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Q8WZ64 (ARAP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2
Alternative name(s):
Centaurin-delta-1
Short name=Cnt-d1
Protein PARX
Gene names
Name:ARAP2
Synonyms:CENTD1, KIAA0580
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1704 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency By similarity.

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Detected in brain, thymus, lymph node, thyroid, spinal cord, trachea, heart, skeletal muscle, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. Ref.1

Sequence similarities

Contains 1 Arf-GAP domain.

Contains 5 PH domains.

Contains 1 Ras-associating domain.

Contains 1 Rho-GAP domain.

Contains 1 SAM (sterile alpha motif) domain.

Sequence caution

The sequence AAL04166.1 differs from that shown. Reason: Frameshift at position 1579.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 17041704Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2
PRO_0000074212

Regions

Domain6 – 7065SAM
Domain482 – 57493PH 1
Domain587 – 67993PH 2
Domain676 – 811136Arf-GAP
Domain878 – 1003126PH 3
Domain1014 – 1114101PH 4
Domain1116 – 1297182Rho-GAP
Domain1326 – 142095Ras-associating
Domain1434 – 1537104PH 5
Zinc finger700 – 72324C4-type

Amino acid modifications

Modified residue771Phosphotyrosine By similarity

Natural variations

Natural variant3841K → N.
Corresponds to variant rs35468501 [ dbSNP | Ensembl ].
VAR_055530
Natural variant10061F → L.
Corresponds to variant rs35218548 [ dbSNP | Ensembl ].
VAR_055531
Natural variant15231R → Q. Ref.1 Ref.2 Ref.4
Corresponds to variant rs4833069 [ dbSNP | Ensembl ].
VAR_027952

Experimental info

Sequence conflict2271T → I in AAL32459. Ref.2
Sequence conflict5321I → V in AAL32459. Ref.2

Secondary structure

............................... 1704
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8WZ64 [UniParc].

Last modified October 5, 2010. Version 3.
Checksum: A5A13EE08D43A9A0

FASTA1,704193,452
        10         20         30         40         50         60 
MSSVSEVNVD IKDFLMSINL EQYLLHFHES GFTTVKDCAA INDSLLQKIG ISPTGHRRRI 

        70         80         90        100        110        120 
LKQLQIILSK MQDIPIYANV HKTKKNDDPS KDYHVPSSDQ NICIELSNSG SVQTSSPPQL 

       130        140        150        160        170        180 
ETVRKNLEDS DASVERSQYP QSDDKLSPPK RDFPTAEEPH LNLGSLNDSL FGSDNIKIES 

       190        200        210        220        230        240 
LITKKTVDHT VEEQQTEKVK LITENLSKLP NADSECLSFV GCSTSGTNSG NGTNGLLEGS 

       250        260        270        280        290        300 
PPSPFFKFQG EMIVNDLYVP SSPILAPVRS RSKLVSRPSR SFLLRHRPVP EIPGSTKGVS 

       310        320        330        340        350        360 
GSYFRERRNV ATSTEKSVAW QNSNEENSSS IFPYGETFLF QRLENSKKRS IKNEFLTQGE 

       370        380        390        400        410        420 
ALKGEAATAT NSFIIKSSIY DNRKEKISED KVEDIWIPRE DKNNFLIDTA SESEYSTVEE 

       430        440        450        460        470        480 
CFQSLRRKNS KASKSRTQKA LILDSVNRHS YPLSSTSGNA DSSAVSSQAI SPYACFYGAS 

       490        500        510        520        530        540 
AKKVKSGWLD KLSPQGKRMF QKRWVKFDGL SISYYNNEKE MYSKGIIPLS AISTVRVQGD 

       550        560        570        580        590        600 
NKFEVVTTQR TFVFRVEKEE ERNDWISILL NALKSQSLTS QSQAVVTPEK CGYLELRGYK 

       610        620        630        640        650        660 
AKIFTVLSGN SVWLCKNEQD FKSGLGITII PMNVANVKQV DRTVKQSFEI ITPYRSFSFT 

       670        680        690        700        710        720 
AETEKEKQDW IEAVQQSIAE TLSDYEVAEK IWFNESNRSC ADCKAPDPDW ASINLCVVIC 

       730        740        750        760        770        780 
KKCAGQHRSL GPKDSKVRSL KMDASIWSNE LIELFIVIGN KRANDFWAGN LQKDEELHMD 

       790        800        810        820        830        840 
SPVEKRKNFI TQKYKEGKFR KTLLASLTKE ELNKALCAAV VKPDVLETMA LLFSGADVMC 

       850        860        870        880        890        900 
ATGDPVHSTP YLLAKKAGQS LQMEFLYHNK FSDFPQHDIH SEGVLSQESS QSTFLCDFLY 

       910        920        930        940        950        960 
QAPSAASKLS SEKKLLEETN KKWCVLEGGF LSYYENDKST TPNGTININE VICLAIHKED 

       970        980        990       1000       1010       1020 
FYLNTGPIFI FEIYLPSERV FLFGAETSQA QRKWTEAIAK HFVPLFAENL TEADYDLIGQ 

      1030       1040       1050       1060       1070       1080 
LFYKDCHALD QWRKGWFAMD KSSLHFCLQM QEVQGDRMHL RRLQELTIST MVQNGEKLDV 

      1090       1100       1110       1120       1130       1140 
LLLVEKGRTL YIHGHTKLDF TVWHTAIEKA AGTDGNALQD QQLSKNDVPI IVNSCIAFVT 

      1150       1160       1170       1180       1190       1200 
QYGLGCKYIY QKNGDPLHIS ELLESFKKDA RSFKLRAGKH QLEDVTAVLK SFLSDIDDAL 

      1210       1220       1230       1240       1250       1260 
LTKELYPYWI SALDTQDDKE RIKKYGAFIR SLPGVNRATL AAIIEHLYRV QKCSEINHMN 

      1270       1280       1290       1300       1310       1320 
AHNLALVFSS CLFQTKGQTS EEVNVIEDLI NNYVEIFEVK EDQVKQMDIE NSFITKWKDT 

      1330       1340       1350       1360       1370       1380 
QVSQAGDLLI EVYVERKEPD CSIIIRISPV MEAEELTNDI LAIKNIIPTK GDIWATFEVI 

      1390       1400       1410       1420       1430       1440 
ENEELERPLH YKENVLEQVL RWSSLAEPGS AYLVVKRFLT ADTIKHCSDR STLGSIKEGI 

      1450       1460       1470       1480       1490       1500 
LKIKEEPSKI LSGNKFQDRY FVLRDGFLFL YKDVKSSKHD KMFSLSSMKF YRGVKKKMKP 

      1510       1520       1530       1540       1550       1560 
PTSWGLTAYS EKHHWHLCCD SSRTQTEWMT SIFIAQHEYD IWPPAGKERK RSITKNPKIG 

      1570       1580       1590       1600       1610       1620 
GLPLIPIQHE GNATLARKNI ESARAELERL RLSEKCDKES VDSSLKERAS MVAHCLEHKD 

      1630       1640       1650       1660       1670       1680 
DKLRNRPRKH RSFNCLEDTE PEAPLGQPKG HKGLKTLRKT EDRNSKATLD SDHKLPSRVI 

      1690       1700 
EELNVVLQRS RTLPKELQDE QILK 

« Hide

References

« Hide 'large scale' references
[1]"ARAP1: a point of convergence for Arf and Rho signaling."
Miura K., Jacques K.M., Stauffer S., Kubosaki A., Zhu K., Hirsch D.S., Resau J., Zheng Y., Randazzo P.A.
Mol. Cell 9:109-119(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT GLN-1523.
[2]Wong J.A., Chen Z., Marignani P.A., Yu M., Zhao Y., Vallis K.A.
Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLN-1523.
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:31-39(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 74-1704, VARIANT GLN-1523.
Tissue: Brain.
[5]Ohara O., Nagase T., Ishikawa K.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[6]"KIAA0580 as a member (centaurin delta1) of ArfGAP-domain centaurin family."
Hong W.
Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 742-1605.
[7]"Solution structure of the N-terminal PH domain and of the SAM domain of human ARAP2."
RIKEN structural genomics initiative (RSGI)
Submitted (NOV-2005) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 1-78 AND 483-584.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY049733 mRNA. Translation: AAL12170.1.
AF439781 mRNA. Translation: AAL32459.1.
AC098827 Genomic DNA. No translation available.
AC104078 Genomic DNA. No translation available.
AC108033 Genomic DNA. Translation: AAY40927.1.
AC109818 Genomic DNA. No translation available.
AC116623 Genomic DNA. No translation available.
AB011152 mRNA. Translation: BAA25506.2.
AF411982 mRNA. Translation: AAL04166.1. Frameshift.
PIRT00342.
RefSeqNP_056045.2. NM_015230.3.
XP_005262695.1. XM_005262638.2.
UniGeneHs.479451.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1X40NMR-A1-78[»]
2CODNMR-A483-584[»]
ProteinModelPortalQ8WZ64.
SMRQ8WZ64. Positions 6-79, 488-584, 590-680, 686-1006, 1128-1289, 1455-1535.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125547. 1 interaction.
IntActQ8WZ64. 3 interactions.
MINTMINT-7969711.
STRING9606.ENSP00000302895.

PTM databases

PhosphoSiteQ8WZ64.

Polymorphism databases

DMDM308153629.

Proteomic databases

PaxDbQ8WZ64.
PRIDEQ8WZ64.

Protocols and materials databases

DNASU116984.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000303965; ENSP00000302895; ENSG00000047365.
GeneID116984.
KEGGhsa:116984.
UCSCuc003gsq.2. human.

Organism-specific databases

CTD116984.
GeneCardsGC04M035950.
H-InvDBHIX0004149.
HGNCHGNC:16924. ARAP2.
HPAHPA035925.
MIM606645. gene.
neXtProtNX_Q8WZ64.
PharmGKBPA164715938.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5347.
HOGENOMHOG000246988.
HOVERGENHBG095947.
InParanoidQ8WZ64.
KOK12490.
OMATQYGLGC.
OrthoDBEOG7F24T4.
PhylomeDBQ8WZ64.
TreeFamTF105769.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressQ8WZ64.
BgeeQ8WZ64.
CleanExHS_ARAP2.
GenevestigatorQ8WZ64.

Family and domain databases

Gene3D1.10.150.50. 1 hit.
1.10.555.10. 1 hit.
2.30.29.30. 4 hits.
InterProIPR001164. ArfGAP.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR000159. Ras-assoc.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
[Graphical view]
PfamPF01412. ArfGap. 1 hit.
PF00169. PH. 4 hits.
PF00788. RA. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
PRINTSPR00405. REVINTRACTNG.
SMARTSM00105. ArfGap. 1 hit.
SM00233. PH. 5 hits.
SM00324. RhoGAP. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMSSF47769. SSF47769. 1 hit.
SSF48350. SSF48350. 1 hit.
PROSITEPS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 4 hits.
PS50200. RA. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8WZ64.
GeneWikiCENTD1.
GenomeRNAi116984.
NextBio80056.
PROQ8WZ64.
SOURCESearch...

Entry information

Entry nameARAP2_HUMAN
AccessionPrimary (citable) accession number: Q8WZ64
Secondary accession number(s): Q4W5D2 expand/collapse secondary AC list , Q7Z2L5, Q96L70, Q96P49, Q9Y4E4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: October 5, 2010
Last modified: April 16, 2014
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM