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Q8WYK0

- ACO12_HUMAN

UniProt

Q8WYK0 - ACO12_HUMAN

Protein

Acyl-coenzyme A thioesterase 12

Gene

ACOT12

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Hydrolyzes acetyl-CoA to acetate and CoA.1 Publication

    Catalytic activityi

    Acetyl-CoA + H2O = CoA + acetate.

    Enzyme regulationi

    Inhibited by ADP. Active in the presence of ATP.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei144 – 1441Coenzyme A1 Publication

    GO - Molecular functioni

    1. acetyl-CoA hydrolase activity Source: HGNC
    2. ATP binding Source: Ensembl
    3. lipid binding Source: InterPro

    GO - Biological processi

    1. acetyl-CoA metabolic process Source: Ensembl
    2. acyl-CoA metabolic process Source: HGNC
    3. fatty acid metabolic process Source: UniProtKB-KW
    4. pyruvate metabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Enzyme and pathway databases

    BRENDAi3.1.2.1. 2681.
    SABIO-RKQ8WYK0.
    UniPathwayiUPA00231.

    Protein family/group databases

    TCDBi4.C.3.1.2. the acyl-coa thioesterase (acot) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-coenzyme A thioesterase 12 (EC:3.1.2.1)
    Short name:
    Acyl-CoA thioesterase 12
    Alternative name(s):
    Acyl-CoA thioester hydrolase 12
    Cytoplasmic acetyl-CoA hydrolase 1
    Short name:
    CACH-1
    Short name:
    hCACH-1
    START domain-containing protein 15
    Short name:
    StARD15
    Gene namesi
    Name:ACOT12
    Synonyms:CACH, CACH1, STARD15
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:24436. ACOT12.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosol Source: HGNC

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142672657.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 555555Acyl-coenzyme A thioesterase 12PRO_0000053809Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei33 – 331N6-succinyllysineBy similarity
    Modified residuei159 – 1591N6-succinyllysineBy similarity
    Modified residuei228 – 2281N6-succinyllysineBy similarity

    Proteomic databases

    PaxDbiQ8WYK0.
    PeptideAtlasiQ8WYK0.
    PRIDEiQ8WYK0.

    PTM databases

    PhosphoSiteiQ8WYK0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8WYK0.
    BgeeiQ8WYK0.
    CleanExiHS_ACOT12.
    GenevestigatoriQ8WYK0.

    Organism-specific databases

    HPAiHPA037723.
    HPA037724.

    Interactioni

    Subunit structurei

    Homodimer or homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi9606.ENSP00000303246.

    Structurei

    Secondary structure

    1
    555
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 148
    Helixi17 – 193
    Beta strandi24 – 263
    Helixi28 – 4720
    Beta strandi51 – 566
    Beta strandi69 – 8012
    Beta strandi82 – 9514
    Turni96 – 983
    Beta strandi101 – 11313
    Helixi131 – 14919
    Beta strandi183 – 1886
    Helixi191 – 1933
    Helixi202 – 21817
    Beta strandi220 – 2234
    Beta strandi226 – 2305
    Beta strandi244 – 25512
    Beta strandi258 – 26811
    Helixi270 – 2756
    Beta strandi279 – 28911
    Helixi294 – 2963

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3B7KX-ray2.70A/B/C7-316[»]
    ProteinModelPortaliQ8WYK0.
    SMRiQ8WYK0. Positions 7-543.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8WYK0.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 127127Acyl coenzyme A hydrolase 1Add
    BLAST
    Domaini165 – 301137Acyl coenzyme A hydrolase 2Add
    BLAST
    Domaini340 – 549210STARTPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni53 – 553Coenzyme A binding
    Regioni82 – 843Coenzyme A binding
    Regioni234 – 2363Coenzyme A binding

    Sequence similaritiesi

    Contains 1 START domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1607.
    HOGENOMiHOG000232032.
    HOVERGENiHBG023847.
    InParanoidiQ8WYK0.
    KOiK01067.
    OMAiPLWDPHY.
    OrthoDBiEOG70CR6C.
    PhylomeDBiQ8WYK0.
    TreeFamiTF328368.

    Family and domain databases

    Gene3Di3.10.129.10. 2 hits.
    3.30.530.20. 1 hit.
    InterProiIPR029069. HotDog_dom.
    IPR023393. START-like_dom.
    IPR002913. START_lipid-bd_dom.
    IPR006683. Thioestr_supf.
    [Graphical view]
    PfamiPF03061. 4HBT. 2 hits.
    PF01852. START. 1 hit.
    [Graphical view]
    SUPFAMiSSF54637. SSF54637. 2 hits.
    PROSITEiPS50848. START. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8WYK0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MERPAPGEVV MSQAIQPAHA TARGELSAGQ LLKWIDTTAC LAAEKHAGVS    50
    CVTASVDDIQ FEETARVGQV ITIKAKVTRA FSTSMEISIK VMVQDMLTGI 100
    EKLVSVAFST FVAKPVGKEK IHLKPVTLLT EQDHVEHNLA AERRKVRLQH 150
    EDTFNNLMKE SSKFDDLIFD EEEGAVSTRG TSVQSIELVL PPHANHHGNT 200
    FGGQIMAWME TVATISASRL CWAHPFLKSV DMFKFRGPST VGDRLVFTAI 250
    VNNTFQTCVE VGVRVEAFDC QEWAEGRGRH INSAFLIYNA ADDKENLITF 300
    PRIQPISKDD FRRYRGAIAR KRIRLGRKYV ISHKEEVPLC IHWDISKQAS 350
    LSDSNVEALK KLAAKRGWEV TSTVEKIKIY TLEEHDVLSV WVEKHVGSPA 400
    HLAYRLLSDF TKRPLWDPHF VSCEVIDWVS EDDQLYHITC PILNDDKPKD 450
    LVVLVSRRKP LKDGNTYTVA VKSVILPSVP PSPQYIRSEI ICAGFLIHAI 500
    DSNSCIVSYF NHMSASILPY FAGNLGGWSK SIEETAASCI QFLENPPDDG 550
    FVSTF 555
    Length:555
    Mass (Da):62,034
    Last modified:March 1, 2002 - v1
    Checksum:i707560D55504732C
    GO
    Isoform 2 (identifier: Q8WYK0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         166-167: DL → GQ
         168-555: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:167
    Mass (Da):18,327
    Checksum:i58CB03F09579E5A4
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti190 – 1901L → H Found in a clear cell renal carcinoma case; somatic mutation. 1 Publication
    VAR_064691
    Natural varianti230 – 2301V → I.
    Corresponds to variant rs34607174 [ dbSNP | Ensembl ].
    VAR_048192
    Natural varianti403 – 4031A → T.
    Corresponds to variant rs10371 [ dbSNP | Ensembl ].
    VAR_048193

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei166 – 1672DL → GQ in isoform 2. 1 PublicationVSP_055785
    Alternative sequencei168 – 555388Missing in isoform 2. 1 PublicationVSP_055786Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB078619 mRNA. Translation: BAB84022.1.
    AK122960 mRNA. Translation: BAG53821.1.
    AC008411 Genomic DNA. No translation available.
    AC010623 Genomic DNA. No translation available.
    CH471084 Genomic DNA. Translation: EAW95875.1.
    BC089437 mRNA. Translation: AAH89437.1.
    BC075010 mRNA. Translation: AAH75010.1.
    BC075011 mRNA. Translation: AAH75011.1.
    CCDSiCCDS4055.1.
    RefSeqiNP_570123.1. NM_130767.2.
    UniGeneiHs.591756.

    Genome annotation databases

    EnsembliENST00000307624; ENSP00000303246; ENSG00000172497. [Q8WYK0-1]
    ENST00000513751; ENSP00000421628; ENSG00000172497. [Q8WYK0-2]
    GeneIDi134526.
    KEGGihsa:134526.
    UCSCiuc003khl.4. human.

    Polymorphism databases

    DMDMi25008183.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB078619 mRNA. Translation: BAB84022.1 .
    AK122960 mRNA. Translation: BAG53821.1 .
    AC008411 Genomic DNA. No translation available.
    AC010623 Genomic DNA. No translation available.
    CH471084 Genomic DNA. Translation: EAW95875.1 .
    BC089437 mRNA. Translation: AAH89437.1 .
    BC075010 mRNA. Translation: AAH75010.1 .
    BC075011 mRNA. Translation: AAH75011.1 .
    CCDSi CCDS4055.1.
    RefSeqi NP_570123.1. NM_130767.2.
    UniGenei Hs.591756.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3B7K X-ray 2.70 A/B/C 7-316 [» ]
    ProteinModelPortali Q8WYK0.
    SMRi Q8WYK0. Positions 7-543.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000303246.

    Protein family/group databases

    TCDBi 4.C.3.1.2. the acyl-coa thioesterase (acot) family.

    PTM databases

    PhosphoSitei Q8WYK0.

    Polymorphism databases

    DMDMi 25008183.

    Proteomic databases

    PaxDbi Q8WYK0.
    PeptideAtlasi Q8WYK0.
    PRIDEi Q8WYK0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000307624 ; ENSP00000303246 ; ENSG00000172497 . [Q8WYK0-1 ]
    ENST00000513751 ; ENSP00000421628 ; ENSG00000172497 . [Q8WYK0-2 ]
    GeneIDi 134526.
    KEGGi hsa:134526.
    UCSCi uc003khl.4. human.

    Organism-specific databases

    CTDi 134526.
    GeneCardsi GC05M080662.
    HGNCi HGNC:24436. ACOT12.
    HPAi HPA037723.
    HPA037724.
    MIMi 614315. gene.
    neXtProti NX_Q8WYK0.
    PharmGKBi PA142672657.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1607.
    HOGENOMi HOG000232032.
    HOVERGENi HBG023847.
    InParanoidi Q8WYK0.
    KOi K01067.
    OMAi PLWDPHY.
    OrthoDBi EOG70CR6C.
    PhylomeDBi Q8WYK0.
    TreeFami TF328368.

    Enzyme and pathway databases

    UniPathwayi UPA00231 .
    BRENDAi 3.1.2.1. 2681.
    SABIO-RK Q8WYK0.

    Miscellaneous databases

    EvolutionaryTracei Q8WYK0.
    GeneWikii ACOT12.
    GenomeRNAii 134526.
    NextBioi 83397.
    PROi Q8WYK0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8WYK0.
    Bgeei Q8WYK0.
    CleanExi HS_ACOT12.
    Genevestigatori Q8WYK0.

    Family and domain databases

    Gene3Di 3.10.129.10. 2 hits.
    3.30.530.20. 1 hit.
    InterProi IPR029069. HotDog_dom.
    IPR023393. START-like_dom.
    IPR002913. START_lipid-bd_dom.
    IPR006683. Thioestr_supf.
    [Graphical view ]
    Pfami PF03061. 4HBT. 2 hits.
    PF01852. START. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54637. SSF54637. 2 hits.
    PROSITEi PS50848. START. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and functional expression of human cytosolic acetyl-CoA hydrolase."
      Suematsu N., Isohashi F.
      Acta Biochim. Pol. 53:553-561(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, ENZYME REGULATION, FUNCTION.
      Tissue: Liver.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Liver.
    3. "The DNA sequence and comparative analysis of human chromosome 5."
      Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
      , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
      Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Chondrosarcoma.
    6. "Human acyl-coenzyme A thioesterase 12."
      Structural genomics consortium (SGC)
      Submitted (NOV-2007) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 7-316 IN COMPLEX WITH COENZYME A.
    7. Cited for: VARIANT HIS-190.

    Entry informationi

    Entry nameiACO12_HUMAN
    AccessioniPrimary (citable) accession number: Q8WYK0
    Secondary accession number(s): B3KVK9, Q5FWE9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2002
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3