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Reviewed, UniProtKB/Swiss-Prot Q8WXI2 (CNKR2_HUMAN)

Last modified March 2, 2010. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
Connector enhancer of kinase suppressor of ras 2
Short name=Connector enhancer of KSR 2
Alternative name(s):
CNK homolog protein 2
Short name=CNK2
Gene names
Name:CNKSR2
Synonyms:CNK2, KIAA0902, KSR2
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length1034 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

May function as an adapter protein or regulator of Ras signaling pathways. Ref.1

Subunit structure

Interacts with RAF1, RAB2L and RAL GTPase proteins. Ref.1

Subcellular location

Cytoplasm. Membrane; Peripheral membrane protein.

Post-translational modification

Phosphorylated on tyrosine. Ref.1 Ref.6

Sequence similarities

Belongs to the CNKSR family.

Contains 1 CRIC domain.

Contains 1 DUF1170 domain.

Contains 1 PDZ (DHR) domain.

Contains 1 PH domain.

Contains 1 SAM (sterile alpha motif) domain.

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Ontologies

Keywords
   Cellular componentCytoplasm
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainCoiled coil
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processregulation of signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular componentcentrosome

Inferred from direct assay. Source: HPA

plasma membrane

Inferred from direct assay. Source: HPA

   Molecular functionprotein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

MOBKL2BQ86TA11EBI-1045119,EBI-751703

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8WXI2-1)

Also known as: CNK2A; KSR2A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8WXI2-2)

Also known as: CNK2B; KSR2B;

The sequence of this isoform differs from the canonical sequence as follows:
     899-1034: Missing.
Note: Ref.2 (BAA74925) sequence differs from that shown due to frameshifts in positions 859, 929, 985 and 1019.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10341034Connector enhancer of kinase suppressor of ras 2
PRO_0000089970

Regions

Domain11 – 7666SAM
Domain84 – 17895CRIC
Domain215 – 29783PDZ
Domain332 – 515184DUF1170
Domain570 – 669100PH
Coiled coil875 – 90430 Potential
Compositional bias354 – 3574Poly-Pro
Compositional bias703 – 7064Poly-Pro
Compositional bias875 – 88814Poly-Glu

Amino acid modifications

Modified residue2481Phosphoserine By similarity
Modified residue3251Phosphoserine By similarity
Modified residue3901Phosphoserine By similarity
Modified residue4671Phosphoserine By similarity
Modified residue8461Phosphothreonine By similarity
Modified residue9081Phosphoserine Ref.6

Natural variations

Alternative sequence899 – 1034136Missing in isoform 2.
VSP_010887
Natural variant461R → H in a colorectal cancer sample; somatic mutation. Ref.8
VAR_035681

Secondary structure

1 .............. 1034
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (CNK2A) (KSR2A) [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: E43DB0D8D72D954C

FASTA1,034117,535
        10         20         30         40         50         60 
MALIMEPVSK WSPSQVVDWM KGLDDCLQQY IKNFEREKIS GDQLLRITHQ ELEDLGVSRI 

        70         80         90        100        110        120 
GHQELILEAV DLLCALNYGL ETENLKTLSH KLNASAKNLQ NFITGRRRSG HYDGRTSRKL 

       130        140        150        160        170        180 
PNDFLTSVVD LIGAAKSLLA WLDRSPFAAV TDYSVTRNNV IQLCLELTTI VQQDCTVYET 

       190        200        210        220        230        240 
ENKILHVCKT LSGVCDHIIS LSSDPLVSQS AHLEVIQLAN IKPSEGLGMY IKSTYDGLHV 

       250        260        270        280        290        300 
ITGTTENSPA DRCKKIHAGD EVIQVNHQTV VGWQLKNLVN ALREDPSGVI LTLKKRPQSM 

       310        320        330        340        350        360 
LTSAPALLKN MRWKPLALQP LIPRSPTSSV ATPSSTISTP TKRDSSALQD LYIPPPPAEP 

       370        380        390        400        410        420 
YIPRDEKGNL PCEDLRGHMV GKPVHKGSES PNSFLDQEYR KRFNIVEEDT VLYCYEYEKG 

       430        440        450        460        470        480 
RSSSQGRRES TPTYGKLRPI SMPVEYNWVG DYEDPNKMKR DSRRENSLLR YMSNEKIAQE 

       490        500        510        520        530        540 
EYMFQRNSKK DTGKKSKKKG DKSNSPTHYS LLPSLQMDAL RQDIMGTPVP ETTLYHTFQQ 

       550        560        570        580        590        600 
SSLQHKSKKK NKGPIAGKSK RRISCKDLGR GDCEGWLWKK KDAKSYFSQK WKKYWFVLKD 

       610        620        630        640        650        660 
ASLYWYINEE DEKAEGFISL PEFKIDRASE CRKKYAFKAC HPKIKSFYFA AEHLDDMNRW 

       670        680        690        700        710        720 
LNRINMLTAG YAERERIKQE QDYWSESDKE EADTPSTPKQ DSPPPPYDTY PRPPSMSCAS 

       730        740        750        760        770        780 
PYVEAKHSRL SSTETSQSQS SHEEFRQEVT GSSAVSPIRK TASQRRSWQD LIETPLTSSG 

       790        800        810        820        830        840 
LHYLQTLPLE DSVFSDSAAI SPEHRRQSTL PTQKCHLQDH YGPYPLAESE RMQVLNGNGG 

       850        860        870        880        890        900 
KPRSFTLPRD SGFNHCCLNA PVSACDPQDD VQPPEVEEEE EEEEEEGEAA GENIGEKSES 

       910        920        930        940        950        960 
REEKLGDSLQ DLYRALEQAS LSPLGEHRIS TKMEYKLSFI KRCNDPVMNE KLHRLRILKS 

       970        980        990       1000       1010       1020 
TLKAREGEVA IIDKVLDNPD LTSKEFQQWK QMYLDLFLDI CQNTTSNDPL SISSEVDVIT 

      1030 
SSLAHTHSYI ETHV

« Hide

Isoform 2 (CNK2B) (KSR2B).

Checksum: 4DFEFE26960DF70C
Show »

FASTA898101,874

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References

« Hide 'large scale' references
[1]"Human homologue of Drosophila CNK interacts with Ras effector proteins Raf and Rlf."
Lanigan T.M., Liu A., Huang Y.Z., Mei L., Margolis B., Guan K.-L.
FASEB J. 17:2048-2060(2003) [PubMed: 14597674] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, INTERACTION WITH RAF1; RAB2L AND RAL GTPASE PROTEINS, PHOSPHORYLATION.
Tissue: Fetal brain.
[2]"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:355-364(1998) [PubMed: 10048485] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[3]"The DNA sequence of the human X chromosome."
Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. expand/collapse author list , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
Nature 434:325-337(2005) [PubMed: 15772651] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain and Testis.
[6]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-908, MASS SPECTROMETRY.
Tissue: T-cell.
[7]"Solution structure of the N-terminal SAM-domain of human KIAA0902 protein (connector enhancer of kinase suppressor of RAS 2)."
RIKEN structural genomics initiative (RSGI)
Submitted (JUL-2007) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 8-79.
[8]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed: 16959974] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] HIS-46.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF418269 mRNA. Translation: AAL60502.1.
AF418270 mRNA. Translation: AAL60503.1.
AB020709 mRNA. Translation: BAA74925.2. Frameshift.
AL928874, AL772392, AL807781 Genomic DNA. Translation: CAH70863.1.
AL928874, AL772392, AL807781 Genomic DNA. Translation: CAH70864.1.
AL807781, AL772392, AL928874 Genomic DNA. Translation: CAI39866.1.
AL807781, AL772392, AL928874 Genomic DNA. Translation: CAI39867.1.
AL772392, AL807781, AL928874 Genomic DNA. Translation: CAI40765.1.
AL772392, AL807781, AL928874 Genomic DNA. Translation: CAI40766.1.
CH471074 Genomic DNA. Translation: EAW98977.1.
BC126121 mRNA. Translation: AAI26122.1.
BC136289 mRNA. Translation: AAI36290.1.
IPIIPI00435208.
IPI00435209.
RefSeqNP_001162119.1.
NP_055742.2.
UniGeneHs.555917

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2EANNMR-A8-77[»]
3BS5X-ray2.00B5-84[»]
SMRQ8WXI2. Positions 200-304, 562-669.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-29736N.
IntActQ8WXI2. 5 interactions.
STRINGQ8WXI2.

PTM databases

PhosphoSiteQ8WXI2.

Proteomic databases

PRIDEQ8WXI2.

Genome annotation databases

EnsemblENST00000379510; ENSP00000368824; ENSG00000149970; Homo sapiens. [Genome view]
GeneID22866.
KEGGhsa:22866.
UCSCuc004czw.1. human.
uc004czx.1. human.

Organism-specific databases

CTD22866.
GeneCardsGC0XP021302.
H-InvDBHIX0020793.
HGNCHGNC:19701. CNKSR2.
HPAHPA001502.
PharmGKBPA134867759.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG17949.
HOGENOMHBG403049.
HOVERGENHBG051040.
InParanoidQ8WXI2.
OMAGHYDGRT.
PhylomeDBQ8WXI2.

Gene expression databases

ArrayExpressQ8WXI2.
BgeeQ8WXI2.
CleanExHS_CNKSR2.
HS_KSR2.
GenevestigatorQ8WXI2.
GermOnlineENSG00000149970. Homo sapiens.

Family and domain databases

InterProIPR010599. CNKSR2.
IPR017874. CRIC_domain.
IPR019555. CRIC_domain_Chordata.
IPR001478. PDZ/DHR/GLGF.
IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
IPR001660. SAM.
IPR010993. SAM_homology.
IPR013761. SAM_type.
IPR021129. SAM_type1.
[Graphical view]
Gene3DG3DSA:2.30.29.30. PH_type. 1 hit.
G3DSA:1.10.150.50. SAM_type. 1 hit.
PfamPF10534. CRIC_ras_sig. 1 hit.
PF06663. DUF1170. 1 hit.
PF00595. PDZ. 1 hit.
PF00169. PH. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTSM00228. PDZ. 1 hit.
SM00233. PH. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMSSF50156. PDZ. 1 hit.
SSF47769. SAM_homology. 1 hit.
PROSITEPS51290. CRIC. 1 hit.
PS50106. PDZ. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio43387.

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Entry information

Entry nameCNKR2_HUMAN
AccessionPrimary (citable) accession number: Q8WXI2
Secondary accession number(s): B9EG83 expand/collapse secondary AC list , O94976, Q5JPK4, Q5JPN0, Q8WXI1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: March 1, 2002
Last modified: March 2, 2010
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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PDB cross-references

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SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents