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Q8WXF3 (REL3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Relaxin-3
Alternative name(s):
Insulin-like peptide INSL7
Short name=Insulin-like peptide 7
Prorelaxin H3

Cleaved into the following 2 chains:

  1. Relaxin-3 B chain
  2. Relaxin-3 A chain
Gene names
Name:RLN3
Synonyms:INSL7, RXN3, ZINS4
ORF Names:UNQ6188/PRO20213
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length142 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 (GPCR135) and relaxin-3 receptor-2 (GPCR142).

Subunit structure

Heterodimer of a B chain and an A chain linked by two disulfide bonds.

Subcellular location

Secreted.

Sequence similarities

Belongs to the insulin family.

Sequence caution

The sequence AAQ88548.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionHormone
   PTMCleavage on pair of basic residues
Disulfide bond
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Traceable author statement. Source: Reactome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Ref.2
Peptide26 – 5227Relaxin-3 B chain By similarity
PRO_0000016082
Propeptide55 – 11864Connecting peptide By similarity
PRO_0000016083
Peptide119 – 14224Relaxin-3 A chain By similarity
PRO_0000016084

Amino acid modifications

Disulfide bond35 ↔ 129Interchain (between B and A chains) Ref.7
Disulfide bond47 ↔ 142Interchain (between B and A chains) Ref.7
Disulfide bond128 ↔ 133 Ref.7

Secondary structure

......... 142
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8WXF3 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 23A3E095034B31E4

FASTA14215,451
        10         20         30         40         50         60 
MARYMLLLLL AVWVLTGELW PGAEARAAPY GVRLCGREFI RAVIFTCGGS RWRRSDILAH 

        70         80         90        100        110        120 
EAMGDTFPDA DADEDSLAGE LDEAMGSSEW LALTKSPQAF YRGRPSWQGT PGVLRGSRDV 

       130        140 
LAGLSSSCCK WGCSKSEISS LC 

« Hide

References

« Hide 'large scale' references
[1]"Homo sapiens insulin homolog polypeptide."
Holloway J.L., Lok S., Jaspers S.R.
Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Production of recombinant human relaxin 3 in AtT20 cells."
Kizawa H., Nishi K., Ishibashi Y., Harada M., Asano T., Ito Y., Suzuki N., Hinuma S., Fujisawa Y., Onda H., Nishimura O., Fujino M.
Regul. Pept. 113:79-84(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 26-34 AND 119-127.
[3]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"H3 relaxin is a specific ligand for LGR7 and activates the receptor by interacting with both the ectodomain and the exoloop 2."
Sudo S., Kumagai J., Nishi S., Layfield S., Ferraro T., Bathgate R.A.D., Hsueh A.J.W.
J. Biol. Chem. 278:7855-7862(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LGR7.
[5]"Identification of relaxin-3/INSL7 as an endogenous ligand for the orphan G-protein coupled receptor GPCR135."
Liu C., Eriste E., Sutton S., Chen J., Roland B., Kuei C., Farmer N., Joernvall H., Sillard R., Lovenberg T.W.
J. Biol. Chem. 278:50754-50764(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GPCR135.
[6]"Identification of relaxin-3/INSL7 as a ligand for GPCR142."
Liu C., Chen J., Sutton S., Roland B., Kuei C., Farmer N., Sillard R., Lovenberg T.W.
J. Biol. Chem. 278:50765-50770(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GPCR142.
[7]"Solution structure and novel insights into the determinants of the receptor specificity of human relaxin-3."
Rosengren K.J., Lin F., Bathgate R.A., Tregear G.W., Daly N.L., Wade J.D., Craik D.J.
J. Biol. Chem. 281:5845-5851(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 26-142, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF447451 mRNA. Translation: AAL40345.1.
AB076563 mRNA. Translation: BAC53758.1.
AY358181 mRNA. Translation: AAQ88548.1. Different initiation.
CCDSCCDS12302.1.
RefSeqNP_543140.1. NM_080864.2.
UniGeneHs.352155.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2FHWNMR-A119-142[»]
B26-52[»]
2K1VNMR-B26-52[»]
ProteinModelPortalQ8WXF3.
SMRQ8WXF3. Positions 26-52.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125590. 3 interactions.
STRING9606.ENSP00000307040.

PTM databases

PhosphoSiteQ8WXF3.

Polymorphism databases

DMDM37999891.

Proteomic databases

PaxDbQ8WXF3.
PRIDEQ8WXF3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000431365; ENSP00000397415; ENSG00000171136.
GeneID117579.
KEGGhsa:117579.
UCSCuc002mxw.1. human.

Organism-specific databases

CTD117579.
GeneCardsGC19P014137.
HGNCHGNC:17135. RLN3.
HPAHPA043731.
MIM606855. gene.
neXtProtNX_Q8WXF3.
PharmGKBPA34401.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG45013.
HOGENOMHOG000111888.
HOVERGENHBG045048.
InParanoidQ8WXF3.
KOK05255.
OMAEARASPY.
OrthoDBEOG7H1JNG.
PhylomeDBQ8WXF3.
TreeFamTF333404.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressQ8WXF3.
BgeeQ8WXF3.
CleanExHS_RLN3.
GenevestigatorQ8WXF3.

Family and domain databases

InterProIPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSPR00276. INSULINFAMLY.
SMARTSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMSSF56994. SSF56994. 2 hits.
PROSITEPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8WXF3.
GenomeRNAi117579.
NextBio80214.
PROQ8WXF3.
SOURCESearch...

Entry information

Entry nameREL3_HUMAN
AccessionPrimary (citable) accession number: Q8WXF3
Secondary accession number(s): Q6UXW5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM