBO4.
MIMi 607528. gene.
neXtProti NX_Q8WZ75.
PharmGKBi PA34622.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG325715.
HOVERGENi HBG061836.
InParanoidi Q8WZ75.
KOi K06784.
OMAi QILVHPQ.
OrthoDBi EOG7FFMR3.
PhylomeDBi Q8WZ75.
TreeFami TF351053.

Miscellaneous databases

GeneWikii ROBO4.
GenomeRNAii 54538.
NextBioi 56970.
PROi Q8WZ75.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8WZ75.
Bgeei Q8WZ75.
CleanExi HS_ROBO4.
Genevestigatori Q8WZ75.

Family and domain databases

Gene3Di 2.60.40.10. 4 hits.
InterProi IPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
[Graphical view ]
Pfami PF00041. fn3. 1 hit.
PF07679. I-set. 2 hits.
[Graphical view ]
SMARTi SM00060. FN3. 2 hits.
SM00408. IGc2. 2 hits.
[Graphical view ]
SUPFAMi SSF49265. SSF49265. 1 hit.
PROSITEi PS50853. FN3. 2 hits.
PS50835. IG_LIKE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Magic roundabout is a new member of the roundabout receptor family that is endothelial specific and expressed at sites of active angiogenesis."
    Huminiecki L., Gorn M., Suchting S., Poulsom R., Bicknell R.
    Genomics 79:547-552(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-1007 (ISOFORM 1).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Tissue: Adipose tissue, Brain and Colon.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain and Ovary.
  5. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
    Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
    J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-246.
    Tissue: Plasma.
  6. Cited for: GLYCOSYLATION AT ASN-246.

Entry informationi

Entry nameiROBO4_HUMAN
AccessioniPrimary (citable) accession number: Q8WZ75
Secondary accession number(s): A8K154
, Q14DU7, Q8TEG1, Q96JV6, Q9H718, Q9NWJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: March 1, 2002
Last modified: September 3, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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