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Protein

Paraspeckle component 1

Gene

PSPC1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity). Together with NONO, required for the formation of nuclear paraspeckles.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

SIGNORiQ8WXF1.

Names & Taxonomyi

Protein namesi
Recommended name:
Paraspeckle component 1
Alternative name(s):
Paraspeckle protein 1
Gene namesi
Name:PSPC1
Synonyms:PSP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

HGNCiHGNC:20320. PSPC1.

Subcellular locationi

  • Nucleusnucleolus
  • Nucleus matrix By similarity
  • Cytoplasm By similarity
  • Nucleus speckle

  • Note: In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in an RNA-dependent manner. May cycles between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi119F → A: Abolishes accumulation in paraspeckles, but not in perinucleolar caps; when associated with A-121; A-198 and A-200. 1 Publication1
Mutagenesisi121F → A: Abolishes accumulation in paraspeckles, but not in perinucleolar caps; when associated with A-119; A-198 and A-200. 1 Publication1
Mutagenesisi198K → A: Abolishes accumulation in paraspeckles, but not in perinucleolar caps; when associated with A-119; A-121 and A-200. 1 Publication1
Mutagenesisi200F → A: Abolishes accumulation in paraspeckles, but not in perinucleolar caps; when associated with A-119; A-121 and A-198. 1 Publication1
Mutagenesisi275Y → A: Abolishes interaction with NONO and localization in nuclear paraspeckles; when associated with A-279. 1 Publication1
Mutagenesisi279W → A: Abolishes interaction with NONO and localization in nuclear paraspeckles; when associated with A-275. 1 Publication1

Organism-specific databases

DisGeNETi55269.
OpenTargetsiENSG00000121390.
PharmGKBiPA134968603.

Polymorphism and mutation databases

BioMutaiPSPC1.
DMDMi74762636.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002975401 – 523Paraspeckle component 1Add BLAST523

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei409PhosphoserineCombined sources1
Modified residuei473PhosphoserineCombined sources1
Modified residuei477PhosphoserineCombined sources1
Modified residuei507Omega-N-methylarginineCombined sources1
Modified residuei509PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ8WXF1.
MaxQBiQ8WXF1.
PaxDbiQ8WXF1.
PeptideAtlasiQ8WXF1.
PRIDEiQ8WXF1.

PTM databases

iPTMnetiQ8WXF1.
PhosphoSitePlusiQ8WXF1.
SwissPalmiQ8WXF1.

Expressioni

Tissue specificityi

Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.1 Publication

Gene expression databases

BgeeiENSG00000121390.
CleanExiHS_PSPC1.
ExpressionAtlasiQ8WXF1. baseline and differential.
GenevisibleiQ8WXF1. HS.

Organism-specific databases

HPAiHPA038904.

Interactioni

Subunit structurei

Forms heterodimers with NONO; this involves formation of a coiled coil domain by helices from both proteins. Found in a RNP complex with CAT2 transcribed nuclear RNA (CTN-RNA). Interaction with NONO is required for its targeting to paraspeckles and perinucleolar caps. Interacts with SFPQ (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
KRT31Q153233EBI-1392258,EBI-948001
NONOQ1523311EBI-1392258,EBI-350527
NONOQ15233-23EBI-1392258,EBI-10203843

Protein-protein interaction databases

BioGridi120558. 43 interactors.
DIPiDIP-39028N.
IntActiQ8WXF1. 20 interactors.
MINTiMINT-1395719.
STRINGi9606.ENSP00000343966.

Structurei

Secondary structure

1523
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi80 – 82Combined sources3
Beta strandi83 – 88Combined sources6
Helixi95 – 101Combined sources7
Helixi102 – 105Combined sources4
Beta strandi109 – 114Combined sources6
Turni115 – 118Combined sources4
Beta strandi119 – 123Combined sources5
Helixi127 – 137Combined sources11
Beta strandi148 – 151Combined sources4
Beta strandi155 – 162Combined sources8
Helixi169 – 176Combined sources8
Helixi177 – 179Combined sources3
Beta strandi182 – 190Combined sources9
Beta strandi195 – 205Combined sources11
Helixi206 – 218Combined sources13
Beta strandi221 – 226Combined sources6
Beta strandi231 – 234Combined sources4
Beta strandi238 – 242Combined sources5
Helixi246 – 248Combined sources3
Helixi253 – 259Combined sources7
Beta strandi263 – 265Combined sources3
Helixi271 – 318Combined sources48

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SDEX-ray1.90A61-320[»]
ProteinModelPortaliQ8WXF1.
SMRiQ8WXF1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 154RRM 1PROSITE-ProRule annotationAdd BLAST73
Domaini156 – 237RRM 2PROSITE-ProRule annotationAdd BLAST82

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 358Sufficient for paraspeckles localizationAdd BLAST234
Regioni231 – 358Sufficient for perinucleolar caps localization and interaction with NONO1 PublicationAdd BLAST128

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili283 – 377Sequence analysisAdd BLAST95

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi394 – 516Gly-richAdd BLAST123

Sequence similaritiesi

Belongs to the PSPC family.Curated
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0115. Eukaryota.
ENOG410XQA0. LUCA.
GeneTreeiENSGT00390000005004.
HOGENOMiHOG000231095.
HOVERGENiHBG009801.
InParanoidiQ8WXF1.
OMAiMDNREQE.
OrthoDBiEOG091G09T7.
PhylomeDBiQ8WXF1.
TreeFamiTF315795.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012975. NOPS.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF08075. NOPS. 1 hit.
PF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WXF1-1) [UniParc]FASTAAdd to basket
Also known as: PSP1-alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMLRGNLKQV RIEKNPARLR ALESAVGESE PAAAAAMALA LAGEPAPPAP
60 70 80 90 100
APPEDHPDEE MGFTIDIKSF LKPGEKTYTQ RCRLFVGNLP TDITEEDFKR
110 120 130 140 150
LFERYGEPSE VFINRDRGFG FIRLESRTLA EIAKAELDGT ILKSRPLRIR
160 170 180 190 200
FATHGAALTV KNLSPVVSNE LLEQAFSQFG PVEKAVVVVD DRGRATGKGF
210 220 230 240 250
VEFAAKPPAR KALERCGDGA FLLTTTPRPV IVEPMEQFDD EDGLPEKLMQ
260 270 280 290 300
KTQQYHKERE QPPRFAQPGT FEFEYASRWK ALDEMEKQQR EQVDRNIREA
310 320 330 340 350
KEKLEAEMEA ARHEHQLMLM RQDLMRRQEE LRRLEELRNQ ELQKRKQIQL
360 370 380 390 400
RHEEEHRRRE EEMIRHREQE ELRRQQEGFK PNYMENREQE MRMGDMGPRG
410 420 430 440 450
AINMGDAFSP APAGNQGPPP MMGMNMNNRA TIPGPPMGPG PAMGPEGAAN
460 470 480 490 500
MGTPMMPDNG AVHNDRFPQG PPSQMGSPMG SRTGSETPQA PMSGVGPVSG
510 520
GPGGFGRGSQ GGNFEGPNKR RRY
Length:523
Mass (Da):58,744
Last modified:March 1, 2002 - v1
Checksum:i3A8C44079D64BBF0
GO
Isoform 2 (identifier: Q8WXF1-2) [UniParc]FASTAAdd to basket
Also known as: PSP1-beta

The sequence of this isoform differs from the canonical sequence as follows:
     387-393: REQEMRM → GDKRKCG
     394-523: Missing.

Show »
Length:393
Mass (Da):45,571
Checksum:i67F1A7EBF93050B2
GO

Sequence cautioni

The sequence BAA91924 differs from that shown. Reason: Erroneous termination at position 14. Translated as Lys.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_027274387 – 393REQEMRM → GDKRKCG in isoform 2. 5 Publications7
Alternative sequenceiVSP_027275394 – 523Missing in isoform 2. 5 PublicationsAdd BLAST130

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448795 mRNA. Translation: AAL59601.1.
AF449627 mRNA. Translation: AAL59602.1.
AK001817 mRNA. Translation: BAA91924.1. Sequence problems.
AL354808 Genomic DNA. Translation: CAI41004.1.
AL354808 Genomic DNA. Translation: CAI41005.1.
CH471075 Genomic DNA. Translation: EAX08232.1.
BC014184 mRNA. Translation: AAH14184.2.
CR457272 mRNA. Translation: CAG33553.1.
AL834505 mRNA. Translation: CAD39162.1.
CCDSiCCDS41870.1. [Q8WXF1-1]
RefSeqiNP_001035879.1. NM_001042414.2. [Q8WXF1-1]
XP_006719907.1. XM_006719844.3. [Q8WXF1-1]
XP_016876139.1. XM_017020650.1. [Q8WXF1-2]
UniGeneiHs.213198.

Genome annotation databases

EnsembliENST00000338910; ENSP00000343966; ENSG00000121390. [Q8WXF1-1]
ENST00000471658; ENSP00000436038; ENSG00000121390. [Q8WXF1-2]
ENST00000492741; ENSP00000435921; ENSG00000121390. [Q8WXF1-2]
ENST00000619300; ENSP00000481916; ENSG00000121390. [Q8WXF1-1]
GeneIDi55269.
KEGGihsa:55269.
UCSCiuc021rgx.2. human. [Q8WXF1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448795 mRNA. Translation: AAL59601.1.
AF449627 mRNA. Translation: AAL59602.1.
AK001817 mRNA. Translation: BAA91924.1. Sequence problems.
AL354808 Genomic DNA. Translation: CAI41004.1.
AL354808 Genomic DNA. Translation: CAI41005.1.
CH471075 Genomic DNA. Translation: EAX08232.1.
BC014184 mRNA. Translation: AAH14184.2.
CR457272 mRNA. Translation: CAG33553.1.
AL834505 mRNA. Translation: CAD39162.1.
CCDSiCCDS41870.1. [Q8WXF1-1]
RefSeqiNP_001035879.1. NM_001042414.2. [Q8WXF1-1]
XP_006719907.1. XM_006719844.3. [Q8WXF1-1]
XP_016876139.1. XM_017020650.1. [Q8WXF1-2]
UniGeneiHs.213198.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SDEX-ray1.90A61-320[»]
ProteinModelPortaliQ8WXF1.
SMRiQ8WXF1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120558. 43 interactors.
DIPiDIP-39028N.
IntActiQ8WXF1. 20 interactors.
MINTiMINT-1395719.
STRINGi9606.ENSP00000343966.

PTM databases

iPTMnetiQ8WXF1.
PhosphoSitePlusiQ8WXF1.
SwissPalmiQ8WXF1.

Polymorphism and mutation databases

BioMutaiPSPC1.
DMDMi74762636.

Proteomic databases

EPDiQ8WXF1.
MaxQBiQ8WXF1.
PaxDbiQ8WXF1.
PeptideAtlasiQ8WXF1.
PRIDEiQ8WXF1.

Protocols and materials databases

DNASUi55269.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000338910; ENSP00000343966; ENSG00000121390. [Q8WXF1-1]
ENST00000471658; ENSP00000436038; ENSG00000121390. [Q8WXF1-2]
ENST00000492741; ENSP00000435921; ENSG00000121390. [Q8WXF1-2]
ENST00000619300; ENSP00000481916; ENSG00000121390. [Q8WXF1-1]
GeneIDi55269.
KEGGihsa:55269.
UCSCiuc021rgx.2. human. [Q8WXF1-1]

Organism-specific databases

CTDi55269.
DisGeNETi55269.
GeneCardsiPSPC1.
H-InvDBHIX0011177.
HGNCiHGNC:20320. PSPC1.
HPAiHPA038904.
MIMi612408. gene.
neXtProtiNX_Q8WXF1.
OpenTargetsiENSG00000121390.
PharmGKBiPA134968603.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0115. Eukaryota.
ENOG410XQA0. LUCA.
GeneTreeiENSGT00390000005004.
HOGENOMiHOG000231095.
HOVERGENiHBG009801.
InParanoidiQ8WXF1.
OMAiMDNREQE.
OrthoDBiEOG091G09T7.
PhylomeDBiQ8WXF1.
TreeFamiTF315795.

Enzyme and pathway databases

SIGNORiQ8WXF1.

Miscellaneous databases

ChiTaRSiPSPC1. human.
GenomeRNAii55269.
PROiQ8WXF1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000121390.
CleanExiHS_PSPC1.
ExpressionAtlasiQ8WXF1. baseline and differential.
GenevisibleiQ8WXF1. HS.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012975. NOPS.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF08075. NOPS. 1 hit.
PF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSPC1_HUMAN
AccessioniPrimary (citable) accession number: Q8WXF1
Secondary accession number(s): Q5JTQ3
, Q8NCZ9, Q8WXE8, Q9NV36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.