Q8WXF3 (REL3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Relaxin-3

Alternative name(s):
Insulin-like peptide INSL7
Short name=Insulin-like peptide 7
Prorelaxin H3

Cleaved into the following 2 chains:

1. Relaxin-3 B chain
2. Relaxin-3 A chain

Gene names

Name:	RLN3
Synonyms:	INSL7, RXN3, ZINS4
ORF Names:	UNQ6188/PRO20213

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	142 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 (GPCR135) and relaxin-3 receptor-2 (GPCR142).
Subunit structure	Heterodimer of a B chain and an A chain linked by two disulfide bonds.
Subcellular location	Secreted.
Sequence similarities	Belongs to the insulin family.
Sequence caution	The sequence AAQ88548.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 25

25

Ref.2

Peptide

26 – 52

27

Relaxin-3 B chain By similarity

PRO_0000016082

Propeptide

55 – 118

64

Connecting peptide By similarity

PRO_0000016083

Peptide

119 – 142

24

Relaxin-3 A chain By similarity

PRO_0000016084

Amino acid modifications

Disulfide bond

35 ↔ 129

Interchain (between B and A chains) Ref.7

Disulfide bond

47 ↔ 142

Interchain (between B and A chains) Ref.7

Disulfide bond

128 ↔ 133

Ref.7

Secondary structure

1

.

.

.

142

Helix Strand Turn

Details...

Helix

36 – 47

12

Sequences

	Sequence		Length	Mass (Da)
	Q8WXF3 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 23A3E095034B31E4	FASTA	142	15,451
	10 20 30 40 50 60 MARYMLLLLL AVWVLTGELW PGAEARAAPY GVRLCGREFI RAVIFTCGGS RWRRSDILAH 70 80 90 100 110 120 EAMGDTFPDA DADEDSLAGE LDEAMGSSEW LALTKSPQAF YRGRPSWQGT PGVLRGSRDV 130 140 LAGLSSSCCK WGCSKSEISS LC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homo sapiens insulin homolog polypeptide." Holloway J.L., Lok S., Jaspers S.R. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Production of recombinant human relaxin 3 in AtT20 cells." Kizawa H., Nishi K., Ishibashi Y., Harada M., Asano T., Ito Y., Suzuki N., Hinuma S., Fujisawa Y., Onda H., Nishimura O., Fujino M. Regul. Pept. 113:79-84(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 26-34 AND 119-127.
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"H3 relaxin is a specific ligand for LGR7 and activates the receptor by interacting with both the ectodomain and the exoloop 2." Sudo S., Kumagai J., Nishi S., Layfield S., Ferraro T., Bathgate R.A.D., Hsueh A.J.W. J. Biol. Chem. 278:7855-7862(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH LGR7.
[5]	"Identification of relaxin-3/INSL7 as an endogenous ligand for the orphan G-protein coupled receptor GPCR135." Liu C., Eriste E., Sutton S., Chen J., Roland B., Kuei C., Farmer N., Joernvall H., Sillard R., Lovenberg T.W. J. Biol. Chem. 278:50754-50764(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GPCR135.
[6]	"Identification of relaxin-3/INSL7 as a ligand for GPCR142." Liu C., Chen J., Sutton S., Roland B., Kuei C., Farmer N., Sillard R., Lovenberg T.W. J. Biol. Chem. 278:50765-50770(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GPCR142.
[7]	"Solution structure and novel insights into the determinants of the receptor specificity of human relaxin-3." Rosengren K.J., Lin F., Bathgate R.A., Tregear G.W., Daly N.L., Wade J.D., Craik D.J. J. Biol. Chem. 281:5845-5851(2006) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 26-142, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF447451 mRNA. Translation: AAL40345.1.
AB076563 mRNA. Translation: BAC53758.1.
AY358181 mRNA. Translation: AAQ88548.1. Different initiation.

IPI

IPI00103527.

RefSeq

NP_543140.1. NM_080864.2.

UniGene

Hs.352155.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FHW	NMR	-	A	119-142	[»]
			B	26-52	[»]
2K1V	NMR	-	B	26-52	[»]

ProteinModelPortal

Q8WXF3.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000307040.

PTM databases

PhosphoSite

Q8WXF3.

Polymorphism databases

DMDM

37999891.

Proteomic databases

PaxDb

Q8WXF3.

PRIDE

Q8WXF3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000431365; ENSP00000397415; ENSG00000171136.

GeneID

117579.

KEGG

hsa:117579.

UCSC

uc002mxw.1. human.

Organism-specific databases

CTD

117579.

GeneCards

GC19P014137.

HGNC

HGNC:17135. RLN3.

HPA

HPA043731.

MIM

606855. gene.

neXtProt

NX_Q8WXF3.

PharmGKB

PA34401.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG45013.

HOGENOM

HOG000111888.

HOVERGEN

HBG045048.

InParanoid

Q8WXF3.

KO

K05255.

OMA

RPSWQGT.

OrthoDB

EOG4VHK7X.

PhylomeDB

Q8WXF3.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

Bgee

Q8WXF3.

CleanEx

HS_RLN3.

Genevestigator

Q8WXF3.

GermOnline

ENSG00000171136. Homo sapiens.

Family and domain databases

InterPro

IPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]

Pfam

PF00049. Insulin. 1 hit.
[Graphical view]

PRINTS

PR00276. INSULINFAMLY.

SMART

SM00078. IlGF. 1 hit.
[Graphical view]

SUPFAM

SSF56994. Insulin-like. 1 hit.

PROSITE

PS00262. INSULIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8WXF3.

GenomeRNAi

117579.

NextBio

80214.

SOURCE

Search...

Entry information

Entry name

REL3_HUMAN

Accession

Primary (citable) accession number: Q8WXF3
Secondary accession number(s): Q6UXW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2003
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.