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Q8WY54 (PPM1E_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 1E

EC=3.1.3.16
Alternative name(s):
Ca(2+)/calmodulin-dependent protein kinase phosphatase N
Short name=CaMKP-N
CaMKP-nucleus
Short name=CaMKN
Partner of PIX 1
Partner of PIX-alpha
Short name=Partner of PIXA
Gene names
Name:PPM1E
Synonyms:CAMKN, KIAA1072, POPX1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length764 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2 By similarity. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Dephosphorylates PRKAA2 By similarity. Ref.1

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subunit structure

Heterotrimer. Interacts with PAX1 and ARHGEF6 (or ARHGEF7). Ref.1

Subcellular location

Nucleus. Cytoplasm. Note: A truncated form, major form, with the C-terminal part missing, is mostly found in the cytoplasm and a little in the nucleus. The full length, minor form, is found in the nucleus. Ref.7 Ref.8

Polymorphism

The poly-Pro-Glu stretch is polymorphic.

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Sequence caution

The sequence BAA83024.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8WY54-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8WY54-2)

The sequence of this isoform differs from the canonical sequence as follows:
     133-141: Missing.
Isoform 3 (identifier: Q8WY54-3)

The sequence of this isoform differs from the canonical sequence as follows:
     247-270: KNMRRKMEDKHVCIPDFNMLFNLE → MKSFRVIFLHIISQRKRMAQKGLW
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 764764Protein phosphatase 1E
PRO_0000286820

Regions

Repeat31 – 3221
Repeat33 – 3422
Repeat35 – 3623
Repeat37 – 3824
Repeat39 – 4025
Repeat41 – 4226
Repeat43 – 4427
Repeat45 – 4628; approximate
Repeat47 – 4829
Repeat49 – 50210
Repeat51 – 52211
Domain255 – 497243PP2C-like
Region31 – 522211 X 2 AA tandem repeats of P-E
Compositional bias7 – 10094Glu-rich
Compositional bias31 – 139109Pro-rich

Sites

Metal binding2821Manganese 1 By similarity
Metal binding2821Manganese 2 By similarity
Metal binding2831Manganese 1; via carbonyl oxygen By similarity
Metal binding4441Manganese 2 By similarity
Metal binding4881Manganese 2 By similarity

Natural variations

Alternative sequence133 – 1419Missing in isoform 2.
VSP_025197
Alternative sequence247 – 27024KNMRR…LFNLE → MKSFRVIFLHIISQRKRMAQ KGLW in isoform 3.
VSP_025198
Natural variant441E → EPE.
VAR_063769
Natural variant2311L → S in a breast cancer sample; somatic mutation. Ref.9
VAR_036518
Natural variant3201R → G in a breast cancer sample; somatic mutation. Ref.9
VAR_036519

Experimental info

Sequence conflict531L → P in DA497512. Ref.6
Sequence conflict3941V → I in AAF70325. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 2, 2010. Version 2.
Checksum: 16F79EB84ED0E295

FASTA76485,002
        10         20         30         40         50         60 
MAGCIPEEKT YRRFLELFLG EFRGPCGGGE PEPEPEPEPE PEPESEPEPE PELVEAEAAE 

        70         80         90        100        110        120 
ASVEEPGEEA ATVAATEEGD QEQDPEPEEE AAVEGEEEEE GAATAAAAPG HSAVPPPPPQ 

       130        140        150        160        170        180 
LPPLPPLPRP LSERITPRPL SERITREEVE GESLDLCLQQ LYKYNCPSFL AAALARATSD 

       190        200        210        220        230        240 
EVLQSDLSAH YIPKETDGTE GTVEIETVKL ARSVFSKLHE ICCSWVKDFP LRRRPQLYYE 

       250        260        270        280        290        300 
TSIHAIKNMR RKMEDKHVCI PDFNMLFNLE DQEEQAYFAV FDGHGGVDAA IYASIHLHVN 

       310        320        330        340        350        360 
LVRQEMFPHD PAEALCRAFR VTDERFVQKA ARESLRCGTT GVVTFIRGNM LHVAWVGDSQ 

       370        380        390        400        410        420 
VMLVRKGQAV ELMKPHKPDR EDEKQRIEAL GGCVVWFGAW RVNGSLSVSR AIGDAEHKPY 

       430        440        450        460        470        480 
ICGDADSAST VLDGTEDYLI LACDGFYDTV NPDEAVKVVS DHLKENNGDS SMVAHKLVAS 

       490        500        510        520        530        540 
ARDAGSSDNI TVIVVFLRDM NKAVNVSEES DWTENSFQGG QEDGGDDKEN HGECKRPWPQ 

       550        560        570        580        590        600 
HQCSAPADLG YDGRVDSFTD RTSLSPGSQI NVLEDPGYLD LTQIEASKPH SAQFLLPVEM 

       610        620        630        640        650        660 
FGPGAPKKAN LINELMMEKK SVQSSLPEWS GAGEFPTAFN LGSTGEQIYR MQSLSPVCSG 

       670        680        690        700        710        720 
LENEQFKSPG NRVSRLSHLR HHYSKKWHRF RFNPKFYSFL SAQEPSHKIG TSLSSLTGSG 

       730        740        750        760 
KRNRIRSSLP WRQNSWKGYS ENMRKLRKTH DIPCPDLPWS YKIE 

« Hide

Isoform 2 [UniParc].

Checksum: C24C64A0A1BC1091
Show »

FASTA75583,952
Isoform 3 [UniParc].

Checksum: 5B29325645AD2C48
Show »

FASTA76484,967

References

« Hide 'large scale' references
[1]"The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family."
Koh C.-G., Tan E.-J., Manser E., Lim L.
Curr. Biol. 12:317-321(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, INTERACTION WITH ARHGEF, VARIANT PRO-GLU-44 INS.
[2]"Five novel genes from 17q23 amplicon have different amplification and overexpression frequency in breast cancer."
Wu G., Couch F.J.
Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT PRO-GLU-44 INS.
[3]"Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT PRO-GLU-44 INS.
Tissue: Brain.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Testis.
[5]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes."
Kimura K., Wakamatsu A., Suzuki Y., Ota T., Nishikawa T., Yamashita R., Yamamoto J., Sekine M., Tsuritani K., Wakaguri H., Ishii S., Sugiyama T., Saito K., Isono Y., Irie R., Kushida N., Yoneyama T., Otsuka R. expand/collapse author list , Kanda K., Yokoi T., Kondo H., Wagatsuma M., Murakawa K., Ishida S., Ishibashi T., Takahashi-Fujii A., Tanase T., Nagai K., Kikuchi H., Nakai K., Isogai T., Sugano S.
Genome Res. 16:55-65(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-112.
[7]"Identification and characterization of nuclear localization signals of CaMKP-N."
Takeuchi M., Taniguchi T., Fujisawa H.
J. Biochem. 136:183-188(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[8]"Post-translational excision of the carboxyl-terminal segment of CaM kinase phosphatase N and its cytosolic occurrence in the brain."
Kitani T., Okuno S., Nakamura Y., Tokuno H., Takeuchi M., Fujisawa H.
J. Neurochem. 96:374-384(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[9]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] SER-231 AND GLY-320.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF520614 mRNA. Translation: AAM76058.1.
AF260269 mRNA. Translation: AAF70325.1.
AB028995 mRNA. Translation: BAA83024.2. Different initiation.
CR749253 mRNA. Translation: CAH18109.1.
AC025521 Genomic DNA. No translation available.
AC100832 Genomic DNA. No translation available.
DA497512 mRNA. No translation available.
DA791319 mRNA. No translation available.
RefSeqXP_005257226.1. XM_005257169.2.
UniGeneHs.245044.

3D structure databases

ProteinModelPortalQ8WY54.
SMRQ8WY54. Positions 245-498.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116515. 3 interactions.
IntActQ8WY54. 2 interactions.
MINTMINT-2812827.
STRING9606.ENSP00000312411.

PTM databases

PhosphoSiteQ8WY54.

Polymorphism databases

DMDM311033412.

Proteomic databases

PaxDbQ8WY54.
PRIDEQ8WY54.

Protocols and materials databases

DNASU22843.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000308249; ENSP00000312411; ENSG00000175175. [Q8WY54-2]
GeneID22843.
KEGGhsa:22843.
UCSCuc002iwx.4. human. [Q8WY54-2]
uc010ddd.4. human. [Q8WY54-3]

Organism-specific databases

CTD22843.
GeneCardsGC17P056833.
H-InvDBHIX0014038.
HGNCHGNC:19322. PPM1E.
HPAHPA018462.
HPA019263.
neXtProtNX_Q8WY54.
PharmGKBPA134943567.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0631.
HOGENOMHOG000059266.
HOVERGENHBG098260.
InParanoidQ8WY54.
KOK17501.
OMAPHSAQFL.
OrthoDBEOG7D2FDH.
PhylomeDBQ8WY54.
TreeFamTF317617.

Gene expression databases

ArrayExpressQ8WY54.
BgeeQ8WY54.
CleanExHS_PPM1E.
GenevestigatorQ8WY54.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 1 hit.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPPM1E. human.
GenomeRNAi22843.
NextBio13643771.
PROQ8WY54.

Entry information

Entry namePPM1E_HUMAN
AccessionPrimary (citable) accession number: Q8WY54
Secondary accession number(s): Q68DW1, Q7LAF3, Q9UPT0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: November 2, 2010
Last modified: April 16, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM