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Protein

Acyl-coenzyme A thioesterase 11

Gene

ACOT11

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei181Coenzyme ABy similarity1

GO - Molecular functioni

  • acyl-CoA hydrolase activity Source: UniProtKB
  • carboxylic ester hydrolase activity Source: UniProtKB-KW
  • lipid binding Source: InterPro

GO - Biological processi

  • acyl-CoA metabolic process Source: GO_Central
  • fatty acid metabolic process Source: UniProtKB
  • intracellular signal transduction Source: UniProtKB
  • long-chain fatty-acyl-CoA biosynthetic process Source: Reactome
  • response to cold Source: BHF-UCL
  • response to temperature stimulus Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

BioCyciZFISH:ENSG00000162390-MONOMER.
ReactomeiR-HSA-75105. Fatty Acyl-CoA Biosynthesis.

Chemistry databases

SwissLipidsiSLP:000001186. [Q8WXI4-2]

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A thioesterase 11 (EC:3.1.2.-)
Short name:
Acyl-CoA thioesterase 11
Alternative name(s):
Acyl-CoA thioester hydrolase 11
Adipose-associated thioesterase
Brown fat-inducible thioesterase
Short name:
BFIT
Gene namesi
Name:ACOT11
Synonyms:BFIT, KIAA0707, THEA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:18156. ACOT11.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: GO_Central
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi26027.
OpenTargetsiENSG00000162390.
PharmGKBiPA38303.

Polymorphism and mutation databases

BioMutaiACOT11.
DMDMi21363000.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538131 – 607Acyl-coenzyme A thioesterase 11Add BLAST607

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineBy similarity1
Modified residuei25PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8WXI4.
PaxDbiQ8WXI4.
PeptideAtlasiQ8WXI4.
PRIDEiQ8WXI4.

PTM databases

iPTMnetiQ8WXI4.
PhosphoSitePlusiQ8WXI4.

Expressioni

Tissue specificityi

Isoform 1 is predominantly expressed in skeletal muscle, liver, testis, stomach, spleen, lung and brain. Isoform 2 is predominantly expressed in kidney, uterus, hibernoma and white adipose tissue.

Inductioni

By cold exposure and repressed by heat exposure.

Gene expression databases

BgeeiENSG00000162390.
CleanExiHS_ACOT11.
GenevisibleiQ8WXI4. HS.

Organism-specific databases

HPAiHPA035309.

Interactioni

Protein-protein interaction databases

BioGridi117495. 4 interactors.
STRINGi9606.ENSP00000360366.

Structurei

Secondary structure

1607
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi347 – 363Combined sources17
Turni370 – 372Combined sources3
Beta strandi375 – 378Combined sources4
Helixi381 – 383Combined sources3
Helixi384 – 399Combined sources16
Beta strandi405 – 410Combined sources6
Beta strandi413 – 419Combined sources7
Beta strandi424 – 434Combined sources11
Helixi436 – 444Combined sources9
Helixi446 – 451Combined sources6
Beta strandi458 – 466Combined sources9
Beta strandi469 – 476Combined sources8
Beta strandi486 – 495Combined sources10
Beta strandi504 – 512Combined sources9
Beta strandi522 – 524Combined sources3
Beta strandi528 – 538Combined sources11
Beta strandi541 – 543Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FO5X-ray2.00A/B339-543[»]
ProteinModelPortaliQ8WXI4.
SMRiQ8WXI4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8WXI4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 155HotDog ACOT-type 1PROSITE-ProRule annotationAdd BLAST113
Domaini216 – 329HotDog ACOT-type 2PROSITE-ProRule annotationAdd BLAST114
Domaini375 – 585STARTPROSITE-ProRule annotationAdd BLAST211

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni91 – 93Coenzyme A bindingBy similarity3
Regioni120 – 122Coenzyme A bindingBy similarity3
Regioni271 – 273Coenzyme A bindingBy similarity3

Sequence similaritiesi

Contains 2 HotDog ACOT-type domains.PROSITE-ProRule annotation
Contains 1 START domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2763. Eukaryota.
COG1607. LUCA.
GeneTreeiENSGT00760000119297.
HOVERGENiHBG023847.
InParanoidiQ8WXI4.
KOiK12417.
OMAiQLTKVSY.
OrthoDBiEOG091G06LZ.
PhylomeDBiQ8WXI4.
TreeFamiTF328368.

Family and domain databases

Gene3Di3.10.129.10. 2 hits.
3.30.530.20. 1 hit.
InterProiIPR033120. HOTDOG_ACOT.
IPR029069. HotDog_dom.
IPR023393. START-like_dom.
IPR002913. START_lipid-bd_dom.
IPR006683. Thioestr_dom.
[Graphical view]
PfamiPF03061. 4HBT. 2 hits.
PF01852. START. 1 hit.
[Graphical view]
SMARTiSM00234. START. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 2 hits.
PROSITEiPS51770. HOTDOG_ACOT. 2 hits.
PS50848. START. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WXI4-1) [UniParc]FASTAAdd to basket
Also known as: BFIT1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIQNVGNHLR RGLASVFSNR TSRKSALRAG NDSAMADGEG YRNPTEVQMS
60 70 80 90 100
QLVLPCHTNQ RGELSVGQLL KWIDTTACLS AERHAGCPCV TASMDDIYFE
110 120 130 140 150
HTISVGQVVN IKAKVNRAFN SSMEVGIQVA SEDLCSEKQW NVCKALATFV
160 170 180 190 200
ARREITKVKL KQITPRTEEE KMEHSVAAER RRMRLVYADT IKDLLANCAI
210 220 230 240 250
QGDLESRDCS RMVPAEKTRV ESVELVLPPH ANHQGNTFGG QIMAWMENVA
260 270 280 290 300
TIAASRLCRA HPTLKAIEMF HFRGPSQVGD RLVLKAIVNN AFKHSMEVGV
310 320 330 340 350
CVEAYRQEAE THRRHINSAF MTFVVLDADD QPQLLPWIRP QPGDGERRYR
360 370 380 390 400
EASARKKIRL DRKYIVSCKQ TEVPLSVPWD PSNQVYLSYN NVSSLKMLVA
410 420 430 440 450
KDNWVLSSEI SQVRLYTLED DKFLSFHMEM VVHVDAAQAF LLLSDLRQRP
460 470 480 490 500
EWDKHYRSVE LVQQVDEDDA IYHVTSPALG GHTKPQDFVI LASRRKPCDN
510 520 530 540 550
GDPYVIALRS VTLPTHRETP EYRRGETLCS GFCLWREGDQ LTKCCWVRVS
560 570 580 590 600
LTELVSASGF YSWGLESRSK GRRSDGWNGK LAGGHLSTLK AIPVAKINSR

FGYLQDT
Length:607
Mass (Da):68,492
Last modified:March 1, 2002 - v1
Checksum:i12F2BCAB8AAC18EC
GO
Isoform 2 (identifier: Q8WXI4-2) [UniParc]FASTAAdd to basket
Also known as: BFIT2

The sequence of this isoform differs from the canonical sequence as follows:
     544-607: CCWVRVSLTE...NSRFGYLQDT → VSYYNQATPG...NDLAPSLQTL

Show »
Length:594
Mass (Da):67,152
Checksum:iC9CD42FB285A8B53
GO

Sequence cautioni

The sequence BAA31682 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti255S → R in AAH01517 (PubMed:15489334).Curated1
Sequence conflicti348R → W in BAD97290 (Ref. 4) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04819011R → W.Corresponds to variant rs34630746dbSNPEnsembl.1
Natural variantiVAR_022119165P → L.Corresponds to variant rs2304306dbSNPEnsembl.1
Natural variantiVAR_022120202G → D.1 PublicationCorresponds to variant rs1702003dbSNPEnsembl.1
Natural variantiVAR_022121212M → I.Corresponds to variant rs2304305dbSNPEnsembl.1
Natural variantiVAR_048191536R → H.Corresponds to variant rs12403630dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000160544 – 607CCWVR…YLQDT → VSYYNQATPGVLNYVTTNVA GLSSEFYTTFKACEQFLLDN RNDLAPSLQTL in isoform 2. 4 PublicationsAdd BLAST64

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF416921 mRNA. Translation: AAL40937.1.
AF416922 mRNA. Translation: AAL40938.1.
AB014607 mRNA. Translation: BAA31682.1. Different initiation.
AK023937 mRNA. Translation: BAB14734.1.
AK223570 mRNA. Translation: BAD97290.1.
AL590093, AC099796 Genomic DNA. Translation: CAH71611.1.
AL590093, AC099796 Genomic DNA. Translation: CAH71612.1.
CH471059 Genomic DNA. Translation: EAX06682.1.
CH471059 Genomic DNA. Translation: EAX06683.1.
CH471059 Genomic DNA. Translation: EAX06684.1.
BC001517 mRNA. Translation: AAH01517.1.
BC093844 mRNA. Translation: AAH93844.1.
BC093846 mRNA. Translation: AAH93846.1.
CCDSiCCDS592.1. [Q8WXI4-1]
CCDS593.1. [Q8WXI4-2]
PIRiT00351.
RefSeqiNP_056362.1. NM_015547.3. [Q8WXI4-1]
NP_671517.1. NM_147161.3. [Q8WXI4-2]
UniGeneiHs.745173.

Genome annotation databases

EnsembliENST00000343744; ENSP00000340260; ENSG00000162390. [Q8WXI4-2]
ENST00000371316; ENSP00000360366; ENSG00000162390. [Q8WXI4-1]
GeneIDi26027.
KEGGihsa:26027.
UCSCiuc001cxl.3. human. [Q8WXI4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF416921 mRNA. Translation: AAL40937.1.
AF416922 mRNA. Translation: AAL40938.1.
AB014607 mRNA. Translation: BAA31682.1. Different initiation.
AK023937 mRNA. Translation: BAB14734.1.
AK223570 mRNA. Translation: BAD97290.1.
AL590093, AC099796 Genomic DNA. Translation: CAH71611.1.
AL590093, AC099796 Genomic DNA. Translation: CAH71612.1.
CH471059 Genomic DNA. Translation: EAX06682.1.
CH471059 Genomic DNA. Translation: EAX06683.1.
CH471059 Genomic DNA. Translation: EAX06684.1.
BC001517 mRNA. Translation: AAH01517.1.
BC093844 mRNA. Translation: AAH93844.1.
BC093846 mRNA. Translation: AAH93846.1.
CCDSiCCDS592.1. [Q8WXI4-1]
CCDS593.1. [Q8WXI4-2]
PIRiT00351.
RefSeqiNP_056362.1. NM_015547.3. [Q8WXI4-1]
NP_671517.1. NM_147161.3. [Q8WXI4-2]
UniGeneiHs.745173.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FO5X-ray2.00A/B339-543[»]
ProteinModelPortaliQ8WXI4.
SMRiQ8WXI4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117495. 4 interactors.
STRINGi9606.ENSP00000360366.

Chemistry databases

SwissLipidsiSLP:000001186. [Q8WXI4-2]

PTM databases

iPTMnetiQ8WXI4.
PhosphoSitePlusiQ8WXI4.

Polymorphism and mutation databases

BioMutaiACOT11.
DMDMi21363000.

Proteomic databases

MaxQBiQ8WXI4.
PaxDbiQ8WXI4.
PeptideAtlasiQ8WXI4.
PRIDEiQ8WXI4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000343744; ENSP00000340260; ENSG00000162390. [Q8WXI4-2]
ENST00000371316; ENSP00000360366; ENSG00000162390. [Q8WXI4-1]
GeneIDi26027.
KEGGihsa:26027.
UCSCiuc001cxl.3. human. [Q8WXI4-1]

Organism-specific databases

CTDi26027.
DisGeNETi26027.
GeneCardsiACOT11.
HGNCiHGNC:18156. ACOT11.
HPAiHPA035309.
MIMi606803. gene.
neXtProtiNX_Q8WXI4.
OpenTargetsiENSG00000162390.
PharmGKBiPA38303.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2763. Eukaryota.
COG1607. LUCA.
GeneTreeiENSGT00760000119297.
HOVERGENiHBG023847.
InParanoidiQ8WXI4.
KOiK12417.
OMAiQLTKVSY.
OrthoDBiEOG091G06LZ.
PhylomeDBiQ8WXI4.
TreeFamiTF328368.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000162390-MONOMER.
ReactomeiR-HSA-75105. Fatty Acyl-CoA Biosynthesis.

Miscellaneous databases

EvolutionaryTraceiQ8WXI4.
GeneWikiiACOT11.
GenomeRNAii26027.
PROiQ8WXI4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000162390.
CleanExiHS_ACOT11.
GenevisibleiQ8WXI4. HS.

Family and domain databases

Gene3Di3.10.129.10. 2 hits.
3.30.530.20. 1 hit.
InterProiIPR033120. HOTDOG_ACOT.
IPR029069. HotDog_dom.
IPR023393. START-like_dom.
IPR002913. START_lipid-bd_dom.
IPR006683. Thioestr_dom.
[Graphical view]
PfamiPF03061. 4HBT. 2 hits.
PF01852. START. 1 hit.
[Graphical view]
SMARTiSM00234. START. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 2 hits.
PROSITEiPS51770. HOTDOG_ACOT. 2 hits.
PS50848. START. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACO11_HUMAN
AccessioniPrimary (citable) accession number: Q8WXI4
Secondary accession number(s): B1AQ22
, D3DQ50, O75187, Q52LP1, Q53ER9, Q96DI1, Q9H883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.