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Protein

Protein phosphatase Slingshot homolog 1

Gene

SSH1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.10 Publications

Miscellaneous

Tyrosine phosphatase activity has not been demonstrated for this protein to date.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei393Phosphocysteine intermediateCurated1

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • phosphoprotein phosphatase activity Source: UniProtKB
  • protein tyrosine/serine/threonine phosphatase activity Source: InterPro
  • protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Hydrolase, Protein phosphatase

Enzyme and pathway databases

SIGNORiQ8WYL5

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase Slingshot homolog 1 (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
SSH-like protein 1
Short name:
SSH-1L
Short name:
hSSH-1L
Gene namesi
Name:SSH1
Synonyms:KIAA1298, SSH1L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000084112.14
HGNCiHGNC:30579 SSH1
MIMi606778 gene
neXtProtiNX_Q8WYL5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi393C → S: Abrogates phosphatase activity. 8 Publications1
Mutagenesisi458W → A: Impairs stimulation of phosphatase activity by actin but does not affect basal activity. 1 Publication1
Mutagenesisi937S → A: Reduces binding to YWHAB, YWHAG, YWHAQ and YWHAZ. Abolishes binding to YWHAB, YWHAG, YWHAQ and YWHAZ and increases association with F-actin; when associated with A-978. 1 Publication1
Mutagenesisi978S → A: Reduces binding to YWHAB, YWHAG, YWHAQ and YWHAZ. Abolishes binding to YWHAB, YWHAG, YWHAQ and YWHAZ and increases association with F-actin; when associated with A-937. 1 Publication1

Organism-specific databases

DisGeNETi54434
OpenTargetsiENSG00000084112
PharmGKBiPA134941788

Polymorphism and mutation databases

BioMutaiSSH1
DMDMi82582267

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000948412 – 1049Protein phosphatase Slingshot homolog 1Add BLAST1048

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei37PhosphoserineCombined sources1
Modified residuei57PhosphoserineCombined sources1
Modified residuei515PhosphoserineBy similarity1
Modified residuei576PhosphoserineCombined sources1
Modified residuei897PhosphoserineCombined sources1
Modified residuei978Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated. Inhibitory phosphorylation by PAK4 promotes binding to YWHAZ. Phosphorylation at Ser-978 is decreased by stimuli which promote actin reorganization and lamellipodia formation. Can be dephosphorylated and activated by PPP3CA/calcineurin A. Phosphorylation decreases immediately prior to telophase.4 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8WYL5
MaxQBiQ8WYL5
PaxDbiQ8WYL5
PeptideAtlasiQ8WYL5
PRIDEiQ8WYL5
ProteomicsDBi75168
75169 [Q8WYL5-2]
75170 [Q8WYL5-3]
75171 [Q8WYL5-4]
75172 [Q8WYL5-5]

PTM databases

DEPODiQ8WYL5
iPTMnetiQ8WYL5
PhosphoSitePlusiQ8WYL5

Expressioni

Gene expression databases

BgeeiENSG00000084112
CleanExiHS_SSH1
ExpressionAtlasiQ8WYL5 baseline and differential
GenevisibleiQ8WYL5 HS

Organism-specific databases

HPAiHPA019845

Interactioni

Subunit structurei

Interacts with actin and this stimulates phosphatase activity. Also interacts with LIMK1 and with the 14-3-3 proteins YWHAB, YWHAG, YWHAQ, and YWHAZ. Interaction with 14-3-3 proteins inhibits phosphatase activity and also blocks recruitment to lamellipodia and stimulation by actin.4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • actin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi119950, 37 interactors
IntActiQ8WYL5, 13 interactors
MINTiQ8WYL5
STRINGi9606.ENSP00000315713

Structurei

3D structure databases

ProteinModelPortaliQ8WYL5
SMRiQ8WYL5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini308 – 448Tyrosine-protein phosphataseAdd BLAST141

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni897 – 1049Interaction with YWHAG1 PublicationAdd BLAST153

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118902
HOGENOMiHOG000154427
HOVERGENiHBG094001
InParanoidiQ8WYL5
KOiK05766
OMAiNSHCDKN
OrthoDBiEOG091G0249
PhylomeDBiQ8WYL5
TreeFamiTF319444

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR014876 DEK_C
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR029021 Prot-tyrosine_phosphatase-like
IPR027233 SSH1
IPR016130 Tyr_Pase_AS
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PTHR10159:SF138 PTHR10159:SF138, 1 hit
PfamiView protein in Pfam
PF08766 DEK_C, 1 hit
PF00782 DSPc, 1 hit
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WYL5-1) [UniParc]FASTAAdd to basket
Also known as: L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALVTLQRSP TPSAASSSAS NSELEAGSEE DRKLNLSLSE SFFMVKGAAL
60 70 80 90 100
FLQQGSSPQG QRSLQHPHKH AGDLPQHLQV MINLLRCEDR IKLAVRLESA
110 120 130 140 150
WADRVRYMVV VYSSGRQDTE ENILLGVDFS SKESKSCTIG MVLRLWSDTK
160 170 180 190 200
IHLDGDGGFS VSTAGRMHIF KPVSVQAMWS ALQVLHKACE VARRHNYFPG
210 220 230 240 250
GVALIWATYY ESCISSEQSC INEWNAMQDL ESTRPDSPAL FVDKPTEGER
260 270 280 290 300
TERLIKAKLR SIMMSQDLEN VTSKEIRNEL EKQMNCNLKE LKEFIDNEML
310 320 330 340 350
LILGQMDKPS LIFDHLYLGS EWNASNLEEL QGSGVDYILN VTREIDNFFP
360 370 380 390 400
GLFAYHNIRV YDEETTDLLA HWNEAYHFIN KAKRNHSKCL VHCKMGVSRS
410 420 430 440 450
ASTVIAYAMK EFGWPLEKAY NYVKQKRSIT RPNAGFMRQL SEYEGILDAS
460 470 480 490 500
KQRHNKLWRQ QTDSSLQQPV DDPAGPGDFL PETPDGTPES QLPFLDDAAQ
510 520 530 540 550
PGLGPPLPCC FRRLSDPLLP SPEDETGSLV HLEDPEREAL LEEAAPPAEV
560 570 580 590 600
HRPARQPQQG SGLCEKDVKK KLEFGSPKGR SGSLLQVEET EREEGLGAGR
610 620 630 640 650
WGQLPTQLDQ NLLNSENLNN NSKRSCPNGM EDDAIFGILN KVKPSYKSCA
660 670 680 690 700
DCMYPTASGA PEASRERCED PNAPAICTQP AFLPHITSSP VAHLASRSRV
710 720 730 740 750
PEKPASGPTE PPPFLPPAGS RRADTSGPGA GAALEPPASL LEPSRETPKV
760 770 780 790 800
LPKSLLLKNS HCDKNPPSTE VVIKEESSPK KDMKPAKDLR LLFSNESEKP
810 820 830 840 850
TTNSYLMQHQ ESIIQLQKAG LVRKHTKELE RLKSVPADPA PPSRDGPASR
860 870 880 890 900
LEASIPEESQ DPAALHELGP LVMPSQAGSD EKSEAAPASL EGGSLKSPPP
910 920 930 940 950
FFYRLDHTSS FSKDFLKTIC YTPTSSSMSS NLTRSSSSDS IHSVRGKPGL
960 970 980 990 1000
VKQRTQEIET RLRLAGLTVS SPLKRSHSLA KLGSLTFSTE DLSSEADPST
1010 1020 1030 1040
VADSQDTTLS ESSFLHEPQG TPRDPAATSK PSGKPAPENL KSPSWMSKS
Length:1,049
Mass (Da):115,511
Last modified:November 22, 2005 - v2
Checksum:i0600B9690F0E6F9C
GO
Isoform 2 (identifier: Q8WYL5-2) [UniParc]FASTAAdd to basket
Also known as: S

The sequence of this isoform differs from the canonical sequence as follows:
     632-692: DDAIFGILNK...LPHITSSPVA → VGRARPAGWH...LQGPEGSFTG
     693-1049: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:692
Mass (Da):77,430
Checksum:i9DC1FF2FC8984384
GO
Isoform 3 (identifier: Q8WYL5-3) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.
     74-93: LPQHLQVMINLLRCEDRIKL → MGGRHHLQRQVSESMSALFQ
     135-157: Missing.
     245-1049: Missing.

Note: Due to intron retention. No experimental confirmation available.
Show »
Length:148
Mass (Da):16,695
Checksum:i3EF171D933E3424D
GO
Isoform 4 (identifier: Q8WYL5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-312: Missing.
     313-334: FDHLYLGSEWNASNLEELQGSG → MRCYLSWDRWTSPPLSSIIFIS

Show »
Length:737
Mass (Da):80,684
Checksum:iE3825E2328B088D2
GO
Isoform 5 (identifier: Q8WYL5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-37: MALVTLQRSP...SEEDRKLNLS → MARARRAVVG...CCPEVSSSNY
     632-692: DDAIFGILNK...LPHITSSPVA → VGRARPAGWH...LQGPEGSFTG
     693-1049: Missing.

Note: No experimental confirmation available.
Show »
Length:703
Mass (Da):78,978
Checksum:i3ACFAB4B97CBDF7A
GO

Sequence cautioni

The sequence BAA92536 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2A → V in AAH62341 (PubMed:15489334).Curated1
Sequence conflicti230L → P in BAB84116 (PubMed:11832213).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0163111 – 312Missing in isoform 4. 1 PublicationAdd BLAST312
Alternative sequenceiVSP_0163121 – 73Missing in isoform 3. 1 PublicationAdd BLAST73
Alternative sequenceiVSP_0163131 – 37MALVT…KLNLS → MARARRAVVGSVRDVSTAAT NLFYFTDFCIFLQPTHCFCC PEVSSSNY in isoform 5. 1 PublicationAdd BLAST37
Alternative sequenceiVSP_01631474 – 93LPQHL…DRIKL → MGGRHHLQRQVSESMSALFQ in isoform 3. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_016315135 – 157Missing in isoform 3. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_016316245 – 1049Missing in isoform 3. 1 PublicationAdd BLAST805
Alternative sequenceiVSP_016317313 – 334FDHLY…LQGSG → MRCYLSWDRWTSPPLSSIIF IS in isoform 4. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_016318632 – 692DDAIF…SSPVA → VGRARPAGWHTPSLPSHSNW PTSASVVGTTGTRHHTQLIF FYCLLWAPSSHLQGPEGSFT G in isoform 2 and isoform 5. 2 PublicationsAdd BLAST61
Alternative sequenceiVSP_016319693 – 1049Missing in isoform 2 and isoform 5. 2 PublicationsAdd BLAST357

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB072355 mRNA Translation: BAB84114.1
AB072356 mRNA Translation: BAB84115.1
AB072357 mRNA Translation: BAB84116.1
AB037719 mRNA Translation: BAA92536.1 Different initiation.
AK095421 mRNA Translation: BAC04546.1
BC062341 mRNA Translation: AAH62341.1
CCDSiCCDS53825.1 [Q8WYL5-5]
CCDS55882.1 [Q8WYL5-2]
CCDS9121.1 [Q8WYL5-1]
RefSeqiNP_001154802.1, NM_001161330.1 [Q8WYL5-2]
NP_001154803.1, NM_001161331.1 [Q8WYL5-5]
NP_061857.3, NM_018984.3 [Q8WYL5-1]
XP_016874982.1, XM_017019493.1 [Q8WYL5-4]
XP_016874983.1, XM_017019494.1 [Q8WYL5-4]
UniGeneiHs.199763

Genome annotation databases

EnsembliENST00000326470; ENSP00000326107; ENSG00000084112 [Q8WYL5-5]
ENST00000326495; ENSP00000315713; ENSG00000084112 [Q8WYL5-1]
ENST00000551165; ENSP00000448824; ENSG00000084112 [Q8WYL5-2]
GeneIDi54434
KEGGihsa:54434
UCSCiuc001tnm.4 human [Q8WYL5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSSH1_HUMAN
AccessioniPrimary (citable) accession number: Q8WYL5
Secondary accession number(s): Q6P6C0
, Q8N9A7, Q8WYL3, Q8WYL4, Q9P2P8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: June 20, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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