Q8WZ19 (BACD1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1
Short name=hBACURD1

Alternative name(s):
BTB/POZ domain-containing protein KCTD13
Polymerase delta-interacting protein 1
TNFAIP1-like protein

Gene names

Name:	KCTD13
Synonyms:	BACURD1, PDIP1, POLDIP1
ORF Names:	FKSG86, PP6832

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Ref.9
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Component of the BCR(BACURD1) E3 ubiquitin ligase complex, at least composed of CUL3, KCTD13/BACURD1 and RBX1. Interacts with RHOA; with a preference for RhoA-GDP. Interacts with POLD2 and PCNA. Ref.1 Ref.9
Subcellular location	Nucleus Ref.1.
Tissue specificity	Expressed in a wide variety of tissues. Ref.1
Induction	By TNF and IL6/interleukin-6. Ref.1
Sequence similarities	Belongs to the BACURD family. Contains 1 BTB (POZ) domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from sequence or structural similarity PubMed 15726626. Source: UniProtKB cell migration Inferred from mutant phenotype Ref.9. Source: UniProtKB negative regulation of Rho protein signal transduction Inferred from mutant phenotype Ref.9. Source: UniProtKB positive regulation of DNA replication Inferred from electronic annotation. Source: Compara proteasomal ubiquitin-dependent protein catabolic process Inferred from direct assay Ref.9. Source: UniProtKB protein homooligomerization Inferred from electronic annotation. Source: InterPro protein ubiquitination Inferred from direct assay Ref.9. Source: UniProtKB stress fiber assembly Inferred from mutant phenotype Ref.9. Source: UniProtKB
Cellular_component	Cul3-RING ubiquitin ligase complex Inferred from direct assay Ref.9. Source: UniProtKB nucleus Inferred from direct assay. Source: HPA
Molecular_function	GTP-Rho binding Inferred from direct assay Ref.9. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1		PRO_0000191297
Regions
	Domain	41 – 109	69	BTB
Experimental info
	Mutagenesis	84 – 86	3	VLI → AAA: Abolishes interaction with CUL3 and induces abnormal actin stress fibers. Ref.9
	Sequence conflict	251	1	K → N in AAK27301. Ref.3
	Sequence conflict	328	1	K → R in AAK27301. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8WZ19 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 17A8AC2DBE81EE78	FASTA	329	36,357
	10 20 30 40 50 60 MSAEASGPAA AAAPSLEAPK PSGLEPGPAA YGLKPLTPNS KYVKLNVGGS LHYTTLRTLT 70 80 90 100 110 120 GQDTMLKAMF SGRVEVLTDA GGWVLIDRSG RHFGTILNYL RDGSVPLPES TRELGELLGE 130 140 150 160 170 180 ARYYLVQGLI EDCQLALQQK RETLSPLCLI PMVTSPREEQ QLLASTSKPV VKLLHNRSNN 190 200 210 220 230 240 KYSYTSTSDD NLLKNIELFD KLALRFHGRL LFLKDVLGDE ICCWSFYGQG RKIAEVCCTS 250 260 270 280 290 300 IVYATEKKQT KVEFPEARIF EETLNILIYE TPRGPDPALL EATGGAAGAG GAGRGEDEEN 310 320 REHRVRRIHV RRHITHDERP HGQQIVFKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A tumor necrosis factor alpha- and interleukin 6-inducible protein that interacts with the small subunit of DNA polymerase delta and proliferating cell nuclear antigen." He H., Tan C.-K., Downey K.M., So A.G. Proc. Natl. Acad. Sci. U.S.A. 98:11979-11984(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH PCNA AND POLD2, TISSUE SPECIFICITY, INDUCTION.
[2]	He H., Tan C.-K., Downey K.M., So A.G. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Cloning and characterization of FKSG86, a novel gene encoding a TNFAIP1-like protein." Wang Y.-G., Gong L. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Amygdala.
[6]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[9]	"Cullin mediates degradation of RhoA through evolutionarily conserved BTB adaptors to control actin cytoskeleton structure and cell movement." Chen Y., Yang Z., Meng M., Zhao Y., Dong N., Yan H., Liu L., Ding M., Peng H.B., Shao F. Mol. Cell 35:841-855(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN A BCR (BTB-CUL3-RBX1) E3 UBIQUITIN LIGASE COMPLEX, INTERACTION WITH RHOA, MUTAGENESIS OF 84-VAL--ILE-86.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF401315 mRNA. Translation: AAL14962.2. AY027918 mRNA. Translation: AAK27301.1. AF289573 mRNA. Translation: AAL55757.1. AK289630 mRNA. Translation: BAF82319.1. AC120114 Genomic DNA. No translation available. CH471238 Genomic DNA. Translation: EAW79969.1. BC036228 mRNA. Translation: AAH36228.1.
IPI	IPI00157833.
RefSeq	NP_849194.1. NM_178863.3.
UniGene	Hs.534590.
3D structure databases
ProteinModelPortal	Q8WZ19.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8WZ19. 12 interactions.
MINT	MINT-1458356.
STRING	9606.ENSP00000311202.
PTM databases
PhosphoSite	Q8WZ19.
Polymorphism databases
DMDM	51701604.
Proteomic databases
PaxDb	Q8WZ19.
PRIDE	Q8WZ19.
Protocols and materials databases
DNASU	253980.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000308768; ENSP00000311202; ENSG00000174943. ENST00000568000; ENSP00000455785; ENSG00000174943.
GeneID	253980.
KEGG	hsa:253980.
UCSC	uc002duv.3. human.
Organism-specific databases
CTD	253980.
GeneCards	GC16M029917.
HGNC	HGNC:22234. KCTD13.
HPA	HPA043524.
MIM	608947. gene.
neXtProt	NX_Q8WZ19.
PharmGKB	PA134907908.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG257745.
HOGENOM	HOG000294088.
HOVERGEN	HBG052219.
InParanoid	Q8WZ19.
KO	K15074.
OMA	DEDNREH.
OrthoDB	EOG4MPHQM.
PhylomeDB	Q8WZ19.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q8WZ19.
Bgee	Q8WZ19.
CleanEx	HS_KCTD13.
Genevestigator	Q8WZ19.
GermOnline	ENSG00000174943. Homo sapiens.
Family and domain databases
Gene3D	3.30.710.10. 1 hit.
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR003131. T1-type_BTB. [Graphical view]
Pfam	PF02214. K_tetra. 1 hit. [Graphical view]
SMART	SM00225. BTB. 1 hit. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit.
PROSITE	PS50097. BTB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	253980.
NextBio	92216.
SOURCE	Search...

Entry information

Entry name

BACD1_HUMAN

Accession

Primary (citable) accession number: Q8WZ19
Secondary accession number(s): A8K0R5, Q96P93, Q96SA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.