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Protein

Carboxypeptidase A5

Gene

CPA5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi196 – 1961Zinc; catalyticBy similarity
Metal bindingi199 – 1991Zinc; catalyticBy similarity
Binding sitei254 – 2541SubstrateBy similarity
Metal bindingi323 – 3231Zinc; catalyticBy similarity
Active sitei397 – 3971NucleophileBy similarity

GO - Molecular functioni

  1. metallocarboxypeptidase activity Source: InterPro
  2. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM14.020.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase A5 (EC:3.4.17.-)
Gene namesi
Name:CPA5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 7

Organism-specific databases

HGNCiHGNC:15722. CPA5.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26820.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence AnalysisAdd
BLAST
Propeptidei34 – 12693Activation peptideBy similarityPRO_0000004361Add
BLAST
Chaini127 – 436310Carboxypeptidase A5PRO_0000004362Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi265 ↔ 288By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Zymogen

Proteomic databases

PaxDbiQ8WXQ8.
PRIDEiQ8WXQ8.

Expressioni

Tissue specificityi

Expression is very low or not detectable.

Gene expression databases

BgeeiQ8WXQ8.
CleanExiHS_CPA5.
ExpressionAtlasiQ8WXQ8. baseline and differential.
GenevestigatoriQ8WXQ8.

Organism-specific databases

HPAiHPA020322.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000347549.

Structurei

3D structure databases

ProteinModelPortaliQ8WXQ8.
SMRiQ8WXQ8. Positions 37-436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni196 – 1994Substrate bindingBy similarity
Regioni271 – 2722Substrate bindingBy similarity
Regioni324 – 3252Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2866.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252967.
HOVERGENiHBG050815.
InParanoidiQ8WXQ8.
KOiK08781.
OMAiVHGIEYI.
PhylomeDBiQ8WXQ8.
TreeFamiTF317197.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR000834. Peptidase_M14.
IPR003146. Prot_inh_M14A.
IPR009020. Prot_inh_propept.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WXQ8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQGTPGGGTR PGPSPVDRRT LLVFSFILAA ALGQMNFTGD QVLRVLAKDE
60 70 80 90 100
KQLSLLGDLE GLKPQKVDFW RGPARPSLPV DMRVPFSELK DIKAYLESHG
110 120 130 140 150
LAYSIMIKDI QVLLDEERQA MAKSRRLERS TNSFSYSSYH TLEEIYSWID
160 170 180 190 200
NFVMEHSDIV SKIQIGNSFE NQSILVLKFS TGGSRHPAIW IDTGIHSREW
210 220 230 240 250
ITHATGIWTA NKIVSDYGKD RVLTDILNAM DIFIELVTNP DGFAFTHSMN
260 270 280 290 300
RLWRKNKSIR PGIFCIGVDL NRNWKSGFGG NGSNSNPCSE TYHGPSPQSE
310 320 330 340 350
PEVAAIVNFI TAHGNFKALI SIHSYSQMLM YPYGRLLEPV SNQRELYDLA
360 370 380 390 400
KDAVEALYKV HGIEYIFGSI STTLYVASGI TVDWAYDSGI KYAFSFELRD
410 420 430
TGQYGFLLPA TQIIPTAQET WMALRTIMEH TLNHPY
Length:436
Mass (Da):49,036
Last modified:March 1, 2002 - v1
Checksum:iA6EB4FFDE72F355A
GO
Isoform 2 (identifier: Q8WXQ8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     347-351: YDLAK → VRLAA
     352-436: Missing.

Note: No experimental confirmation available.

Show »
Length:351
Mass (Da):39,364
Checksum:i9D083276FD2D6B16
GO
Isoform 3 (identifier: Q8WXQ8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     347-436: YDLAKDAVEA...IMEHTLNHPY → MWPVGSPSTG...WRFGPSWSTP

Note: No experimental confirmation available.

Show »
Length:403
Mass (Da):44,881
Checksum:i3C8467B533BAB019
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti108 – 1081K → R in BAC04122 (PubMed:14702039).Curated
Sequence conflicti117 – 1171E → G in AAH39362 (PubMed:15489334).Curated
Sequence conflicti293 – 2931H → R in BAD18389 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti79 – 791P → S.1 Publication
Corresponds to variant rs17388190 [ dbSNP | Ensembl ].
VAR_017191
Natural varianti336 – 3361L → S.2 Publications
Corresponds to variant rs11761888 [ dbSNP | Ensembl ].
VAR_017192
Natural varianti338 – 3381E → D.2 Publications
Corresponds to variant rs17854248 [ dbSNP | Ensembl ].
VAR_017193
Natural varianti378 – 3781S → G.
Corresponds to variant rs11765961 [ dbSNP | Ensembl ].
VAR_048596

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei347 – 43690YDLAK…LNHPY → MWPVGSPSTGPMTVASSTPS ALSSGTLGSMASCCRPHRSS PRPRRRGWRFGPSWSTP in isoform 3. 1 PublicationVSP_044866Add
BLAST
Alternative sequencei347 – 3515YDLAK → VRLAA in isoform 2. 1 PublicationVSP_008806
Alternative sequencei352 – 43685Missing in isoform 2. 1 PublicationVSP_008807Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF384667 mRNA. Translation: AAL37611.1.
AY155229 mRNA. Translation: AAO17155.1.
AY155230 mRNA. Translation: AAO17156.1.
AK093288 mRNA. Translation: BAC04122.1.
AK131191 mRNA. Translation: BAD18389.1.
AC007938 Genomic DNA. No translation available.
CH471070 Genomic DNA. Translation: EAW83757.1.
BC039362 mRNA. Translation: AAH39362.1.
BC042996 mRNA. Translation: AAH42996.1.
BK000187 mRNA. Translation: DAA00035.1.
CCDSiCCDS47713.1. [Q8WXQ8-3]
CCDS5819.1. [Q8WXQ8-1]
RefSeqiNP_001120913.1. NM_001127441.1. [Q8WXQ8-1]
NP_001120914.1. NM_001127442.1. [Q8WXQ8-3]
NP_525124.3. NM_080385.4. [Q8WXQ8-1]
XP_005250767.1. XM_005250710.1. [Q8WXQ8-1]
XP_005250768.1. XM_005250711.1. [Q8WXQ8-1]
XP_005250769.1. XM_005250712.1. [Q8WXQ8-1]
XP_006716241.1. XM_006716178.1. [Q8WXQ8-3]
UniGeneiHs.567642.

Genome annotation databases

EnsembliENST00000393213; ENSP00000376907; ENSG00000158525. [Q8WXQ8-1]
ENST00000431780; ENSP00000393045; ENSG00000158525. [Q8WXQ8-3]
ENST00000461828; ENSP00000418183; ENSG00000158525. [Q8WXQ8-1]
ENST00000466363; ENSP00000419025; ENSG00000158525. [Q8WXQ8-1]
ENST00000474905; ENSP00000417314; ENSG00000158525. [Q8WXQ8-1]
ENST00000485477; ENSP00000420237; ENSG00000158525. [Q8WXQ8-1]
GeneIDi93979.
KEGGihsa:93979.
UCSCiuc003vps.2. human. [Q8WXQ8-1]
uc003vpt.2. human.

Polymorphism databases

DMDMi38257690.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF384667 mRNA. Translation: AAL37611.1.
AY155229 mRNA. Translation: AAO17155.1.
AY155230 mRNA. Translation: AAO17156.1.
AK093288 mRNA. Translation: BAC04122.1.
AK131191 mRNA. Translation: BAD18389.1.
AC007938 Genomic DNA. No translation available.
CH471070 Genomic DNA. Translation: EAW83757.1.
BC039362 mRNA. Translation: AAH39362.1.
BC042996 mRNA. Translation: AAH42996.1.
BK000187 mRNA. Translation: DAA00035.1.
CCDSiCCDS47713.1. [Q8WXQ8-3]
CCDS5819.1. [Q8WXQ8-1]
RefSeqiNP_001120913.1. NM_001127441.1. [Q8WXQ8-1]
NP_001120914.1. NM_001127442.1. [Q8WXQ8-3]
NP_525124.3. NM_080385.4. [Q8WXQ8-1]
XP_005250767.1. XM_005250710.1. [Q8WXQ8-1]
XP_005250768.1. XM_005250711.1. [Q8WXQ8-1]
XP_005250769.1. XM_005250712.1. [Q8WXQ8-1]
XP_006716241.1. XM_006716178.1. [Q8WXQ8-3]
UniGeneiHs.567642.

3D structure databases

ProteinModelPortaliQ8WXQ8.
SMRiQ8WXQ8. Positions 37-436.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000347549.

Protein family/group databases

MEROPSiM14.020.

Polymorphism databases

DMDMi38257690.

Proteomic databases

PaxDbiQ8WXQ8.
PRIDEiQ8WXQ8.

Protocols and materials databases

DNASUi93979.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000393213; ENSP00000376907; ENSG00000158525. [Q8WXQ8-1]
ENST00000431780; ENSP00000393045; ENSG00000158525. [Q8WXQ8-3]
ENST00000461828; ENSP00000418183; ENSG00000158525. [Q8WXQ8-1]
ENST00000466363; ENSP00000419025; ENSG00000158525. [Q8WXQ8-1]
ENST00000474905; ENSP00000417314; ENSG00000158525. [Q8WXQ8-1]
ENST00000485477; ENSP00000420237; ENSG00000158525. [Q8WXQ8-1]
GeneIDi93979.
KEGGihsa:93979.
UCSCiuc003vps.2. human. [Q8WXQ8-1]
uc003vpt.2. human.

Organism-specific databases

CTDi93979.
GeneCardsiGC07P129986.
HGNCiHGNC:15722. CPA5.
HPAiHPA020322.
MIMi609561. gene.
neXtProtiNX_Q8WXQ8.
PharmGKBiPA26820.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG2866.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252967.
HOVERGENiHBG050815.
InParanoidiQ8WXQ8.
KOiK08781.
OMAiVHGIEYI.
PhylomeDBiQ8WXQ8.
TreeFamiTF317197.

Miscellaneous databases

GeneWikiiCPA5.
GenomeRNAii93979.
NextBioi78246.
PROiQ8WXQ8.
SOURCEiSearch...

Gene expression databases

BgeeiQ8WXQ8.
CleanExiHS_CPA5.
ExpressionAtlasiQ8WXQ8. baseline and differential.
GenevestigatoriQ8WXQ8.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR000834. Peptidase_M14.
IPR003146. Prot_inh_M14A.
IPR009020. Prot_inh_propept.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mutation screening and imprinting analysis of four candidate genes for autism in the 7q32 region."
    Bonora E., Bacchelli E., Levy E.R., Blasi F., Marlow A., Monaco A.P., Maestrini E.
    Mol. Psychiatry 7:289-301(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS SER-79; SER-336 AND ASP-338.
  2. "The imprinted region on human chromosome 7q32 extends to the carboxypeptidase A gene cluster: an imprinted candidate for Silver-Russell syndrome."
    Bentley L., Nakabayashi K., Monk D., Beechey C., Peters J., Birjandi Z., Khayat F.E., Patel M., Preece M.A., Stanier P., Scherer S.W., Moore G.E.
    J. Med. Genet. 40:249-256(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Medulla oblongata.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Tissue: Testis.
  4. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS SER-336 AND ASP-338.
    Tissue: Testis.
  7. "Identification and characterization of three members of the human metallocarboxypeptidase gene family."
    Wei S., Segura S., Vendrell J., Aviles F.X., Lanoue E., Day R., Feng Y., Fricker L.D.
    J. Biol. Chem. 277:14954-14964(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE STRUCTURE.

Entry informationi

Entry nameiCBPA5_HUMAN
AccessioniPrimary (citable) accession number: Q8WXQ8
Secondary accession number(s): G3V0G8
, Q6ZNI6, Q86SE2, Q86XM3, Q8NA08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: March 1, 2002
Last modified: January 7, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.