Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA-binding death effector domain-containing protein 2

Gene

DEDD2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a critical role in death receptor-induced apoptosis and may target CASP8 and CASP10 to the nucleus. May regulate degradation of intermediate filaments during apoptosis. May play a role in the general transcription machinery in the nucleus and might be an important regulator of the activity of GTF3C3.

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • receptor signaling complex scaffold activity Source: UniProtKB

GO - Biological processi

  • apoptotic nuclear changes Source: UniProtKB
  • cellular homeostasis Source: UniProtKB
  • extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
  • intracellular signal transduction Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
  • RNA processing Source: UniProtKB
  • rRNA catabolic process Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding death effector domain-containing protein 2
Alternative name(s):
DED-containing protein FLAME-3
FADD-like anti-apoptotic molecule 3
Gene namesi
Name:DEDD2
Synonyms:FLAME3
ORF Names:PSEC0004
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:24450. DEDD2.

Subcellular locationi

  • Nucleusnucleolus 1 Publication

  • Note: Nuclear, accumulated in subnuclear structures resembling nucleoli.

GO - Cellular componenti

  • nucleolus Source: UniProtKB
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134912744.

Polymorphism and mutation databases

DMDMi47116024.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326DNA-binding death effector domain-containing protein 2PRO_0000191277Add
BLAST

Proteomic databases

PaxDbiQ8WXF8.
PRIDEiQ8WXF8.

PTM databases

PhosphoSiteiQ8WXF8.

Expressioni

Tissue specificityi

Expressed in most tissues. High levels were found in liver, kidney, heart, ovary, spleen, testes, skeletal muscle and peripheral blood leukocytes. Expression was absent or low in colon and small intestine. Expression is relatively high in the tumor cell lines chronic myologenous leukemia K-562 and the colorectal adenocarcinoma SW480. Expression is moderate in the cervical carcinoma HeLa, the Burkitt's lymphoma Raji, the lung carcinoma A-549, and the melanoma G-361. In contrast, two leukemia cell lines, HL-60 (promyelocytic leukemia) and MOLT-4 (lymphoblastic leukemia), show relatively low levels.2 Publications

Gene expression databases

BgeeiQ8WXF8.
CleanExiHS_DEDD2.
ExpressionAtlasiQ8WXF8. baseline and differential.
GenevisibleiQ8WXF8. HS.

Organism-specific databases

HPAiHPA043419.
HPA043554.

Interactioni

Subunit structurei

Interacts with CASP8, CASP10 and GTF3C3. Homodimerizes and heterodimerizes with DEDD.3 Publications

Protein-protein interaction databases

BioGridi127838. 13 interactions.
IntActiQ8WXF8. 2 interactions.
STRINGi9606.ENSP00000470082.

Structurei

3D structure databases

ProteinModelPortaliQ8WXF8.
SMRiQ8WXF8. Positions 29-103.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 10480DEDPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi104 – 1096Nuclear localization signalSequence Analysis
Motifi155 – 17319Bipartite nuclear localization signalSequence AnalysisAdd
BLAST

Domaini

Interactions with CASP8 and CASP10 are mediated by the DED domain.

Sequence similaritiesi

Contains 1 DED (death effector) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG42334.
GeneTreeiENSGT00390000008714.
HOGENOMiHOG000049120.
HOVERGENiHBG007220.
InParanoidiQ8WXF8.
OMAiAPCWEED.
PhylomeDBiQ8WXF8.
TreeFamiTF331807.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
InterProiIPR011029. DEATH-like_dom.
IPR001875. DED.
[Graphical view]
PfamiPF01335. DED. 1 hit.
[Graphical view]
SMARTiSM00031. DED. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
PROSITEiPS50168. DED. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WXF8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALSGSTPAP CWEEDECLDY YGMLSLHRMF EVVGGQLTEC ELELLAFLLD
60 70 80 90 100
EAPGAAGGLA RARSGLELLL ELERRGQCDE SNLRLLGQLL RVLARHDLLP
110 120 130 140 150
HLARKRRRPV SPERYSYGTS SSSKRTEGSC RRRRQSSSSA NSQQGQWETG
160 170 180 190 200
SPPTKRQRRS RGRPSGGARR RRRGAPAAPQ QQSEPARPSS EGKVTCDIRL
210 220 230 240 250
RVRAEYCEHG PALEQGVASR RPQALARQLD VFGQATAVLR SRDLGSVVCD
260 270 280 290 300
IKFSELSYLD AFWGDYLSGA LLQALRGVFL TEALREAVGR EAVRLLVSVD
310 320
EADYEAGRRR LLLMEEEGGR RPTEAS
Length:326
Mass (Da):36,179
Last modified:March 1, 2002 - v1
Checksum:i3F7B0B307CC870CD
GO
Isoform 2 (identifier: Q8WXF8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     145-149: Missing.

Show »
Length:321
Mass (Da):35,577
Checksum:i2F0ABF19EF229FEE
GO

Sequence cautioni

The sequence AAH13372.2 differs from that shown. Reason: Frameshift at position 186. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 271H → N in AAM10835 (PubMed:11965497).Curated
Sequence conflicti56 – 561A → G in AAM10835 (PubMed:11965497).Curated
Sequence conflicti79 – 791D → G in BAC11551 (PubMed:16303743).Curated
Sequence conflicti207 – 2071C → R in AAM10835 (PubMed:11965497).Curated
Sequence conflicti230 – 2301Missing in AAH13372 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei145 – 1495Missing in isoform 2. 2 PublicationsVSP_010312

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF443591 mRNA. Translation: AAL48220.1.
AF457575 mRNA. Translation: AAM10835.1.
AY125488 mRNA. Translation: AAM95240.1.
AK075328 mRNA. Translation: BAC11551.1.
BC013372 mRNA. Translation: AAH13372.2. Frameshift.
BC027930 mRNA. Translation: AAH27930.1.
CCDSiCCDS12597.1. [Q8WXF8-1]
CCDS59391.1. [Q8WXF8-2]
RefSeqiNP_001257543.1. NM_001270614.1. [Q8WXF8-1]
NP_001257544.1. NM_001270615.1. [Q8WXF8-2]
NP_579874.1. NM_133328.3. [Q8WXF8-1]
UniGeneiHs.515432.
Hs.741580.

Genome annotation databases

EnsembliENST00000336034; ENSP00000336972; ENSG00000160570. [Q8WXF8-2]
ENST00000595337; ENSP00000470082; ENSG00000160570.
ENST00000596251; ENSP00000471512; ENSG00000160570.
GeneIDi162989.
KEGGihsa:162989.
UCSCiuc002osu.2. human. [Q8WXF8-1]
uc002osw.2. human. [Q8WXF8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF443591 mRNA. Translation: AAL48220.1.
AF457575 mRNA. Translation: AAM10835.1.
AY125488 mRNA. Translation: AAM95240.1.
AK075328 mRNA. Translation: BAC11551.1.
BC013372 mRNA. Translation: AAH13372.2. Frameshift.
BC027930 mRNA. Translation: AAH27930.1.
CCDSiCCDS12597.1. [Q8WXF8-1]
CCDS59391.1. [Q8WXF8-2]
RefSeqiNP_001257543.1. NM_001270614.1. [Q8WXF8-1]
NP_001257544.1. NM_001270615.1. [Q8WXF8-2]
NP_579874.1. NM_133328.3. [Q8WXF8-1]
UniGeneiHs.515432.
Hs.741580.

3D structure databases

ProteinModelPortaliQ8WXF8.
SMRiQ8WXF8. Positions 29-103.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127838. 13 interactions.
IntActiQ8WXF8. 2 interactions.
STRINGi9606.ENSP00000470082.

PTM databases

PhosphoSiteiQ8WXF8.

Polymorphism and mutation databases

DMDMi47116024.

Proteomic databases

PaxDbiQ8WXF8.
PRIDEiQ8WXF8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336034; ENSP00000336972; ENSG00000160570. [Q8WXF8-2]
ENST00000595337; ENSP00000470082; ENSG00000160570.
ENST00000596251; ENSP00000471512; ENSG00000160570.
GeneIDi162989.
KEGGihsa:162989.
UCSCiuc002osu.2. human. [Q8WXF8-1]
uc002osw.2. human. [Q8WXF8-2]

Organism-specific databases

CTDi162989.
GeneCardsiGC19M042702.
HGNCiHGNC:24450. DEDD2.
HPAiHPA043419.
HPA043554.
neXtProtiNX_Q8WXF8.
PharmGKBiPA134912744.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG42334.
GeneTreeiENSGT00390000008714.
HOGENOMiHOG000049120.
HOVERGENiHBG007220.
InParanoidiQ8WXF8.
OMAiAPCWEED.
PhylomeDBiQ8WXF8.
TreeFamiTF331807.

Miscellaneous databases

ChiTaRSiDEDD2. human.
GenomeRNAii162989.
NextBioi88251.
PROiQ8WXF8.

Gene expression databases

BgeeiQ8WXF8.
CleanExiHS_DEDD2.
ExpressionAtlasiQ8WXF8. baseline and differential.
GenevisibleiQ8WXF8. HS.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
InterProiIPR011029. DEATH-like_dom.
IPR001875. DED.
[Graphical view]
PfamiPF01335. DED. 1 hit.
[Graphical view]
SMARTiSM00031. DED. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
PROSITEiPS50168. DED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of DEDD2, a death effector domain-containing protein."
    Roth W., Stenner-Liewen F., Pawlowski K., Godzik A., Reed J.C.
    J. Biol. Chem. 277:7501-7508(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH CASP8.
  2. "Death effector domain-containing proteins DEDD and FLAME-3 form nuclear complexes with the TFIIIC102 subunit of human transcription factor IIIC."
    Zhan Y., Hegde R., Srinivasula S.M., Fernandes-Alnemri T., Alnemri E.S.
    Cell Death Differ. 9:439-447(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH CASP8 AND GTF3C3.
  3. "DEDD regulates degradation of intermediate filaments during apoptosis."
    Lee J.C., Schickling O., Stegh A.H., Oshima R.G., Dinsdale D., Cohen G.M., Peter M.E.
    J. Cell Biol. 158:1051-1066(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  4. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
    Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
    , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
    DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Teratocarcinoma.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain and Lung.
  6. "DEDD and DEDD2 associate with caspase-8/10 and signal cell death."
    Alcivar A., Hu S., Tang J., Yang X.
    Oncogene 22:291-297(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CASP8 AND CASP10.

Entry informationi

Entry nameiDEDD2_HUMAN
AccessioniPrimary (citable) accession number: Q8WXF8
Secondary accession number(s): Q8NBR2
, Q8NES1, Q8TAA8, Q96D35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: March 1, 2002
Last modified: July 22, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.