Q8WXG6 (MADD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MAP kinase-activating death domain protein

Alternative name(s):
Differentially expressed in normal and neoplastic cells
Insulinoma glucagonoma clone 20
Rab3 GDP/GTP exchange factor

Gene names

Name:	MADD
Synonyms:	DENN, IG20, KIAA0358

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1647 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect. Ref.2 Ref.4 Ref.10 Ref.11 Ref.12 Ref.17
Subunit structure	Interacts with the death domain of TNFRSF1A through its own death domain. Ref.2 Ref.4
Subcellular location	Membrane. Cytoplasm Ref.1.
Tissue specificity	Highly expressed in fetal brain and kidney; adult testis, ovary, brain and heart. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines. Ref.1 Ref.3 Ref.11
Miscellaneous	Overexpression of MADD activates the mitogen-activated protein (MAP) kinase extracellular signal-regulated kinase (ERK). Expression of the MADD death domain stimulates both the ERK and c-JUN N-terminal kinase MAP kinases and induces the phosphorylation of cytosolic phospholipase A2. Ref.2
Sequence similarities	Belongs to the MADD family. Contains 1 dDENN domain. Contains 1 death domain. Contains 1 DENN domain. Contains 1 uDENN domain.
Sequence caution	The sequence BAA20814.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of MAPK activity Inferred from mutant phenotype Ref.2. Source: UniProtKB apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cell surface receptor signaling pathway Traceable author statement Ref.2. Source: ProtInc regulation of apoptotic process Inferred from mutant phenotype Ref.4Ref.10Ref.2. Source: UniProtKB regulation of cell cycle Inferred from mutant phenotype Ref.10. Source: UniProtKB
Cellular_component	cytoplasm Traceable author statement Ref.1. Source: ProtInc integral to membrane Inferred from direct assay Ref.1. Source: UniProtKB plasma membrane Inferred from direct assay. Source: HPA
Molecular_function	Rab guanyl-nucleotide exchange factor activity Inferred from direct assay Ref.17. Source: UniProtKB death receptor binding Traceable author statement Ref.2. Source: ProtInc protein kinase activator activity Traceable author statement Ref.2. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PTPRN	Q16849	4	EBI-310528,EBI-728153

Alternative products

This entry describes 8 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.4 (identifier: Q8WXG6-1)

Also known as: IG20; IG20-FL;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.4 (identifier: Q8WXG6-2)

Also known as: IG20; IG20-PA; IG20-PASV;

The sequence of this isoform differs from the canonical sequence as follows:
     1121-1139: ELWNKHQEVKKQKALEKQR → G
     1291-1311: Missing.

Isoform 3 Ref.1 Ref.2 Ref.3 (identifier: Q8WXG6-3)

Also known as: DENN; IG20-SV1; MADD;

The sequence of this isoform differs from the canonical sequence as follows:
     885-904: Missing.
     1121-1139: ELWNKHQEVKKQKALEKQR → G
     1291-1311: Missing.

Isoform 4 Ref.1 Ref.3 Ref.4 (identifier: Q8WXG6-4)

Also known as: IG20-SV2;

The sequence of this isoform differs from the canonical sequence as follows:
     762-804: Missing.
     1121-1139: ELWNKHQEVKKQKALEKQR → G
     1291-1311: Missing.

Isoform 5 Ref.4 Ref.9 (identifier: Q8WXG6-5)

Also known as: DENN-SV; IG20-SV3;

The sequence of this isoform differs from the canonical sequence as follows:
     762-804: Missing.
     885-904: Missing.
     1121-1139: ELWNKHQEVKKQKALEKQR → G
     1291-1311: Missing.

Isoform 6 Ref.4 (identifier: Q8WXG6-6)

Also known as: IG20-SV4;

The sequence of this isoform differs from the canonical sequence as follows:
     762-804: Missing.
     885-904: Missing.
     1121-1139: ELWNKHQEVKKQKALEKQR → G
     1291-1311: Missing.
     1575-1582: VFIELNHI → FLKLKKW
     1583-1647: Missing.

Isoform 7 Ref.5 (identifier: Q8WXG6-7)

The sequence of this isoform differs from the canonical sequence as follows:
     1575-1582: VFIELNHI → FLKLKKW
     1583-1647: Missing.

Isoform 8 (identifier: Q8WXG6-8)

The sequence of this isoform differs from the canonical sequence as follows:
     762-804: Missing.
     885-904: Missing.
     1121-1139: ELWNKHQEVKKQKALEKQR → G
     1197-1200: Missing.
     1291-1311: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity UniProtKB O08873
	Chain	2 – 1647	1646	MAP kinase-activating death domain protein UniProtKB O08873		PRO_0000278138
Regions
	Domain	25 – 98	74	UDENN
	Domain	172 – 402	231	DENN
	Domain	485 – 558	74	dDENN
	Domain	1340 – 1415	76	Death
	Compositional bias	1157 – 1250	94	Ser-rich
Amino acid modifications
	Modified residue	818	1	Phosphoserine Ref.14
	Modified residue	820	1	Phosphoserine Ref.14
	Modified residue	921	1	Phosphoserine Ref.14
	Modified residue	1059	1	Phosphoserine By similarity UniProtKB Q80U28
	Modified residue	1061	1	Phosphothreonine By similarity UniProtKB Q80U28
Natural variations
	Alternative sequence	762 – 804	43	Missing in isoform 4, isoform 5, isoform 6 and isoform 8. Ref.1 Ref.3 Ref.4 Ref.9		VSP_052293
	Alternative sequence	885 – 904	20	Missing in isoform 3, isoform 5, isoform 6 and isoform 8. Ref.1 Ref.2 Ref.3 Ref.4 Ref.9		VSP_052294
	Alternative sequence	1121 – 1139	19	ELWNK…LEKQR → G in isoform 2, isoform 3, isoform 4, isoform 5, isoform 6 and isoform 8. Ref.1 Ref.2 Ref.3 Ref.4 Ref.9		VSP_052295
	Alternative sequence	1197 – 1200	4	Missing in isoform 8.		VSP_044848
	Alternative sequence	1291 – 1311	21	Missing in isoform 2, isoform 3, isoform 4, isoform 5, isoform 6 and isoform 8. Ref.1 Ref.2 Ref.3 Ref.4 Ref.9		VSP_052296
	Alternative sequence	1575 – 1582	8	VFIELNHI → FLKLKKW in isoform 6 and isoform 7. Ref.4 Ref.5		VSP_052297
	Alternative sequence	1583 – 1647	65	Missing in isoform 6 and isoform 7. Ref.4 Ref.5		VSP_052298
	Natural variant	696	1	P → T. Ref.9 Corresponds to variant rs17854007 [ dbSNP | Ensembl ].		VAR_030666
	Natural variant	751	1	V → M. Ref.2 Corresponds to variant rs1051006 [ dbSNP | Ensembl ].		VAR_030667
	Natural variant	765	1	R → Q. Corresponds to variant rs3736101 [ dbSNP | Ensembl ].		VAR_051148
	Natural variant	968	1	R → G. Ref.9 Corresponds to variant rs17854008 [ dbSNP | Ensembl ].		VAR_030668
	Natural variant	1040	1	L → F. Ref.9 Corresponds to variant rs17854009 [ dbSNP | Ensembl ].		VAR_030669
	Natural variant	1518	1	L → P. Corresponds to variant rs34534575 [ dbSNP | Ensembl ].		VAR_051149
Experimental info
	Sequence conflict	108	1	E → G in AAL40265. Ref.4
	Sequence conflict	108	1	E → G in AAL40266. Ref.4
	Sequence conflict	108	1	E → G in AAL40267. Ref.4
	Sequence conflict	108	1	E → G in AAL40268. Ref.4
	Sequence conflict	108	1	E → G in AAL35261. Ref.4
	Sequence conflict	145	1	L → F in AAL40265. Ref.4
	Sequence conflict	145	1	L → F in AAL40266. Ref.4
	Sequence conflict	145	1	L → F in AAL40267. Ref.4
	Sequence conflict	145	1	L → F in AAL40268. Ref.4
	Sequence conflict	145	1	L → F in AAL35261. Ref.4
	Sequence conflict	205	1	E → G in BAF85131. Ref.6
	Sequence conflict	312	1	V → L in AAL40265. Ref.4
	Sequence conflict	312	1	V → L in AAL40266. Ref.4
	Sequence conflict	312	1	V → L in AAL40267. Ref.4
	Sequence conflict	312	1	V → L in AAL40268. Ref.4
	Sequence conflict	312	1	V → L in AAL35261. Ref.4
	Sequence conflict	482	1	V → A in AAL40265. Ref.4
	Sequence conflict	482	1	V → A in AAL40266. Ref.4
	Sequence conflict	482	1	V → A in AAL40267. Ref.4
	Sequence conflict	482	1	V → A in AAL40268. Ref.4
	Sequence conflict	482	1	V → A in AAL35261. Ref.4
	Sequence conflict	500	1	A → V in BAF85131. Ref.6
	Sequence conflict	1201	1	Missing in AAD12154. Ref.1
	Sequence conflict	1201	1	Missing in AAH40484. Ref.8
	Sequence conflict	1262	1	S → C in AAL40265. Ref.4
	Sequence conflict	1262	1	S → C in AAL40266. Ref.4
	Sequence conflict	1262	1	S → C in AAL40267. Ref.4
	Sequence conflict	1262	1	S → C in AAL40268. Ref.4
	Sequence conflict	1262	1	S → C in AAL35261. Ref.4
	Sequence conflict	1592	1	F → S in BAF85131. Ref.6
	Sequence conflict	1639	1	R → G in BAF85131. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (IG20) (IG20-FL) [UniParc]. Last modified February 20, 2007. Version 2. Checksum: 2CA9A3519269757E	FASTA	1,647	183,303
	10 20 30 40 50 60 MVQKKKFCPR LLDYLVIVGA RHPSSDSVAQ TPELLRRYPL EDHTEFPLPP DVVFFCQPEG 70 80 90 100 110 120 CLSVRQRRMS LRDDTSFVFT LTDKDTGVTR YGICVNFYRS FQKRISKEKG EGGAGSRGKE 130 140 150 160 170 180 GTHATCASEE GGTESSESGS SLQPLSADST PDVNQSPRGK RRAKAGSRSR NSTLTSLCVL 190 200 210 220 230 240 SHYPFFSTFR ECLYTLKRLV DCCSERLLGK KLGIPRGVQR DTMWRIFTGS LLVEEKSSAL 250 260 270 280 290 300 LHDLREIEAW IYRLLRSPVP VSGQKRVDIE VLPQELQPAL TFALPDPSRF TLVDFPLHLP 310 320 330 340 350 360 LELLGVDACL QVLTCILLEH KVVLQSRDYN ALSMSVMAFV AMIYPLEYMF PVIPLLPTCM 370 380 390 400 410 420 ASAEQLLLAP TPYIIGVPAS FFLYKLDFKM PDDVWLVDLD SNRVIAPTNA EVLPILPEPE 430 440 450 460 470 480 SLELKKHLKQ ALASMSLNTQ PILNLEKFHE GQEIPLLLGR PSNDLQSTPS TEFNPLIYGN 490 500 510 520 530 540 DVDSVDVATR VAMVRFFNSA NVLQGFQMHT RTLRLFPRPV VAFQAGSFLA SRPRQTPFAE 550 560 570 580 590 600 KLARTQAVEY FGEWILNPTN YAFQRIHNNM FDPALIGDKP KWYAHQLQPI HYRVYDSNSQ 610 620 630 640 650 660 LAEALSVPPE RDSDSEPTDD SGSDSMDYDD SSSSYSSLGD FVSEMMKCDI NGDTPNVDPL 670 680 690 700 710 720 THAALGDASE VEIDELQNQK EAEEPGPDSE NSQENPPLRS SSSTTASSSP STVIHGANSE 730 740 750 760 770 780 PADSTEMDDK AAVGVSKPLP SVPPSIGKSN VDRRQAEIGE GSVRRRIYDN PYFEPQYGFP 790 800 810 820 830 840 PEEDEDEQGE SYTPRFSQHV SGNRAQKLLR PNSLRLASDS DAESDSRASS PNSTVSNTST 850 860 870 880 890 900 EGFGGIMSFA SSLYRNHSTS FSLSNLTLPT KGAREKATPF PSLKVFGLNT LMEIVTEAGP 910 920 930 940 950 960 GSGEGNRRAL VDQKSSVIKH SPTVKREPPS PQGRSSNSSE NQQFLKEVVH SVLDGQGVGW 970 980 990 1000 1010 1020 LNMKKVRRLL ESEQLRVFVL SKLNRMVQSE DDARQDIIPD VEISRKVYKG MLDLLKCTVL 1030 1040 1050 1060 1070 1080 SLEQSYAHAG LGGMASIFGL LEIAQTHYYS KEPDKRKRSP TESVNTPVGK DPGLAGRGDP 1090 1100 1110 1120 1130 1140 KAMAQLRVPQ LGPRAPSATG KGPKELDTRS LKEENFIASI ELWNKHQEVK KQKALEKQRP 1150 1160 1170 1180 1190 1200 EVIKPVFDLG ETEEKKSQIS ADSGVSLTSS SQRTDQDSVI GVSPAVMIRS SSQDSEVSTV 1210 1220 1230 1240 1250 1260 VSNSSGETLG ADSDLSSNAG DGPGGEGSVH LASSRGTLSD SEIETNSATS TIFGKAHSLK 1270 1280 1290 1300 1310 1320 PSIKEKLAGS PIRTSEDVSQ RVYLYEGLLG RDKGSMWDQL EDAAMETFSI SKERSTLWDQ 1330 1340 1350 1360 1370 1380 MQFWEDAFLD AVMLEREGMG MDQGPQEMID RYLSLGEHDR KRLEDDEDRL LATLLHNLIS 1390 1400 1410 1420 1430 1440 YMLLMKVNKN DIRKKVRRLM GKSHIGLVYS QQINEVLDQL ANLNGRDLSI WSSGSRHMKK 1450 1460 1470 1480 1490 1500 QTFVVHAGTD TNGDIFFMEV CDDCVVLRSN IGTVYERWWY EKLINMTYCP KTKVLCLWRR 1510 1520 1530 1540 1550 1560 NGSETQLNKF YTKKCRELYY CVKDSMERAA ARQQSIKPGP ELGGEFPVQD LKTGEGGLLQ 1570 1580 1590 1600 1610 1620 VTLEGINLKF MHNQVFIELN HIKKCNTVRG VFVLEEFVPE IKEVVSHKYK TPMAHEICYS 1630 1640 VLCLFSYVAA VHSSEEDLRT PPRPVSS
	Isoform 2 (IG20) (IG20-PA) (IG20-PASV) [UniParc]. Checksum: FE1C240F2B74E284	FASTA	1,608	178,558
	10 20 30 40 50 60 MVQKKKFCPR LLDYLVIVGA RHPSSDSVAQ TPELLRRYPL EDHTEFPLPP DVVFFCQPEG 70 80 90 100 110 120 CLSVRQRRMS LRDDTSFVFT LTDKDTGVTR YGICVNFYRS FQKRISKEKG EGGAGSRGKE 130 140 150 160 170 180 GTHATCASEE GGTESSESGS SLQPLSADST PDVNQSPRGK RRAKAGSRSR NSTLTSLCVL 190 200 210 220 230 240 SHYPFFSTFR ECLYTLKRLV DCCSERLLGK KLGIPRGVQR DTMWRIFTGS LLVEEKSSAL 250 260 270 280 290 300 LHDLREIEAW IYRLLRSPVP VSGQKRVDIE VLPQELQPAL TFALPDPSRF TLVDFPLHLP 310 320 330 340 350 360 LELLGVDACL QVLTCILLEH KVVLQSRDYN ALSMSVMAFV AMIYPLEYMF PVIPLLPTCM 370 380 390 400 410 420 ASAEQLLLAP TPYIIGVPAS FFLYKLDFKM PDDVWLVDLD SNRVIAPTNA EVLPILPEPE 430 440 450 460 470 480 SLELKKHLKQ ALASMSLNTQ PILNLEKFHE GQEIPLLLGR PSNDLQSTPS TEFNPLIYGN 490 500 510 520 530 540 DVDSVDVATR VAMVRFFNSA NVLQGFQMHT RTLRLFPRPV VAFQAGSFLA SRPRQTPFAE 550 560 570 580 590 600 KLARTQAVEY FGEWILNPTN YAFQRIHNNM FDPALIGDKP KWYAHQLQPI HYRVYDSNSQ 610 620 630 640 650 660 LAEALSVPPE RDSDSEPTDD SGSDSMDYDD SSSSYSSLGD FVSEMMKCDI NGDTPNVDPL 670 680 690 700 710 720 THAALGDASE VEIDELQNQK EAEEPGPDSE NSQENPPLRS SSSTTASSSP STVIHGANSE 730 740 750 760 770 780 PADSTEMDDK AAVGVSKPLP SVPPSIGKSN VDRRQAEIGE GSVRRRIYDN PYFEPQYGFP 790 800 810 820 830 840 PEEDEDEQGE SYTPRFSQHV SGNRAQKLLR PNSLRLASDS DAESDSRASS PNSTVSNTST 850 860 870 880 890 900 EGFGGIMSFA SSLYRNHSTS FSLSNLTLPT KGAREKATPF PSLKVFGLNT LMEIVTEAGP 910 920 930 940 950 960 GSGEGNRRAL VDQKSSVIKH SPTVKREPPS PQGRSSNSSE NQQFLKEVVH SVLDGQGVGW 970 980 990 1000 1010 1020 LNMKKVRRLL ESEQLRVFVL SKLNRMVQSE DDARQDIIPD VEISRKVYKG MLDLLKCTVL 1030 1040 1050 1060 1070 1080 SLEQSYAHAG LGGMASIFGL LEIAQTHYYS KEPDKRKRSP TESVNTPVGK DPGLAGRGDP 1090 1100 1110 1120 1130 1140 KAMAQLRVPQ LGPRAPSATG KGPKELDTRS LKEENFIASI GPEVIKPVFD LGETEEKKSQ 1150 1160 1170 1180 1190 1200 ISADSGVSLT SSSQRTDQDS VIGVSPAVMI RSSSQDSEVS TVVSNSSGET LGADSDLSSN 1210 1220 1230 1240 1250 1260 AGDGPGGEGS VHLASSRGTL SDSEIETNSA TSTIFGKAHS LKPSIKEKLA GSPIRTSEDV 1270 1280 1290 1300 1310 1320 SQRVYLYEGL LGKERSTLWD QMQFWEDAFL DAVMLEREGM GMDQGPQEMI DRYLSLGEHD 1330 1340 1350 1360 1370 1380 RKRLEDDEDR LLATLLHNLI SYMLLMKVNK NDIRKKVRRL MGKSHIGLVY SQQINEVLDQ 1390 1400 1410 1420 1430 1440 LANLNGRDLS IWSSGSRHMK KQTFVVHAGT DTNGDIFFME VCDDCVVLRS NIGTVYERWW 1450 1460 1470 1480 1490 1500 YEKLINMTYC PKTKVLCLWR RNGSETQLNK FYTKKCRELY YCVKDSMERA AARQQSIKPG 1510 1520 1530 1540 1550 1560 PELGGEFPVQ DLKTGEGGLL QVTLEGINLK FMHNQVFIEL NHIKKCNTVR GVFVLEEFVP 1570 1580 1590 1600 EIKEVVSHKY KTPMAHEICY SVLCLFSYVA AVHSSEEDLR TPPRPVSS
	Isoform 3 (DENN) (IG20-SV1) (MADD) [UniParc]. Checksum: 4D83DC2BDCD7AEAD	FASTA	1,588	176,555
	10 20 30 40 50 60 MVQKKKFCPR LLDYLVIVGA RHPSSDSVAQ TPELLRRYPL EDHTEFPLPP DVVFFCQPEG 70 80 90 100 110 120 CLSVRQRRMS LRDDTSFVFT LTDKDTGVTR YGICVNFYRS FQKRISKEKG EGGAGSRGKE 130 140 150 160 170 180 GTHATCASEE GGTESSESGS SLQPLSADST PDVNQSPRGK RRAKAGSRSR NSTLTSLCVL 190 200 210 220 230 240 SHYPFFSTFR ECLYTLKRLV DCCSERLLGK KLGIPRGVQR DTMWRIFTGS LLVEEKSSAL 250 260 270 280 290 300 LHDLREIEAW IYRLLRSPVP VSGQKRVDIE VLPQELQPAL TFALPDPSRF TLVDFPLHLP 310 320 330 340 350 360 LELLGVDACL QVLTCILLEH KVVLQSRDYN ALSMSVMAFV AMIYPLEYMF PVIPLLPTCM 370 380 390 400 410 420 ASAEQLLLAP TPYIIGVPAS FFLYKLDFKM PDDVWLVDLD SNRVIAPTNA EVLPILPEPE 430 440 450 460 470 480 SLELKKHLKQ ALASMSLNTQ PILNLEKFHE GQEIPLLLGR PSNDLQSTPS TEFNPLIYGN 490 500 510 520 530 540 DVDSVDVATR VAMVRFFNSA NVLQGFQMHT RTLRLFPRPV VAFQAGSFLA SRPRQTPFAE 550 560 570 580 590 600 KLARTQAVEY FGEWILNPTN YAFQRIHNNM FDPALIGDKP KWYAHQLQPI HYRVYDSNSQ 610 620 630 640 650 660 LAEALSVPPE RDSDSEPTDD SGSDSMDYDD SSSSYSSLGD FVSEMMKCDI NGDTPNVDPL 670 680 690 700 710 720 THAALGDASE VEIDELQNQK EAEEPGPDSE NSQENPPLRS SSSTTASSSP STVIHGANSE 730 740 750 760 770 780 PADSTEMDDK AAVGVSKPLP SVPPSIGKSN VDRRQAEIGE GSVRRRIYDN PYFEPQYGFP 790 800 810 820 830 840 PEEDEDEQGE SYTPRFSQHV SGNRAQKLLR PNSLRLASDS DAESDSRASS PNSTVSNTST 850 860 870 880 890 900 EGFGGIMSFA SSLYRNHSTS FSLSNLTLPT KGAREKATPF PSLKGNRRAL VDQKSSVIKH 910 920 930 940 950 960 SPTVKREPPS PQGRSSNSSE NQQFLKEVVH SVLDGQGVGW LNMKKVRRLL ESEQLRVFVL 970 980 990 1000 1010 1020 SKLNRMVQSE DDARQDIIPD VEISRKVYKG MLDLLKCTVL SLEQSYAHAG LGGMASIFGL 1030 1040 1050 1060 1070 1080 LEIAQTHYYS KEPDKRKRSP TESVNTPVGK DPGLAGRGDP KAMAQLRVPQ LGPRAPSATG 1090 1100 1110 1120 1130 1140 KGPKELDTRS LKEENFIASI GPEVIKPVFD LGETEEKKSQ ISADSGVSLT SSSQRTDQDS 1150 1160 1170 1180 1190 1200 VIGVSPAVMI RSSSQDSEVS TVVSNSSGET LGADSDLSSN AGDGPGGEGS VHLASSRGTL 1210 1220 1230 1240 1250 1260 SDSEIETNSA TSTIFGKAHS LKPSIKEKLA GSPIRTSEDV SQRVYLYEGL LGKERSTLWD 1270 1280 1290 1300 1310 1320 QMQFWEDAFL DAVMLEREGM GMDQGPQEMI DRYLSLGEHD RKRLEDDEDR LLATLLHNLI 1330 1340 1350 1360 1370 1380 SYMLLMKVNK NDIRKKVRRL MGKSHIGLVY SQQINEVLDQ LANLNGRDLS IWSSGSRHMK 1390 1400 1410 1420 1430 1440 KQTFVVHAGT DTNGDIFFME VCDDCVVLRS NIGTVYERWW YEKLINMTYC PKTKVLCLWR 1450 1460 1470 1480 1490 1500 RNGSETQLNK FYTKKCRELY YCVKDSMERA AARQQSIKPG PELGGEFPVQ DLKTGEGGLL 1510 1520 1530 1540 1550 1560 QVTLEGINLK FMHNQVFIEL NHIKKCNTVR GVFVLEEFVP EIKEVVSHKY KTPMAHEICY 1570 1580 SVLCLFSYVA AVHSSEEDLR TPPRPVSS
	Isoform 4 (IG20-SV2) [UniParc]. Checksum: BF80481B41A4E680	FASTA	1,565	173,395
	10 20 30 40 50 60 MVQKKKFCPR LLDYLVIVGA RHPSSDSVAQ TPELLRRYPL EDHTEFPLPP DVVFFCQPEG 70 80 90 100 110 120 CLSVRQRRMS LRDDTSFVFT LTDKDTGVTR YGICVNFYRS FQKRISKEKG EGGAGSRGKE 130 140 150 160 170 180 GTHATCASEE GGTESSESGS SLQPLSADST PDVNQSPRGK RRAKAGSRSR NSTLTSLCVL 190 200 210 220 230 240 SHYPFFSTFR ECLYTLKRLV DCCSERLLGK KLGIPRGVQR DTMWRIFTGS LLVEEKSSAL 250 260 270 280 290 300 LHDLREIEAW IYRLLRSPVP VSGQKRVDIE VLPQELQPAL TFALPDPSRF TLVDFPLHLP 310 320 330 340 350 360 LELLGVDACL QVLTCILLEH KVVLQSRDYN ALSMSVMAFV AMIYPLEYMF PVIPLLPTCM 370 380 390 400 410 420 ASAEQLLLAP TPYIIGVPAS FFLYKLDFKM PDDVWLVDLD SNRVIAPTNA EVLPILPEPE 430 440 450 460 470 480 SLELKKHLKQ ALASMSLNTQ PILNLEKFHE GQEIPLLLGR PSNDLQSTPS TEFNPLIYGN 490 500 510 520 530 540 DVDSVDVATR VAMVRFFNSA NVLQGFQMHT RTLRLFPRPV VAFQAGSFLA SRPRQTPFAE 550 560 570 580 590 600 KLARTQAVEY FGEWILNPTN YAFQRIHNNM FDPALIGDKP KWYAHQLQPI HYRVYDSNSQ 610 620 630 640 650 660 LAEALSVPPE RDSDSEPTDD SGSDSMDYDD SSSSYSSLGD FVSEMMKCDI NGDTPNVDPL 670 680 690 700 710 720 THAALGDASE VEIDELQNQK EAEEPGPDSE NSQENPPLRS SSSTTASSSP STVIHGANSE 730 740 750 760 770 780 PADSTEMDDK AAVGVSKPLP SVPPSIGKSN VDRRQAEIGE GAQKLLRPNS LRLASDSDAE 790 800 810 820 830 840 SDSRASSPNS TVSNTSTEGF GGIMSFASSL YRNHSTSFSL SNLTLPTKGA REKATPFPSL 850 860 870 880 890 900 KVFGLNTLME IVTEAGPGSG EGNRRALVDQ KSSVIKHSPT VKREPPSPQG RSSNSSENQQ 910 920 930 940 950 960 FLKEVVHSVL DGQGVGWLNM KKVRRLLESE QLRVFVLSKL NRMVQSEDDA RQDIIPDVEI 970 980 990 1000 1010 1020 SRKVYKGMLD LLKCTVLSLE QSYAHAGLGG MASIFGLLEI AQTHYYSKEP DKRKRSPTES 1030 1040 1050 1060 1070 1080 VNTPVGKDPG LAGRGDPKAM AQLRVPQLGP RAPSATGKGP KELDTRSLKE ENFIASIGPE 1090 1100 1110 1120 1130 1140 VIKPVFDLGE TEEKKSQISA DSGVSLTSSS QRTDQDSVIG VSPAVMIRSS SQDSEVSTVV 1150 1160 1170 1180 1190 1200 SNSSGETLGA DSDLSSNAGD GPGGEGSVHL ASSRGTLSDS EIETNSATST IFGKAHSLKP 1210 1220 1230 1240 1250 1260 SIKEKLAGSP IRTSEDVSQR VYLYEGLLGK ERSTLWDQMQ FWEDAFLDAV MLEREGMGMD 1270 1280 1290 1300 1310 1320 QGPQEMIDRY LSLGEHDRKR LEDDEDRLLA TLLHNLISYM LLMKVNKNDI RKKVRRLMGK 1330 1340 1350 1360 1370 1380 SHIGLVYSQQ INEVLDQLAN LNGRDLSIWS SGSRHMKKQT FVVHAGTDTN GDIFFMEVCD 1390 1400 1410 1420 1430 1440 DCVVLRSNIG TVYERWWYEK LINMTYCPKT KVLCLWRRNG SETQLNKFYT KKCRELYYCV 1450 1460 1470 1480 1490 1500 KDSMERAAAR QQSIKPGPEL GGEFPVQDLK TGEGGLLQVT LEGINLKFMH NQVFIELNHI 1510 1520 1530 1540 1550 1560 KKCNTVRGVF VLEEFVPEIK EVVSHKYKTP MAHEICYSVL CLFSYVAAVH SSEEDLRTPP RPVSS
	Isoform 5 (DENN-SV) (IG20-SV3) [UniParc]. Checksum: 30610743419A2E69	FASTA	1,545	171,392
	10 20 30 40 50 60 MVQKKKFCPR LLDYLVIVGA RHPSSDSVAQ TPELLRRYPL EDHTEFPLPP DVVFFCQPEG 70 80 90 100 110 120 CLSVRQRRMS LRDDTSFVFT LTDKDTGVTR YGICVNFYRS FQKRISKEKG EGGAGSRGKE 130 140 150 160 170 180 GTHATCASEE GGTESSESGS SLQPLSADST PDVNQSPRGK RRAKAGSRSR NSTLTSLCVL 190 200 210 220 230 240 SHYPFFSTFR ECLYTLKRLV DCCSERLLGK KLGIPRGVQR DTMWRIFTGS LLVEEKSSAL 250 260 270 280 290 300 LHDLREIEAW IYRLLRSPVP VSGQKRVDIE VLPQELQPAL TFALPDPSRF TLVDFPLHLP 310 320 330 340 350 360 LELLGVDACL QVLTCILLEH KVVLQSRDYN ALSMSVMAFV AMIYPLEYMF PVIPLLPTCM 370 380 390 400 410 420 ASAEQLLLAP TPYIIGVPAS FFLYKLDFKM PDDVWLVDLD SNRVIAPTNA EVLPILPEPE 430 440 450 460 470 480 SLELKKHLKQ ALASMSLNTQ PILNLEKFHE GQEIPLLLGR PSNDLQSTPS TEFNPLIYGN 490 500 510 520 530 540 DVDSVDVATR VAMVRFFNSA NVLQGFQMHT RTLRLFPRPV VAFQAGSFLA SRPRQTPFAE 550 560 570 580 590 600 KLARTQAVEY FGEWILNPTN YAFQRIHNNM FDPALIGDKP KWYAHQLQPI HYRVYDSNSQ 610 620 630 640 650 660 LAEALSVPPE RDSDSEPTDD SGSDSMDYDD SSSSYSSLGD FVSEMMKCDI NGDTPNVDPL 670 680 690 700 710 720 THAALGDASE VEIDELQNQK EAEEPGPDSE NSQENPPLRS SSSTTASSSP STVIHGANSE 730 740 750 760 770 780 PADSTEMDDK AAVGVSKPLP SVPPSIGKSN VDRRQAEIGE GAQKLLRPNS LRLASDSDAE 790 800 810 820 830 840 SDSRASSPNS TVSNTSTEGF GGIMSFASSL YRNHSTSFSL SNLTLPTKGA REKATPFPSL 850 860 870 880 890 900 KGNRRALVDQ KSSVIKHSPT VKREPPSPQG RSSNSSENQQ FLKEVVHSVL DGQGVGWLNM 910 920 930 940 950 960 KKVRRLLESE QLRVFVLSKL NRMVQSEDDA RQDIIPDVEI SRKVYKGMLD LLKCTVLSLE 970 980 990 1000 1010 1020 QSYAHAGLGG MASIFGLLEI AQTHYYSKEP DKRKRSPTES VNTPVGKDPG LAGRGDPKAM 1030 1040 1050 1060 1070 1080 AQLRVPQLGP RAPSATGKGP KELDTRSLKE ENFIASIGPE VIKPVFDLGE TEEKKSQISA 1090 1100 1110 1120 1130 1140 DSGVSLTSSS QRTDQDSVIG VSPAVMIRSS SQDSEVSTVV SNSSGETLGA DSDLSSNAGD 1150 1160 1170 1180 1190 1200 GPGGEGSVHL ASSRGTLSDS EIETNSATST IFGKAHSLKP SIKEKLAGSP IRTSEDVSQR 1210 1220 1230 1240 1250 1260 VYLYEGLLGK ERSTLWDQMQ FWEDAFLDAV MLEREGMGMD QGPQEMIDRY LSLGEHDRKR 1270 1280 1290 1300 1310 1320 LEDDEDRLLA TLLHNLISYM LLMKVNKNDI RKKVRRLMGK SHIGLVYSQQ INEVLDQLAN 1330 1340 1350 1360 1370 1380 LNGRDLSIWS SGSRHMKKQT FVVHAGTDTN GDIFFMEVCD DCVVLRSNIG TVYERWWYEK 1390 1400 1410 1420 1430 1440 LINMTYCPKT KVLCLWRRNG SETQLNKFYT KKCRELYYCV KDSMERAAAR QQSIKPGPEL 1450 1460 1470 1480 1490 1500 GGEFPVQDLK TGEGGLLQVT LEGINLKFMH NQVFIELNHI KKCNTVRGVF VLEEFVPEIK 1510 1520 1530 1540 EVVSHKYKTP MAHEICYSVL CLFSYVAAVH SSEEDLRTPP RPVSS
	Isoform 6 (IG20-SV4) [UniParc]. Checksum: 7D5CECD86F93B07D	FASTA	1,479	164,006
	10 20 30 40 50 60 MVQKKKFCPR LLDYLVIVGA RHPSSDSVAQ TPELLRRYPL EDHTEFPLPP DVVFFCQPEG 70 80 90 100 110 120 CLSVRQRRMS LRDDTSFVFT LTDKDTGVTR YGICVNFYRS FQKRISKEKG EGGAGSRGKE 130 140 150 160 170 180 GTHATCASEE GGTESSESGS SLQPLSADST PDVNQSPRGK RRAKAGSRSR NSTLTSLCVL 190 200 210 220 230 240 SHYPFFSTFR ECLYTLKRLV DCCSERLLGK KLGIPRGVQR DTMWRIFTGS LLVEEKSSAL 250 260 270 280 290 300 LHDLREIEAW IYRLLRSPVP VSGQKRVDIE VLPQELQPAL TFALPDPSRF TLVDFPLHLP 310 320 330 340 350 360 LELLGVDACL QVLTCILLEH KVVLQSRDYN ALSMSVMAFV AMIYPLEYMF PVIPLLPTCM 370 380 390 400 410 420 ASAEQLLLAP TPYIIGVPAS FFLYKLDFKM PDDVWLVDLD SNRVIAPTNA EVLPILPEPE 430 440 450 460 470 480 SLELKKHLKQ ALASMSLNTQ PILNLEKFHE GQEIPLLLGR PSNDLQSTPS TEFNPLIYGN 490 500 510 520 530 540 DVDSVDVATR VAMVRFFNSA NVLQGFQMHT RTLRLFPRPV VAFQAGSFLA SRPRQTPFAE 550 560 570 580 590 600 KLARTQAVEY FGEWILNPTN YAFQRIHNNM FDPALIGDKP KWYAHQLQPI HYRVYDSNSQ 610 620 630 640 650 660 LAEALSVPPE RDSDSEPTDD SGSDSMDYDD SSSSYSSLGD FVSEMMKCDI NGDTPNVDPL 670 680 690 700 710 720 THAALGDASE VEIDELQNQK EAEEPGPDSE NSQENPPLRS SSSTTASSSP STVIHGANSE 730 740 750 760 770 780 PADSTEMDDK AAVGVSKPLP SVPPSIGKSN VDRRQAEIGE GAQKLLRPNS LRLASDSDAE 790 800 810 820 830 840 SDSRASSPNS TVSNTSTEGF GGIMSFASSL YRNHSTSFSL SNLTLPTKGA REKATPFPSL 850 860 870 880 890 900 KGNRRALVDQ KSSVIKHSPT VKREPPSPQG RSSNSSENQQ FLKEVVHSVL DGQGVGWLNM 910 920 930 940 950 960 KKVRRLLESE QLRVFVLSKL NRMVQSEDDA RQDIIPDVEI SRKVYKGMLD LLKCTVLSLE 970 980 990 1000 1010 1020 QSYAHAGLGG MASIFGLLEI AQTHYYSKEP DKRKRSPTES VNTPVGKDPG LAGRGDPKAM 1030 1040 1050 1060 1070 1080 AQLRVPQLGP RAPSATGKGP KELDTRSLKE ENFIASIGPE VIKPVFDLGE TEEKKSQISA 1090 1100 1110 1120 1130 1140 DSGVSLTSSS QRTDQDSVIG VSPAVMIRSS SQDSEVSTVV SNSSGETLGA DSDLSSNAGD 1150 1160 1170 1180 1190 1200 GPGGEGSVHL ASSRGTLSDS EIETNSATST IFGKAHSLKP SIKEKLAGSP IRTSEDVSQR 1210 1220 1230 1240 1250 1260 VYLYEGLLGK ERSTLWDQMQ FWEDAFLDAV MLEREGMGMD QGPQEMIDRY LSLGEHDRKR 1270 1280 1290 1300 1310 1320 LEDDEDRLLA TLLHNLISYM LLMKVNKNDI RKKVRRLMGK SHIGLVYSQQ INEVLDQLAN 1330 1340 1350 1360 1370 1380 LNGRDLSIWS SGSRHMKKQT FVVHAGTDTN GDIFFMEVCD DCVVLRSNIG TVYERWWYEK 1390 1400 1410 1420 1430 1440 LINMTYCPKT KVLCLWRRNG SETQLNKFYT KKCRELYYCV KDSMERAAAR QQSIKPGPEL 1450 1460 1470 GGEFPVQDLK TGEGGLLQVT LEGINLKFMH NQFLKLKKW
	Isoform 7 [UniParc]. Checksum: 8E2F7CD03C349AC5	FASTA	1,581	175,917
	10 20 30 40 50 60 MVQKKKFCPR LLDYLVIVGA RHPSSDSVAQ TPELLRRYPL EDHTEFPLPP DVVFFCQPEG 70 80 90 100 110 120 CLSVRQRRMS LRDDTSFVFT LTDKDTGVTR YGICVNFYRS FQKRISKEKG EGGAGSRGKE 130 140 150 160 170 180 GTHATCASEE GGTESSESGS SLQPLSADST PDVNQSPRGK RRAKAGSRSR NSTLTSLCVL 190 200 210 220 230 240 SHYPFFSTFR ECLYTLKRLV DCCSERLLGK KLGIPRGVQR DTMWRIFTGS LLVEEKSSAL 250 260 270 280 290 300 LHDLREIEAW IYRLLRSPVP VSGQKRVDIE VLPQELQPAL TFALPDPSRF TLVDFPLHLP 310 320 330 340 350 360 LELLGVDACL QVLTCILLEH KVVLQSRDYN ALSMSVMAFV AMIYPLEYMF PVIPLLPTCM 370 380 390 400 410 420 ASAEQLLLAP TPYIIGVPAS FFLYKLDFKM PDDVWLVDLD SNRVIAPTNA EVLPILPEPE 430 440 450 460 470 480 SLELKKHLKQ ALASMSLNTQ PILNLEKFHE GQEIPLLLGR PSNDLQSTPS TEFNPLIYGN 490 500 510 520 530 540 DVDSVDVATR VAMVRFFNSA NVLQGFQMHT RTLRLFPRPV VAFQAGSFLA SRPRQTPFAE 550 560 570 580 590 600 KLARTQAVEY FGEWILNPTN YAFQRIHNNM FDPALIGDKP KWYAHQLQPI HYRVYDSNSQ 610 620 630 640 650 660 LAEALSVPPE RDSDSEPTDD SGSDSMDYDD SSSSYSSLGD FVSEMMKCDI NGDTPNVDPL 670 680 690 700 710 720 THAALGDASE VEIDELQNQK EAEEPGPDSE NSQENPPLRS SSSTTASSSP STVIHGANSE 730 740 750 760 770 780 PADSTEMDDK AAVGVSKPLP SVPPSIGKSN VDRRQAEIGE GSVRRRIYDN PYFEPQYGFP 790 800 810 820 830 840 PEEDEDEQGE SYTPRFSQHV SGNRAQKLLR PNSLRLASDS DAESDSRASS PNSTVSNTST 850 860 870 880 890 900 EGFGGIMSFA SSLYRNHSTS FSLSNLTLPT KGAREKATPF PSLKVFGLNT LMEIVTEAGP 910 920 930 940 950 960 GSGEGNRRAL VDQKSSVIKH SPTVKREPPS PQGRSSNSSE NQQFLKEVVH SVLDGQGVGW 970 980 990 1000 1010 1020 LNMKKVRRLL ESEQLRVFVL SKLNRMVQSE DDARQDIIPD VEISRKVYKG MLDLLKCTVL 1030 1040 1050 1060 1070 1080 SLEQSYAHAG LGGMASIFGL LEIAQTHYYS KEPDKRKRSP TESVNTPVGK DPGLAGRGDP 1090 1100 1110 1120 1130 1140 KAMAQLRVPQ LGPRAPSATG KGPKELDTRS LKEENFIASI ELWNKHQEVK KQKALEKQRP 1150 1160 1170 1180 1190 1200 EVIKPVFDLG ETEEKKSQIS ADSGVSLTSS SQRTDQDSVI GVSPAVMIRS SSQDSEVSTV 1210 1220 1230 1240 1250 1260 VSNSSGETLG ADSDLSSNAG DGPGGEGSVH LASSRGTLSD SEIETNSATS TIFGKAHSLK 1270 1280 1290 1300 1310 1320 PSIKEKLAGS PIRTSEDVSQ RVYLYEGLLG RDKGSMWDQL EDAAMETFSI SKERSTLWDQ 1330 1340 1350 1360 1370 1380 MQFWEDAFLD AVMLEREGMG MDQGPQEMID RYLSLGEHDR KRLEDDEDRL LATLLHNLIS 1390 1400 1410 1420 1430 1440 YMLLMKVNKN DIRKKVRRLM GKSHIGLVYS QQINEVLDQL ANLNGRDLSI WSSGSRHMKK 1450 1460 1470 1480 1490 1500 QTFVVHAGTD TNGDIFFMEV CDDCVVLRSN IGTVYERWWY EKLINMTYCP KTKVLCLWRR 1510 1520 1530 1540 1550 1560 NGSETQLNKF YTKKCRELYY CVKDSMERAA ARQQSIKPGP ELGGEFPVQD LKTGEGGLLQ 1570 1580 VTLEGINLKF MHNQFLKLKK W
	Isoform 8 [UniParc]. Checksum: F717C904CE01ADE1	FASTA	1,541	171,006
	10 20 30 40 50 60 MVQKKKFCPR LLDYLVIVGA RHPSSDSVAQ TPELLRRYPL EDHTEFPLPP DVVFFCQPEG 70 80 90 100 110 120 CLSVRQRRMS LRDDTSFVFT LTDKDTGVTR YGICVNFYRS FQKRISKEKG EGGAGSRGKE 130 140 150 160 170 180 GTHATCASEE GGTESSESGS SLQPLSADST PDVNQSPRGK RRAKAGSRSR NSTLTSLCVL 190 200 210 220 230 240 SHYPFFSTFR ECLYTLKRLV DCCSERLLGK KLGIPRGVQR DTMWRIFTGS LLVEEKSSAL 250 260 270 280 290 300 LHDLREIEAW IYRLLRSPVP VSGQKRVDIE VLPQELQPAL TFALPDPSRF TLVDFPLHLP 310 320 330 340 350 360 LELLGVDACL QVLTCILLEH KVVLQSRDYN ALSMSVMAFV AMIYPLEYMF PVIPLLPTCM 370 380 390 400 410 420 ASAEQLLLAP TPYIIGVPAS FFLYKLDFKM PDDVWLVDLD SNRVIAPTNA EVLPILPEPE 430 440 450 460 470 480 SLELKKHLKQ ALASMSLNTQ PILNLEKFHE GQEIPLLLGR PSNDLQSTPS TEFNPLIYGN 490 500 510 520 530 540 DVDSVDVATR VAMVRFFNSA NVLQGFQMHT RTLRLFPRPV VAFQAGSFLA SRPRQTPFAE 550 560 570 580 590 600 KLARTQAVEY FGEWILNPTN YAFQRIHNNM FDPALIGDKP KWYAHQLQPI HYRVYDSNSQ 610 620 630 640 650 660 LAEALSVPPE RDSDSEPTDD SGSDSMDYDD SSSSYSSLGD FVSEMMKCDI NGDTPNVDPL 670 680 690 700 710 720 THAALGDASE VEIDELQNQK EAEEPGPDSE NSQENPPLRS SSSTTASSSP STVIHGANSE 730 740 750 760 770 780 PADSTEMDDK AAVGVSKPLP SVPPSIGKSN VDRRQAEIGE GAQKLLRPNS LRLASDSDAE 790 800 810 820 830 840 SDSRASSPNS TVSNTSTEGF GGIMSFASSL YRNHSTSFSL SNLTLPTKGA REKATPFPSL 850 860 870 880 890 900 KGNRRALVDQ KSSVIKHSPT VKREPPSPQG RSSNSSENQQ FLKEVVHSVL DGQGVGWLNM 910 920 930 940 950 960 KKVRRLLESE QLRVFVLSKL NRMVQSEDDA RQDIIPDVEI SRKVYKGMLD LLKCTVLSLE 970 980 990 1000 1010 1020 QSYAHAGLGG MASIFGLLEI AQTHYYSKEP DKRKRSPTES VNTPVGKDPG LAGRGDPKAM 1030 1040 1050 1060 1070 1080 AQLRVPQLGP RAPSATGKGP KELDTRSLKE ENFIASIGPE VIKPVFDLGE TEEKKSQISA 1090 1100 1110 1120 1130 1140 DSGVSLTSSS QRTDQDSVIG VSPAVMIRSS SQDSEVSNSS GETLGADSDL SSNAGDGPGG 1150 1160 1170 1180 1190 1200 EGSVHLASSR GTLSDSEIET NSATSTIFGK AHSLKPSIKE KLAGSPIRTS EDVSQRVYLY 1210 1220 1230 1240 1250 1260 EGLLGKERST LWDQMQFWED AFLDAVMLER EGMGMDQGPQ EMIDRYLSLG EHDRKRLEDD 1270 1280 1290 1300 1310 1320 EDRLLATLLH NLISYMLLMK VNKNDIRKKV RRLMGKSHIG LVYSQQINEV LDQLANLNGR 1330 1340 1350 1360 1370 1380 DLSIWSSGSR HMKKQTFVVH AGTDTNGDIF FMEVCDDCVV LRSNIGTVYE RWWYEKLINM 1390 1400 1410 1420 1430 1440 TYCPKTKVLC LWRRNGSETQ LNKFYTKKCR ELYYCVKDSM ERAAARQQSI KPGPELGGEF 1450 1460 1470 1480 1490 1500 PVQDLKTGEG GLLQVTLEGI NLKFMHNQVF IELNHIKKCN TVRGVFVLEE FVPEIKEVVS 1510 1520 1530 1540 HKYKTPMAHE ICYSVLCLFS YVAAVHSSEE DLRTPPRPVS S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DENN, a novel human gene differentially expressed in normal and neoplastic cells." Chow V.T.K., Lee S.S. DNA Seq. 6:263-273(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Fetal liver.
[2]	"MADD, a novel death domain protein that interacts with the type 1 tumor necrosis factor receptor and activates mitogen-activated protein kinase." Schievella A.R., Chen J.H., Graham J.R., Lin L.-L. J. Biol. Chem. 272:12069-12075(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, INTERACTION WITH TNFRSF1A, VARIANT MET-751.
[3]	"The human DENN gene: genomic organization, alternative splicing, and localization to chromosome 11p11.21-p11.22." Chow V.T.K., Lim K.M., Lim D. Genome 41:543-552(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), TISSUE SPECIFICITY. Tissue: Liver.
[4]	"Contrasting effects of IG20 and its splice isoforms, MADD and DENN-SV, on tumor necrosis factor alpha-induced apoptosis and activation of caspase-8 and -3." Al-Zoubi A.M., Efimova E.V., Kaithamana S., Martinez O., El-Azami El-Idrissi M., Dogan R.E., Prabhakar B.S. J. Biol. Chem. 276:47202-47211(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 4; 5 AND 6), FUNCTION, INTERACTION WITH TNFRSF1A. Tissue: Insulinoma.
[5]	"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 4:141-150(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 7). Tissue: Brain.
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 8). Tissue: Testis.
[7]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5), VARIANTS THR-696; GLY-968 AND PHE-1040. Tissue: Testis.
[10]	"Antisense abrogation of DENN expression induces apoptosis of leukemia cells in vitro, causes tumor regression in vivo and alters the transcription of genes involved in apoptosis and the cell cycle." Lim K.M., Yeo W.S., Chow V.T.K. Int. J. Cancer 109:24-37(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"IG20, in contrast to DENN-SV, (MADD splice variants) suppresses tumor cell survival, and enhances their susceptibility to apoptosis and cancer drugs." Efimova E.V., Al-Zoubi A.M., Martinez O., Kaithamana S., Lu S., Arima T., Prabhakar B.S. Oncogene 23:1076-1087(2004) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING, FUNCTION, TISSUE SPECIFICITY.
[12]	"Down-regulation of DENN/MADD, a TNF receptor binding protein, correlates with neuronal cell death in Alzheimer's disease brain and hippocampal neurons." Del Villar K., Miller C.A. Proc. Natl. Acad. Sci. U.S.A. 101:4210-4215(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[13]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[14]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-818; SER-820 AND SER-921, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[15]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[17]	"Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors." Yoshimura S., Gerondopoulos A., Linford A., Rigden D.J., Barr F.A. J. Cell Biol. 191:367-381(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS GUANYL-NUCLEOTIDE EXCHANGE FACTOR.
[18]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[19]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U44953 mRNA. Translation: AAD12154.1. U77352 mRNA. Translation: AAB57735.1. AF440100 mRNA. Translation: AAL40265.1. AF440101 mRNA. Translation: AAL40266.1. AF440102 mRNA. Translation: AAL40267.1. AF440103 mRNA. Translation: AAL40268.1. AF440434 mRNA. Translation: AAL35261.1. AB002356 mRNA. Translation: BAA20814.2. Different initiation. AK292442 mRNA. Translation: BAF85131.1. AC018410 Genomic DNA. No translation available. AC090582 Genomic DNA. No translation available. CH471064 Genomic DNA. Translation: EAW67931.1. CH471064 Genomic DNA. Translation: EAW67932.1. BC040484 mRNA. Translation: AAH40484.1.
IPI	IPI00103536. IPI00107843. IPI00107844. IPI00237706. IPI00292094. IPI00292095. IPI00788877. IPI01012296.
RefSeq	NP_001129415.1. NM_001135943.1. NP_001129416.1. NM_001135944.1. NP_003673.3. NM_003682.3. NP_569826.2. NM_130470.2. NP_569827.2. NM_130471.2. NP_569828.2. NM_130472.2. NP_569829.2. NM_130473.2. NP_569830.2. NM_130474.2. NP_569831.1. NM_130475.2. NP_569832.2. NM_130476.2.
UniGene	Hs.82548.
3D structure databases
ProteinModelPortal	Q8WXG6.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8WXG6. 5 interactions.
PTM databases
PhosphoSite	Q8WXG6.
Polymorphism databases
DMDM	126215742.
Proteomic databases
PaxDb	Q8WXG6.
PRIDE	Q8WXG6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000311027; ENSP00000310933; ENSG00000110514. ENST00000342922; ENSP00000343902; ENSG00000110514. ENST00000349238; ENSP00000304505; ENSG00000110514. ENST00000395336; ENSP00000378745; ENSG00000110514. ENST00000395344; ENSP00000378753; ENSG00000110514. ENST00000402192; ENSP00000384287; ENSG00000110514. ENST00000402799; ENSP00000385585; ENSG00000110514. ENST00000406482; ENSP00000384435; ENSG00000110514. ENST00000407859; ENSP00000384204; ENSG00000110514.
GeneID	8567.
KEGG	hsa:8567.
UCSC	uc001neq.2. human. uc001ner.1. human. uc001nes.1. human. uc001net.1. human. uc001neu.1. human. uc001nev.1. human. uc001nex.2. human. uc009yln.1. human.
Organism-specific databases
CTD	8567.
GeneCards	GC11P047290.
HGNC	HGNC:6766. MADD.
HPA	HPA038568.
MIM	603584. gene+phenotype.
neXtProt	NX_Q8WXG6.
PharmGKB	PA30523.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG326239.
HOVERGEN	HBG079455.
InParanoid	Q8WXG6.
OMA	QVFIELN.
PhylomeDB	Q8WXG6.
Enzyme and pathway databases
Pathway_Interaction_DB	tnfpathway. TNF receptor signaling pathway.
Gene expression databases
ArrayExpress	Q8WXG6.
Bgee	Q8WXG6.
Genevestigator	Q8WXG6.
Family and domain databases
InterPro	IPR005112. dDENN_dom. IPR001194. DENN_dom. IPR005113. uDENN_dom. [Graphical view]
Pfam	PF03455. dDENN. 1 hit. PF02141. DENN. 1 hit. PF03456. uDENN. 1 hit. [Graphical view]
SMART	SM00801. dDENN. 1 hit. SM00799. DENN. 1 hit. SM00800. uDENN. 1 hit. [Graphical view]
PROSITE	PS50947. DDENN. 1 hit. PS50017. DEATH_DOMAIN. False negative. PS50211. DENN. 1 hit. PS50946. UDENN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	MADD. human.
GenomeRNAi	8567.
NextBio	32121.
SOURCE	Search...

Entry information

Entry name

MADD_HUMAN

Accession

Primary (citable) accession number: Q8WXG6
Secondary accession number(s): A8K8S7 , B5MEE5, D3DQR4, O15065, O15293, Q15732, Q15741, Q8IWD7, Q8WXG3, Q8WXG4, Q8WXG5, Q8WZ63

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	February 20, 2007
Last modified:	May 29, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.