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Protein

Acyl-coenzyme A thioesterase 12

Gene

ACOT12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes acetyl-CoA to acetate and CoA.1 Publication

Catalytic activityi

Acetyl-CoA + H2O = CoA + acetate.

Enzyme regulationi

Inhibited by ADP. Active in the presence of ATP.1 Publication

Pathwayi: pyruvate metabolism

This protein is involved in the pathway pyruvate metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway pyruvate metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei144Coenzyme A1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

BioCyciZFISH:HS10529-MONOMER.
BRENDAi3.1.2.1. 2681.
ReactomeiR-HSA-75105. Fatty Acyl-CoA Biosynthesis.
UniPathwayiUPA00231.

Protein family/group databases

TCDBi4.C.3.1.2. the acyl-coa thioesterase (acot) family.

Chemistry databases

SwissLipidsiSLP:000001187.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A thioesterase 12 (EC:3.1.2.1)
Short name:
Acyl-CoA thioesterase 12
Alternative name(s):
Acyl-CoA thioester hydrolase 12
Cytoplasmic acetyl-CoA hydrolase 1
Short name:
CACH-1
Short name:
hCACH-1
START domain-containing protein 15
Short name:
StARD15
Gene namesi
Name:ACOT12
Synonyms:CACH, CACH1, STARD15
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:24436. ACOT12.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi134526.
OpenTargetsiENSG00000172497.
PharmGKBiPA142672657.

Polymorphism and mutation databases

BioMutaiACOT12.
DMDMi25008183.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538091 – 555Acyl-coenzyme A thioesterase 12Add BLAST555

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33N6-succinyllysineBy similarity1
Modified residuei159N6-succinyllysineBy similarity1
Modified residuei228N6-succinyllysineBy similarity1

Proteomic databases

PaxDbiQ8WYK0.
PeptideAtlasiQ8WYK0.
PRIDEiQ8WYK0.

PTM databases

iPTMnetiQ8WYK0.
PhosphoSitePlusiQ8WYK0.

Expressioni

Gene expression databases

BgeeiENSG00000172497.
CleanExiHS_ACOT12.
GenevisibleiQ8WYK0. HS.

Organism-specific databases

HPAiHPA037723.
HPA037724.

Interactioni

Subunit structurei

Homodimer or homotetramer.By similarity

Protein-protein interaction databases

BioGridi126405. 6 interactors.
IntActiQ8WYK0. 6 interactors.
STRINGi9606.ENSP00000303246.

Structurei

Secondary structure

1555
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 14Combined sources6
Helixi17 – 19Combined sources3
Beta strandi24 – 26Combined sources3
Helixi28 – 47Combined sources20
Beta strandi51 – 56Combined sources6
Beta strandi69 – 80Combined sources12
Beta strandi82 – 95Combined sources14
Turni96 – 98Combined sources3
Beta strandi101 – 113Combined sources13
Beta strandi116 – 118Combined sources3
Helixi131 – 160Combined sources30
Helixi171 – 173Combined sources3
Helixi179 – 181Combined sources3
Beta strandi183 – 188Combined sources6
Helixi191 – 193Combined sources3
Beta strandi198 – 200Combined sources3
Helixi202 – 221Combined sources20
Beta strandi222 – 230Combined sources9
Beta strandi244 – 255Combined sources12
Beta strandi258 – 268Combined sources11
Helixi270 – 274Combined sources5
Beta strandi279 – 291Combined sources13
Beta strandi293 – 295Combined sources3
Helixi308 – 325Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B7KX-ray2.70A/B/C7-316[»]
4MOBX-ray2.40A7-336[»]
4MOCX-ray2.50A7-336[»]
ProteinModelPortaliQ8WYK0.
SMRiQ8WYK0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8WYK0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 117HotDog ACOT-type 1PROSITE-ProRule annotationAdd BLAST113
Domaini179 – 294HotDog ACOT-type 2PROSITE-ProRule annotationAdd BLAST116
Domaini340 – 549STARTPROSITE-ProRule annotationAdd BLAST210

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni53 – 55Coenzyme A binding3
Regioni82 – 84Coenzyme A binding3
Regioni234 – 236Coenzyme A binding3

Sequence similaritiesi

Contains 2 HotDog ACOT-type domains.PROSITE-ProRule annotation
Contains 1 START domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2763. Eukaryota.
COG1607. LUCA.
GeneTreeiENSGT00760000119297.
HOGENOMiHOG000294223.
HOVERGENiHBG023847.
InParanoidiQ8WYK0.
KOiK01067.
OMAiPLWDPHY.
OrthoDBiEOG091G06LZ.
PhylomeDBiQ8WYK0.
TreeFamiTF328368.

Family and domain databases

Gene3Di3.10.129.10. 2 hits.
3.30.530.20. 1 hit.
InterProiIPR033120. HOTDOG_ACOT.
IPR029069. HotDog_dom.
IPR023393. START-like_dom.
IPR002913. START_lipid-bd_dom.
IPR006683. Thioestr_dom.
[Graphical view]
PfamiPF03061. 4HBT. 2 hits.
PF01852. START. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 2 hits.
PROSITEiPS51770. HOTDOG_ACOT. 2 hits.
PS50848. START. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WYK0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERPAPGEVV MSQAIQPAHA TARGELSAGQ LLKWIDTTAC LAAEKHAGVS
60 70 80 90 100
CVTASVDDIQ FEETARVGQV ITIKAKVTRA FSTSMEISIK VMVQDMLTGI
110 120 130 140 150
EKLVSVAFST FVAKPVGKEK IHLKPVTLLT EQDHVEHNLA AERRKVRLQH
160 170 180 190 200
EDTFNNLMKE SSKFDDLIFD EEEGAVSTRG TSVQSIELVL PPHANHHGNT
210 220 230 240 250
FGGQIMAWME TVATISASRL CWAHPFLKSV DMFKFRGPST VGDRLVFTAI
260 270 280 290 300
VNNTFQTCVE VGVRVEAFDC QEWAEGRGRH INSAFLIYNA ADDKENLITF
310 320 330 340 350
PRIQPISKDD FRRYRGAIAR KRIRLGRKYV ISHKEEVPLC IHWDISKQAS
360 370 380 390 400
LSDSNVEALK KLAAKRGWEV TSTVEKIKIY TLEEHDVLSV WVEKHVGSPA
410 420 430 440 450
HLAYRLLSDF TKRPLWDPHF VSCEVIDWVS EDDQLYHITC PILNDDKPKD
460 470 480 490 500
LVVLVSRRKP LKDGNTYTVA VKSVILPSVP PSPQYIRSEI ICAGFLIHAI
510 520 530 540 550
DSNSCIVSYF NHMSASILPY FAGNLGGWSK SIEETAASCI QFLENPPDDG

FVSTF
Length:555
Mass (Da):62,034
Last modified:March 1, 2002 - v1
Checksum:i707560D55504732C
GO
Isoform 2 (identifier: Q8WYK0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     166-167: DL → GQ
     168-555: Missing.

Note: No experimental confirmation available.
Show »
Length:167
Mass (Da):18,327
Checksum:i58CB03F09579E5A4
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_064691190L → H Found in a clear cell renal carcinoma case; somatic mutation. 1 Publication1
Natural variantiVAR_048192230V → I.Corresponds to variant rs34607174dbSNPEnsembl.1
Natural variantiVAR_048193403A → T.Corresponds to variant rs10371dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055785166 – 167DL → GQ in isoform 2. 1 Publication2
Alternative sequenceiVSP_055786168 – 555Missing in isoform 2. 1 PublicationAdd BLAST388

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB078619 mRNA. Translation: BAB84022.1.
AK122960 mRNA. Translation: BAG53821.1.
AC008411 Genomic DNA. No translation available.
AC010623 Genomic DNA. No translation available.
CH471084 Genomic DNA. Translation: EAW95875.1.
BC089437 mRNA. Translation: AAH89437.1.
BC075010 mRNA. Translation: AAH75010.1.
BC075011 mRNA. Translation: AAH75011.1.
CCDSiCCDS4055.1. [Q8WYK0-1]
RefSeqiNP_570123.1. NM_130767.2. [Q8WYK0-1]
UniGeneiHs.591756.

Genome annotation databases

EnsembliENST00000307624; ENSP00000303246; ENSG00000172497. [Q8WYK0-1]
ENST00000513751; ENSP00000421628; ENSG00000172497. [Q8WYK0-2]
GeneIDi134526.
KEGGihsa:134526.
UCSCiuc003khl.5. human. [Q8WYK0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB078619 mRNA. Translation: BAB84022.1.
AK122960 mRNA. Translation: BAG53821.1.
AC008411 Genomic DNA. No translation available.
AC010623 Genomic DNA. No translation available.
CH471084 Genomic DNA. Translation: EAW95875.1.
BC089437 mRNA. Translation: AAH89437.1.
BC075010 mRNA. Translation: AAH75010.1.
BC075011 mRNA. Translation: AAH75011.1.
CCDSiCCDS4055.1. [Q8WYK0-1]
RefSeqiNP_570123.1. NM_130767.2. [Q8WYK0-1]
UniGeneiHs.591756.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B7KX-ray2.70A/B/C7-316[»]
4MOBX-ray2.40A7-336[»]
4MOCX-ray2.50A7-336[»]
ProteinModelPortaliQ8WYK0.
SMRiQ8WYK0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126405. 6 interactors.
IntActiQ8WYK0. 6 interactors.
STRINGi9606.ENSP00000303246.

Chemistry databases

SwissLipidsiSLP:000001187.

Protein family/group databases

TCDBi4.C.3.1.2. the acyl-coa thioesterase (acot) family.

PTM databases

iPTMnetiQ8WYK0.
PhosphoSitePlusiQ8WYK0.

Polymorphism and mutation databases

BioMutaiACOT12.
DMDMi25008183.

Proteomic databases

PaxDbiQ8WYK0.
PeptideAtlasiQ8WYK0.
PRIDEiQ8WYK0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000307624; ENSP00000303246; ENSG00000172497. [Q8WYK0-1]
ENST00000513751; ENSP00000421628; ENSG00000172497. [Q8WYK0-2]
GeneIDi134526.
KEGGihsa:134526.
UCSCiuc003khl.5. human. [Q8WYK0-1]

Organism-specific databases

CTDi134526.
DisGeNETi134526.
GeneCardsiACOT12.
HGNCiHGNC:24436. ACOT12.
HPAiHPA037723.
HPA037724.
MIMi614315. gene.
neXtProtiNX_Q8WYK0.
OpenTargetsiENSG00000172497.
PharmGKBiPA142672657.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2763. Eukaryota.
COG1607. LUCA.
GeneTreeiENSGT00760000119297.
HOGENOMiHOG000294223.
HOVERGENiHBG023847.
InParanoidiQ8WYK0.
KOiK01067.
OMAiPLWDPHY.
OrthoDBiEOG091G06LZ.
PhylomeDBiQ8WYK0.
TreeFamiTF328368.

Enzyme and pathway databases

UniPathwayiUPA00231.
BioCyciZFISH:HS10529-MONOMER.
BRENDAi3.1.2.1. 2681.
ReactomeiR-HSA-75105. Fatty Acyl-CoA Biosynthesis.

Miscellaneous databases

EvolutionaryTraceiQ8WYK0.
GeneWikiiACOT12.
GenomeRNAii134526.
PROiQ8WYK0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000172497.
CleanExiHS_ACOT12.
GenevisibleiQ8WYK0. HS.

Family and domain databases

Gene3Di3.10.129.10. 2 hits.
3.30.530.20. 1 hit.
InterProiIPR033120. HOTDOG_ACOT.
IPR029069. HotDog_dom.
IPR023393. START-like_dom.
IPR002913. START_lipid-bd_dom.
IPR006683. Thioestr_dom.
[Graphical view]
PfamiPF03061. 4HBT. 2 hits.
PF01852. START. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 2 hits.
PROSITEiPS51770. HOTDOG_ACOT. 2 hits.
PS50848. START. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACO12_HUMAN
AccessioniPrimary (citable) accession number: Q8WYK0
Secondary accession number(s): B3KVK9, Q5FWE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.