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Q8WXS8 (ATS14_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
A disintegrin and metalloproteinase with thrombospondin motifs 14

Short name=ADAM-TS 14
Short name=ADAM-TS14
Short name=ADAMTS-14
EC=3.4.24.-
Gene names
Name:ADAMTS14
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1223 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Has a aminoprocollagen type I activity processing activity in the absence of ADAMTS2. Seems to be synthesized as a latent enzyme that requires activation to display aminoprocollagen peptidase activity.

Subcellular location

Secretedextracellular spaceextracellular matrix By similarity.

Tissue specificity

Expressed in retina and at low levels in brain, lung and placenta. High expression in fetal tissues.

Domain

The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix By similarity.

Post-translational modification

The precursor is cleaved by a furin endopeptidase By similarity.

Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion By similarity.

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 peptidase M12B domain.

Contains 1 PLAC domain.

Contains 4 TSP type-1 domains.

Alternative products

This entry describes 4 isoforms produced by alternative promoter usage and alternative splicing. [Align] [Select]
Isoform A (identifier: Q8WXS8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by alternative promoter usage.
Isoform B (identifier: Q8WXS8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-67: Missing.
Note: Produced by alternative promoter usage.
Isoform C (identifier: Q8WXS8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-67: Missing.
     368-368: G → GMQG
Note: Produced by alternative splicing of isoform B.
Isoform D (identifier: Q8WXS8-4)

The sequence of this isoform differs from the canonical sequence as follows:
     368-368: G → GMQG
Note: Produced by alternative splicing of isoform A.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Propeptide23 – 252230 By similarity
PRO_0000029190
Chain253 – 1223971A disintegrin and metalloproteinase with thrombospondin motifs 14
PRO_0000029191

Regions

Domain259 – 460202Peptidase M12B
Domain461 – 55191Disintegrin
Domain552 – 60756TSP type-1 1
Domain847 – 90761TSP type-1 2
Domain908 – 96760TSP type-1 3
Domain968 – 102255TSP type-1 4
Domain1059 – 109739PLAC
Region730 – 846117Spacer
Compositional bias608 – 729122Cys-rich
Compositional bias875 – 8784Poly-Arg
Compositional bias1100 – 1221122Pro-rich

Sites

Active site3991 By similarity
Metal binding3981Zinc; catalytic By similarity
Metal binding4021Zinc; catalytic By similarity
Metal binding4081Zinc; catalytic By similarity

Amino acid modifications

Glycosylation1091N-linked (GlcNAc...) Potential
Glycosylation4751N-linked (GlcNAc...) Potential
Glycosylation9411N-linked (GlcNAc...) Potential
Glycosylation10271N-linked (GlcNAc...) Potential
Disulfide bond336 ↔ 382 By similarity
Disulfide bond376 ↔ 455 By similarity
Disulfide bond415 ↔ 441 By similarity
Disulfide bond482 ↔ 507 By similarity
Disulfide bond493 ↔ 516 By similarity
Disulfide bond502 ↔ 535 By similarity
Disulfide bond529 ↔ 540 By similarity
Disulfide bond564 ↔ 601 By similarity
Disulfide bond568 ↔ 606 By similarity
Disulfide bond579 ↔ 591 By similarity
Disulfide bond980 ↔ 1016 By similarity
Disulfide bond984 ↔ 1021 By similarity
Disulfide bond995 ↔ 1005 By similarity

Natural variations

Alternative sequence1 – 6767Missing in isoform B and isoform C.
VSP_006958
Alternative sequence3681G → GMQG in isoform C and isoform D.
VSP_005501
Natural variant1791R → C.
Corresponds to variant rs34022601 [ dbSNP | Ensembl ].
VAR_047837
Natural variant5901L → P. Ref.1 Ref.2 Ref.5
Corresponds to variant rs10823607 [ dbSNP | Ensembl ].
VAR_047838
Natural variant9371L → M.
Corresponds to variant rs12774070 [ dbSNP | Ensembl ].
VAR_047839
Natural variant10171S → N.
Corresponds to variant rs10999516 [ dbSNP | Ensembl ].
VAR_047840
Natural variant10491E → G.
Corresponds to variant rs4747096 [ dbSNP | Ensembl ].
VAR_047841

Experimental info

Sequence conflict8681Q → R in CAC87943. Ref.2
Sequence conflict8841Q → H in CAC87943. Ref.2
Sequence conflict9011C → S in CAC87943. Ref.2
Sequence conflict9231C → Y in CAC87943. Ref.2
Sequence conflict10241N → S in CAC87943. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified January 11, 2011. Version 2.
Checksum: EEF3229519B3170A

FASTA1,223133,888
        10         20         30         40         50         60 
MAPLRALLSY LLPLHCALCA AAGSRTPELH LSGKLSDYGV TVPCSTDFRG RFLSHVVSGP 

        70         80         90        100        110        120 
AAASAGSMVV DTPPTLPRHS SHLRVARSPL HPGGTLWPGR VGRHSLYFNV TVFGKELHLR 

       130        140        150        160        170        180 
LRPNRRLVVP GSSVEWQEDF RELFRQPLRQ ECVYTGGVTG MPGAAVAISN CDGLAGLIRT 

       190        200        210        220        230        240 
DSTDFFIEPL ERGQQEKEAS GRTHVVYRRE AVQQEWAEPD GDLHNEAFGL GDLPNLLGLV 

       250        260        270        280        290        300 
GDQLGDTERK RRHAKPGSYS IEVLLVVDDS VVRFHGKEHV QNYVLTLMNI VDEIYHDESL 

       310        320        330        340        350        360 
GVHINIALVR LIMVGYRQSL SLIERGNPSR SLEQVCRWAH SQQRQDPSHA EHHDHVVFLT 

       370        380        390        400        410        420 
RQDFGPSGYA PVTGMCHPLR SCALNHEDGF SSAFVIAHET GHVLGMEHDG QGNGCADETS 

       430        440        450        460        470        480 
LGSVMAPLVQ AAFHRFHWSR CSKLELSRYL PSYDCLLDDP FDPAWPQPPE LPGINYSMDE 

       490        500        510        520        530        540 
QCRFDFGSGY QTCLAFRTFE PCKQLWCSHP DNPYFCKTKK GPPLDGTECA PGKWCFKGHC 

       550        560        570        580        590        600 
IWKSPEQTYG QDGGWSSWTK FGSCSRSCGG GVRSRSRSCN NPSPAYGGRL CLGPMFEYQV 

       610        620        630        640        650        660 
CNSEECPGTY EDFRAQQCAK RNSYYVHQNA KHSWVPYEPD DDAQKCELIC QSADTGDVVF 

       670        680        690        700        710        720 
MNQVVHDGTR CSYRDPYSVC ARGECVPVGC DKEVGSMKAD DKCGVCGGDN SHCRTVKGTL 

       730        740        750        760        770        780 
GKASKQAGAL KLVQIPAGAR HIQIEALEKS PHRIVVKNQV TGSFILNPKG KEATSRTFTA 

       790        800        810        820        830        840 
MGLEWEDAVE DAKESLKTSG PLPEAIAILA LPPTEGGPRS SLAYKYVIHE DLLPLIGSNN 

       850        860        870        880        890        900 
VLLEEMDTYE WALKSWAPCS KACGGGIQFT KYGCRRRRDH HMVQRHLCDH KKRPKPIRRR 

       910        920        930        940        950        960 
CNQHPCSQPV WVTEEWGACS RSCGKLGVQT RGIQCLLPLS NGTHKVMPAK ACAGDRPEAR 

       970        980        990       1000       1010       1020 
RPCLRVPCPA QWRLGAWSQC SATCGEGIQQ RQVVCRTNAN SLGHCEGDRP DTVQVCSLPA 

      1030       1040       1050       1060       1070       1080 
CGGNHQNSTV RADVWELGTP EGQWVPQSEP LHPINKISST EPCTGDRSVF CQMEVLDRYC 

      1090       1100       1110       1120       1130       1140 
SIPGYHRLCC VSCIKKASGP NPGPDPGPTS LPPFSTPGSP LPGPQDPADA AEPPGKPTGS 

      1150       1160       1170       1180       1190       1200 
EDHQHGRATQ LPGALDTSSP GTQHPFAPET PIPGASWSIS PTTPGGLPWG WTQTPTPVPE 

      1210       1220 
DKGQPGEDLR HPGTSLPAAS PVT 

« Hide

Isoform B [UniParc].

Checksum: 0EF756C57D635CAE
Show »

FASTA1,156127,036
Isoform C [UniParc].

Checksum: 7D7B58455B6BFF31
Show »

FASTA1,159127,353
Isoform D [UniParc].

Checksum: D218E0655F1FAF55
Show »

FASTA1,226134,205

References

« Hide 'large scale' references
[1]"Characterization of ADAMTS14, a novel member of the ADAMTS metalloproteinase family."
Bolz H., Ramirez A., von Brederlow B., Kubisch C.
Biochim. Biophys. Acta 1522:221-225(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), VARIANT PRO-590.
[2]"Cloning, expression analysis, and structural characterization of seven novel human ADAMTSs, a family of metalloproteinases with disintegrin and thrombospondin-1 domains."
Cal S., Obaya A.J., Llamazares M., Garabaya C., Quesada V., Lopez-Otin C.
Gene 283:49-62(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), VARIANT PRO-590.
Tissue: Fetal lung.
[3]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Cloning and characterization of ADAMTS-14, a novel ADAMTS displaying high homology with ADAMTS-2 and ADAMTS-3."
Colige A., Vandenberghe I., Thiry M., Lambert C.A., Van Beeumen J., Li S.-W., Prockop D.J., Lapiere C.M., Nusgens B.V.
J. Biol. Chem. 277:5756-5766(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 29-1223 (ISOFORMS B; C AND D), ALTERNATIVE PROMOTER USAGE, VARIANT PRO-590.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF358666 mRNA. Translation: AAL40229.1.
AJ345098 mRNA. Translation: CAC87943.1.
AL358817, AL355344 Genomic DNA. Translation: CAI13857.1.
AL358817, AL355344 Genomic DNA. Translation: CAI13858.1.
AL355344, AL358817 Genomic DNA. Translation: CAI41277.1.
AL355344, AL358817 Genomic DNA. Translation: CAI41278.1.
CH471083 Genomic DNA. Translation: EAW54409.1.
CH471083 Genomic DNA. Translation: EAW54410.1.
AF366351 mRNA. Translation: AAL79814.1.
RefSeqNP_542453.2. NM_080722.3.
NP_631894.2. NM_139155.2.
UniGeneHs.352156.

3D structure databases

ProteinModelPortalQ8WXS8.
SMRQ8WXS8. Positions 261-830, 852-1022.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000362304.

Protein family/group databases

MEROPSM12.024.

PTM databases

PhosphoSiteQ8WXS8.

Polymorphism databases

DMDM317373325.

Proteomic databases

PaxDbQ8WXS8.
PRIDEQ8WXS8.

Protocols and materials databases

DNASU140766.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000373207; ENSP00000362303; ENSG00000138316. [Q8WXS8-1]
ENST00000373208; ENSP00000362304; ENSG00000138316. [Q8WXS8-4]
GeneID140766.
KEGGhsa:140766.
UCSCuc001jrg.3. human. [Q8WXS8-4]
uc001jrh.3. human. [Q8WXS8-1]

Organism-specific databases

CTD140766.
GeneCardsGC10P072432.
H-InvDBHIX0025921.
HGNCHGNC:14899. ADAMTS14.
HPAHPA034605.
MIM607506. gene.
neXtProtNX_Q8WXS8.
PharmGKBPA24540.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG307411.
HOGENOMHOG000034222.
HOVERGENHBG004314.
KOK08628.
OMALPSYDCL.
OrthoDBEOG76HQ0N.
PhylomeDBQ8WXS8.
TreeFamTF313537.

Enzyme and pathway databases

ReactomeREACT_118779. Extracellular matrix organization.

Gene expression databases

BgeeQ8WXS8.
CleanExHS_ADAMTS14.
GenevestigatorQ8WXS8.

Family and domain databases

Gene3D3.40.390.10. 1 hit.
InterProIPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR010909. PLAC.
IPR000884. Thrombospondin_1_rpt.
[Graphical view]
PfamPF05986. ADAM_spacer1. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 4 hits.
[Graphical view]
PRINTSPR01857. ADAMTSFAMILY.
SMARTSM00209. TSP1. 4 hits.
[Graphical view]
SUPFAMSSF82895. SSF82895. 4 hits.
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS50900. PLAC. 1 hit.
PS50092. TSP1. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi140766.
NextBio84365.
PROQ8WXS8.
SOURCESearch...

Entry information

Entry nameATS14_HUMAN
AccessionPrimary (citable) accession number: Q8WXS8
Secondary accession number(s): Q5T4G0 expand/collapse secondary AC list , Q5T4G1, Q8TE55, Q8TEY8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: January 11, 2011
Last modified: April 16, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM