Q8WXE9 (STON2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Stonin-2

Alternative name(s):
Stoned B

Gene names

Name:	STON2
Synonyms:	STN2, STNB

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	905 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adapter protein involved in endocytic machinery. May be involved in the vesicle recycling. May facilitate clathrin-coated vesicle uncoating. Ref.1 Ref.5
Subunit structure	Interacts with the second C2 domain of synaptotagmins SYT1 and SYT2. Interacts with EPS15, EPS15R and ITSN1. Interacts indirectly with the AP-2 adapter complex. Ref.1 Ref.5
Subcellular location	Cytoplasm. Membrane. Note: Some fraction is membrane-associated. Ref.5
Tissue specificity	Ubiquitous. Ref.5
Domain	The Asn-Pro-Phe (NPF) motifs, which are found in proteins involved in the endocytic pathway, mediate the interaction with the EH domain of SYT1, SYT2, EPS15, EPS15R and ITSN1.
Sequence similarities	Belongs to the Stoned B family. Contains 1 MHD (mu homology) domain. Contains 1 SHD (stonin homology) domain.
Sequence caution	The sequence AAK57558.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAK57558.1 differs from that shown. Reason: Intron retention. The sequence AAK76362.1 differs from that shown. Reason: Frameshift at position 122.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Polymorphism
Domain	Repeat
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW intracellular protein transport Inferred from electronic annotation. Source: InterPro regulation of endocytosis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	clathrin adaptor complex Inferred from electronic annotation. Source: InterPro nucleolus Inferred from direct assay. Source: HPA
Molecular function	protein binding Inferred from physical interaction Ref.1. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Syt1	P21707	2	EBI-539742,EBI-458098	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 905

905

Stonin-2

PRO_0000185732

Regions

Domain

427 – 560

134

SHD

Domain

565 – 874

310

MHD

Motif

313 – 315

3

NPF 1

Motif

329 – 331

3

NPF 2

Amino acid modifications

Modified residue

300

1

Phosphothreonine Ref.6

Modified residue

302

1

Phosphoserine Ref.6

Natural variations

Natural variant

307

1

S → P. Ref.1 Ref.4
Corresponds to variant rs3813535 [ dbSNP | Ensembl ].

VAR_020182

Natural variant

646

1

R → H.
Corresponds to variant rs34323725 [ dbSNP | Ensembl ].

VAR_046643

Natural variant

694

1

T → A.
Corresponds to variant rs35689202 [ dbSNP | Ensembl ].

VAR_046644

Natural variant

851

1

S → A.
Corresponds to variant rs2241621 [ dbSNP | Ensembl ].

VAR_021912

Experimental info

Mutagenesis

738

1

W → A: Reduces interaction with SYT1. Ref.1

Mutagenesis

740

1

K → A: Reduces interaction with SYT1. Ref.1

Sequence conflict

121 – 122

2

TA → NS in AAK76362. Ref.1

Secondary structure

1

.

.

.

.

.

.

905

Helix Strand Turn

Details...

Beta strand

314 – 317

4

Turn

318 – 320

3

Helix

334 – 337

4

Sequences

	Sequence		Length	Mass (Da)
	Q8WXE9 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 59F5810DEC540CA3	FASTA	905	101,165
	10 20 30 40 50 60 MTTLDHVIAT HQSEWVSFNE EPPFPAHSQG GTEEHLPGLS SSPDQSESSS GENHVVDGGS 70 80 90 100 110 120 QDHSHSEQDD SSEKMGLISE AASPPGSPEQ PPPDLASAIS NWVQFEDDTP WASTSPPHQE 130 140 150 160 170 180 TAETALPLTM PCWTCPSFDS LGRCPLTSES SWTTHSEDTS SPSFGCSYTD LQLINAEEQT 190 200 210 220 230 240 SGQASGADST DNSSSLQEDE EVEMEAISWQ ASSPAMNGHP APPVTSARFP SWVTFDDNEV 250 260 270 280 290 300 SCPLPPVTSP LKPNTPPSAS VIPDVPYNSM GSFKKRDRPK STLMNFSKVQ KLDISSLNRT 310 320 330 340 350 360 PSVTEASPWR ATNPFLNETL QDVQPSPINP FSAFFEEQER RSQNSSISST TGKSQRDSLI 370 380 390 400 410 420 VIYQDAISFD DSSKTQSHSD AVEKLKQLQI DDPDHFGSAT LPDDDPVAWI ELDAHPPGSA 430 440 450 460 470 480 RSQPRDGWPM MLRIPEKKNI MSSRHWGPIF VKLTDTGYLQ LYYEQGLEKP FREFKLEICH 490 500 510 520 530 540 EISEPRLQNY DENGRIHSLR IDRVTYKEKK KYQPKPAVAH TAEREQVIKL GTTNYDDFLS 550 560 570 580 590 600 FIHAVQDRLM DLPVLSMDLS TVGLNYLEEE ITVDVRDEFS GIVSKGDNQI LQHHVLTRIH 610 620 630 640 650 660 ILSFLSGLAE CRLGLNDILV KGNEIVLRQD IMPTTTTKWI KLHECRFHGC VDEDVFHNSR 670 680 690 700 710 720 VILFNPLDAC RFELMRFRTV FAEKTLPFTL RTATSVNGAE VEVQSWLRMS TGFSANRDPL 730 740 750 760 770 780 TQVPCENVMI RYPVPSEWVK NFRRESVLGE KSLKAKVNRG ASFGSTSVSG SEPVMRVTLG 790 800 810 820 830 840 TAKYEHAFNS IVWRINRLPD KNSASGHPHC FFCHLELGSD REVPSRFANH VNVEFSMPTT 850 860 870 880 890 900 SASKASVRSI SVEDKTDVRK WVNYSAHYSY QVALGSIWLM LPTPFVHPTT LPLLFLLAML TMFAW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human stoned B interacts with AP-2 and synaptotagmin and facilitates clathrin-coated vesicle uncoating." Walther K., Krauss M., Diril M.K., Lemke S., Ricotta D., Hoening S., Kaiser S., Haucke V. EMBO Rep. 2:634-640(2001) [PubMed: 11454741] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH SYT1 AND WITH THE AP-2 COMPLEX, MUTAGENESIS OF TRP-738 AND LYS-740, VARIANT PRO-307. Tissue: Brain.
[2]	Erratum Walther K., Krauss M., Diril M.K., Lemke S., Ricotta D., Hoening S., Kaiser S., Haucke V. EMBO Rep. 3:197-197(2002)
[3]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed: 12508121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-307. Tissue: Brain.
[5]	"Stonin 2: an adaptor-like protein that interacts with components of the endocytic machinery." Martina J.A., Bonangelino C.J., Aguilar R.C., Bonifacino J.S. J. Cell Biol. 153:1111-1120(2001) [PubMed: 11381094] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 191-905, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH EPS15; EPS15R; ITSN1; SYT1 AND SYT2.
[6]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-300 AND SER-302, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Structure of the Eps15-stonin2 complex provides a molecular explanation for EH-domain ligand specificity." Rumpf J., Simon B., Jung N., Maritzen T., Haucke V., Sattler M., Groemping Y. EMBO J. 27:558-569(2008) [PubMed: 18200045] [Abstract] Cited for: STRUCTURE BY NMR OF 121-215 IN COMPLEX WITH EPS15.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF380833 mRNA. Translation: AAK76362.1. Frameshift.
AF449430 mRNA. Translation: AAL47008.1.
AL121769 Genomic DNA. No translation available.
AL136040 Genomic DNA. No translation available.
BC069389 mRNA. Translation: AAH69389.1.
BC117493 mRNA. Translation: AAI17494.1.
AF255309 mRNA. Translation: AAK57558.1. Sequence problems.

IPI

IPI00103521.

RefSeq

NP_149095.2. NM_033104.2.

UniGene

Hs.14248.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JXC	NMR	-	B	301-340	[»]

ProteinModelPortal

Q8WXE9.

SMR

Q8WXE9. Positions 301-340, 557-872.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8WXE9. 4 interactions.

MINT

MINT-6179820.

STRING

Q8WXE9.

PTM databases

PhosphoSite

Q8WXE9.

Polymorphism databases

DMDM

34098614.

Proteomic databases

PRIDE

Q8WXE9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000267540; ENSP00000267540; ENSG00000140022.

GeneID

85439.

KEGG

hsa:85439.

UCSC

uc001xvl.1. human.
uc001xvm.1. human.

Organism-specific databases

CTD

85439.

GeneCards

GC14M081736.

H-InvDB

HIX0011863.

HGNC

HGNC:30652. STON2.

HPA

HPA003086.

MIM

608467. gene.

neXtProt

NX_Q8WXE9.

PharmGKB

PA143485624.

GenAtlas

Search...

Phylogenomic databases

GeneTree

ENSGT00530000063152.

HOVERGEN

HBG097637.

InParanoid

Q8WXE9.

OMA

FFCHLEL.

OrthoDB

EOG4XSKP6.

PhylomeDB

Q8WXE9.

Gene expression databases

ArrayExpress

Q8WXE9.

Bgee

Q8WXE9.

CleanEx

HS_STON2.

Genevestigator

Q8WXE9.

GermOnline

ENSG00000140022. Homo sapiens.

Family and domain databases

InterPro

IPR008968. Clathrin_mu_C.
IPR012320. SHD.
IPR017110. Stonin.
IPR022699. Stonin2_N.
[Graphical view]

Pfam

PF00928. Adap_comp_sub. 1 hit.
PF12016. Stonin2_N. 1 hit.
[Graphical view]

PIRSF

PIRSF037099. Stonin. 1 hit.

SUPFAM

SSF49447. AP50. 1 hit.

PROSITE

PS51072. MHD. 1 hit.
PS51070. SHD. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

76012.

SOURCE

Search...

Entry information

Entry name

STON2_HUMAN

Accession

Primary (citable) accession number: Q8WXE9
Secondary accession number(s): Q17R24 , Q6NT47, Q96RI7, Q96RU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.