Reviewed, UniProtKB/Swiss-Prot Q8WXQ8 (CBPA5_HUMAN)

Names and origin

Protein names

Recommended name:
    Carboxypeptidase A5
    EC=3.4.17.1

Gene names

Name:

CPA5

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Expression is very low or not detectable.
Sequence similarities	Belongs to the peptidase M14 family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Carboxypeptidase Hydrolase Metalloprotease Protease
PTM	Cleavage on pair of basic residues Disulfide bond Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metallocarboxypeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8WXQ8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8WXQ8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     347-351: YDLAK → VRLAA
     352-436: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Potential
	Propeptide	34 – 126	93	Activation peptide By similarity		PRO_0000004361
	Chain	127 – 436	310	Carboxypeptidase A5		PRO_0000004362
Regions
	Region	196 – 199	4	Substrate binding By similarity
	Region	271 – 272	2	Substrate binding By similarity
	Region	324 – 325	2	Substrate binding By similarity
Sites
	Active site	397	1	Nucleophile By similarity
	Metal binding	196	1	Zinc; catalytic By similarity
	Metal binding	199	1	Zinc; catalytic By similarity
	Metal binding	323	1	Zinc; catalytic By similarity
	Binding site	254	1	Substrate By similarity
Amino acid modifications
	Disulfide bond	265 ↔ 288		By similarity
Natural variations
	Alternative sequence	347 – 351	5	YDLAK → VRLAA in isoform 2.		VSP_008806
	Alternative sequence	352 – 436	85	Missing in isoform 2.		VSP_008807
	Natural variant	79	1	P → S: dbSNP rs17388190. Ref.1		VAR_017191
	Natural variant	336	1	L → S: dbSNP rs11761888. Ref.1 Ref.5		VAR_017192
	Natural variant	338	1	E → D		VAR_017193
	Natural variant	378	1	S → G: dbSNP rs11765961.		VAR_048596
Experimental info
	Sequence conflict	108	1	K → R in BAC04122. Ref.3
	Sequence conflict	117	1	E → G in AAH39362. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: A6EB4FFDE72F355A	FASTA	436	49,036
	10 20 30 40 50 60 MQGTPGGGTR PGPSPVDRRT LLVFSFILAA ALGQMNFTGD QVLRVLAKDE KQLSLLGDLE 70 80 90 100 110 120 GLKPQKVDFW RGPARPSLPV DMRVPFSELK DIKAYLESHG LAYSIMIKDI QVLLDEERQA 130 140 150 160 170 180 MAKSRRLERS TNSFSYSSYH TLEEIYSWID NFVMEHSDIV SKIQIGNSFE NQSILVLKFS 190 200 210 220 230 240 TGGSRHPAIW IDTGIHSREW ITHATGIWTA NKIVSDYGKD RVLTDILNAM DIFIELVTNP 250 260 270 280 290 300 DGFAFTHSMN RLWRKNKSIR PGIFCIGVDL NRNWKSGFGG NGSNSNPCSE TYHGPSPQSE 310 320 330 340 350 360 PEVAAIVNFI TAHGNFKALI SIHSYSQMLM YPYGRLLEPV SNQRELYDLA KDAVEALYKV 370 380 390 400 410 420 HGIEYIFGSI STTLYVASGI TVDWAYDSGI KYAFSFELRD TGQYGFLLPA TQIIPTAQET 430 WMALRTIMEH TLNHPY
	Isoform 2. Checksum: 9D083276FD2D6B16	FASTA	351	39,364
	10 20 30 40 50 60 MQGTPGGGTR PGPSPVDRRT LLVFSFILAA ALGQMNFTGD QVLRVLAKDE KQLSLLGDLE 70 80 90 100 110 120 GLKPQKVDFW RGPARPSLPV DMRVPFSELK DIKAYLESHG LAYSIMIKDI QVLLDEERQA 130 140 150 160 170 180 MAKSRRLERS TNSFSYSSYH TLEEIYSWID NFVMEHSDIV SKIQIGNSFE NQSILVLKFS 190 200 210 220 230 240 TGGSRHPAIW IDTGIHSREW ITHATGIWTA NKIVSDYGKD RVLTDILNAM DIFIELVTNP 250 260 270 280 290 300 DGFAFTHSMN RLWRKNKSIR PGIFCIGVDL NRNWKSGFGG NGSNSNPCSE TYHGPSPQSE 310 320 330 340 350 PEVAAIVNFI TAHGNFKALI SIHSYSQMLM YPYGRLLEPV SNQRELVRLA A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mutation screening and imprinting analysis of four candidate genes for autism in the 7q32 region." Bonora E., Bacchelli E., Levy E.R., Blasi F., Marlow A., Monaco A.P., Maestrini E. Mol. Psychiatry 7:289-301(2002) [PubMed: 11920156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS SER-79; SER-336 AND ASP-338.
[2]	"The imprinted region on human chromosome 7q32 extends to the carboxypeptidase A gene cluster: an imprinted candidate for Silver-Russell syndrome." Bentley L., Nakabayashi K., Monk D., Beechey C., Peters J., Birjandi Z., Khayat F.E., Patel M., Preece M.A., Stanier P., Scherer S.W., Moore G.E. J. Med. Genet. 40:249-256(2003) [PubMed: 12676894] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Medulla oblongata.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[4]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS SER-336 AND ASP-338. Tissue: Testis.
[6]	"Identification and characterization of three members of the human metallocarboxypeptidase gene family." Wei S., Segura S., Vendrell J., Aviles F.X., Lanoue E., Day R., Feng Y., Fricker L.D. J. Biol. Chem. 277:14954-14964(2002) [PubMed: 11836249] [Abstract] Cited for: GENE STRUCTURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF384667 mRNA. Translation: AAL37611.1. AY155229 mRNA. Translation: AAO17155.1. AY155230 mRNA. Translation: AAO17156.1. AK093288 mRNA. Translation: BAC04122.1. BC039362 mRNA. Translation: AAH39362.1. BC042996 mRNA. Translation: AAH42996.1. BK000187 mRNA. Translation: DAA00035.1.
IPI	IPI00103586. IPI00384440.
RefSeq	NP_001120913.1. NP_001120914.1. NP_525124.3.
UniGene	Hs.567642
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8WXQ8.
Protein family/group databases
MEROPS	M14.020.
Proteomic databases
PRIDE	Q8WXQ8.
Genome annotation databases
Ensembl	ENST00000355388; ENSP00000347549; ENSG00000158525; Homo sapiens. [Genome view] ENST00000393213; ENSP00000376907; ENSG00000158525; Homo sapiens. [Genome view] ENST00000461828; ENSP00000418183; ENSG00000158525; Homo sapiens. [Genome view] ENST00000466363; ENSP00000419025; ENSG00000158525; Homo sapiens. [Genome view] ENST00000474905; ENSP00000417314; ENSG00000158525; Homo sapiens. [Genome view] ENST00000485477; ENSP00000420237; ENSG00000158525; Homo sapiens. [Genome view]
GeneID	93979.
KEGG	hsa:93979.
UCSC	uc003vps.2. human. uc003vpt.2. human.
Organism-specific databases
CTD	93979.
GeneCards	GC07P129772.
H-InvDB	HIX0007075.
HGNC	HGNC:15722. CPA5.
MIM	609561. gene.
PharmGKB	PA26820.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q8WXQ8.
HOVERGEN	Q8WXQ8.
OMA	GSNSNPC
Enzyme and pathway databases
BRENDA	3.4.17.1. 247.
Gene expression databases
ArrayExpress	Q8WXQ8.
Bgee	Q8WXQ8.
CleanEx	HS_CPA5.
Genevestigator	Q8WXQ8.
GermOnline	ENSG00000158525. Homo sapiens.
Family and domain databases
InterPro	IPR000834. Peptidase_M14. IPR003146. Prot_inh_M14A. IPR009020. Prot_inh_propept. [Graphical view]
Pfam	PF00246. Peptidase_M14. 1 hit. PF02244. Propep_M14. 1 hit. [Graphical view]
PRINTS	PR00765. CRBOXYPTASEA.
SMART	SM00631. Zn_pept. 1 hit. [Graphical view]
PROSITE	PS00132. CARBOXYPEPT_ZN_1. 1 hit. PS00133. CARBOXYPEPT_ZN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	78246.
SOURCE	Search...

Entry information

Entry name

CBPA5_HUMAN

Accession

Primary (citable) accession number: Q8WXQ8
Secondary accession number(s): Q86SE2, Q86XM3, Q8NA08

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	March 1, 2002
Last modified:	November 24, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.