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Protein

Relaxin-3

Gene

RLN3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 (GPCR135) and relaxin-3 receptor-2 (GPCR142).

Keywords - Molecular functioni

Hormone

Enzyme and pathway databases

ReactomeiREACT_19231. G alpha (i) signalling events.
REACT_19327. G alpha (s) signalling events.
REACT_21314. Relaxin receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Relaxin-3
Alternative name(s):
Insulin-like peptide INSL7
Short name:
Insulin-like peptide 7
Prorelaxin H3
Cleaved into the following 2 chains:
Gene namesi
Name:RLN3
Synonyms:INSL7, RXN3, ZINS4
ORF Names:UNQ6188/PRO20213
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:17135. RLN3.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34401.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 PublicationAdd
BLAST
Peptidei26 – 5227Relaxin-3 B chainBy similarityPRO_0000016082Add
BLAST
Propeptidei55 – 11864Connecting peptideBy similarityPRO_0000016083Add
BLAST
Peptidei119 – 14224Relaxin-3 A chainBy similarityPRO_0000016084Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi35 ↔ 129Interchain (between B and A chains)1 Publication
Disulfide bondi47 ↔ 142Interchain (between B and A chains)1 Publication
Disulfide bondi128 ↔ 1331 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiQ8WXF3.
PRIDEiQ8WXF3.

PTM databases

PhosphoSiteiQ8WXF3.

Expressioni

Gene expression databases

BgeeiQ8WXF3.
CleanExiHS_RLN3.
ExpressionAtlasiQ8WXF3. baseline.
GenevestigatoriQ8WXF3.

Organism-specific databases

HPAiHPA043731.

Interactioni

Subunit structurei

Heterodimer of a B chain and an A chain linked by two disulfide bonds.

Binary interactionsi

WithEntry#Exp.IntActNotes
RXFP4Q8TDU99EBI-9519546,EBI-9519524

Protein-protein interaction databases

BioGridi125590. 3 interactions.
IntActiQ8WXF3. 1 interaction.
STRINGi9606.ENSP00000307040.

Structurei

Secondary structure

1
142
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi29 – 324Combined sources
Helixi37 – 4711Combined sources
Helixi120 – 13011Combined sources
Helixi135 – 1395Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FHWNMR-A119-142[»]
B26-52[»]
2K1VNMR-B26-52[»]
ProteinModelPortaliQ8WXF3.
SMRiQ8WXF3. Positions 26-52.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8WXF3.

Family & Domainsi

Sequence similaritiesi

Belongs to the insulin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG45013.
GeneTreeiENSGT00510000048203.
HOGENOMiHOG000111888.
HOVERGENiHBG045048.
InParanoidiQ8WXF3.
KOiK05255.
OMAiEARASPY.
OrthoDBiEOG7H1JNG.
PhylomeDBiQ8WXF3.
TreeFamiTF333404.

Family and domain databases

InterProiIPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR00276. INSULINFAMLY.
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 2 hits.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8WXF3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARYMLLLLL AVWVLTGELW PGAEARAAPY GVRLCGREFI RAVIFTCGGS
60 70 80 90 100
RWRRSDILAH EAMGDTFPDA DADEDSLAGE LDEAMGSSEW LALTKSPQAF
110 120 130 140
YRGRPSWQGT PGVLRGSRDV LAGLSSSCCK WGCSKSEISS LC
Length:142
Mass (Da):15,451
Last modified:March 1, 2002 - v1
Checksum:i23A3E095034B31E4
GO

Sequence cautioni

The sequence AAQ88548.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF447451 mRNA. Translation: AAL40345.1.
AB076563 mRNA. Translation: BAC53758.1.
AY358181 mRNA. Translation: AAQ88548.1. Different initiation.
CCDSiCCDS12302.1.
RefSeqiNP_543140.1. NM_080864.2.
UniGeneiHs.352155.

Genome annotation databases

EnsembliENST00000431365; ENSP00000397415; ENSG00000171136.
GeneIDi117579.
KEGGihsa:117579.
UCSCiuc002mxw.1. human.

Polymorphism databases

DMDMi37999891.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF447451 mRNA. Translation: AAL40345.1.
AB076563 mRNA. Translation: BAC53758.1.
AY358181 mRNA. Translation: AAQ88548.1. Different initiation.
CCDSiCCDS12302.1.
RefSeqiNP_543140.1. NM_080864.2.
UniGeneiHs.352155.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FHWNMR-A119-142[»]
B26-52[»]
2K1VNMR-B26-52[»]
ProteinModelPortaliQ8WXF3.
SMRiQ8WXF3. Positions 26-52.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125590. 3 interactions.
IntActiQ8WXF3. 1 interaction.
STRINGi9606.ENSP00000307040.

PTM databases

PhosphoSiteiQ8WXF3.

Polymorphism databases

DMDMi37999891.

Proteomic databases

PaxDbiQ8WXF3.
PRIDEiQ8WXF3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000431365; ENSP00000397415; ENSG00000171136.
GeneIDi117579.
KEGGihsa:117579.
UCSCiuc002mxw.1. human.

Organism-specific databases

CTDi117579.
GeneCardsiGC19P014137.
HGNCiHGNC:17135. RLN3.
HPAiHPA043731.
MIMi606855. gene.
neXtProtiNX_Q8WXF3.
PharmGKBiPA34401.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG45013.
GeneTreeiENSGT00510000048203.
HOGENOMiHOG000111888.
HOVERGENiHBG045048.
InParanoidiQ8WXF3.
KOiK05255.
OMAiEARASPY.
OrthoDBiEOG7H1JNG.
PhylomeDBiQ8WXF3.
TreeFamiTF333404.

Enzyme and pathway databases

ReactomeiREACT_19231. G alpha (i) signalling events.
REACT_19327. G alpha (s) signalling events.
REACT_21314. Relaxin receptors.

Miscellaneous databases

EvolutionaryTraceiQ8WXF3.
GenomeRNAii117579.
NextBioi80214.
PROiQ8WXF3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8WXF3.
CleanExiHS_RLN3.
ExpressionAtlasiQ8WXF3. baseline.
GenevestigatoriQ8WXF3.

Family and domain databases

InterProiIPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR00276. INSULINFAMLY.
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 2 hits.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Homo sapiens insulin homolog polypeptide."
    Holloway J.L., Lok S., Jaspers S.R.
    Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 26-34 AND 119-127.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "H3 relaxin is a specific ligand for LGR7 and activates the receptor by interacting with both the ectodomain and the exoloop 2."
    Sudo S., Kumagai J., Nishi S., Layfield S., Ferraro T., Bathgate R.A.D., Hsueh A.J.W.
    J. Biol. Chem. 278:7855-7862(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LGR7.
  5. "Identification of relaxin-3/INSL7 as an endogenous ligand for the orphan G-protein coupled receptor GPCR135."
    Liu C., Eriste E., Sutton S., Chen J., Roland B., Kuei C., Farmer N., Joernvall H., Sillard R., Lovenberg T.W.
    J. Biol. Chem. 278:50754-50764(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GPCR135.
  6. Cited for: INTERACTION WITH GPCR142.
  7. "Solution structure and novel insights into the determinants of the receptor specificity of human relaxin-3."
    Rosengren K.J., Lin F., Bathgate R.A., Tregear G.W., Daly N.L., Wade J.D., Craik D.J.
    J. Biol. Chem. 281:5845-5851(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 26-142, DISULFIDE BONDS.

Entry informationi

Entry nameiREL3_HUMAN
AccessioniPrimary (citable) accession number: Q8WXF3
Secondary accession number(s): Q6UXW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: March 1, 2002
Last modified: January 7, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.