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Q8WXF8

- DEDD2_HUMAN

UniProt

Q8WXF8 - DEDD2_HUMAN

Protein

DNA-binding death effector domain-containing protein 2

Gene

DEDD2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    May play a critical role in death receptor-induced apoptosis and may target CASP8 and CASP10 to the nucleus. May regulate degradation of intermediate filaments during apoptosis. May play a role in the general transcription machinery in the nucleus and might be an important regulator of the activity of GTF3C3.

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: UniProtKB
    3. receptor signaling complex scaffold activity Source: UniProtKB

    GO - Biological processi

    1. apoptotic nuclear changes Source: UniProtKB
    2. cellular homeostasis Source: UniProtKB
    3. extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
    4. intracellular signal transduction Source: UniProtKB
    5. negative regulation of transcription, DNA-templated Source: UniProtKB
    6. positive regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
    7. RNA processing Source: UniProtKB
    8. rRNA catabolic process Source: UniProtKB
    9. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Apoptosis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-binding death effector domain-containing protein 2
    Alternative name(s):
    DED-containing protein FLAME-3
    FADD-like anti-apoptotic molecule 3
    Gene namesi
    Name:DEDD2
    Synonyms:FLAME3
    ORF Names:PSEC0004
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:24450. DEDD2.

    Subcellular locationi

    Nucleusnucleolus 1 Publication
    Note: Nuclear, accumulated in subnuclear structures resembling nucleoli.

    GO - Cellular componenti

    1. nucleolus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134912744.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 326326DNA-binding death effector domain-containing protein 2PRO_0000191277Add
    BLAST

    Proteomic databases

    PaxDbiQ8WXF8.
    PRIDEiQ8WXF8.

    PTM databases

    PhosphoSiteiQ8WXF8.

    Expressioni

    Tissue specificityi

    Expressed in most tissues. High levels were found in liver, kidney, heart, ovary, spleen, testes, skeletal muscle and peripheral blood leukocytes. Expression was absent or low in colon and small intestine. Expression is relatively high in the tumor cell lines chronic myologenous leukemia K-562 and the colorectal adenocarcinoma SW480. Expression is moderate in the cervical carcinoma HeLa, the Burkitt's lymphoma Raji, the lung carcinoma A-549, and the melanoma G-361. In contrast, two leukemia cell lines, HL-60 (promyelocytic leukemia) and MOLT-4 (lymphoblastic leukemia), show relatively low levels.2 Publications

    Gene expression databases

    ArrayExpressiQ8WXF8.
    BgeeiQ8WXF8.
    CleanExiHS_DEDD2.
    GenevestigatoriQ8WXF8.

    Organism-specific databases

    HPAiHPA043419.
    HPA043554.

    Interactioni

    Subunit structurei

    Interacts with CASP8, CASP10 and GTF3C3. Homodimerizes and heterodimerizes with DEDD.3 Publications

    Protein-protein interaction databases

    BioGridi127838. 12 interactions.
    IntActiQ8WXF8. 2 interactions.
    STRINGi9606.ENSP00000336972.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8WXF8.
    SMRiQ8WXF8. Positions 29-103.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 10480DEDPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi104 – 1096Nuclear localization signalSequence Analysis
    Motifi155 – 17319Bipartite nuclear localization signalSequence AnalysisAdd
    BLAST

    Domaini

    Interactions with CASP8 and CASP10 are mediated by the DED domain.

    Sequence similaritiesi

    Contains 1 DED (death effector) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG42334.
    HOGENOMiHOG000049120.
    HOVERGENiHBG007220.
    InParanoidiQ8WXF8.
    OMAiSPERYNY.
    PhylomeDBiQ8WXF8.
    TreeFamiTF331807.

    Family and domain databases

    Gene3Di1.10.533.10. 1 hit.
    InterProiIPR011029. DEATH-like_dom.
    IPR001875. DED.
    [Graphical view]
    PfamiPF01335. DED. 1 hit.
    [Graphical view]
    SMARTiSM00031. DED. 1 hit.
    [Graphical view]
    SUPFAMiSSF47986. SSF47986. 1 hit.
    PROSITEiPS50168. DED. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8WXF8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MALSGSTPAP CWEEDECLDY YGMLSLHRMF EVVGGQLTEC ELELLAFLLD    50
    EAPGAAGGLA RARSGLELLL ELERRGQCDE SNLRLLGQLL RVLARHDLLP 100
    HLARKRRRPV SPERYSYGTS SSSKRTEGSC RRRRQSSSSA NSQQGQWETG 150
    SPPTKRQRRS RGRPSGGARR RRRGAPAAPQ QQSEPARPSS EGKVTCDIRL 200
    RVRAEYCEHG PALEQGVASR RPQALARQLD VFGQATAVLR SRDLGSVVCD 250
    IKFSELSYLD AFWGDYLSGA LLQALRGVFL TEALREAVGR EAVRLLVSVD 300
    EADYEAGRRR LLLMEEEGGR RPTEAS 326
    Length:326
    Mass (Da):36,179
    Last modified:March 1, 2002 - v1
    Checksum:i3F7B0B307CC870CD
    GO
    Isoform 2 (identifier: Q8WXF8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         145-149: Missing.

    Show »
    Length:321
    Mass (Da):35,577
    Checksum:i2F0ABF19EF229FEE
    GO

    Sequence cautioni

    The sequence AAH13372.2 differs from that shown. Reason: Frameshift at position 186.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti27 – 271H → N in AAM10835. (PubMed:11965497)Curated
    Sequence conflicti56 – 561A → G in AAM10835. (PubMed:11965497)Curated
    Sequence conflicti79 – 791D → G in BAC11551. (PubMed:16303743)Curated
    Sequence conflicti207 – 2071C → R in AAM10835. (PubMed:11965497)Curated
    Sequence conflicti230 – 2301Missing in AAH13372. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei145 – 1495Missing in isoform 2. 2 PublicationsVSP_010312

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF443591 mRNA. Translation: AAL48220.1.
    AF457575 mRNA. Translation: AAM10835.1.
    AY125488 mRNA. Translation: AAM95240.1.
    AK075328 mRNA. Translation: BAC11551.1.
    BC013372 mRNA. Translation: AAH13372.2. Frameshift.
    BC027930 mRNA. Translation: AAH27930.1.
    CCDSiCCDS12597.1. [Q8WXF8-1]
    CCDS59391.1. [Q8WXF8-2]
    RefSeqiNP_001257543.1. NM_001270614.1. [Q8WXF8-1]
    NP_001257544.1. NM_001270615.1. [Q8WXF8-2]
    NP_579874.1. NM_133328.3. [Q8WXF8-1]
    UniGeneiHs.515432.
    Hs.741580.

    Genome annotation databases

    GeneIDi162989.
    KEGGihsa:162989.
    UCSCiuc002osu.2. human. [Q8WXF8-1]
    uc002osw.2. human. [Q8WXF8-2]

    Polymorphism databases

    DMDMi47116024.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF443591 mRNA. Translation: AAL48220.1 .
    AF457575 mRNA. Translation: AAM10835.1 .
    AY125488 mRNA. Translation: AAM95240.1 .
    AK075328 mRNA. Translation: BAC11551.1 .
    BC013372 mRNA. Translation: AAH13372.2 . Frameshift.
    BC027930 mRNA. Translation: AAH27930.1 .
    CCDSi CCDS12597.1. [Q8WXF8-1 ]
    CCDS59391.1. [Q8WXF8-2 ]
    RefSeqi NP_001257543.1. NM_001270614.1. [Q8WXF8-1 ]
    NP_001257544.1. NM_001270615.1. [Q8WXF8-2 ]
    NP_579874.1. NM_133328.3. [Q8WXF8-1 ]
    UniGenei Hs.515432.
    Hs.741580.

    3D structure databases

    ProteinModelPortali Q8WXF8.
    SMRi Q8WXF8. Positions 29-103.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 127838. 12 interactions.
    IntActi Q8WXF8. 2 interactions.
    STRINGi 9606.ENSP00000336972.

    PTM databases

    PhosphoSitei Q8WXF8.

    Polymorphism databases

    DMDMi 47116024.

    Proteomic databases

    PaxDbi Q8WXF8.
    PRIDEi Q8WXF8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 162989.
    KEGGi hsa:162989.
    UCSCi uc002osu.2. human. [Q8WXF8-1 ]
    uc002osw.2. human. [Q8WXF8-2 ]

    Organism-specific databases

    CTDi 162989.
    GeneCardsi GC19M042702.
    HGNCi HGNC:24450. DEDD2.
    HPAi HPA043419.
    HPA043554.
    neXtProti NX_Q8WXF8.
    PharmGKBi PA134912744.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG42334.
    HOGENOMi HOG000049120.
    HOVERGENi HBG007220.
    InParanoidi Q8WXF8.
    OMAi SPERYNY.
    PhylomeDBi Q8WXF8.
    TreeFami TF331807.

    Miscellaneous databases

    GenomeRNAii 162989.
    NextBioi 88251.
    PROi Q8WXF8.

    Gene expression databases

    ArrayExpressi Q8WXF8.
    Bgeei Q8WXF8.
    CleanExi HS_DEDD2.
    Genevestigatori Q8WXF8.

    Family and domain databases

    Gene3Di 1.10.533.10. 1 hit.
    InterProi IPR011029. DEATH-like_dom.
    IPR001875. DED.
    [Graphical view ]
    Pfami PF01335. DED. 1 hit.
    [Graphical view ]
    SMARTi SM00031. DED. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47986. SSF47986. 1 hit.
    PROSITEi PS50168. DED. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of DEDD2, a death effector domain-containing protein."
      Roth W., Stenner-Liewen F., Pawlowski K., Godzik A., Reed J.C.
      J. Biol. Chem. 277:7501-7508(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH CASP8.
    2. "Death effector domain-containing proteins DEDD and FLAME-3 form nuclear complexes with the TFIIIC102 subunit of human transcription factor IIIC."
      Zhan Y., Hegde R., Srinivasula S.M., Fernandes-Alnemri T., Alnemri E.S.
      Cell Death Differ. 9:439-447(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH CASP8 AND GTF3C3.
    3. "DEDD regulates degradation of intermediate filaments during apoptosis."
      Lee J.C., Schickling O., Stegh A.H., Oshima R.G., Dinsdale D., Cohen G.M., Peter M.E.
      J. Cell Biol. 158:1051-1066(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    4. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
      Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
      , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
      DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Teratocarcinoma.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Brain and Lung.
    6. "DEDD and DEDD2 associate with caspase-8/10 and signal cell death."
      Alcivar A., Hu S., Tang J., Yang X.
      Oncogene 22:291-297(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CASP8 AND CASP10.

    Entry informationi

    Entry nameiDEDD2_HUMAN
    AccessioniPrimary (citable) accession number: Q8WXF8
    Secondary accession number(s): Q8NBR2
    , Q8NES1, Q8TAA8, Q96D35
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3