Q8WYJ6 (SEPT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Septin-1

Alternative name(s):
LARP
Peanut-like protein 3
Serologically defined breast cancer antigen NY-BR-24

Gene names

Name:	SEPT1
Synonyms:	DIFF6, PNUTL3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Filament-forming cytoskeletal GTPase By similarity. May play a role in cytokinesis Potential.
Subunit structure	Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation By similarity. Interacts with AURKB. Ref.7
Subcellular location	Cytoplasm By similarity. Cytoplasm › cytoskeleton By similarity. Cytoplasm › cytoskeleton › centrosome. Note: Remains at the centrosomes and the nearby microtubules throughout mitosis. Localizes to the midbody during cytokinesis. Ref.7
Tissue specificity	Expressed at high levels in lymphoid and hematopoietic tissues. Ref.8
Sequence similarities	Belongs to the septin family.
Sequence caution	The sequence AAK61491.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	GTP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell septin complex Inferred from electronic annotation. Source: InterPro
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AURKB	Q96GD4	6	EBI-693002,EBI-624291

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8WYJ6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8WYJ6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     148-167: LRPLDVAFLRAVHEKVNIIP → SGGGILGAGAFREGWGVSAP
     168-367: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Septin-1		PRO_0000173513
Regions
	Nucleotide binding	32 – 39	8	GTP By similarity
	Nucleotide binding	171 – 179	9	GTP By similarity
Sites
	Binding site	66	1	GTP By similarity
	Binding site	92	1	GTP; via amide nitrogen By similarity
	Binding site	229	1	GTP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	245	1	GTP By similarity
Amino acid modifications
	Modified residue	248	1	Phosphoserine; by AURKB Ref.7 Ref.9
	Modified residue	307	1	Phosphoserine; by AURKB Ref.7
	Modified residue	315	1	Phosphoserine; by AURKB Ref.7 Ref.9
Natural variations
	Alternative sequence	148 – 167	20	LRPLD…VNIIP → SGGGILGAGAFREGWGVSAP in isoform 2.		VSP_038269
	Alternative sequence	168 – 367	200	Missing in isoform 2.		VSP_038270
	Natural variant	80	1	G → V. Corresponds to variant rs34518080 [ dbSNP | Ensembl ].		VAR_051934
Experimental info
	Mutagenesis	19	1	S → A: No effect on phosphorylation. Ref.7
	Mutagenesis	206	1	S → A: No effect on phosphorylation. Ref.7
	Mutagenesis	248	1	S → A: Great reduction in phosphorylation. Ref.7
	Mutagenesis	307	1	S → A: Great reduction in phosphorylation. Ref.7
	Mutagenesis	312	1	S → A: No effect on phosphorylation. Ref.7
	Mutagenesis	315	1	S → A: Great reduction in phosphorylation. Ref.7
	Sequence conflict	60	1	S → G in AAL40393. Ref.1
	Sequence conflict	148 – 150	3	LRP → SR in AAL40393. Ref.1
	Sequence conflict	240 – 241	2	RP → GL in AAL40393. Ref.1
	Sequence conflict	324	1	L → P in AAL40393. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 16, 2002. Version 2. Checksum: E9C6BEF373CB0FC4	FASTA	367	41,971
	10 20 30 40 50 60 MDKEYVGFAA LPNQLHRKSV KKGFDFTLMV AGESGLGKST LINSLFLTNL YEDRQVPEAS 70 80 90 100 110 120 ARLTQTLAIE RRGVEIEEGG VKVKLTLVDT PGFGDSVDCS DCWLPVVKFI EEQFEQYLRD 130 140 150 160 170 180 ESGLNRKNIQ DSRVHCCLYF ISPFGRGLRP LDVAFLRAVH EKVNIIPVIG KADALMPQET 190 200 210 220 230 240 QALKQKIRDQ LKEEEIHIYQ FPECDSDEDE DFKRQDAEMK ESIPFAVVGS CEVVRDGGNR 250 260 270 280 290 300 PVRGRRYSWG TVEVENPHHC DFLNLRRMLV QTHLQDLKEV THDLLYEGYR ARCLQSLARP 310 320 330 340 350 360 GARDRASRSK LSRQSATEIP LPMLPLADTE KLIREKDEEL RRMQEMLEKM QAQMQQSQAQ GEQSDAL
	Isoform 2 [UniParc]. Checksum: 2C7A515F1236AAC4	FASTA	167	18,354
	10 20 30 40 50 60 MDKEYVGFAA LPNQLHRKSV KKGFDFTLMV AGESGLGKST LINSLFLTNL YEDRQVPEAS 70 80 90 100 110 120 ARLTQTLAIE RRGVEIEEGG VKVKLTLVDT PGFGDSVDCS DCWLPVVKFI EEQFEQYLRD 130 140 150 160 ESGLNRKNIQ DSRVHCCLYF ISPFGRGSGG GILGAGAFRE GWGVSAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a novel human gene, HSLARP." Tu Q., Yu L., Bi A., Zhang H., Zhao Y., Zhao S. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Molecular cloning and characterization of human septins which are expressed in liver." Peng J., Yu L. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Spleen.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: B-cell.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 79-367. Tissue: Stomach.
[6]	"Humoral immunity to human breast cancer: antigen definition and quantitative analysis of mRNA expression." Scanlan M.J., Gout I., Gordon C.M., Williamson B., Stockert E., Gure A.O., Jaeger D., Chen Y.-T., Mackay A., O'Hare M.J., Old L.J. Cancer Immun. 1:4-4(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 93-367. Tissue: Mammary gland.
[7]	"Septin1, a new interaction partner for human serine/threonine kinase aurora-B." Qi M., Yu W., Liu S., Jia H., Tang L., Shen M., Yan X., Saiyin H., Lang Q., Wan B., Zhao S., Yu L. Biochem. Biophys. Res. Commun. 336:994-1000(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH AURKB, PHOSPHORYLATION AT SER-248; SER-307 AND SER-315, MUTAGENESIS OF SER-19; SER-206; SER-248; SER-307; SER-312 AND SER-315.
[8]	"Expression profiling the human septin gene family." Hall P.A., Jung K., Hillan K.J., Russell S.E.H. J. Pathol. 206:269-278(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-248 AND SER-315, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF085235 mRNA. Translation: AAL40393.1. AY034176 mRNA. Translation: AAK61491.1. Different initiation. AK301045 mRNA. Translation: BAG62658.1. BC012161 mRNA. Translation: AAH12161.1. AL833004 mRNA. Translation: CAH56484.1. AF308288 Genomic DNA. Translation: AAG48256.1.
IPI	IPI00790521. IPI00910257.
RefSeq	NP_443070.5. NM_052838.4.
UniGene	Hs.632176.
3D structure databases
ProteinModelPortal	Q8WYJ6.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8WYJ6. 5 interactions.
MINT	MINT-1435466.
STRING	9606.ENSP00000324511.
PTM databases
PhosphoSite	Q8WYJ6.
Polymorphism databases
DMDM	20178107.
Proteomic databases
PaxDb	Q8WYJ6.
PRIDE	Q8WYJ6.
Protocols and materials databases
DNASU	1731.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000571393; ENSP00000460441; ENSG00000180096.
GeneID	1731.
KEGG	hsa:1731.
UCSC	uc002dxy.3. human.
Organism-specific databases
CTD	1731.
GeneCards	GC16M030389.
HGNC	HGNC:2879. SEPT1.
HPA	HPA041566.
MIM	612897. gene.
neXtProt	NX_Q8WYJ6.
PharmGKB	PA24354.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5019.
HOGENOM	HOG000233586.
HOVERGEN	HBG065093.
InParanoid	Q8WYJ6.
KO	K13737.
OMA	EEIHIYQ.
OrthoDB	EOG447FTF.
Enzyme and pathway databases
Pathway_Interaction_DB	aurora_b_pathway. Aurora B signaling.
Gene expression databases
ArrayExpress	Q8WYJ6.
Bgee	Q8WYJ6.
CleanEx	HS_SEPT1.
Genevestigator	Q8WYJ6.
GermOnline	ENSG00000180096. Homo sapiens.
Family and domain databases
InterPro	IPR000038. Cell_div_GTP-bd. IPR027417. P-loop_NTPase. IPR016491. Septin. [Graphical view]
PANTHER	PTHR18884. PTHR18884. 1 hit.
Pfam	PF00735. Septin. 1 hit. [Graphical view]
PIRSF	PIRSF006698. Septin. 1 hit.
SUPFAM	SSF52540. SSF52540. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	SEPT1. human.
GenomeRNAi	1731.
NextBio	7009.
SOURCE	Search...

Entry information

Entry name

SEPT1_HUMAN

Accession

Primary (citable) accession number: Q8WYJ6
Secondary accession number(s): B4DVE6 , Q658T1, Q8NEZ1, Q96EL4, Q9H285

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	April 16, 2002
Last modified:	May 29, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.