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Q8WY54

- PPM1E_HUMAN

UniProt

Q8WY54 - PPM1E_HUMAN

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Protein

Protein phosphatase 1E

Gene

PPM1E

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2 (By similarity). Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Dephosphorylates PRKAA2 (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi282 – 2821Manganese 1By similarity
Metal bindingi282 – 2821Manganese 2By similarity
Metal bindingi283 – 2831Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi444 – 4441Manganese 2By similarity
Metal bindingi488 – 4881Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: UniProtKB

GO - Biological processi

  1. cellular response to drug Source: UniProtKB
  2. negative regulation of protein kinase activity Source: UniProtKB
  3. peptidyl-threonine dephosphorylation Source: UniProtKB
  4. positive regulation of stress fiber assembly Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1E (EC:3.1.3.16)
Alternative name(s):
Ca(2+)/calmodulin-dependent protein kinase phosphatase N
Short name:
CaMKP-N
CaMKP-nucleus
Short name:
CaMKN
Partner of PIX 1
Partner of PIX-alpha
Short name:
Partner of PIXA
Gene namesi
Name:PPM1E
Synonyms:CAMKN, KIAA1072, POPX1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:19322. PPM1E.

Subcellular locationi

Nucleus. Cytoplasm
Note: A truncated form, major form, with the C-terminal part missing, is mostly found in the cytoplasm and a little in the nucleus. The full length, minor form, is found in the nucleus.

GO - Cellular componenti

  1. mitochondrion Source: Ensembl
  2. nucleolus Source: HPA
  3. nucleus Source: HPA
  4. protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134943567.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 764764Protein phosphatase 1EPRO_0000286820Add
BLAST

Proteomic databases

MaxQBiQ8WY54.
PaxDbiQ8WY54.
PRIDEiQ8WY54.

PTM databases

PhosphoSiteiQ8WY54.

Expressioni

Gene expression databases

BgeeiQ8WY54.
CleanExiHS_PPM1E.
ExpressionAtlasiQ8WY54. baseline and differential.
GenevestigatoriQ8WY54.

Organism-specific databases

HPAiHPA018462.
HPA019263.

Interactioni

Subunit structurei

Heterotrimer. Interacts with PAX1 and ARHGEF6 (or ARHGEF7).1 Publication

Protein-protein interaction databases

BioGridi116515. 5 interactions.
IntActiQ8WY54. 2 interactions.
MINTiMINT-2812827.
STRINGi9606.ENSP00000312411.

Structurei

3D structure databases

ProteinModelPortaliQ8WY54.
SMRiQ8WY54. Positions 245-498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati31 – 3221
Repeati33 – 3422
Repeati35 – 3623
Repeati37 – 3824
Repeati39 – 4025
Repeati41 – 4226
Repeati43 – 4427
Repeati45 – 4628; approximate
Repeati47 – 4829
Repeati49 – 50210
Repeati51 – 52211
Domaini255 – 497243PP2C-likeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni31 – 522211 X 2 AA tandem repeats of P-EAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi7 – 10094Glu-richAdd
BLAST
Compositional biasi31 – 139109Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PP2C-like domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000114971.
HOGENOMiHOG000059266.
HOVERGENiHBG098260.
InParanoidiQ8WY54.
KOiK17501.
OMAiPVEMFGP.
OrthoDBiEOG7D2FDH.
PhylomeDBiQ8WY54.
TreeFamiTF317617.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8WY54-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGCIPEEKT YRRFLELFLG EFRGPCGGGE PEPEPEPEPE PEPESEPEPE
60 70 80 90 100
PELVEAEAAE ASVEEPGEEA ATVAATEEGD QEQDPEPEEE AAVEGEEEEE
110 120 130 140 150
GAATAAAAPG HSAVPPPPPQ LPPLPPLPRP LSERITPRPL SERITREEVE
160 170 180 190 200
GESLDLCLQQ LYKYNCPSFL AAALARATSD EVLQSDLSAH YIPKETDGTE
210 220 230 240 250
GTVEIETVKL ARSVFSKLHE ICCSWVKDFP LRRRPQLYYE TSIHAIKNMR
260 270 280 290 300
RKMEDKHVCI PDFNMLFNLE DQEEQAYFAV FDGHGGVDAA IYASIHLHVN
310 320 330 340 350
LVRQEMFPHD PAEALCRAFR VTDERFVQKA ARESLRCGTT GVVTFIRGNM
360 370 380 390 400
LHVAWVGDSQ VMLVRKGQAV ELMKPHKPDR EDEKQRIEAL GGCVVWFGAW
410 420 430 440 450
RVNGSLSVSR AIGDAEHKPY ICGDADSAST VLDGTEDYLI LACDGFYDTV
460 470 480 490 500
NPDEAVKVVS DHLKENNGDS SMVAHKLVAS ARDAGSSDNI TVIVVFLRDM
510 520 530 540 550
NKAVNVSEES DWTENSFQGG QEDGGDDKEN HGECKRPWPQ HQCSAPADLG
560 570 580 590 600
YDGRVDSFTD RTSLSPGSQI NVLEDPGYLD LTQIEASKPH SAQFLLPVEM
610 620 630 640 650
FGPGAPKKAN LINELMMEKK SVQSSLPEWS GAGEFPTAFN LGSTGEQIYR
660 670 680 690 700
MQSLSPVCSG LENEQFKSPG NRVSRLSHLR HHYSKKWHRF RFNPKFYSFL
710 720 730 740 750
SAQEPSHKIG TSLSSLTGSG KRNRIRSSLP WRQNSWKGYS ENMRKLRKTH
760
DIPCPDLPWS YKIE
Length:764
Mass (Da):85,002
Last modified:November 2, 2010 - v2
Checksum:i16F79EB84ED0E295
GO
Isoform 2 (identifier: Q8WY54-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     133-141: Missing.

Show »
Length:755
Mass (Da):83,952
Checksum:iC24C64A0A1BC1091
GO
Isoform 3 (identifier: Q8WY54-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     247-270: KNMRRKMEDKHVCIPDFNMLFNLE → MKSFRVIFLHIISQRKRMAQKGLW

Note: No experimental confirmation available.

Show »
Length:764
Mass (Da):84,967
Checksum:i5B29325645AD2C48
GO

Sequence cautioni

The sequence BAA83024.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531L → P in DA497512. (PubMed:16344560)Curated
Sequence conflicti394 – 3941V → I in AAF70325. 1 PublicationCurated

Polymorphismi

The poly-Pro-Glu stretch is polymorphic.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti44 – 441E → EPE.3 Publications
VAR_063769
Natural varianti231 – 2311L → S in a breast cancer sample; somatic mutation. 1 Publication
VAR_036518
Natural varianti320 – 3201R → G in a breast cancer sample; somatic mutation. 1 Publication
VAR_036519

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei133 – 1419Missing in isoform 2. 2 PublicationsVSP_025197
Alternative sequencei247 – 27024KNMRR…LFNLE → MKSFRVIFLHIISQRKRMAQ KGLW in isoform 3. 1 PublicationVSP_025198Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF520614 mRNA. Translation: AAM76058.1.
AF260269 mRNA. Translation: AAF70325.1.
AB028995 mRNA. Translation: BAA83024.2. Different initiation.
CR749253 mRNA. Translation: CAH18109.1.
AC025521 Genomic DNA. No translation available.
AC100832 Genomic DNA. No translation available.
DA497512 mRNA. No translation available.
DA791319 mRNA. No translation available.
CCDSiCCDS11613.1. [Q8WY54-2]
RefSeqiXP_005257226.1. XM_005257169.2.
UniGeneiHs.245044.

Genome annotation databases

EnsembliENST00000308249; ENSP00000312411; ENSG00000175175. [Q8WY54-2]
GeneIDi22843.
KEGGihsa:22843.
UCSCiuc002iwx.4. human. [Q8WY54-2]
uc010ddd.4. human. [Q8WY54-3]

Polymorphism databases

DMDMi311033412.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF520614 mRNA. Translation: AAM76058.1 .
AF260269 mRNA. Translation: AAF70325.1 .
AB028995 mRNA. Translation: BAA83024.2 . Different initiation.
CR749253 mRNA. Translation: CAH18109.1 .
AC025521 Genomic DNA. No translation available.
AC100832 Genomic DNA. No translation available.
DA497512 mRNA. No translation available.
DA791319 mRNA. No translation available.
CCDSi CCDS11613.1. [Q8WY54-2 ]
RefSeqi XP_005257226.1. XM_005257169.2.
UniGenei Hs.245044.

3D structure databases

ProteinModelPortali Q8WY54.
SMRi Q8WY54. Positions 245-498.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116515. 5 interactions.
IntActi Q8WY54. 2 interactions.
MINTi MINT-2812827.
STRINGi 9606.ENSP00000312411.

PTM databases

PhosphoSitei Q8WY54.

Polymorphism databases

DMDMi 311033412.

Proteomic databases

MaxQBi Q8WY54.
PaxDbi Q8WY54.
PRIDEi Q8WY54.

Protocols and materials databases

DNASUi 22843.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000308249 ; ENSP00000312411 ; ENSG00000175175 . [Q8WY54-2 ]
GeneIDi 22843.
KEGGi hsa:22843.
UCSCi uc002iwx.4. human. [Q8WY54-2 ]
uc010ddd.4. human. [Q8WY54-3 ]

Organism-specific databases

CTDi 22843.
GeneCardsi GC17P056833.
H-InvDB HIX0014038.
HGNCi HGNC:19322. PPM1E.
HPAi HPA018462.
HPA019263.
neXtProti NX_Q8WY54.
PharmGKBi PA134943567.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0631.
GeneTreei ENSGT00740000114971.
HOGENOMi HOG000059266.
HOVERGENi HBG098260.
InParanoidi Q8WY54.
KOi K17501.
OMAi PVEMFGP.
OrthoDBi EOG7D2FDH.
PhylomeDBi Q8WY54.
TreeFami TF317617.

Miscellaneous databases

ChiTaRSi PPM1E. human.
GenomeRNAii 22843.
NextBioi 13643771.
PROi Q8WY54.

Gene expression databases

Bgeei Q8WY54.
CleanExi HS_PPM1E.
ExpressionAtlasi Q8WY54. baseline and differential.
Genevestigatori Q8WY54.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 1 hit.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family."
    Koh C.-G., Tan E.-J., Manser E., Lim L.
    Curr. Biol. 12:317-321(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, INTERACTION WITH ARHGEF, VARIANT PRO-GLU-44 INS.
  2. "Five novel genes from 17q23 amplicon have different amplification and overexpression frequency in breast cancer."
    Wu G., Couch F.J.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT PRO-GLU-44 INS.
  3. "Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT PRO-GLU-44 INS.
    Tissue: Brain.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Testis.
  5. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-112.
  7. "Identification and characterization of nuclear localization signals of CaMKP-N."
    Takeuchi M., Taniguchi T., Fujisawa H.
    J. Biochem. 136:183-188(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "Post-translational excision of the carboxyl-terminal segment of CaM kinase phosphatase N and its cytosolic occurrence in the brain."
    Kitani T., Okuno S., Nakamura Y., Tokuno H., Takeuchi M., Fujisawa H.
    J. Neurochem. 96:374-384(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. Cited for: VARIANTS [LARGE SCALE ANALYSIS] SER-231 AND GLY-320.

Entry informationi

Entry nameiPPM1E_HUMAN
AccessioniPrimary (citable) accession number: Q8WY54
Secondary accession number(s): Q68DW1, Q7LAF3, Q9UPT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: November 2, 2010
Last modified: November 26, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3