Q8WXS5 (CCG8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Voltage-dependent calcium channel gamma-8 subunit

Alternative name(s):
Neuronal voltage-gated calcium channel gamma-8 subunit
Transmembrane AMPAR regulatory protein gamma-8
Short name=TARP gamma-8

Gene names

Name:	CACNG8
Synonyms:	CACNG6

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization and by mediating their resensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state. Ref.5 Ref.6
Subunit structure	The L-type calcium channel is composed of five subunits: alpha-1, alpha-2/delta, beta and gamma. Acts as an auxiliary subunit for AMPA-selective glutamate receptors (AMPARs). Found in a complex with GRIA1, GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5 and CACNG7. Interacts with CNIH2 By similarity.
Subcellular location	Membrane; Multi-pass membrane protein By similarity. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density By similarity.
Sequence similarities	Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.
Sequence caution	The sequence AAK15019.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the N-terminal part. The sequence AAL50049.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane Transmembrane helix
Ligand	Calcium
Molecular function	Calcium channel Ion channel Voltage-gated channel
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Inferred from direct assay Ref.6Ref.5. Source: UniProtKB synaptic transmission Traceable author statement. Source: Reactome
Cellular_component	alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex Inferred from sequence or structural similarity. Source: UniProtKB cell junction Inferred from electronic annotation. Source: UniProtKB-KW endocytic vesicle membrane Traceable author statement. Source: Reactome postsynaptic density Inferred from sequence or structural similarity. Source: UniProtKB postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW voltage-gated calcium channel complex Non-traceable author statement Ref.4. Source: UniProtKB
Molecular_function	voltage-gated calcium channel activity Non-traceable author statement Ref.4. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Voltage-dependent calcium channel gamma-8 subunit		PRO_0000164690
Regions
	Transmembrane	19 – 39	21	Helical; Potential
	Transmembrane	129 – 149	21	Helical; Potential
	Transmembrane	158 – 178	21	Helical; Potential
	Transmembrane	208 – 228	21	Helical; Potential
	Transmembrane	318 – 338	21	Helical; Potential
	Compositional bias	247 – 380	134	Gly-rich
Amino acid modifications
	Modified residue	252	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	16	1	E → K in AAK15019. Ref.3
	Sequence conflict	362	1	A → S in AAL50049. Ref.1
	Sequence conflict	386	1	G → R in AAL50049. Ref.1
	Sequence conflict	394	1	A → T in AAL50049. Ref.1
	Sequence conflict	409	1	E → G in AAL50049. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8WXS5 [UniParc]. Last modified November 30, 2010. Version 3. Checksum: E8C33CC5CF6184A1	FASTA	425	43,313
	10 20 30 40 50 60 MESLKRWNEE RGLWCEKGVQ VLLTTVGAFA AFGLMTIAIS TDYWLYTRAL ICNTTNLTAG 70 80 90 100 110 120 GDDGTPHRGG GGASEKKDPG GLTHSGLWRI CCLEGLKRGV CVKINHFPED TDYDHDSAEY 130 140 150 160 170 180 LLRVVRASSI FPILSAILLL LGGVCVAASR VYKSKRNIIL GAGILFVAAG LSNIIGVIVY 190 200 210 220 230 240 ISANAGEPGP KRDEEKKNHY SYGWSFYFGG LSFILAEVIG VLAVNIYIER SREAHCQSRS 250 260 270 280 290 300 DLLKAGGGAG GSGGSGPSAI LRLPSYRFRY RRRSRSSSRS SEPSPSRDAS PGGPGGPGFA 310 320 330 340 350 360 STDISMYTLS RDPSKGSVAA GLAGAGGGGG GAVGAFGGAA GGAGGGGGGG GGAGAERDRG 370 380 390 400 410 420 GASGFLTLHN AFPKEAGGGV TVTVTGPPAP PAPAPPAPSA PAPGTLAKEA AASNTNTLNR KTTPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Calcium channel gamma subunits provide insights into the evolution of this gene family." Chu P.-J., Robertson H.M., Best P.M. Gene 280:37-48(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Proposed Homo sapiens voltage-gated calcium channel gamma-6 subunit." Black J.L. III, Kryzer T.J., Lennon V.A. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-204. Tissue: Cerebellum.
[4]	"A cluster of three novel Ca(2+) channel gamma subunit genes on chromosome 19q13.4: evolution and expression profile of the gamma subunit gene family." Burgess D.L., Gefrides L.A., Foreman P.J., Noebels J.L. Genomics 71:339-350(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-425.
[5]	"Hippocampal AMPA receptor gating controlled by both TARP and cornichon proteins." Kato A.S., Gill M.B., Ho M.T., Yu H., Tu Y., Siuda E.R., Wang H., Qian Y.W., Nisenbaum E.S., Tomita S., Bredt D.S. Neuron 68:1082-1096(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Functional comparison of the effects of TARPs and cornichons on AMPA receptor trafficking and gating." Shi Y., Suh Y.H., Milstein A.D., Isozaki K., Schmid S.M., Roche K.W., Nicoll R.A. Proc. Natl. Acad. Sci. U.S.A. 107:16315-16319(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF361354 mRNA. Translation: AAL50049.1. Frameshift. AC008440 Genomic DNA. No translation available. AF234892 mRNA. Translation: AAK15019.1. Sequence problems. AF288388 mRNA. Translation: AAK20031.1.
IPI	IPI00103604.
RefSeq	NP_114101.4. NM_031895.5.
UniGene	Hs.631595.
3D structure databases
ProteinModelPortal	Q8WXS5.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000270458.
Protein family/group databases
TCDB	8.A.16.2.6. Ca+ channel auxiliary subunit gamma1-gamma8 (CCAgamma) family.
PTM databases
PhosphoSite	Q8WXS5.
Polymorphism databases
DMDM	20532017.
Proteomic databases
PaxDb	Q8WXS5.
PRIDE	Q8WXS5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000270458; ENSP00000270458; ENSG00000142408.
GeneID	59283.
KEGG	hsa:59283.
UCSC	uc002qcs.2. human.
Organism-specific databases
CTD	59283.
GeneCards	GC19P054466.
H-InvDB	HIX0202898.
HGNC	HGNC:13628. CACNG8.
HPA	HPA041351.
MIM	606900. gene.
neXtProt	NX_Q8WXS5.
PharmGKB	PA26022.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG43469.
HOGENOM	HOG000233440.
HOVERGEN	HBG003682.
InParanoid	Q8WXS5.
KO	K04873.
OrthoDB	EOG4RXZ0W.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System.
Gene expression databases
Bgee	Q8WXS5.
CleanEx	HS_CACNG6. HS_CACNG8.
Genevestigator	Q8WXS5.
GermOnline	ENSG00000142408. Homo sapiens.
Family and domain databases
InterPro	IPR004031. PMP22/EMP/MP20/Claudin. IPR008372. VDCC_g8su. IPR008368. VDCC_gsu. [Graphical view]
Pfam	PF00822. PMP22_Claudin. 1 hit. [Graphical view]
PRINTS	PR01792. VDCCGAMMA. PR01796. VDCCGAMMA8.
ProtoNet	Search...
Other
ChiTaRS	CACNG8. human.
GenomeRNAi	59283.
NextBio	65170.
SOURCE	Search...

Entry information

Entry name

CCG8_HUMAN

Accession

Primary (citable) accession number: Q8WXS5
Secondary accession number(s): Q9BXT0, Q9BY23

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	November 30, 2010
Last modified:	April 3, 2013
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.