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Protein

Connector enhancer of kinase suppressor of ras 2

Gene

CNKSR2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May function as an adapter protein or regulator of Ras signaling pathways.1 Publication

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-5674135 MAP2K and MAPK activation
R-HSA-6802946 Signaling by moderate kinase activity BRAF mutants
R-HSA-6802948 Signaling by high-kinase activity BRAF mutants
R-HSA-6802949 Signaling by RAS mutants
R-HSA-6802952 Signaling by BRAF and RAF fusions
R-HSA-6802955 Paradoxical activation of RAF signaling by kinase inactive BRAF
SignaLinkiQ8WXI2
SIGNORiQ8WXI2

Names & Taxonomyi

Protein namesi
Recommended name:
Connector enhancer of kinase suppressor of ras 2
Short name:
Connector enhancer of KSR 2
Alternative name(s):
CNK homolog protein 2
Short name:
CNK2
Gene namesi
Name:CNKSR2
Synonyms:CNK2, KIAA0902, KSR2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000149970.14
HGNCiHGNC:19701 CNKSR2
MIMi300724 gene
neXtProtiNX_Q8WXI2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi22866
MalaCardsiCNKSR2
OpenTargetsiENSG00000149970
PharmGKBiPA134867759

Polymorphism and mutation databases

BioMutaiCNKSR2
DMDMi50400586

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000899701 – 1034Connector enhancer of kinase suppressor of ras 2Add BLAST1034

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineBy similarity1
Modified residuei338PhosphoserineBy similarity1
Modified residuei390PhosphoserineBy similarity1
Modified residuei683PhosphotyrosineBy similarity1
Modified residuei685PhosphoserineBy similarity1
Modified residuei687PhosphoserineBy similarity1
Modified residuei756PhosphoserineBy similarity1
Modified residuei767PhosphoserineBy similarity1
Modified residuei908PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on tyrosine.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8WXI2
MaxQBiQ8WXI2
PaxDbiQ8WXI2
PeptideAtlasiQ8WXI2
PRIDEiQ8WXI2

PTM databases

iPTMnetiQ8WXI2
PhosphoSitePlusiQ8WXI2

Expressioni

Gene expression databases

BgeeiENSG00000149970
CleanExiHS_CNKSR2
HS_KSR2
GenevisibleiQ8WXI2 HS

Organism-specific databases

HPAiHPA001502

Interactioni

Subunit structurei

Interacts with RAF1, RAB2L and RAL GTPase proteins.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
aveQ8ML925EBI-1045119,EBI-2563975From Drosophila melanogaster.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116534, 7 interactors
DIPiDIP-29736N
IntActiQ8WXI2, 4 interactors
MINTiQ8WXI2
STRINGi9606.ENSP00000368824

Structurei

Secondary structure

11034
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 10Combined sources3
Helixi13 – 21Combined sources9
Helixi25 – 30Combined sources6
Helixi31 – 37Combined sources7
Helixi41 – 45Combined sources5
Helixi49 – 54Combined sources6
Helixi60 – 77Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EANNMR-A8-77[»]
3BS5X-ray2.00B5-84[»]
ProteinModelPortaliQ8WXI2
SMRiQ8WXI2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8WXI2

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 76SAMPROSITE-ProRule annotationAdd BLAST66
Domaini84 – 178CRICPROSITE-ProRule annotationAdd BLAST95
Domaini215 – 297PDZPROSITE-ProRule annotationAdd BLAST83
Domaini332 – 515DUF1170Add BLAST184
Domaini570 – 669PHPROSITE-ProRule annotationAdd BLAST100

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili875 – 904Sequence analysisAdd BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi354 – 357Poly-Pro4
Compositional biasi703 – 706Poly-Pro4
Compositional biasi875 – 888Poly-GluAdd BLAST14

Sequence similaritiesi

Belongs to the CNKSR family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1738 Eukaryota
ENOG4110T89 LUCA
GeneTreeiENSGT00390000017199
HOGENOMiHOG000231501
HOVERGENiHBG051040
InParanoidiQ8WXI2
KOiK17536
OMAiSTKLEYK
OrthoDBiEOG091G037J
PhylomeDBiQ8WXI2
TreeFamiTF326495

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR010599 CNKSR2
IPR017874 CRIC_domain
IPR001478 PDZ
IPR036034 PDZ_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR001660 SAM
IPR013761 SAM/pointed_sf
PfamiView protein in Pfam
PF10534 CRIC_ras_sig, 1 hit
PF06663 DUF1170, 1 hit
PF00595 PDZ, 1 hit
PF00169 PH, 1 hit
PF00536 SAM_1, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SM00233 PH, 1 hit
SM00454 SAM, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit
SSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS51290 CRIC, 1 hit
PS50106 PDZ, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS50105 SAM_DOMAIN, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WXI2-1) [UniParc]FASTAAdd to basket
Also known as: CNK2A, KSR2A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALIMEPVSK WSPSQVVDWM KGLDDCLQQY IKNFEREKIS GDQLLRITHQ
60 70 80 90 100
ELEDLGVSRI GHQELILEAV DLLCALNYGL ETENLKTLSH KLNASAKNLQ
110 120 130 140 150
NFITGRRRSG HYDGRTSRKL PNDFLTSVVD LIGAAKSLLA WLDRSPFAAV
160 170 180 190 200
TDYSVTRNNV IQLCLELTTI VQQDCTVYET ENKILHVCKT LSGVCDHIIS
210 220 230 240 250
LSSDPLVSQS AHLEVIQLAN IKPSEGLGMY IKSTYDGLHV ITGTTENSPA
260 270 280 290 300
DRCKKIHAGD EVIQVNHQTV VGWQLKNLVN ALREDPSGVI LTLKKRPQSM
310 320 330 340 350
LTSAPALLKN MRWKPLALQP LIPRSPTSSV ATPSSTISTP TKRDSSALQD
360 370 380 390 400
LYIPPPPAEP YIPRDEKGNL PCEDLRGHMV GKPVHKGSES PNSFLDQEYR
410 420 430 440 450
KRFNIVEEDT VLYCYEYEKG RSSSQGRRES TPTYGKLRPI SMPVEYNWVG
460 470 480 490 500
DYEDPNKMKR DSRRENSLLR YMSNEKIAQE EYMFQRNSKK DTGKKSKKKG
510 520 530 540 550
DKSNSPTHYS LLPSLQMDAL RQDIMGTPVP ETTLYHTFQQ SSLQHKSKKK
560 570 580 590 600
NKGPIAGKSK RRISCKDLGR GDCEGWLWKK KDAKSYFSQK WKKYWFVLKD
610 620 630 640 650
ASLYWYINEE DEKAEGFISL PEFKIDRASE CRKKYAFKAC HPKIKSFYFA
660 670 680 690 700
AEHLDDMNRW LNRINMLTAG YAERERIKQE QDYWSESDKE EADTPSTPKQ
710 720 730 740 750
DSPPPPYDTY PRPPSMSCAS PYVEAKHSRL SSTETSQSQS SHEEFRQEVT
760 770 780 790 800
GSSAVSPIRK TASQRRSWQD LIETPLTSSG LHYLQTLPLE DSVFSDSAAI
810 820 830 840 850
SPEHRRQSTL PTQKCHLQDH YGPYPLAESE RMQVLNGNGG KPRSFTLPRD
860 870 880 890 900
SGFNHCCLNA PVSACDPQDD VQPPEVEEEE EEEEEEGEAA GENIGEKSES
910 920 930 940 950
REEKLGDSLQ DLYRALEQAS LSPLGEHRIS TKMEYKLSFI KRCNDPVMNE
960 970 980 990 1000
KLHRLRILKS TLKAREGEVA IIDKVLDNPD LTSKEFQQWK QMYLDLFLDI
1010 1020 1030
CQNTTSNDPL SISSEVDVIT SSLAHTHSYI ETHV
Length:1,034
Mass (Da):117,535
Last modified:March 1, 2002 - v1
Checksum:iE43DB0D8D72D954C
GO
Isoform 2 (identifier: Q8WXI2-2) [UniParc]FASTAAdd to basket
Also known as: CNK2B, KSR2B

The sequence of this isoform differs from the canonical sequence as follows:
     899-1034: Missing.

Show »
Length:898
Mass (Da):101,874
Checksum:i4DFEFE26960DF70C
GO
Isoform 3 (identifier: Q8WXI2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     271-319: Missing.
     899-1034: Missing.

Note: No experimental confirmation available.
Show »
Length:849
Mass (Da):96,366
Checksum:iD95B2A7AC2639040
GO
Isoform 4 (identifier: Q8WXI2-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     435-464: Missing.

Note: No experimental confirmation available.
Show »
Length:1,004
Mass (Da):113,914
Checksum:iC635EA09FA4E6696
GO

Sequence cautioni

Isoform 2 : The sequence BAA74925 differs from that shown. Reason: Frameshift at positions 859, 929, 985 and 1019.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03568146R → H in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs771705122Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_043168271 – 319Missing in isoform 3. 1 PublicationAdd BLAST49
Alternative sequenceiVSP_043169435 – 464Missing in isoform 4. 1 PublicationAdd BLAST30
Alternative sequenceiVSP_010887899 – 1034Missing in isoform 2 and isoform 3. 3 PublicationsAdd BLAST136

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF418269 mRNA Translation: AAL60502.1
AF418270 mRNA Translation: AAL60503.1
AB020709 mRNA Translation: BAA74925.2 Frameshift.
AK294728 mRNA Translation: BAG57875.1
AL928874 Genomic DNA No translation available.
AL807781 Genomic DNA No translation available.
AL772392 Genomic DNA No translation available.
CH471074 Genomic DNA Translation: EAW98977.1
BC126121 mRNA Translation: AAI26122.1
BC136289 mRNA Translation: AAI36290.1
BC143839 mRNA Translation: AAI43840.1
CCDSiCCDS14198.1 [Q8WXI2-1]
CCDS55387.1 [Q8WXI2-2]
CCDS55388.1 [Q8WXI2-5]
CCDS55389.1 [Q8WXI2-4]
RefSeqiNP_001162118.1, NM_001168647.2 [Q8WXI2-5]
NP_001162119.1, NM_001168648.2 [Q8WXI2-2]
NP_001162120.1, NM_001168649.2 [Q8WXI2-4]
NP_055742.2, NM_014927.4 [Q8WXI2-1]
UniGeneiHs.555917

Genome annotation databases

EnsembliENST00000279451; ENSP00000279451; ENSG00000149970 [Q8WXI2-2]
ENST00000379510; ENSP00000368824; ENSG00000149970 [Q8WXI2-1]
ENST00000425654; ENSP00000397906; ENSG00000149970 [Q8WXI2-5]
ENST00000543067; ENSP00000444633; ENSG00000149970 [Q8WXI2-4]
GeneIDi22866
KEGGihsa:22866
UCSCiuc004czw.4 human [Q8WXI2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCNKR2_HUMAN
AccessioniPrimary (citable) accession number: Q8WXI2
Secondary accession number(s): B4DGR4
, B7ZLJ1, B9EG83, E7ESA4, O94976, Q5JPK4, Q5JPN0, Q8WXI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: March 1, 2002
Last modified: May 23, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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