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Protein

Protein phosphatase 1E

Gene

PPM1E

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2 (By similarity). Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Dephosphorylates PRKAA2 (By similarity).By similarity1 Publication

Caution

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi273Manganese 1By similarity1
Metal bindingi273Manganese 2By similarity1
Metal bindingi274Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi435Manganese 2By similarity1
Metal bindingi479Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: UniProtKB

GO - Biological processi

  • cellular response to drug Source: UniProtKB
  • negative regulation of protein kinase activity Source: UniProtKB
  • peptidyl-threonine dephosphorylation Source: UniProtKB
  • positive regulation of stress fiber assembly Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

SIGNORiQ8WY54

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1E (EC:3.1.3.16)
Alternative name(s):
Ca(2+)/calmodulin-dependent protein kinase phosphatase N
Short name:
CaMKP-N
CaMKP-nucleus
Short name:
CaMKN
Partner of PIX 1
Partner of PIX-alpha
Short name:
Partner of PIXA
Gene namesi
Name:PPM1E
Synonyms:CAMKN, KIAA1072, POPX1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000175175.5
HGNCiHGNC:19322 PPM1E
neXtProtiNX_Q8WY54

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi22843
OpenTargetsiENSG00000175175
PharmGKBiPA134943567

Polymorphism and mutation databases

DMDMi311033412

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002868201 – 755Protein phosphatase 1EAdd BLAST755

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei535PhosphoserineBy similarity1
Modified residuei548PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8WY54
PaxDbiQ8WY54
PeptideAtlasiQ8WY54
PRIDEiQ8WY54

PTM databases

DEPODiQ8WY54
iPTMnetiQ8WY54
PhosphoSitePlusiQ8WY54

Expressioni

Gene expression databases

BgeeiENSG00000175175
CleanExiHS_PPM1E
GenevisibleiQ8WY54 HS

Organism-specific databases

HPAiHPA018462
HPA019263

Interactioni

Subunit structurei

Heterotrimer. Interacts with PAX1 and ARHGEF6 (or ARHGEF7).1 Publication

Protein-protein interaction databases

BioGridi11651529 interactors.
IntActiQ8WY54 2 interactors.
MINTiQ8WY54
STRINGi9606.ENSP00000312411

Structurei

3D structure databases

ProteinModelPortaliQ8WY54
SMRiQ8WY54
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati31 – 3212
Repeati33 – 3422
Repeati35 – 3632
Repeati37 – 3842
Repeati39 – 4052
Repeati41 – 4262
Repeati43 – 4472
Repeati45 – 468; approximate2
Repeati47 – 4892
Repeati49 – 50102
Repeati51 – 52112
Domaini231 – 488PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST258

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni31 – 5211 X 2 AA tandem repeats of P-EAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi7 – 100Glu-richAdd BLAST94

Sequence similaritiesi

Belongs to the PP2C family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0698 Eukaryota
COG0631 LUCA
GeneTreeiENSGT00900000140907
HOGENOMiHOG000059266
HOVERGENiHBG098260
InParanoidiQ8WY54
KOiK17501
OMAiPVEMFGP
OrthoDBiEOG091G08KG
PhylomeDBiQ8WY54
TreeFamiTF317617

Family and domain databases

CDDicd00143 PP2Cc, 1 hit
Gene3Di3.60.40.101 hit
InterProiView protein in InterPro
IPR015655 PP2C
IPR000222 PP2C_BS
IPR036457 PPM-type_dom_sf
IPR001932 PPM-type_phosphatase_dom
PANTHERiPTHR13832 PTHR13832, 1 hit
PfamiView protein in Pfam
PF00481 PP2C, 1 hit
SMARTiView protein in SMART
SM00332 PP2Cc, 1 hit
SUPFAMiSSF81606 SSF81606, 1 hit
PROSITEiView protein in PROSITE
PS01032 PPM_1, 1 hit
PS51746 PPM_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: Q8WY54-2) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGCIPEEKT YRRFLELFLG EFRGPCGGGE PEPEPEPEPE PEPESEPEPE
60 70 80 90 100
PELVEAEAAE ASVEEPGEEA ATVAATEEGD QEQDPEPEEE AAVEGEEEEE
110 120 130 140 150
GAATAAAAPG HSAVPPPPPQ LPPLPPLPRP LSERITREEV EGESLDLCLQ
160 170 180 190 200
QLYKYNCPSF LAAALARATS DEVLQSDLSA HYIPKETDGT EGTVEIETVK
210 220 230 240 250
LARSVFSKLH EICCSWVKDF PLRRRPQLYY ETSIHAIKNM RRKMEDKHVC
260 270 280 290 300
IPDFNMLFNL EDQEEQAYFA VFDGHGGVDA AIYASIHLHV NLVRQEMFPH
310 320 330 340 350
DPAEALCRAF RVTDERFVQK AARESLRCGT TGVVTFIRGN MLHVAWVGDS
360 370 380 390 400
QVMLVRKGQA VELMKPHKPD REDEKQRIEA LGGCVVWFGA WRVNGSLSVS
410 420 430 440 450
RAIGDAEHKP YICGDADSAS TVLDGTEDYL ILACDGFYDT VNPDEAVKVV
460 470 480 490 500
SDHLKENNGD SSMVAHKLVA SARDAGSSDN ITVIVVFLRD MNKAVNVSEE
510 520 530 540 550
SDWTENSFQG GQEDGGDDKE NHGECKRPWP QHQCSAPADL GYDGRVDSFT
560 570 580 590 600
DRTSLSPGSQ INVLEDPGYL DLTQIEASKP HSAQFLLPVE MFGPGAPKKA
610 620 630 640 650
NLINELMMEK KSVQSSLPEW SGAGEFPTAF NLGSTGEQIY RMQSLSPVCS
660 670 680 690 700
GLENEQFKSP GNRVSRLSHL RHHYSKKWHR FRFNPKFYSF LSAQEPSHKI
710 720 730 740 750
GTSLSSLTGS GKRNRIRSSL PWRQNSWKGY SENMRKLRKT HDIPCPDLPW

SYKIE
Length:755
Mass (Da):83,952
Last modified:March 28, 2018 - v3
Checksum:iC24C64A0A1BC1091
GO
Isoform 3 (identifier: Q8WY54-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-261: MAGCIPEEKT...PDFNMLFNLE → MKSFRVIFLHIISQRKRMAQKGLW

Note: No experimental confirmation available.
Show »
Length:518
Mass (Da):58,021
Checksum:i61334BB8591B377E
GO

Sequence cautioni

The sequence AAF70325 differs from that shown. Contains a 27 bp insertion which does not match the genome.Curated
The sequence BAA83024 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53L → P in DA497512 (PubMed:16344560).Curated1
Sequence conflicti385V → I in AAF70325 (Ref. 2) Curated1

Polymorphismi

The poly-Pro-Glu stretch is polymorphic.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06376944E → EPE7 Publications1
Natural variantiVAR_036518222L → S in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_036519311R → G in a breast cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0251981 – 261MAGCI…LFNLE → MKSFRVIFLHIISQRKRMAQ KGLW in isoform 3. 1 PublicationAdd BLAST261

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF520614 mRNA Translation: AAM76058.1
AF260269 mRNA Translation: AAF70325.1 Sequence problems.
AB028995 mRNA Translation: BAA83024.2 Different initiation.
AK289966 mRNA Translation: BAF82655.1
CR749253 mRNA Translation: CAH18109.1
AC025521 Genomic DNA No translation available.
AC100832 Genomic DNA No translation available.
CH471109 Genomic DNA Translation: EAW94429.1
BC136290 mRNA Translation: AAI36291.1
BC151228 mRNA Translation: AAI51229.1
DA497512 mRNA No translation available.
DA791319 mRNA No translation available.
CCDSiCCDS11613.1 [Q8WY54-2]
RefSeqiNP_055721.3, NM_014906.4 [Q8WY54-2]
XP_005257226.1, XM_005257169.4
XP_011522836.1, XM_011524534.2
UniGeneiHs.245044

Genome annotation databases

EnsembliENST00000308249; ENSP00000312411; ENSG00000175175 [Q8WY54-2]
GeneIDi22843
KEGGihsa:22843
UCSCiuc002iwx.5 human [Q8WY54-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPPM1E_HUMAN
AccessioniPrimary (citable) accession number: Q8WY54
Secondary accession number(s): A7E2X1
, Q68DW1, Q7LAF3, Q9UPT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: March 28, 2018
Last modified: April 25, 2018
This is version 127 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome