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Q8WXF3

- REL3_HUMAN

UniProt

Q8WXF3 - REL3_HUMAN

Protein

Relaxin-3

Gene

RLN3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 (GPCR135) and relaxin-3 receptor-2 (GPCR142).

    GO - Molecular functioni

    1. protein binding Source: IntAct

    Keywords - Molecular functioni

    Hormone

    Enzyme and pathway databases

    ReactomeiREACT_19231. G alpha (i) signalling events.
    REACT_19327. G alpha (s) signalling events.
    REACT_21314. Relaxin receptors.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Relaxin-3
    Alternative name(s):
    Insulin-like peptide INSL7
    Short name:
    Insulin-like peptide 7
    Prorelaxin H3
    Cleaved into the following 2 chains:
    Gene namesi
    Name:RLN3
    Synonyms:INSL7, RXN3, ZINS4
    ORF Names:UNQ6188/PRO20213
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:17135. RLN3.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: Reactome

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA34401.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 25251 PublicationAdd
    BLAST
    Peptidei26 – 5227Relaxin-3 B chainBy similarityPRO_0000016082Add
    BLAST
    Propeptidei55 – 11864Connecting peptideBy similarityPRO_0000016083Add
    BLAST
    Peptidei119 – 14224Relaxin-3 A chainBy similarityPRO_0000016084Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi35 ↔ 129Interchain (between B and A chains)1 Publication
    Disulfide bondi47 ↔ 142Interchain (between B and A chains)1 Publication
    Disulfide bondi128 ↔ 1331 Publication

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    Proteomic databases

    PaxDbiQ8WXF3.
    PRIDEiQ8WXF3.

    PTM databases

    PhosphoSiteiQ8WXF3.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8WXF3.
    BgeeiQ8WXF3.
    CleanExiHS_RLN3.
    GenevestigatoriQ8WXF3.

    Organism-specific databases

    HPAiHPA043731.

    Interactioni

    Subunit structurei

    Heterodimer of a B chain and an A chain linked by two disulfide bonds.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RXFP4Q8TDU99EBI-9519546,EBI-9519524

    Protein-protein interaction databases

    BioGridi125590. 3 interactions.
    IntActiQ8WXF3. 1 interaction.
    STRINGi9606.ENSP00000307040.

    Structurei

    Secondary structure

    1
    142
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi29 – 324
    Helixi37 – 4711
    Helixi120 – 13011
    Helixi135 – 1395

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FHWNMR-A119-142[»]
    B26-52[»]
    2K1VNMR-B26-52[»]
    ProteinModelPortaliQ8WXF3.
    SMRiQ8WXF3. Positions 26-52.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8WXF3.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the insulin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG45013.
    HOGENOMiHOG000111888.
    HOVERGENiHBG045048.
    InParanoidiQ8WXF3.
    KOiK05255.
    OMAiEARASPY.
    OrthoDBiEOG7H1JNG.
    PhylomeDBiQ8WXF3.
    TreeFamiTF333404.

    Family and domain databases

    InterProiIPR016179. Insulin-like.
    IPR022353. Insulin_CS.
    IPR022352. Insulin_family.
    [Graphical view]
    PfamiPF00049. Insulin. 1 hit.
    [Graphical view]
    PRINTSiPR00276. INSULINFAMLY.
    SMARTiSM00078. IlGF. 1 hit.
    [Graphical view]
    SUPFAMiSSF56994. SSF56994. 2 hits.
    PROSITEiPS00262. INSULIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8WXF3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARYMLLLLL AVWVLTGELW PGAEARAAPY GVRLCGREFI RAVIFTCGGS    50
    RWRRSDILAH EAMGDTFPDA DADEDSLAGE LDEAMGSSEW LALTKSPQAF 100
    YRGRPSWQGT PGVLRGSRDV LAGLSSSCCK WGCSKSEISS LC 142
    Length:142
    Mass (Da):15,451
    Last modified:March 1, 2002 - v1
    Checksum:i23A3E095034B31E4
    GO

    Sequence cautioni

    The sequence AAQ88548.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF447451 mRNA. Translation: AAL40345.1.
    AB076563 mRNA. Translation: BAC53758.1.
    AY358181 mRNA. Translation: AAQ88548.1. Different initiation.
    CCDSiCCDS12302.1.
    RefSeqiNP_543140.1. NM_080864.2.
    UniGeneiHs.352155.

    Genome annotation databases

    EnsembliENST00000431365; ENSP00000397415; ENSG00000171136.
    GeneIDi117579.
    KEGGihsa:117579.
    UCSCiuc002mxw.1. human.

    Polymorphism databases

    DMDMi37999891.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF447451 mRNA. Translation: AAL40345.1 .
    AB076563 mRNA. Translation: BAC53758.1 .
    AY358181 mRNA. Translation: AAQ88548.1 . Different initiation.
    CCDSi CCDS12302.1.
    RefSeqi NP_543140.1. NM_080864.2.
    UniGenei Hs.352155.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FHW NMR - A 119-142 [» ]
    B 26-52 [» ]
    2K1V NMR - B 26-52 [» ]
    ProteinModelPortali Q8WXF3.
    SMRi Q8WXF3. Positions 26-52.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 125590. 3 interactions.
    IntActi Q8WXF3. 1 interaction.
    STRINGi 9606.ENSP00000307040.

    PTM databases

    PhosphoSitei Q8WXF3.

    Polymorphism databases

    DMDMi 37999891.

    Proteomic databases

    PaxDbi Q8WXF3.
    PRIDEi Q8WXF3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000431365 ; ENSP00000397415 ; ENSG00000171136 .
    GeneIDi 117579.
    KEGGi hsa:117579.
    UCSCi uc002mxw.1. human.

    Organism-specific databases

    CTDi 117579.
    GeneCardsi GC19P014137.
    HGNCi HGNC:17135. RLN3.
    HPAi HPA043731.
    MIMi 606855. gene.
    neXtProti NX_Q8WXF3.
    PharmGKBi PA34401.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG45013.
    HOGENOMi HOG000111888.
    HOVERGENi HBG045048.
    InParanoidi Q8WXF3.
    KOi K05255.
    OMAi EARASPY.
    OrthoDBi EOG7H1JNG.
    PhylomeDBi Q8WXF3.
    TreeFami TF333404.

    Enzyme and pathway databases

    Reactomei REACT_19231. G alpha (i) signalling events.
    REACT_19327. G alpha (s) signalling events.
    REACT_21314. Relaxin receptors.

    Miscellaneous databases

    EvolutionaryTracei Q8WXF3.
    GenomeRNAii 117579.
    NextBioi 80214.
    PROi Q8WXF3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8WXF3.
    Bgeei Q8WXF3.
    CleanExi HS_RLN3.
    Genevestigatori Q8WXF3.

    Family and domain databases

    InterProi IPR016179. Insulin-like.
    IPR022353. Insulin_CS.
    IPR022352. Insulin_family.
    [Graphical view ]
    Pfami PF00049. Insulin. 1 hit.
    [Graphical view ]
    PRINTSi PR00276. INSULINFAMLY.
    SMARTi SM00078. IlGF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56994. SSF56994. 2 hits.
    PROSITEi PS00262. INSULIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Homo sapiens insulin homolog polypeptide."
      Holloway J.L., Lok S., Jaspers S.R.
      Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 26-34 AND 119-127.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "H3 relaxin is a specific ligand for LGR7 and activates the receptor by interacting with both the ectodomain and the exoloop 2."
      Sudo S., Kumagai J., Nishi S., Layfield S., Ferraro T., Bathgate R.A.D., Hsueh A.J.W.
      J. Biol. Chem. 278:7855-7862(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LGR7.
    5. "Identification of relaxin-3/INSL7 as an endogenous ligand for the orphan G-protein coupled receptor GPCR135."
      Liu C., Eriste E., Sutton S., Chen J., Roland B., Kuei C., Farmer N., Joernvall H., Sillard R., Lovenberg T.W.
      J. Biol. Chem. 278:50754-50764(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GPCR135.
    6. Cited for: INTERACTION WITH GPCR142.
    7. "Solution structure and novel insights into the determinants of the receptor specificity of human relaxin-3."
      Rosengren K.J., Lin F., Bathgate R.A., Tregear G.W., Daly N.L., Wade J.D., Craik D.J.
      J. Biol. Chem. 281:5845-5851(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 26-142, DISULFIDE BONDS.

    Entry informationi

    Entry nameiREL3_HUMAN
    AccessioniPrimary (citable) accession number: Q8WXF3
    Secondary accession number(s): Q6UXW5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 10, 2003
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3