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Q8WYN0

- ATG4A_HUMAN

UniProt

Q8WYN0 - ATG4A_HUMAN

Protein

Cysteine protease ATG4A

Gene

ATG4A

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.6 Publications

    Enzyme regulationi

    Inhibited by N-ethylmaleimide. Redox-regulated during autophagy since reducing conditions activate ATG4A whereas an oxidizing environment such as the presence of H2O2 inhibits its activity.2 Publications

    Kineticsi

    1. KM=33.9 µM for MAP1LC3B2 Publications
    2. KM=20.8 µM for GABARAP2 Publications
    3. KM=36.7 µM for GABARAPL12 Publications
    4. KM=15.7 µM for GABARAPL22 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei77 – 771NucleophileBy similarity
    Active sitei279 – 2791Sequence Analysis
    Active sitei281 – 2811By similarity

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB

    GO - Biological processi

    1. autophagy Source: UniProtKB-KW
    2. protein transport Source: UniProtKB-KW
    3. proteolysis Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Autophagy, Protein transport, Transport, Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC54.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine protease ATG4A (EC:3.4.22.-)
    Alternative name(s):
    AUT-like 2 cysteine endopeptidase
    Autophagin-2
    Autophagy-related cysteine endopeptidase 2
    Autophagy-related protein 4 homolog A
    Short name:
    hAPG4A
    Gene namesi
    Name:ATG4A
    Synonyms:APG4A, AUTL2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome X

    Organism-specific databases

    HGNCiHGNC:16489. ATG4A.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi81 – 811C → A: Reduces the redox sensitivity and retains activity in presence of H(2)O(2). 1 Publication

    Organism-specific databases

    PharmGKBiPA25184.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 398398Cysteine protease ATG4APRO_0000215838Add
    BLAST

    Proteomic databases

    MaxQBiQ8WYN0.
    PaxDbiQ8WYN0.
    PRIDEiQ8WYN0.

    Expressioni

    Tissue specificityi

    Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.2 Publications

    Gene expression databases

    ArrayExpressiQ8WYN0.
    BgeeiQ8WYN0.
    GenevestigatoriQ8WYN0.

    Organism-specific databases

    HPAiHPA036374.

    Interactioni

    Protein-protein interaction databases

    BioGridi125418. 3 interactions.
    IntActiQ8WYN0. 2 interactions.
    MINTiMINT-3048289.

    Structurei

    Secondary structure

    1
    398
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi28 – 314
    Beta strandi34 – 374
    Turni38 – 414
    Helixi42 – 509
    Beta strandi57 – 615
    Turni64 – 674
    Turni73 – 753
    Helixi77 – 9418
    Helixi110 – 1167
    Beta strandi119 – 1213
    Helixi128 – 1369
    Turni137 – 1393
    Helixi148 – 15912
    Turni163 – 1653
    Beta strandi168 – 1714
    Beta strandi176 – 1783
    Helixi179 – 1868
    Beta strandi223 – 2308
    Beta strandi233 – 2353
    Helixi238 – 2403
    Helixi241 – 2499
    Beta strandi253 – 2608
    Beta strandi263 – 2719
    Beta strandi274 – 2785
    Beta strandi282 – 2854
    Helixi298 – 3003
    Beta strandi307 – 3104
    Helixi311 – 3133
    Beta strandi316 – 32611
    Helixi327 – 34014
    Turni341 – 3433
    Beta strandi344 – 3463
    Beta strandi348 – 3547

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FUYmodel-A1-398[»]
    2P82X-ray2.10A/B/C/D23-359[»]
    ProteinModelPortaliQ8WYN0.
    SMRiQ8WYN0. Positions 26-359.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8WYN0.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase C54 family.Curated

    Phylogenomic databases

    eggNOGiNOG239662.
    HOVERGENiHBG050536.
    InParanoidiQ8WYN0.
    KOiK08342.
    OMAiKGTSAYC.
    OrthoDBiEOG73V6KD.
    PhylomeDBiQ8WYN0.
    TreeFamiTF314847.

    Family and domain databases

    InterProiIPR005078. Peptidase_C54.
    [Graphical view]
    PANTHERiPTHR22624. PTHR22624. 1 hit.
    PfamiPF03416. Peptidase_C54. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8WYN0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MESVLSKYED QITIFTDYLE EYPDTDELVW ILGKQHLLKT EKSKLLSDIS    50
    ARLWFTYRRK FSPIGGTGPS SDAGWGCMLR CGQMMLAQAL ICRHLGRDWS 100
    WEKQKEQPKE YQRILQCFLD RKDCCYSIHQ MAQMGVGEGK SIGEWFGPNT 150
    VAQVLKKLAL FDEWNSLAVY VSMDNTVVIE DIKKMCRVLP LSADTAGDRP 200
    PDSLTASNQS KGTSAYCSAW KPLLLIVPLR LGINQINPVY VDAFKECFKM 250
    PQSLGALGGK PNNAYYFIGF LGDELIFLDP HTTQTFVDTE ENGTVNDQTF 300
    HCLQSPQRMN ILNLDPSVAL GFFCKEEKDF DNWCSLVQKE ILKENLRMFE 350
    LVQKHPSHWP PFVPPAKPEV TTTGAEFIDS TEQLEEFDLE EDFEILSV 398
    Length:398
    Mass (Da):45,378
    Last modified:March 1, 2002 - v1
    Checksum:i3BE892E22E432151
    GO
    Isoform 2 (identifier: Q8WYN0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         211-272: Missing.

    Show »
    Length:336
    Mass (Da):38,601
    Checksum:i61D7819B73A0D8A9
    GO
    Isoform 3 (identifier: Q8WYN0-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-77: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:321
    Mass (Da):36,533
    Checksum:i5F6CAD07A44EC130
    GO
    Isoform 4 (identifier: Q8WYN0-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         41-64: Missing.

    Note: Gene prediction based on EST data.

    Show »
    Length:374
    Mass (Da):42,453
    Checksum:i4BB385D3C84939CB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti49 – 491I → Y in AAH41862. (PubMed:15489334)Curated
    Sequence conflicti218 – 2181S → T in CAC69076. 1 PublicationCurated
    Sequence conflicti218 – 2181S → T in CAC69077. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 7777Missing in isoform 3. 1 PublicationVSP_025902Add
    BLAST
    Alternative sequencei41 – 6424Missing in isoform 4. CuratedVSP_030499Add
    BLAST
    Alternative sequencei211 – 27262Missing in isoform 2. 1 PublicationVSP_013025Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ504651 mRNA. Translation: CAD43218.1.
    AB066214 mRNA. Translation: BAB83889.1.
    AJ320508 mRNA. Translation: CAC69076.1.
    AJ320509 mRNA. Translation: CAC69077.1.
    AK314429 mRNA. Translation: BAG37044.1.
    AL031177 Genomic DNA. Translation: CAI43137.1.
    AL031177 Genomic DNA. Translation: CAI43138.1.
    AL031177 Genomic DNA. Translation: CAI43141.1.
    AL031177 Genomic DNA. Translation: CAI43142.1.
    CH471120 Genomic DNA. Translation: EAX02689.1.
    CH471120 Genomic DNA. Translation: EAX02691.1.
    CH471120 Genomic DNA. Translation: EAX02693.1.
    BC041862 mRNA. Translation: AAH41862.1.
    BC061696 mRNA. Translation: AAH61696.1.
    CCDSiCCDS14538.1. [Q8WYN0-1]
    CCDS14539.1. [Q8WYN0-2]
    RefSeqiNP_443168.2. NM_052936.3. [Q8WYN0-1]
    NP_840054.1. NM_178270.2. [Q8WYN0-2]
    XP_005262121.1. XM_005262064.1. [Q8WYN0-3]
    UniGeneiHs.8763.

    Genome annotation databases

    EnsembliENST00000345734; ENSP00000298131; ENSG00000101844. [Q8WYN0-2]
    ENST00000372232; ENSP00000361306; ENSG00000101844. [Q8WYN0-1]
    GeneIDi115201.
    KEGGihsa:115201.
    UCSCiuc004enr.3. human. [Q8WYN0-1]
    uc004ent.3. human. [Q8WYN0-2]

    Polymorphism databases

    DMDMi61211859.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ504651 mRNA. Translation: CAD43218.1 .
    AB066214 mRNA. Translation: BAB83889.1 .
    AJ320508 mRNA. Translation: CAC69076.1 .
    AJ320509 mRNA. Translation: CAC69077.1 .
    AK314429 mRNA. Translation: BAG37044.1 .
    AL031177 Genomic DNA. Translation: CAI43137.1 .
    AL031177 Genomic DNA. Translation: CAI43138.1 .
    AL031177 Genomic DNA. Translation: CAI43141.1 .
    AL031177 Genomic DNA. Translation: CAI43142.1 .
    CH471120 Genomic DNA. Translation: EAX02689.1 .
    CH471120 Genomic DNA. Translation: EAX02691.1 .
    CH471120 Genomic DNA. Translation: EAX02693.1 .
    BC041862 mRNA. Translation: AAH41862.1 .
    BC061696 mRNA. Translation: AAH61696.1 .
    CCDSi CCDS14538.1. [Q8WYN0-1 ]
    CCDS14539.1. [Q8WYN0-2 ]
    RefSeqi NP_443168.2. NM_052936.3. [Q8WYN0-1 ]
    NP_840054.1. NM_178270.2. [Q8WYN0-2 ]
    XP_005262121.1. XM_005262064.1. [Q8WYN0-3 ]
    UniGenei Hs.8763.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FUY model - A 1-398 [» ]
    2P82 X-ray 2.10 A/B/C/D 23-359 [» ]
    ProteinModelPortali Q8WYN0.
    SMRi Q8WYN0. Positions 26-359.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 125418. 3 interactions.
    IntActi Q8WYN0. 2 interactions.
    MINTi MINT-3048289.

    Protein family/group databases

    MEROPSi C54.002.

    Polymorphism databases

    DMDMi 61211859.

    Proteomic databases

    MaxQBi Q8WYN0.
    PaxDbi Q8WYN0.
    PRIDEi Q8WYN0.

    Protocols and materials databases

    DNASUi 115201.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000345734 ; ENSP00000298131 ; ENSG00000101844 . [Q8WYN0-2 ]
    ENST00000372232 ; ENSP00000361306 ; ENSG00000101844 . [Q8WYN0-1 ]
    GeneIDi 115201.
    KEGGi hsa:115201.
    UCSCi uc004enr.3. human. [Q8WYN0-1 ]
    uc004ent.3. human. [Q8WYN0-2 ]

    Organism-specific databases

    CTDi 115201.
    GeneCardsi GC0XP107334.
    HGNCi HGNC:16489. ATG4A.
    HPAi HPA036374.
    MIMi 300663. gene.
    neXtProti NX_Q8WYN0.
    PharmGKBi PA25184.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG239662.
    HOVERGENi HBG050536.
    InParanoidi Q8WYN0.
    KOi K08342.
    OMAi KGTSAYC.
    OrthoDBi EOG73V6KD.
    PhylomeDBi Q8WYN0.
    TreeFami TF314847.

    Miscellaneous databases

    EvolutionaryTracei Q8WYN0.
    GeneWikii ATG4A.
    GenomeRNAii 115201.
    NextBioi 79530.
    PROi Q8WYN0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8WYN0.
    Bgeei Q8WYN0.
    Genevestigatori Q8WYN0.

    Family and domain databases

    InterProi IPR005078. Peptidase_C54.
    [Graphical view ]
    PANTHERi PTHR22624. PTHR22624. 1 hit.
    Pfami PF03416. Peptidase_C54. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy."
      Marino G., Uria J.A., Puente X.S., Quesada V., Bordallo J., Lopez-Otin C.
      J. Biol. Chem. 278:3671-3678(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Tissue: Ovary.
    2. "LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation."
      Kabeya Y., Mizushima N., Yamamoto A., Oshitani-Okamoto S., Ohsumi Y., Yoshimori T.
      J. Cell Sci. 117:2805-2812(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION IN GABARAPL2; GABARAP AND MAP1LC3 CLEAVAGE, TISSUE SPECIFICITY.
    3. "Cloning and sequencing of a second human homologue of the yeast Apg4 cysteine endopeptidase involved in autophagy."
      Chen J.M., Barrett A.J.
      Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
      Tissue: Prostate and Testis.
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. "The DNA sequence of the human X chromosome."
      Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
      , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
      Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Tissue: Kidney and Testis.
    8. "The COOH terminus of GATE-16, an intra-Golgi transport modulator, is cleaved by the human cysteine protease HsApg4A."
      Scherz-Shouval R., Sagiv Y., Shorer H., Elazar Z.
      J. Biol. Chem. 278:14053-14058(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN GABARAPL2 CLEAVAGE, ENZYME REGULATION.
    9. "Reactive oxygen species are essential for autophagy and specifically regulate the activity of Atg4."
      Scherz-Shouval R., Shvets E., Fass E., Shorer H., Gil L., Elazar Z.
      EMBO J. 26:1749-1760(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION, MUTAGENESIS OF CYS-81.
    10. "Kinetics comparisons of mammalian Atg4 homologues indicate selective preferences toward diverse Atg8 substrates."
      Li M., Hou Y., Wang J., Chen X., Shao Z.M., Yin X.M.
      J. Biol. Chem. 286:7327-7338(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    11. "High-throughput fluorescence assay for small-molecule inhibitors of autophagins/Atg4."
      Shu C.W., Madiraju C., Zhai D., Welsh K., Diaz P., Sergienko E., Sano R., Reed J.C.
      J. Biomol. Screen. 16:174-182(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "A high-throughput FRET-based assay for determination of Atg4 activity."
      Li M., Chen X., Ye Q.Z., Vogt A., Yin X.M.
      Autophagy 8:401-412(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    13. "Human cysteine protease ATG4A."
      Walker J.R., Davis T., Mujib S., Butler-Cole C., Finerty P.J., Weigelt J., Sundstrom M., Arrowsmith C.H., Edwards A.M., Bochkarev A., Dhe-Paganon S.
      Submitted (MAR-2007) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 23-359.

    Entry informationi

    Entry nameiATG4A_HUMAN
    AccessioniPrimary (citable) accession number: Q8WYN0
    Secondary accession number(s): A6NCH2
    , B2RAZ7, D3DUY0, O95534, Q5JYY9, Q5JYZ0, Q86VE5, Q96KQ0, Q96KQ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome X
      Human chromosome X: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. Peptidase families
      Classification of peptidase families and list of entries
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3