Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Progesterone-induced-blocking factor 1

Gene

PIBF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 1: Pericentriolar protein required to maintain mitotic spindle pole integrity (PubMed:21224392). Required for the centrosomal accumulation of PCM1 and the recruitment of centriolar satellite proteins such as BBS4. Via association with PCM1 may be involved in primary cilia formation (PubMed:23110211). Required for CEP63 centrosomal localization and its interaction with WDR62. Together with CEP63 promotes centriole duplication. Promotes the centrosomal localization of CDK2 (PubMed:26297806).3 Publications
Isoform 4: The secreted form is a mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling decidual naturakl killer cells (NK) activity exerts an anti-abortive effect (PubMed:14634107, PubMed:3863495, PubMed:12516630). Increases the production of Th2-type cytokines by signaling via the JAK/STAT pathway. Activates STAT6 and inhibits STAT4 phosphorylation. Signaling via a not identified receptor seems to implicate IL4R and a GPI-anchored protein (PubMed:16393965, PubMed:25218441).1 Publication5 Publications

GO - Molecular functioni

  • interleukin-4 receptor binding Source: UniProtKB

GO - Biological processi

  • activation of JAK1 kinase activity Source: UniProtKB
  • immune system process Source: UniProtKB-KW
  • mitotic metaphase plate congression Source: UniProtKB
  • mitotic spindle assembly Source: UniProtKB
  • negative regulation of interleukin-12 production Source: UniProtKB
  • negative regulation of natural killer cell activation Source: UniProtKB
  • negative regulation of prostaglandin biosynthetic process Source: UniProtKB
  • negative regulation of tyrosine phosphorylation of Stat4 protein Source: UniProtKB
  • non-motile cilium assembly Source: UniProtKB
  • positive regulation of interleukin-10 production Source: UniProtKB
  • positive regulation of tyrosine phosphorylation of Stat6 protein Source: UniProtKB
  • protein localization to centrosome Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Progesterone-induced-blocking factor 1
Short name:
PIBF
Alternative name(s):
Centrosomal protein of 90 kDa
Short name:
CEP901 Publication
Gene namesi
Name:PIBF1
Synonyms:C13orf24, PIBF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

HGNCiHGNC:23352. PIBF1.

Subcellular locationi

Isoform 1 :
Isoform 4 :

GO - Cellular componenti

  • centriolar satellite Source: UniProtKB
  • centrosome Source: UniProtKB
  • extracellular space Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus, Secreted

Pathology & Biotechi

Involvement in diseasei

May be associated with microcephaly.

Organism-specific databases

DisGeNETi10464.
OpenTargetsiENSG00000083535.
PharmGKBiPA162399421.

Polymorphism and mutation databases

BioMutaiPIBF1.
DMDMi73920251.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000584261 – 757Progesterone-induced-blocking factor 1Add BLAST757

Proteomic databases

EPDiQ8WXW3.
MaxQBiQ8WXW3.
PaxDbiQ8WXW3.
PeptideAtlasiQ8WXW3.
PRIDEiQ8WXW3.

PTM databases

iPTMnetiQ8WXW3.
PhosphoSitePlusiQ8WXW3.

Expressioni

Tissue specificityi

Expressed at highest levels in testis. Moderate expression is detected in spleen, thymus, prostate, ovary, small intestine, and colon (PubMed:11935316). Expressed in the first trimester pregnancy decidua (PubMed:12516630). Localized to extravillous cytotrophoblast (at protein level). Also found in syncytiotrophoblast and part of the villous cytotrophoblast. Isoform 1 is expressed in first trimester and term villous trophoblast; trophoblast cells can additionally express other isoforms (PubMed:18817979). Overexpresed in solid tumors from stomach and uterus and in cells from ovary, cervical, breast, lymphoma and leukemia cancer (PubMed:25218441).1 Publication3 Publications

Inductioni

By progesterone.1 Publication

Gene expression databases

BgeeiENSG00000083535.
CleanExiHS_PIBF1.
ExpressionAtlasiQ8WXW3. baseline and differential.
GenevisibleiQ8WXW3. HS.

Organism-specific databases

HPAiHPA052269.
HPA066120.
HPA066125.

Interactioni

Subunit structurei

Isoform 1 interacts with PCM1, BBS4 and CEP63 (PubMed:21224392, PubMed:23110211, PubMed:26297806). Interacts with IL4R (PubMed:25218441).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HTTP428583EBI-2558770,EBI-466029

GO - Molecular functioni

  • interleukin-4 receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi115727. 55 interactors.
DIPiDIP-56669N.
IntActiQ8WXW3. 50 interactors.
STRINGi9606.ENSP00000317144.

Structurei

3D structure databases

ProteinModelPortaliQ8WXW3.
SMRiQ8WXW3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 419Mediates modulation of Th2 dominant cytokine production1 PublicationAdd BLAST419
Regioni1 – 184Sufficient for NK inhibitory function1 PublicationAdd BLAST184
Regioni271 – 363Required for interaction with PCM11 PublicationAdd BLAST93

Phylogenomic databases

eggNOGiENOG410IFCG. Eukaryota.
ENOG410YQJ9. LUCA.
GeneTreeiENSGT00390000015293.
HOGENOMiHOG000006812.
HOVERGENiHBG053599.
InParanoidiQ8WXW3.
KOiK16538.
OMAiEASHIIQ.
OrthoDBiEOG091G02OD.
PhylomeDBiQ8WXW3.
TreeFamiTF329068.

Family and domain databases

InterProiIPR026205. PIBF1.
[Graphical view]
PANTHERiPTHR18950. PTHR18950. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q8WXW3-1) [UniParc]FASTAAdd to basket
Also known as: 90 kDa form

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRKISKESK KVNISSSLES EDISLETTVP TDDISSSEER EGKVRITRQL
60 70 80 90 100
IERKELLHNI QLLKIELSQK TMMIDNLKVD YLTKIEELEE KLNDALHQKQ
110 120 130 140 150
LLTLRLDNQL AFQQKDASKY QELMKQEMET ILLRQKQLEE TNLQLREKAG
160 170 180 190 200
DVRRNLRDFE LTEEQYIKLK AFPEDQLSIP EYVSVRFYEL VNPLRKEICE
210 220 230 240 250
LQVKKNILAE ELSTNKNQLK QLTETYEEDR KNYSEVQIRC QRLALELADT
260 270 280 290 300
KQLIQQGDYR QENYDKVKSE RDALEQEVIE LRRKHEILEA SHMIQTKERS
310 320 330 340 350
ELSKEVVTLE QTVTLLQKDK EYLNRQNMEL SVRCAHEEDR LERLQAQLEE
360 370 380 390 400
SKKAREEMYE KYVASRDHYK TEYENKLHDE LEQIRLKTNQ EIDQLRNASR
410 420 430 440 450
EMYERENRNL REARDNAVAE KERAVMAEKD ALEKHDQLLD RYRELQLSTE
460 470 480 490 500
SKVTEFLHQS KLKSFESERV QLLQEETARN LTQCQLECEK YQKKLEVLTK
510 520 530 540 550
EFYSLQASSE KRITELQAQN SEHQARLDIY EKLEKELDEI IMQTAEIENE
560 570 580 590 600
DEAERVLFSY GYGANVPTTA KRRLKQSVHL ARRVLQLEKQ NSLILKDLEH
610 620 630 640 650
RKDQVTQLSQ ELDRANSLLN QTQQPYRYLI ESVRQRDSKI DSLTESIAQL
660 670 680 690 700
EKDVSNLNKE KSALLQTKNQ MALDLEQLLN HREELAAMKQ ILVKMHSKHS
710 720 730 740 750
ENSLLLTKTE PKHVTENQKS KTLNVPKEHE DNIFTPKPTL FTKKEAPEWS

KKQKMKT
Length:757
Mass (Da):89,805
Last modified:August 30, 2005 - v2
Checksum:iB469FB3BCC7E80FD
GO
Isoform 2 (identifier: Q8WXW3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-541: Missing.

Note: No experimental confirmation available.
Show »
Length:216
Mass (Da):25,125
Checksum:i83A174B20DF2240F
GO
Isoform 3 (identifier: Q8WXW3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     101-103: LLT → QTF
     104-757: Missing.

Note: No experimental confirmation available.
Show »
Length:103
Mass (Da):12,016
Checksum:iDBD92AF3CD8037CB
GO
Isoform 4 (identifier: Q8WXW3-4) [UniParc]FASTAAdd to basket
Also known as: 34/35 kDa form

The sequence of this isoform differs from the canonical sequence as follows:
     225-683: Missing.

Show »
Length:298
Mass (Da):35,071
Checksum:i87F4C162AF5BEB52
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti155N → S in CAA70844 (Ref. 3) Curated1
Sequence conflicti333R → C in CAA70844 (Ref. 3) Curated1
Sequence conflicti597 – 614DLEHR…QELDR → RSGTSKGPSNTAFTRSLTE in AAQ67659 (Ref. 3) CuratedAdd BLAST18
Sequence conflicti610Q → P in AAL37481 (PubMed:11935316).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_07395289E → Q Probable disease-associated mutation found in patients with microcephaly and intellectual disability; disrupts interaction with CEP63; no effect on interaction with PCM1. 1 Publication1
Natural variantiVAR_051279167I → V.Corresponds to variant rs1372000dbSNPEnsembl.1
Natural variantiVAR_051280405R → Q.Corresponds to variant rs17089782dbSNPEnsembl.1
Natural variantiVAR_051281630I → V.Corresponds to variant rs11544631dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0153021 – 541Missing in isoform 2. 1 PublicationAdd BLAST541
Alternative sequenceiVSP_015303101 – 103LLT → QTF in isoform 3. 1 Publication3
Alternative sequenceiVSP_015304104 – 757Missing in isoform 3. 1 PublicationAdd BLAST654
Alternative sequenceiVSP_057956225 – 683Missing in isoform 4. 1 PublicationAdd BLAST459

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF330046 mRNA. Translation: AAL37481.1.
AY375528 mRNA. Translation: AAQ67659.1.
AY370776 mRNA. Translation: AAQ73282.1.
Y09631 Genomic DNA. Translation: CAA70844.1.
AL354720, AL391384 Genomic DNA. Translation: CAI14869.1.
AL391384, AL354720 Genomic DNA. Translation: CAI15673.1.
BC051911 mRNA. Translation: AAH51911.1.
CCDSiCCDS31991.1. [Q8WXW3-1]
RefSeqiNP_006337.2. NM_006346.2. [Q8WXW3-1]
UniGeneiHs.441926.

Genome annotation databases

EnsembliENST00000326291; ENSP00000317144; ENSG00000083535. [Q8WXW3-1]
ENST00000615625; ENSP00000483286; ENSG00000083535. [Q8WXW3-2]
GeneIDi10464.
KEGGihsa:10464.
UCSCiuc001vjc.4. human. [Q8WXW3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF330046 mRNA. Translation: AAL37481.1.
AY375528 mRNA. Translation: AAQ67659.1.
AY370776 mRNA. Translation: AAQ73282.1.
Y09631 Genomic DNA. Translation: CAA70844.1.
AL354720, AL391384 Genomic DNA. Translation: CAI14869.1.
AL391384, AL354720 Genomic DNA. Translation: CAI15673.1.
BC051911 mRNA. Translation: AAH51911.1.
CCDSiCCDS31991.1. [Q8WXW3-1]
RefSeqiNP_006337.2. NM_006346.2. [Q8WXW3-1]
UniGeneiHs.441926.

3D structure databases

ProteinModelPortaliQ8WXW3.
SMRiQ8WXW3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115727. 55 interactors.
DIPiDIP-56669N.
IntActiQ8WXW3. 50 interactors.
STRINGi9606.ENSP00000317144.

PTM databases

iPTMnetiQ8WXW3.
PhosphoSitePlusiQ8WXW3.

Polymorphism and mutation databases

BioMutaiPIBF1.
DMDMi73920251.

Proteomic databases

EPDiQ8WXW3.
MaxQBiQ8WXW3.
PaxDbiQ8WXW3.
PeptideAtlasiQ8WXW3.
PRIDEiQ8WXW3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000326291; ENSP00000317144; ENSG00000083535. [Q8WXW3-1]
ENST00000615625; ENSP00000483286; ENSG00000083535. [Q8WXW3-2]
GeneIDi10464.
KEGGihsa:10464.
UCSCiuc001vjc.4. human. [Q8WXW3-1]

Organism-specific databases

CTDi10464.
DisGeNETi10464.
GeneCardsiPIBF1.
HGNCiHGNC:23352. PIBF1.
HPAiHPA052269.
HPA066120.
HPA066125.
MIMi607532. gene.
neXtProtiNX_Q8WXW3.
OpenTargetsiENSG00000083535.
PharmGKBiPA162399421.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFCG. Eukaryota.
ENOG410YQJ9. LUCA.
GeneTreeiENSGT00390000015293.
HOGENOMiHOG000006812.
HOVERGENiHBG053599.
InParanoidiQ8WXW3.
KOiK16538.
OMAiEASHIIQ.
OrthoDBiEOG091G02OD.
PhylomeDBiQ8WXW3.
TreeFamiTF329068.

Miscellaneous databases

ChiTaRSiPIBF1. human.
GeneWikiiC13orf24.
GenomeRNAii10464.
PROiQ8WXW3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000083535.
CleanExiHS_PIBF1.
ExpressionAtlasiQ8WXW3. baseline and differential.
GenevisibleiQ8WXW3. HS.

Family and domain databases

InterProiIPR026205. PIBF1.
[Graphical view]
PANTHERiPTHR18950. PTHR18950. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPIBF1_HUMAN
AccessioniPrimary (citable) accession number: Q8WXW3
Secondary accession number(s): O95664
, Q6U9V2, Q6UG50, Q86V07, Q96SF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: August 30, 2005
Last modified: November 30, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

During normal pregnancy, the production is continuously increasing until the 37th gestational week and is followed by a sharp decrease after the 41st week of gestation. In pathological pregnancies, urinary levels fail to increase. Candidate for the diagnosis of threatened premature pregnancy termination.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.