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Q8WXF1 (PSPC1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Paraspeckle component 1
Alternative name(s):
Paraspeckle protein 1
Gene names
Name:PSPC1
Synonyms:PSP1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length523 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line By similarity. Binds to poly(A), poly(G) and poly(U) RNA homopolymers By similarity. Together with NONO, required for the formation of nuclear paraspeckles. Ref.13

Subunit structure

Forms heterodimers with NONO; this involves formation of a coiled coil domain by helices from both proteins. Found in a RNP complex with CAT2 transcribed nuclear RNA (CTN-RNA). Interaction with NONO is required for its targeting to paraspeckles and perinucleolar caps. Interacts with SFPQ By similarity. Ref.8

Subcellular location

Nucleusnucleolus. Nucleus matrix By similarity. Cytoplasm By similarity. Nucleus speckle. Note: In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in an RNA-dependent manner. May cycles between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps. Ref.1 Ref.8 Ref.13

Tissue specificity

Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart. Ref.1

Sequence similarities

Belongs to the PSPC family.

Contains 2 RRM (RNA recognition motif) domains.

Sequence caution

The sequence BAA91924.1 differs from that shown. Reason: Erroneous termination at position 14. Translated as Lys.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

NONOQ152336EBI-1392258,EBI-350527

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8WXF1-1)

Also known as: PSP1-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8WXF1-2)

Also known as: PSP1-beta;

The sequence of this isoform differs from the canonical sequence as follows:
     387-393: REQEMRM → GDKRKCG
     394-523: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 523523Paraspeckle component 1
PRO_0000297540

Regions

Domain82 – 15473RRM 1
Domain156 – 23782RRM 2
Region125 – 358234Sufficient for paraspeckles localization
Region231 – 358128Sufficient for perinucleolar caps localization and interaction with NONO
Coiled coil283 – 37795 Potential
Compositional bias394 – 516123Gly-rich

Amino acid modifications

Modified residue11N-acetylmethionine Ref.10
Modified residue4091Phosphoserine Ref.11
Modified residue4731Phosphoserine Ref.11
Modified residue4771Phosphoserine Ref.11
Modified residue5091Phosphoserine Ref.9

Natural variations

Alternative sequence387 – 3937REQEMRM → GDKRKCG in isoform 2.
VSP_027274
Alternative sequence394 – 523130Missing in isoform 2.
VSP_027275

Experimental info

Mutagenesis1191F → A: Abolishes accumulation in paraspeckles, but not in perinucleolar caps; when associated with A-121; A-198 and A-200. Ref.8
Mutagenesis1211F → A: Abolishes accumulation in paraspeckles, but not in perinucleolar caps; when associated with A-119; A-198 and A-200. Ref.8
Mutagenesis1981K → A: Abolishes accumulation in paraspeckles, but not in perinucleolar caps; when associated with A-119; A-121 and A-200. Ref.8
Mutagenesis2001F → A: Abolishes accumulation in paraspeckles, but not in perinucleolar caps; when associated with A-119; A-121 and A-198. Ref.8
Mutagenesis2751Y → A: Abolishes interaction with NONO and localization in nuclear paraspeckles; when associated with A-279. Ref.13
Mutagenesis2791W → A: Abolishes interaction with NONO and localization in nuclear paraspeckles; when associated with A-275. Ref.13

Secondary structure

....................................... 523
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (PSP1-alpha) [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 3A8C44079D64BBF0

FASTA52358,744
        10         20         30         40         50         60 
MMLRGNLKQV RIEKNPARLR ALESAVGESE PAAAAAMALA LAGEPAPPAP APPEDHPDEE 

        70         80         90        100        110        120 
MGFTIDIKSF LKPGEKTYTQ RCRLFVGNLP TDITEEDFKR LFERYGEPSE VFINRDRGFG 

       130        140        150        160        170        180 
FIRLESRTLA EIAKAELDGT ILKSRPLRIR FATHGAALTV KNLSPVVSNE LLEQAFSQFG 

       190        200        210        220        230        240 
PVEKAVVVVD DRGRATGKGF VEFAAKPPAR KALERCGDGA FLLTTTPRPV IVEPMEQFDD 

       250        260        270        280        290        300 
EDGLPEKLMQ KTQQYHKERE QPPRFAQPGT FEFEYASRWK ALDEMEKQQR EQVDRNIREA 

       310        320        330        340        350        360 
KEKLEAEMEA ARHEHQLMLM RQDLMRRQEE LRRLEELRNQ ELQKRKQIQL RHEEEHRRRE 

       370        380        390        400        410        420 
EEMIRHREQE ELRRQQEGFK PNYMENREQE MRMGDMGPRG AINMGDAFSP APAGNQGPPP 

       430        440        450        460        470        480 
MMGMNMNNRA TIPGPPMGPG PAMGPEGAAN MGTPMMPDNG AVHNDRFPQG PPSQMGSPMG 

       490        500        510        520 
SRTGSETPQA PMSGVGPVSG GPGGFGRGSQ GGNFEGPNKR RRY 

« Hide

Isoform 2 (PSP1-beta) [UniParc].

Checksum: 67F1A7EBF93050B2
Show »

FASTA39345,571

References

« Hide 'large scale' references
[1]"Paraspeckles: a novel nuclear domain."
Fox A.H., Lam Y.W., Leung A.K.L., Lyon C.E., Andersen J., Mann M., Lamond A.I.
Curr. Biol. 12:13-25(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF 21-83; 105-124; 135-143; 185-194; 199-210; 252-259; 265-289; 327-345; 352-357; 366-374 AND 483-507, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Placenta.
[3]"The DNA sequence and analysis of human chromosome 13."
Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Skin.
[6]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 37-523 (ISOFORM 2).
[7]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 192-523 (ISOFORM 2).
Tissue: Kidney.
[8]"P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner."
Fox A.H., Bond C.S., Lamond A.I.
Mol. Biol. Cell 16:5304-5315(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, INTERACTION WITH NONO, MUTAGENESIS OF PHE-119; PHE-121; LYS-198 AND PHE-200, SUBCELLULAR LOCATION.
[9]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-509, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic kidney.
[10]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-409; SER-473 AND SER-477, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation."
Passon D.M., Lee M., Rackham O., Stanley W.A., Sadowska A., Filipovska A., Fox A.H., Bond C.S.
Proc. Natl. Acad. Sci. U.S.A. 109:4846-4850(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 61-320 IN COMPLEX WITH NONO, FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF TYR-275 AND TRP-279.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF448795 mRNA. Translation: AAL59601.1.
AF449627 mRNA. Translation: AAL59602.1.
AK001817 mRNA. Translation: BAA91924.1. Sequence problems.
AL354808 Genomic DNA. Translation: CAI41004.1.
AL354808 Genomic DNA. Translation: CAI41005.1.
CH471075 Genomic DNA. Translation: EAX08232.1.
BC014184 mRNA. Translation: AAH14184.2.
CR457272 mRNA. Translation: CAG33553.1.
AL834505 mRNA. Translation: CAD39162.1.
CCDSCCDS41870.1. [Q8WXF1-1]
RefSeqNP_001035879.1. NM_001042414.2. [Q8WXF1-1]
XP_006719907.1. XM_006719844.1. [Q8WXF1-1]
UniGeneHs.213198.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3SDEX-ray1.90A61-320[»]
ProteinModelPortalQ8WXF1.
SMRQ8WXF1. Positions 66-320.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120558. 20 interactions.
DIPDIP-39028N.
IntActQ8WXF1. 9 interactions.
MINTMINT-1395719.
STRING9606.ENSP00000343966.

PTM databases

PhosphoSiteQ8WXF1.

Polymorphism databases

DMDM74762636.

Proteomic databases

MaxQBQ8WXF1.
PaxDbQ8WXF1.
PRIDEQ8WXF1.

Protocols and materials databases

DNASU55269.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000338910; ENSP00000343966; ENSG00000121390. [Q8WXF1-1]
ENST00000471658; ENSP00000436038; ENSG00000121390. [Q8WXF1-2]
ENST00000492741; ENSP00000435921; ENSG00000121390. [Q8WXF1-2]
GeneID55269.
KEGGhsa:55269.
UCSCuc021rgx.1. human. [Q8WXF1-1]

Organism-specific databases

CTD55269.
GeneCardsGC13M020249.
H-InvDBHIX0011177.
HGNCHGNC:20320. PSPC1.
HPAHPA038904.
MIM612408. gene.
neXtProtNX_Q8WXF1.
PharmGKBPA134968603.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG298586.
HOGENOMHOG000231095.
HOVERGENHBG009801.
InParanoidQ8WXF1.
OMAMDNREQE.
OrthoDBEOG7327P0.
PhylomeDBQ8WXF1.
TreeFamTF315795.

Gene expression databases

BgeeQ8WXF1.
CleanExHS_PSPC1.
GenevestigatorQ8WXF1.

Family and domain databases

Gene3D3.30.70.330. 2 hits.
InterProIPR012975. NOPS.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF08075. NOPS. 1 hit.
PF00076. RRM_1. 2 hits.
[Graphical view]
SMARTSM00360. RRM. 2 hits.
[Graphical view]
PROSITEPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPSPC1. human.
GenomeRNAi55269.
NextBio59380.
PROQ8WXF1.
SOURCESearch...

Entry information

Entry namePSPC1_HUMAN
AccessionPrimary (citable) accession number: Q8WXF1
Secondary accession number(s): Q5JTQ3 expand/collapse secondary AC list , Q8NCZ9, Q8WXE8, Q9NV36
Entry history
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 13

Human chromosome 13: entries, gene names and cross-references to MIM