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Q8WYJ6 (SEPT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Septin-1
Alternative name(s):
LARP
Peanut-like protein 3
Serologically defined breast cancer antigen NY-BR-24
Gene names
Name:SEPT1
Synonyms:DIFF6, PNUTL3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Filament-forming cytoskeletal GTPase By similarity. May play a role in cytokinesis Potential.

Subunit structure

Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation By similarity. Interacts with AURKB. Ref.7

Subcellular location

Cytoplasm By similarity. Cytoplasmcytoskeleton By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Midbody. Note: Remains at the centrosomes and the nearby microtubules throughout mitosis. Localizes to the midbody during cytokinesis. Ref.7 Ref.9

Tissue specificity

Expressed at high levels in lymphoid and hematopoietic tissues. Ref.8

Sequence similarities

Belongs to the septin family.

Sequence caution

The sequence AAK61491.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processCell cycle
Cell division
   Cellular componentCytoplasm
Cytoskeleton
   Coding sequence diversityAlternative splicing
Polymorphism
   LigandGTP-binding
Nucleotide-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

cell division

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentmicrotubule organizing center

Inferred from electronic annotation. Source: UniProtKB-SubCell

midbody

Inferred from electronic annotation. Source: UniProtKB-SubCell

septin complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

AURKBQ96GD46EBI-693002,EBI-624291

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8WYJ6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8WYJ6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     148-167: LRPLDVAFLRAVHEKVNIIP → SGGGILGAGAFREGWGVSAP
     168-367: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 367367Septin-1
PRO_0000173513

Regions

Nucleotide binding32 – 398GTP By similarity
Nucleotide binding171 – 1799GTP By similarity

Sites

Binding site661GTP By similarity
Binding site921GTP; via amide nitrogen By similarity
Binding site2291GTP; via amide nitrogen and carbonyl oxygen By similarity
Binding site2451GTP By similarity

Amino acid modifications

Modified residue2481Phosphoserine; by AURKB Ref.7
Modified residue3071Phosphoserine; by AURKB Ref.7
Modified residue3151Phosphoserine; by AURKB Ref.7

Natural variations

Alternative sequence148 – 16720LRPLD…VNIIP → SGGGILGAGAFREGWGVSAP in isoform 2.
VSP_038269
Alternative sequence168 – 367200Missing in isoform 2.
VSP_038270
Natural variant801G → V.
Corresponds to variant rs34518080 [ dbSNP | Ensembl ].
VAR_051934

Experimental info

Mutagenesis191S → A: No effect on phosphorylation. Ref.7
Mutagenesis2061S → A: No effect on phosphorylation. Ref.7
Mutagenesis2481S → A: Great reduction in phosphorylation. Ref.7
Mutagenesis3071S → A: Great reduction in phosphorylation. Ref.7
Mutagenesis3121S → A: No effect on phosphorylation. Ref.7
Mutagenesis3151S → A: Great reduction in phosphorylation. Ref.7
Sequence conflict601S → G in AAL40393. Ref.1
Sequence conflict148 – 1503LRP → SR in AAL40393. Ref.1
Sequence conflict240 – 2412RP → GL in AAL40393. Ref.1
Sequence conflict3241L → P in AAL40393. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 16, 2002. Version 2.
Checksum: E9C6BEF373CB0FC4

FASTA36741,971
        10         20         30         40         50         60 
MDKEYVGFAA LPNQLHRKSV KKGFDFTLMV AGESGLGKST LINSLFLTNL YEDRQVPEAS 

        70         80         90        100        110        120 
ARLTQTLAIE RRGVEIEEGG VKVKLTLVDT PGFGDSVDCS DCWLPVVKFI EEQFEQYLRD 

       130        140        150        160        170        180 
ESGLNRKNIQ DSRVHCCLYF ISPFGRGLRP LDVAFLRAVH EKVNIIPVIG KADALMPQET 

       190        200        210        220        230        240 
QALKQKIRDQ LKEEEIHIYQ FPECDSDEDE DFKRQDAEMK ESIPFAVVGS CEVVRDGGNR 

       250        260        270        280        290        300 
PVRGRRYSWG TVEVENPHHC DFLNLRRMLV QTHLQDLKEV THDLLYEGYR ARCLQSLARP 

       310        320        330        340        350        360 
GARDRASRSK LSRQSATEIP LPMLPLADTE KLIREKDEEL RRMQEMLEKM QAQMQQSQAQ 


GEQSDAL 

« Hide

Isoform 2 [UniParc].

Checksum: 2C7A515F1236AAC4
Show »

FASTA16718,354

References

« Hide 'large scale' references
[1]"Isolation and characterization of a novel human gene, HSLARP."
Tu Q., Yu L., Bi A., Zhang H., Zhao Y., Zhao S.
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Molecular cloning and characterization of human septins which are expressed in liver."
Peng J., Yu L.
Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Spleen.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: B-cell.
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 79-367.
Tissue: Stomach.
[6]"Humoral immunity to human breast cancer: antigen definition and quantitative analysis of mRNA expression."
Scanlan M.J., Gout I., Gordon C.M., Williamson B., Stockert E., Gure A.O., Jaeger D., Chen Y.-T., Mackay A., O'Hare M.J., Old L.J.
Cancer Immun. 1:4-4(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 93-367.
Tissue: Mammary gland.
[7]"Septin1, a new interaction partner for human serine/threonine kinase aurora-B."
Qi M., Yu W., Liu S., Jia H., Tang L., Shen M., Yan X., Saiyin H., Lang Q., Wan B., Zhao S., Yu L.
Biochem. Biophys. Res. Commun. 336:994-1000(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH AURKB, PHOSPHORYLATION AT SER-248; SER-307 AND SER-315, MUTAGENESIS OF SER-19; SER-206; SER-248; SER-307; SER-312 AND SER-315.
[8]"Expression profiling the human septin gene family."
Hall P.A., Jung K., Hillan K.J., Russell S.E.H.
J. Pathol. 206:269-278(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[9]"From midbody protein-protein interaction network construction to novel regulators in cytokinesis."
Chen T.C., Lee S.A., Hong T.M., Shih J.Y., Lai J.M., Chiou H.Y., Yang S.C., Chan C.H., Kao C.Y., Yang P.C., Huang C.Y.
J. Proteome Res. 8:4943-4953(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF085235 mRNA. Translation: AAL40393.1.
AY034176 mRNA. Translation: AAK61491.1. Different initiation.
AK301045 mRNA. Translation: BAG62658.1.
BC012161 mRNA. Translation: AAH12161.1.
AL833004 mRNA. Translation: CAH56484.1.
AF308288 Genomic DNA. Translation: AAG48256.1.
RefSeqNP_443070.5. NM_052838.4.
UniGeneHs.632176.

3D structure databases

ProteinModelPortalQ8WYJ6.
SMRQ8WYJ6. Positions 22-295.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8WYJ6. 5 interactions.
MINTMINT-1435466.
STRING9606.ENSP00000324511.

PTM databases

PhosphoSiteQ8WYJ6.

Polymorphism databases

DMDM20178107.

Proteomic databases

PaxDbQ8WYJ6.
PRIDEQ8WYJ6.

Protocols and materials databases

DNASU1731.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000571393; ENSP00000460441; ENSG00000180096. [Q8WYJ6-1]
GeneID1731.
KEGGhsa:1731.

Organism-specific databases

CTD1731.
GeneCardsGC16M030389.
HGNCHGNC:2879. SEPT1.
HPAHPA041566.
MIM612897. gene.
neXtProtNX_Q8WYJ6.
PharmGKBPA24354.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5019.
HOGENOMHOG000233586.
HOVERGENHBG065093.
InParanoidQ8WYJ6.
KOK13737.
PhylomeDBQ8WYJ6.
TreeFamTF101079.

Gene expression databases

BgeeQ8WYJ6.
CleanExHS_SEPT1.
GenevestigatorQ8WYJ6.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR000038. Cell_div_GTP-bd.
IPR027417. P-loop_NTPase.
IPR016491. Septin.
[Graphical view]
PANTHERPTHR18884. PTHR18884. 1 hit.
PfamPF00735. Septin. 1 hit.
[Graphical view]
PIRSFPIRSF006698. Septin. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Other

ChiTaRSSEPT1. human.
GeneWikiSEPT1.
GenomeRNAi1731.
NextBio7009.
PROQ8WYJ6.
SOURCESearch...

Entry information

Entry nameSEPT1_HUMAN
AccessionPrimary (citable) accession number: Q8WYJ6
Secondary accession number(s): B4DVE6 expand/collapse secondary AC list , Q658T1, Q8NEZ1, Q96EL4, Q9H285
Entry history
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: April 16, 2002
Last modified: April 16, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM