Q8WXQ8 (CBPA5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carboxypeptidase A5
EC=3.4.17.1

Gene names

Name:

CPA5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Expression is very low or not detectable.
Sequence similarities	Belongs to the peptidase M14 family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Carboxypeptidase Hydrolase Metalloprotease Protease
PTM	Cleavage on pair of basic residues Disulfide bond Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metallocarboxypeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8WXQ8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8WXQ8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     347-351: YDLAK → VRLAA
     352-436: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8WXQ8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     347-436: YDLAKDAVEA...IMEHTLNHPY → MWPVGSPSTG...WRFGPSWSTP

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Potential
	Propeptide	34 – 126	93	Activation peptide By similarity		PRO_0000004361
	Chain	127 – 436	310	Carboxypeptidase A5		PRO_0000004362
Regions
	Region	196 – 199	4	Substrate binding By similarity
	Region	271 – 272	2	Substrate binding By similarity
	Region	324 – 325	2	Substrate binding By similarity
Sites
	Active site	397	1	Nucleophile By similarity
	Metal binding	196	1	Zinc; catalytic By similarity
	Metal binding	199	1	Zinc; catalytic By similarity
	Metal binding	323	1	Zinc; catalytic By similarity
	Binding site	254	1	Substrate By similarity
Amino acid modifications
	Disulfide bond	265 ↔ 288		By similarity
Natural variations
	Alternative sequence	347 – 436	90	YDLAK…LNHPY → MWPVGSPSTGPMTVASSTPS ALSSGTLGSMASCCRPHRSS PRPRRRGWRFGPSWSTP in isoform 3.		VSP_044866
	Alternative sequence	347 – 351	5	YDLAK → VRLAA in isoform 2.		VSP_008806
	Alternative sequence	352 – 436	85	Missing in isoform 2.		VSP_008807
	Natural variant	79	1	P → S. Ref.1 Corresponds to variant rs17388190 [ dbSNP | Ensembl ].		VAR_017191
	Natural variant	336	1	L → S. Ref.1 Ref.6 Corresponds to variant rs11761888 [ dbSNP | Ensembl ].		VAR_017192
	Natural variant	338	1	E → D. Ref.1 Ref.6		VAR_017193
	Natural variant	378	1	S → G. Corresponds to variant rs11765961 [ dbSNP | Ensembl ].		VAR_048596
Experimental info
	Sequence conflict	108	1	K → R in BAC04122. Ref.3
	Sequence conflict	117	1	E → G in AAH39362. Ref.6
	Sequence conflict	293	1	H → R in BAD18389. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: A6EB4FFDE72F355A	FASTA	436	49,036
	10 20 30 40 50 60 MQGTPGGGTR PGPSPVDRRT LLVFSFILAA ALGQMNFTGD QVLRVLAKDE KQLSLLGDLE 70 80 90 100 110 120 GLKPQKVDFW RGPARPSLPV DMRVPFSELK DIKAYLESHG LAYSIMIKDI QVLLDEERQA 130 140 150 160 170 180 MAKSRRLERS TNSFSYSSYH TLEEIYSWID NFVMEHSDIV SKIQIGNSFE NQSILVLKFS 190 200 210 220 230 240 TGGSRHPAIW IDTGIHSREW ITHATGIWTA NKIVSDYGKD RVLTDILNAM DIFIELVTNP 250 260 270 280 290 300 DGFAFTHSMN RLWRKNKSIR PGIFCIGVDL NRNWKSGFGG NGSNSNPCSE TYHGPSPQSE 310 320 330 340 350 360 PEVAAIVNFI TAHGNFKALI SIHSYSQMLM YPYGRLLEPV SNQRELYDLA KDAVEALYKV 370 380 390 400 410 420 HGIEYIFGSI STTLYVASGI TVDWAYDSGI KYAFSFELRD TGQYGFLLPA TQIIPTAQET 430 WMALRTIMEH TLNHPY
	Isoform 2 [UniParc]. Checksum: 9D083276FD2D6B16	FASTA	351	39,364
	10 20 30 40 50 60 MQGTPGGGTR PGPSPVDRRT LLVFSFILAA ALGQMNFTGD QVLRVLAKDE KQLSLLGDLE 70 80 90 100 110 120 GLKPQKVDFW RGPARPSLPV DMRVPFSELK DIKAYLESHG LAYSIMIKDI QVLLDEERQA 130 140 150 160 170 180 MAKSRRLERS TNSFSYSSYH TLEEIYSWID NFVMEHSDIV SKIQIGNSFE NQSILVLKFS 190 200 210 220 230 240 TGGSRHPAIW IDTGIHSREW ITHATGIWTA NKIVSDYGKD RVLTDILNAM DIFIELVTNP 250 260 270 280 290 300 DGFAFTHSMN RLWRKNKSIR PGIFCIGVDL NRNWKSGFGG NGSNSNPCSE TYHGPSPQSE 310 320 330 340 350 PEVAAIVNFI TAHGNFKALI SIHSYSQMLM YPYGRLLEPV SNQRELVRLA A
	Isoform 3 [UniParc]. Checksum: 3C8467B533BAB019	FASTA	403	44,881
	10 20 30 40 50 60 MQGTPGGGTR PGPSPVDRRT LLVFSFILAA ALGQMNFTGD QVLRVLAKDE KQLSLLGDLE 70 80 90 100 110 120 GLKPQKVDFW RGPARPSLPV DMRVPFSELK DIKAYLESHG LAYSIMIKDI QVLLDEERQA 130 140 150 160 170 180 MAKSRRLERS TNSFSYSSYH TLEEIYSWID NFVMEHSDIV SKIQIGNSFE NQSILVLKFS 190 200 210 220 230 240 TGGSRHPAIW IDTGIHSREW ITHATGIWTA NKIVSDYGKD RVLTDILNAM DIFIELVTNP 250 260 270 280 290 300 DGFAFTHSMN RLWRKNKSIR PGIFCIGVDL NRNWKSGFGG NGSNSNPCSE TYHGPSPQSE 310 320 330 340 350 360 PEVAAIVNFI TAHGNFKALI SIHSYSQMLM YPYGRLLEPV SNQRELMWPV GSPSTGPMTV 370 380 390 400 ASSTPSALSS GTLGSMASCC RPHRSSPRPR RRGWRFGPSW STP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mutation screening and imprinting analysis of four candidate genes for autism in the 7q32 region." Bonora E., Bacchelli E., Levy E.R., Blasi F., Marlow A., Monaco A.P., Maestrini E. Mol. Psychiatry 7:289-301(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS SER-79; SER-336 AND ASP-338.
[2]	"The imprinted region on human chromosome 7q32 extends to the carboxypeptidase A gene cluster: an imprinted candidate for Silver-Russell syndrome." Bentley L., Nakabayashi K., Monk D., Beechey C., Peters J., Birjandi Z., Khayat F.E., Patel M., Preece M.A., Stanier P., Scherer S.W., Moore G.E. J. Med. Genet. 40:249-256(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Medulla oblongata.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Testis.
[4]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS SER-336 AND ASP-338. Tissue: Testis.
[7]	"Identification and characterization of three members of the human metallocarboxypeptidase gene family." Wei S., Segura S., Vendrell J., Aviles F.X., Lanoue E., Day R., Feng Y., Fricker L.D. J. Biol. Chem. 277:14954-14964(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE STRUCTURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF384667 mRNA. Translation: AAL37611.1. AY155229 mRNA. Translation: AAO17155.1. AY155230 mRNA. Translation: AAO17156.1. AK093288 mRNA. Translation: BAC04122.1. AK131191 mRNA. Translation: BAD18389.1. AC007938 Genomic DNA. No translation available. CH471070 Genomic DNA. Translation: EAW83757.1. BC039362 mRNA. Translation: AAH39362.1. BC042996 mRNA. Translation: AAH42996.1. BK000187 mRNA. Translation: DAA00035.1.
IPI	IPI00103586. IPI00384440.
RefSeq	NP_001120913.1. NM_001127441.1. NP_001120914.1. NM_001127442.1. NP_525124.3. NM_080385.4.
UniGene	Hs.567642.
3D structure databases
ProteinModelPortal	Q8WXQ8.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000347549.
Protein family/group databases
MEROPS	M14.020.
Polymorphism databases
DMDM	38257690.
Proteomic databases
PaxDb	Q8WXQ8.
PRIDE	Q8WXQ8.
Protocols and materials databases
DNASU	93979.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000355388; ENSP00000347549; ENSG00000158525. ENST00000393213; ENSP00000376907; ENSG00000158525. ENST00000431780; ENSP00000393045; ENSG00000158525. ENST00000461828; ENSP00000418183; ENSG00000158525. ENST00000466363; ENSP00000419025; ENSG00000158525. ENST00000474905; ENSP00000417314; ENSG00000158525. ENST00000485477; ENSP00000420237; ENSG00000158525. ENST00000579032; ENSP00000464444; ENSG00000266292. ENST00000579725; ENSP00000462245; ENSG00000266292. ENST00000582999; ENSP00000462551; ENSG00000266292. ENST00000583967; ENSP00000464290; ENSG00000266292. ENST00000583996; ENSP00000463370; ENSG00000266292. ENST00000584427; ENSP00000461972; ENSG00000266292. ENST00000584835; ENSP00000464329; ENSG00000266292.
GeneID	93979.
KEGG	hsa:93979.
UCSC	uc003vps.2. human. uc003vpt.2. human.
Organism-specific databases
CTD	93979.
GeneCards	GC07P129986.
HGNC	HGNC:15722. CPA5.
HPA	HPA020322.
MIM	609561. gene.
neXtProt	NX_Q8WXQ8.
PharmGKB	PA26820.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2866.
HOGENOM	HOG000252967.
HOVERGEN	HBG050815.
InParanoid	Q8WXQ8.
KO	K08781.
OMA	WRKNKSI.
OrthoDB	EOG45X7W4.
PhylomeDB	Q8WXQ8.
Gene expression databases
ArrayExpress	Q8WXQ8.
Bgee	Q8WXQ8.
CleanEx	HS_CPA5.
Genevestigator	Q8WXQ8.
GermOnline	ENSG00000158525. Homo sapiens.
Family and domain databases
Gene3D	3.30.70.340. 1 hit.
InterPro	IPR000834. Peptidase_M14. IPR003146. Prot_inh_M14A. IPR009020. Prot_inh_propept. [Graphical view]
Pfam	PF00246. Peptidase_M14. 1 hit. PF02244. Propep_M14. 1 hit. [Graphical view]
PRINTS	PR00765. CRBOXYPTASEA.
SMART	SM00631. Zn_pept. 1 hit. [Graphical view]
SUPFAM	SSF54897. Prot_inh_propept. 1 hit.
PROSITE	PS00132. CARBOXYPEPT_ZN_1. 1 hit. PS00133. CARBOXYPEPT_ZN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q8WXQ8.
ChEMBL	CHEMBL2234.
GenomeRNAi	93979.
NextBio	78246.
SOURCE	Search...

Entry information

Entry name

CBPA5_HUMAN

Accession

Primary (citable) accession number: Q8WXQ8
Secondary accession number(s): G3V0G8 , Q6ZNI6, Q86SE2, Q86XM3, Q8NA08

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	March 1, 2002
Last modified:	May 29, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.