Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nanos homolog 1

Gene

NANOS1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri213 – 26755Nanos-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cell migration Source: UniProtKB
  • epithelial cell migration Source: UniProtKB
  • negative regulation of translation Source: BHF-UCL
  • positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: BHF-UCL
  • posttranscriptional regulation of gene expression Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Translation regulation

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Nanos homolog 1
Short name:
NOS-1
Alternative name(s):
EC_Rep1a
Gene namesi
Name:NANOS1
Synonyms:NOS1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:23044. NANOS1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Involvement in diseasei

Spermatogenic failure 12 (SPGF12)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn infertility disorder caused by spermatogenesis defects. It results in decreased sperm motility, concentration, and multiple sperm structural defects. Non-obstructive azoospermia, oligozoospermia and oligo-astheno-teratozoospermia are features observed in SPGF12 patients.
See also OMIM:615413
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti78 – 781Missing in SPGF12. 1 Publication
VAR_070570
Natural varianti173 – 1731Missing in SPGF12. 1 Publication
VAR_070571

Keywords - Diseasei

Disease mutation

Organism-specific databases

MalaCardsiNANOS1.
MIMi615413. phenotype.
Orphaneti399805. Male infertility with azoospermia or oligozoospermia due to single gene mutation.
399808. Male infertility with teratozoospermia due to single gene mutation.
PharmGKBiPA134876543.

Polymorphism and mutation databases

BioMutaiNANOS1.
DMDMi41688589.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292Nanos homolog 1PRO_0000207685Add
BLAST

Proteomic databases

PaxDbiQ8WY41.
PRIDEiQ8WY41.

PTM databases

iPTMnetiQ8WY41.
PhosphoSiteiQ8WY41.

Expressioni

Tissue specificityi

Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness.5 Publications

Developmental stagei

Fetal ovary and fetal testis (at protein level).1 Publication

Inductioni

Down-regulated by E-cadherin.1 Publication

Gene expression databases

BgeeiQ8WY41.
CleanExiHS_NANOS1.
HS_NOS1.
ExpressionAtlasiQ8WY41. baseline and differential.
GenevisibleiQ8WY41. HS.

Organism-specific databases

HPAiCAB012219.

Interactioni

Subunit structurei

Interacts with PUM2, SNAPIN and CTNNB1. Interacts (via N-terminal region) with CTNND1. Interacts with DDX20 (via N-terminal region).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CTNND1O60716-212EBI-9630165,EBI-9634525

Protein-protein interaction databases

BioGridi131092. 3 interactions.
IntActiQ8WY41. 1 interaction.
STRINGi9606.ENSP00000393275.

Structurei

Secondary structure

1
292
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi43 – 475Combined sources
Turni51 – 533Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4CQOX-ray2.80B/D40-56[»]
ProteinModelPortaliQ8WY41.
SMRiQ8WY41. Positions 214-267.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni40 – 5617Essential for its translational repressor activityBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi214 – 24128C2HC 1Add
BLAST
Motifi249 – 26517C2HC 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi136 – 20469Ala-richAdd
BLAST

Domaini

The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.PROSITE-ProRule annotation
The N-terminal region and C-terminal zinc-finger RNA-binding domain are both necessary for interaction with SNAPIN.

Sequence similaritiesi

Belongs to the nanos family.PROSITE-ProRule annotation
Contains 1 nanos-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri213 – 26755Nanos-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4602. Eukaryota.
ENOG41121KZ. LUCA.
GeneTreeiENSGT00640000091383.
HOGENOMiHOG000113729.
HOVERGENiHBG052574.
InParanoidiQ8WY41.
KOiK18741.
OMAiLCAGPAE.
PhylomeDBiQ8WY41.
TreeFamiTF326882.

Family and domain databases

InterProiIPR008705. Nanos/Xcar2.
IPR024161. Znf_nanos-typ.
[Graphical view]
PANTHERiPTHR12887. PTHR12887. 1 hit.
PfamiPF05741. zf-nanos. 1 hit.
[Graphical view]
PROSITEiPS51522. ZF_NANOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8WY41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAFPWAPRS PRRGRAPPPM ALVPSARYVS APGPAHPQPF SSWNDYLGLA
60 70 80 90 100
TLITKAVDGE PRFGCARGGN GGGGSPPSSS SSSCCSPHTG AGPGALGPAL
110 120 130 140 150
GPPDYDEDDD DDSDEPGSRG RYLGSALELR ALELCAGPAE AGLLEERFAE
160 170 180 190 200
LSPFAGRAAA VLLGCAPAAA AAATTTSEAT PREERAPAWA AEPRLHAASG
210 220 230 240 250
AAAARLLKPE LQVCVFCRNN KEAMALYTTH ILKGPDGRVL CPVLRRYTCP
260 270 280 290
LCGASGDNAH TIKYCPLSKV PPPPARPPPR SARDGPPGKK LR
Length:292
Mass (Da):30,230
Last modified:February 2, 2004 - v2
Checksum:i1E41AAE68992CDD7
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti34 – 341P → T.1 Publication
Corresponds to variant rs191267549 [ dbSNP | Ensembl ].
VAR_070569
Natural varianti78 – 781Missing in SPGF12. 1 Publication
VAR_070570
Natural varianti173 – 1731Missing in SPGF12. 1 Publication
VAR_070571
Natural varianti246 – 2461R → H Found in a patient affected by oligo-astheno-teratozoospermia also carrying Y-276 on the same allele; unknown pathological significance. 1 Publication
VAR_070572
Natural varianti276 – 2761R → Y Found in a patient affected by oligo-astheno-teratozoospermia also carrying H-246 on the same allele; requires 2 nucleotide substitutions; unknown pathological significance. 1 Publication
VAR_070573

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF275269 mRNA. Translation: AAL36982.2.
AF458985 mRNA. Translation: AAQ04765.1.
AL157788 Genomic DNA. Translation: CAI14718.1.
CCDSiCCDS7607.1.
RefSeqiNP_955631.1. NM_199461.3.
UniGeneiHs.591918.

Genome annotation databases

EnsembliENST00000425699; ENSP00000393275; ENSG00000188613.
GeneIDi340719.
KEGGihsa:340719.
UCSCiuc009xzf.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF275269 mRNA. Translation: AAL36982.2.
AF458985 mRNA. Translation: AAQ04765.1.
AL157788 Genomic DNA. Translation: CAI14718.1.
CCDSiCCDS7607.1.
RefSeqiNP_955631.1. NM_199461.3.
UniGeneiHs.591918.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4CQOX-ray2.80B/D40-56[»]
ProteinModelPortaliQ8WY41.
SMRiQ8WY41. Positions 214-267.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi131092. 3 interactions.
IntActiQ8WY41. 1 interaction.
STRINGi9606.ENSP00000393275.

PTM databases

iPTMnetiQ8WY41.
PhosphoSiteiQ8WY41.

Polymorphism and mutation databases

BioMutaiNANOS1.
DMDMi41688589.

Proteomic databases

PaxDbiQ8WY41.
PRIDEiQ8WY41.

Protocols and materials databases

DNASUi340719.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000425699; ENSP00000393275; ENSG00000188613.
GeneIDi340719.
KEGGihsa:340719.
UCSCiuc009xzf.2. human.

Organism-specific databases

CTDi340719.
GeneCardsiNANOS1.
HGNCiHGNC:23044. NANOS1.
HPAiCAB012219.
MalaCardsiNANOS1.
MIMi608226. gene.
615413. phenotype.
neXtProtiNX_Q8WY41.
Orphaneti399805. Male infertility with azoospermia or oligozoospermia due to single gene mutation.
399808. Male infertility with teratozoospermia due to single gene mutation.
PharmGKBiPA134876543.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4602. Eukaryota.
ENOG41121KZ. LUCA.
GeneTreeiENSGT00640000091383.
HOGENOMiHOG000113729.
HOVERGENiHBG052574.
InParanoidiQ8WY41.
KOiK18741.
OMAiLCAGPAE.
PhylomeDBiQ8WY41.
TreeFamiTF326882.

Miscellaneous databases

GenomeRNAii340719.
PROiQ8WY41.
SOURCEiSearch...

Gene expression databases

BgeeiQ8WY41.
CleanExiHS_NANOS1.
HS_NOS1.
ExpressionAtlasiQ8WY41. baseline and differential.
GenevisibleiQ8WY41. HS.

Family and domain databases

InterProiIPR008705. Nanos/Xcar2.
IPR024161. Znf_nanos-typ.
[Graphical view]
PANTHERiPTHR12887. PTHR12887. 1 hit.
PfamiPF05741. zf-nanos. 1 hit.
[Graphical view]
PROSITEiPS51522. ZF_NANOS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells."
    Jaruzelska J., Kotecki M., Kusz K., Spik A., Firpo M., Reijo Pera R.A.
    Dev. Genes Evol. 213:120-126(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH PUM2.
    Tissue: Testis.
  2. "E-cadherin regulates human Nanos1, which interacts with p120ctn and induces tumor cell migration and invasion."
    Strumane K., Bonnomet A., Stove C., Vandenbroucke R., Nawrocki-Raby B., Bruyneel E., Mareel M., Birembaut P., Berx G., van Roy F.
    Cancer Res. 66:10007-10015(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH CTNND1 AND CTNNB1, TISSUE SPECIFICITY.
  3. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The E-cadherin-repressed hNanos1 gene induces tumor cell invasion by upregulating MT1-MMP expression."
    Bonnomet A., Polette M., Strumane K., Gilles C., Dalstein V., Kileztky C., Berx G., van Roy F., Birembaut P., Nawrocki-Raby B.
    Oncogene 27:3692-3699(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  5. "The SNARE-associated component SNAPIN binds PUMILIO2 and NANOS1 proteins in human male germ cells."
    Ginter-Matuszewska B., Spik A., Rembiszewska A., Koyias C., Kupryjanczyk J., Jaruzelska J.
    Mol. Hum. Reprod. 15:173-179(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH PUM2 AND SNAPIN.
  6. "NANOS1 and PUMILIO2 bind microRNA biogenesis factor GEMIN3, within chromatoid body in human germ cells."
    Ginter-Matuszewska B., Kusz K., Spik A., Grzeszkowiak D., Rembiszewska A., Kupryjanczyk J., Jaruzelska J.
    Histochem. Cell Biol. 136:279-287(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DDX20.
  7. "NANOS3 function in human germ cell development."
    Julaton V.T., Reijo Pera R.A.
    Hum. Mol. Genet. 20:2238-2250(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  8. "Mutations of NANOS1, a human homologue of the Drosophila morphogen, are associated with a lack of germ cells in testes or severe oligo-astheno-teratozoospermia."
    Kusz-Zamelczyk K., Sajek M., Spik A., Glazar R., Jedrzejczak P., Latos-Bielenska A., Kotecki M., Pawelczyk L., Jaruzelska J.
    J. Med. Genet. 50:187-193(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS SPGF12 SER-78 DEL AND ALA-173 DEL, VARIANTS THR-34; HIS-246 AND TYR-276.

Entry informationi

Entry nameiNANO1_HUMAN
AccessioniPrimary (citable) accession number: Q8WY41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: June 8, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.