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Q8WXF8 (DEDD2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-binding death effector domain-containing protein 2
Alternative name(s):
DED-containing protein FLAME-3
FADD-like anti-apoptotic molecule 3
Gene names
Name:DEDD2
Synonyms:FLAME3
ORF Names:PSEC0004
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a critical role in death receptor-induced apoptosis and may target CASP8 and CASP10 to the nucleus. May regulate degradation of intermediate filaments during apoptosis. May play a role in the general transcription machinery in the nucleus and might be an important regulator of the activity of GTF3C3.

Subunit structure

Interacts with CASP8, CASP10 and GTF3C3. Homodimerizes and heterodimerizes with DEDD. Ref.1 Ref.2 Ref.6

Subcellular location

Nucleusnucleolus. Note: Nuclear, accumulated in subnuclear structures resembling nucleoli. Ref.2

Tissue specificity

Expressed in most tissues. High levels were found in liver, kidney, heart, ovary, spleen, testes, skeletal muscle and peripheral blood leukocytes. Expression was absent or low in colon and small intestine. Expression is relatively high in the tumor cell lines chronic myologenous leukemia K-562 and the colorectal adenocarcinoma SW480. Expression is moderate in the cervical carcinoma HeLa, the Burkitt's lymphoma Raji, the lung carcinoma A-549, and the melanoma G-361. In contrast, two leukemia cell lines, HL-60 (promyelocytic leukemia) and MOLT-4 (lymphoblastic leukemia), show relatively low levels. Ref.1 Ref.2

Domain

Interactions with CASP8 and CASP10 are mediated by the DED domain.

Sequence similarities

Contains 1 DED (death effector) domain.

Sequence caution

The sequence AAH13372.2 differs from that shown. Reason: Frameshift at position 186.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8WXF8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8WXF8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     145-149: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 326326DNA-binding death effector domain-containing protein 2
PRO_0000191277

Regions

Domain25 – 10480DED
Motif104 – 1096Nuclear localization signal Potential
Motif155 – 17319Bipartite nuclear localization signal Potential

Natural variations

Alternative sequence145 – 1495Missing in isoform 2.
VSP_010312

Experimental info

Sequence conflict271H → N in AAM10835. Ref.2
Sequence conflict561A → G in AAM10835. Ref.2
Sequence conflict791D → G in BAC11551. Ref.4
Sequence conflict2071C → R in AAM10835. Ref.2
Sequence conflict2301Missing in AAH13372. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 3F7B0B307CC870CD

FASTA32636,179
        10         20         30         40         50         60 
MALSGSTPAP CWEEDECLDY YGMLSLHRMF EVVGGQLTEC ELELLAFLLD EAPGAAGGLA 

        70         80         90        100        110        120 
RARSGLELLL ELERRGQCDE SNLRLLGQLL RVLARHDLLP HLARKRRRPV SPERYSYGTS 

       130        140        150        160        170        180 
SSSKRTEGSC RRRRQSSSSA NSQQGQWETG SPPTKRQRRS RGRPSGGARR RRRGAPAAPQ 

       190        200        210        220        230        240 
QQSEPARPSS EGKVTCDIRL RVRAEYCEHG PALEQGVASR RPQALARQLD VFGQATAVLR 

       250        260        270        280        290        300 
SRDLGSVVCD IKFSELSYLD AFWGDYLSGA LLQALRGVFL TEALREAVGR EAVRLLVSVD 

       310        320 
EADYEAGRRR LLLMEEEGGR RPTEAS 

« Hide

Isoform 2 [UniParc].

Checksum: 2F0ABF19EF229FEE
Show »

FASTA32135,577

References

« Hide 'large scale' references
[1]"Identification and characterization of DEDD2, a death effector domain-containing protein."
Roth W., Stenner-Liewen F., Pawlowski K., Godzik A., Reed J.C.
J. Biol. Chem. 277:7501-7508(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH CASP8.
[2]"Death effector domain-containing proteins DEDD and FLAME-3 form nuclear complexes with the TFIIIC102 subunit of human transcription factor IIIC."
Zhan Y., Hegde R., Srinivasula S.M., Fernandes-Alnemri T., Alnemri E.S.
Cell Death Differ. 9:439-447(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH CASP8 AND GTF3C3.
[3]"DEDD regulates degradation of intermediate filaments during apoptosis."
Lee J.C., Schickling O., Stegh A.H., Oshima R.G., Dinsdale D., Cohen G.M., Peter M.E.
J. Cell Biol. 158:1051-1066(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. expand/collapse author list , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Teratocarcinoma.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain and Lung.
[6]"DEDD and DEDD2 associate with caspase-8/10 and signal cell death."
Alcivar A., Hu S., Tang J., Yang X.
Oncogene 22:291-297(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CASP8 AND CASP10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF443591 mRNA. Translation: AAL48220.1.
AF457575 mRNA. Translation: AAM10835.1.
AY125488 mRNA. Translation: AAM95240.1.
AK075328 mRNA. Translation: BAC11551.1.
BC013372 mRNA. Translation: AAH13372.2. Frameshift.
BC027930 mRNA. Translation: AAH27930.1.
CCDSCCDS12597.1. [Q8WXF8-1]
CCDS59391.1. [Q8WXF8-2]
RefSeqNP_001257543.1. NM_001270614.1. [Q8WXF8-1]
NP_001257544.1. NM_001270615.1. [Q8WXF8-2]
NP_579874.1. NM_133328.3. [Q8WXF8-1]
UniGeneHs.515432.
Hs.741580.

3D structure databases

ProteinModelPortalQ8WXF8.
SMRQ8WXF8. Positions 29-103.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127838. 12 interactions.
IntActQ8WXF8. 1 interaction.
STRING9606.ENSP00000336972.

PTM databases

PhosphoSiteQ8WXF8.

Polymorphism databases

DMDM47116024.

Proteomic databases

PaxDbQ8WXF8.
PRIDEQ8WXF8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000336034; ENSP00000336972; ENSG00000160570. [Q8WXF8-2]
ENST00000595337; ENSP00000470082; ENSG00000160570. [Q8WXF8-1]
ENST00000596251; ENSP00000471512; ENSG00000160570. [Q8WXF8-1]
GeneID162989.
KEGGhsa:162989.
UCSCuc002osu.2. human. [Q8WXF8-1]
uc002osw.2. human. [Q8WXF8-2]

Organism-specific databases

CTD162989.
GeneCardsGC19M042702.
HGNCHGNC:24450. DEDD2.
HPAHPA043419.
HPA043554.
neXtProtNX_Q8WXF8.
PharmGKBPA134912744.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG42334.
HOGENOMHOG000049120.
HOVERGENHBG007220.
InParanoidQ8WXF8.
OMASPERYNY.
PhylomeDBQ8WXF8.
TreeFamTF331807.

Gene expression databases

ArrayExpressQ8WXF8.
BgeeQ8WXF8.
CleanExHS_DEDD2.
GenevestigatorQ8WXF8.

Family and domain databases

Gene3D1.10.533.10. 1 hit.
InterProIPR011029. DEATH-like_dom.
IPR001875. DED.
[Graphical view]
PfamPF01335. DED. 1 hit.
[Graphical view]
SMARTSM00031. DED. 1 hit.
[Graphical view]
SUPFAMSSF47986. SSF47986. 1 hit.
PROSITEPS50168. DED. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi162989.
NextBio88251.
PROQ8WXF8.

Entry information

Entry nameDEDD2_HUMAN
AccessionPrimary (citable) accession number: Q8WXF8
Secondary accession number(s): Q8NBR2 expand/collapse secondary AC list , Q8NES1, Q8TAA8, Q96D35
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM