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Protein

BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1

Gene

KCTD13

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • GTP-Rho binding Source: UniProtKB
  • identical protein binding Source: IntAct
  • protein domain specific binding Source: Ensembl

GO - Biological processi

  • cell migration Source: UniProtKB
  • DNA replication Source: UniProtKB
  • negative regulation of Rho protein signal transduction Source: UniProtKB
  • positive regulation of DNA replication Source: Ensembl
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
  • protein homooligomerization Source: InterPro
  • protein ubiquitination Source: UniProtKB
  • stress fiber assembly Source: UniProtKB

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1
Short name:
hBACURD1
Alternative name(s):
BTB/POZ domain-containing protein KCTD13
Polymerase delta-interacting protein 1
TNFAIP1-like protein
Gene namesi
Name:KCTD13
Synonyms:BACURD1, PDIP1, POLDIP1
ORF Names:FKSG86, PP6832
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000174943.9.
HGNCiHGNC:22234. KCTD13.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi84 – 86VLI → AAA: Abolishes interaction with CUL3 and induces abnormal actin stress fibers. 1 Publication3

Organism-specific databases

DisGeNETi253980.
OpenTargetsiENSG00000174943.
PharmGKBiPA134907908.

Polymorphism and mutation databases

BioMutaiKCTD13.
DMDMi51701604.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001912971 – 329BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1Add BLAST329

Proteomic databases

MaxQBiQ8WZ19.
PaxDbiQ8WZ19.
PeptideAtlasiQ8WZ19.
PRIDEiQ8WZ19.

PTM databases

iPTMnetiQ8WZ19.
PhosphoSitePlusiQ8WZ19.

Expressioni

Tissue specificityi

Expressed in a wide variety of tissues.1 Publication

Inductioni

By TNF and IL6/interleukin-6.1 Publication

Gene expression databases

BgeeiENSG00000174943.
CleanExiHS_KCTD13.
ExpressionAtlasiQ8WZ19. baseline and differential.
GenevisibleiQ8WZ19. HS.

Organism-specific databases

HPAiHPA043524.

Interactioni

Subunit structurei

Component of the BCR(BACURD1) E3 ubiquitin ligase complex, at least composed of CUL3, KCTD13/BACURD1 and RBX1. Interacts with RHOA; with a preference for RhoA-GDP. Interacts with POLD2 and PCNA.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • GTP-Rho binding Source: UniProtKB
  • identical protein binding Source: IntAct
  • protein domain specific binding Source: Ensembl

Protein-protein interaction databases

BioGridi129001. 30 interactors.
IntActiQ8WZ19. 20 interactors.
MINTiMINT-1458356.
STRINGi9606.ENSP00000311202.

Structurei

Secondary structure

1329
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi42 – 47Combined sources6
Beta strandi50 – 55Combined sources6
Helixi56 – 59Combined sources4
Beta strandi61 – 64Combined sources4
Helixi65 – 69Combined sources5
Turni70 – 72Combined sources3
Beta strandi84 – 86Combined sources3
Turni90 – 92Combined sources3
Helixi93 – 102Combined sources10
Helixi111 – 123Combined sources13
Helixi127 – 140Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4UIJX-ray2.70A/B/C/D27-144[»]
ProteinModelPortaliQ8WZ19.
SMRiQ8WZ19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 109BTBPROSITE-ProRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the BACURD family.Curated

Phylogenomic databases

eggNOGiKOG2716. Eukaryota.
ENOG410XQYZ. LUCA.
GeneTreeiENSGT00900000140932.
HOGENOMiHOG000294088.
HOVERGENiHBG052219.
InParanoidiQ8WZ19.
KOiK15074.
OMAiHIMHDER.
OrthoDBiEOG091G0L1Y.
PhylomeDBiQ8WZ19.
TreeFamiTF315649.

Family and domain databases

InterProiView protein in InterPro
IPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
PfamiView protein in Pfam
PF02214. BTB_2. 1 hit.
SMARTiView protein in SMART
SM00225. BTB. 1 hit.
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiView protein in PROSITE
PS50097. BTB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8WZ19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAEASGPAA AAAPSLEAPK PSGLEPGPAA YGLKPLTPNS KYVKLNVGGS
60 70 80 90 100
LHYTTLRTLT GQDTMLKAMF SGRVEVLTDA GGWVLIDRSG RHFGTILNYL
110 120 130 140 150
RDGSVPLPES TRELGELLGE ARYYLVQGLI EDCQLALQQK RETLSPLCLI
160 170 180 190 200
PMVTSPREEQ QLLASTSKPV VKLLHNRSNN KYSYTSTSDD NLLKNIELFD
210 220 230 240 250
KLALRFHGRL LFLKDVLGDE ICCWSFYGQG RKIAEVCCTS IVYATEKKQT
260 270 280 290 300
KVEFPEARIF EETLNILIYE TPRGPDPALL EATGGAAGAG GAGRGEDEEN
310 320
REHRVRRIHV RRHITHDERP HGQQIVFKD
Length:329
Mass (Da):36,357
Last modified:March 1, 2002 - v1
Checksum:i17A8AC2DBE81EE78
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti251K → N in AAK27301 (Ref. 3) Curated1
Sequence conflicti328K → R in AAK27301 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF401315 mRNA. Translation: AAL14962.2.
AY027918 mRNA. Translation: AAK27301.1.
AF289573 mRNA. Translation: AAL55757.1.
AK289630 mRNA. Translation: BAF82319.1.
AC120114 Genomic DNA. No translation available.
CH471238 Genomic DNA. Translation: EAW79969.1.
BC036228 mRNA. Translation: AAH36228.1.
CCDSiCCDS10661.1.
RefSeqiNP_849194.1. NM_178863.4.
UniGeneiHs.534590.
Hs.657408.

Genome annotation databases

EnsembliENST00000308768; ENSP00000311202; ENSG00000174943.
ENST00000568000; ENSP00000455785; ENSG00000174943.
GeneIDi253980.
KEGGihsa:253980.
UCSCiuc002duv.5. human.

Similar proteinsi

Entry informationi

Entry nameiBACD1_HUMAN
AccessioniPrimary (citable) accession number: Q8WZ19
Secondary accession number(s): A8K0R5, Q96P93, Q96SA1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: March 1, 2002
Last modified: October 25, 2017
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families