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Protein

ATR-interacting protein

Gene

ATRIP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.1 Publication

GO - Molecular functioni

  • K63-linked polyubiquitin binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

ReactomeiR-HSA-176187. Activation of ATR in response to replication stress.
R-HSA-5685938. HDR through Single Strand Annealing (SSA).
R-HSA-5693607. Processing of DNA double-strand break ends.
R-HSA-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-HSA-6783310. Fanconi Anemia Pathway.
R-HSA-6804756. Regulation of TP53 Activity through Phosphorylation.
R-HSA-69473. G2/M DNA damage checkpoint.
SIGNORiQ8WXE1.

Names & Taxonomyi

Protein namesi
Recommended name:
ATR-interacting protein
Alternative name(s):
ATM and Rad3-related-interacting protein
Gene namesi
Name:ATRIP
Synonyms:AGS1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:33499. ATRIP.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi769 – 7702EE → AA: Abolishes interaction with ATR and its recruitment to sites of DNA damage. 1 Publication
Mutagenesisi774 – 7752DD → AA: Abolishes interaction with ATR and its recruitment to sites of DNA damage. 1 Publication

Organism-specific databases

MalaCardsiATRIP.
Orphaneti808. Seckel syndrome.
PharmGKBiPA162377290.

Polymorphism and mutation databases

DMDMi48428109.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 791791ATR-interacting proteinPRO_0000064740Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki96 – 96Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei518 – 5181PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated by ATR.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8WXE1.
MaxQBiQ8WXE1.
PaxDbiQ8WXE1.
PeptideAtlasiQ8WXE1.
PRIDEiQ8WXE1.

PTM databases

iPTMnetiQ8WXE1.
PhosphoSiteiQ8WXE1.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSG00000164053.
CleanExiHS_ATRIP.
ExpressionAtlasiQ8WXE1. baseline and differential.
GenevisibleiQ8WXE1. HS.

Organism-specific databases

HPAiCAB033109.

Interactioni

Subunit structurei

Interacts with ATR (By similarity). Heterodimer with ATR. The heterodimer binds the RPA complex and is then recruited to single-stranded DNA. Interacts with CEP164 (via N-terminus). Interacts with CINP.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
C1orf94Q6P1W53EBI-747353,EBI-946029
CCDC28BQ9BUN53EBI-747353,EBI-10299032
CDC23Q9UJX23EBI-747353,EBI-396137
CDK9P507503EBI-747353,EBI-1383449
CINPQ9BW663EBI-747353,EBI-739784
FAM156AQ8NDB63EBI-747353,EBI-749727
FAM208BQ5VWN6-23EBI-747353,EBI-10172380
LNX1Q8TBB13EBI-747353,EBI-739832
METTL21AQ8WXB13EBI-747353,EBI-8652459
MID2Q9UJV3-23EBI-747353,EBI-10172526
MOSP005403EBI-747353,EBI-1757866
MX2P205923EBI-747353,EBI-10200618
PCTK1Q9BRL43EBI-747353,EBI-10296950
POLR1CO151603EBI-747353,EBI-1055079
SCG2P135213EBI-747353,EBI-947132
SPRY2O435973EBI-747353,EBI-742487

GO - Molecular functioni

  • K63-linked polyubiquitin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi313463. 45 interactions.
DIPiDIP-46495N.
IntActiQ8WXE1. 23 interactions.
MINTiMINT-5009350.
STRINGi9606.ENSP00000323099.

Structurei

Secondary structure

1
791
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi57 – 637Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IGKX-ray1.75C/D237-243[»]
4NB3X-ray1.35C/D54-67[»]
ProteinModelPortaliQ8WXE1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni118 – 15639Interaction with CINPAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili108 – 217110Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi769 – 7768EEXXXDL motif

Domaini

The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.1 Publication

Sequence similaritiesi

Belongs to the ATRIP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IHIV. Eukaryota.
ENOG41107QS. LUCA.
GeneTreeiENSGT00390000012850.
HOGENOMiHOG000273897.
HOVERGENiHBG050618.
InParanoidiQ8WXE1.
KOiK10905.
OMAiGSNCQCN.
OrthoDBiEOG091G03DF.
PhylomeDBiQ8WXE1.
TreeFamiTF324417.

Family and domain databases

InterProiIPR033349. ATRIP.
[Graphical view]
PANTHERiPTHR28594. PTHR28594. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WXE1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGTSAPGSK RRSEPPAPRP GPPPGTGHPP SKRARGFSAA AAPDPDDPFG
60 70 80 90 100
AHGDFTADDL EELDTLASQA LSQCPAAARD VSSDHKVHRL LDGMSKNPSG
110 120 130 140 150
KNRETVPIKD NFELEVLQAQ YKELKEKMKV MEEEVLIKNG EIKILRDSLH
160 170 180 190 200
QTESVLEEQR RSHFLLEQEK TQALSDKEKE FSKKLQSLQS ELQFKDAEMN
210 220 230 240 250
ELRTKLQTSE RANKLAAPSV SHVSPRKNPS VVIKPEACSP QFGKTSFPTK
260 270 280 290 300
ESFSANMSLP HPCQTESGYK PLVGREDSKP HSLRGDSIKQ EEAQKSFVDS
310 320 330 340 350
WRQRSNTQGS ILINLLLKQP LIPGSSLSLC HLLSSSSESP AGTPLQPPGF
360 370 380 390 400
GSTLAGMSGL RTTGSYDGSF SLSALREAQN LAFTGLNLVA RNECSRDGDP
410 420 430 440 450
AEGGRRAFPL CQLPGAVHFL PLVQFFIGLH CQALQDLAAA KRSGAPGDSP
460 470 480 490 500
THSSCVSSGV ETNPEDSVCI LEGFSVTALS ILQHLVCHSG AVVSLLLSGV
510 520 530 540 550
GADSAAGEGN RSLVHRLSDG DMTSALRGVA DDQGQHPLLK MLLHLLAFSS
560 570 580 590 600
AATGHLQASV LTQCLKVLVK LAENTSCDFL PRFQCVFQVL PKCLSPETPL
610 620 630 640 650
PSVLLAVELL SLLADHDQLA PQLCSHSEGC LLLLLYMYIT SRPDRVALET
660 670 680 690 700
QWLQLEQEVV WLLAKLGVQS PLPPVTGSNC QCNVEVVRAL TVMLHRQWLT
710 720 730 740 750
VRRAGGPPRT DQQRRTVRCL RDTVLLLHGL SQKDKLFMMH CVEVLHQFDQ
760 770 780 790
VMPGVSMLIR GLPDVTDCEE AALDDLCAAE TDVEDPEVEC G
Length:791
Mass (Da):85,838
Last modified:March 1, 2002 - v1
Checksum:i58981602F7961756
GO
Isoform 2 (identifier: Q8WXE1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     661-687: Missing.

Show »
Length:764
Mass (Da):83,003
Checksum:i94E7E31A6BCAAC1D
GO
Isoform 3 (identifier: Q8WXE1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-93: Missing.

Note: No experimental confirmation available.
Show »
Length:698
Mass (Da):76,336
Checksum:i417F5811408F2799
GO
Isoform 4 (identifier: Q8WXE1-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-127: Missing.

Show »
Length:664
Mass (Da):72,362
Checksum:i460E7E097BCA1C30
GO

Sequence cautioni

The sequence BAB14029 differs from that shown. Reason: Frameshift at position 650. Curated
The sequence BAB14029 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAF84257 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti280 – 2801P → L in AAH14153 (PubMed:15489334).Curated
Sequence conflicti343 – 3431T → A in BAF84257 (PubMed:14702039).Curated
Sequence conflicti492 – 4921V → I in BAF84257 (PubMed:14702039).Curated
Sequence conflicti647 – 6471A → T in BAG62254 (PubMed:14702039).Curated
Sequence conflicti683 – 6831N → D in BAF84257 (PubMed:14702039).Curated
Sequence conflicti687 – 6871V → I in AAH30597 (PubMed:15489334).Curated
Sequence conflicti712 – 7121Q → K in BAF84257 (PubMed:14702039).Curated
Sequence conflicti777 – 7771C → G in BAF84257 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti125 – 1251K → Q.
Corresponds to variant rs11925638 [ dbSNP | Ensembl ].
VAR_050683
Natural varianti240 – 2401P → L.
Corresponds to variant rs35240314 [ dbSNP | Ensembl ].
VAR_050684

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 127127Missing in isoform 4. 1 PublicationVSP_047011Add
BLAST
Alternative sequencei1 – 9393Missing in isoform 3. 1 PublicationVSP_010501Add
BLAST
Alternative sequencei661 – 68727Missing in isoform 2. 2 PublicationsVSP_010504Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF451323 mRNA. Translation: AAL38042.1.
AK022405 mRNA. Translation: BAB14029.1. Sequence problems.
AK291568 mRNA. Translation: BAF84257.1. Different initiation.
AK291829 mRNA. Translation: BAF84518.1.
AK315075 mRNA. Translation: BAG37544.1.
AK300548 mRNA. Translation: BAG62254.1.
AC104448 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW64876.1.
CH471055 Genomic DNA. Translation: EAW64878.1.
BC014153 mRNA. Translation: AAH14153.2.
BC020563 mRNA. Translation: AAH20563.1.
BC030597 mRNA. Translation: AAH30597.1.
AL832917 mRNA. Translation: CAH10621.1.
CCDSiCCDS2767.1. [Q8WXE1-2]
CCDS2768.1. [Q8WXE1-1]
CCDS59449.1. [Q8WXE1-3]
CCDS59450.1. [Q8WXE1-5]
RefSeqiNP_001257951.1. NM_001271022.1. [Q8WXE1-5]
NP_001257952.1. NM_001271023.1. [Q8WXE1-3]
NP_115542.2. NM_032166.3. [Q8WXE1-2]
NP_569055.1. NM_130384.2. [Q8WXE1-1]
UniGeneiHs.694840.

Genome annotation databases

EnsembliENST00000320211; ENSP00000323099; ENSG00000164053. [Q8WXE1-1]
ENST00000346691; ENSP00000302338; ENSG00000164053. [Q8WXE1-2]
ENST00000412052; ENSP00000400930; ENSG00000164053. [Q8WXE1-3]
GeneIDi84126.
KEGGihsa:84126.
UCSCiuc003ctf.3. human. [Q8WXE1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF451323 mRNA. Translation: AAL38042.1.
AK022405 mRNA. Translation: BAB14029.1. Sequence problems.
AK291568 mRNA. Translation: BAF84257.1. Different initiation.
AK291829 mRNA. Translation: BAF84518.1.
AK315075 mRNA. Translation: BAG37544.1.
AK300548 mRNA. Translation: BAG62254.1.
AC104448 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW64876.1.
CH471055 Genomic DNA. Translation: EAW64878.1.
BC014153 mRNA. Translation: AAH14153.2.
BC020563 mRNA. Translation: AAH20563.1.
BC030597 mRNA. Translation: AAH30597.1.
AL832917 mRNA. Translation: CAH10621.1.
CCDSiCCDS2767.1. [Q8WXE1-2]
CCDS2768.1. [Q8WXE1-1]
CCDS59449.1. [Q8WXE1-3]
CCDS59450.1. [Q8WXE1-5]
RefSeqiNP_001257951.1. NM_001271022.1. [Q8WXE1-5]
NP_001257952.1. NM_001271023.1. [Q8WXE1-3]
NP_115542.2. NM_032166.3. [Q8WXE1-2]
NP_569055.1. NM_130384.2. [Q8WXE1-1]
UniGeneiHs.694840.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IGKX-ray1.75C/D237-243[»]
4NB3X-ray1.35C/D54-67[»]
ProteinModelPortaliQ8WXE1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi313463. 45 interactions.
DIPiDIP-46495N.
IntActiQ8WXE1. 23 interactions.
MINTiMINT-5009350.
STRINGi9606.ENSP00000323099.

PTM databases

iPTMnetiQ8WXE1.
PhosphoSiteiQ8WXE1.

Polymorphism and mutation databases

DMDMi48428109.

Proteomic databases

EPDiQ8WXE1.
MaxQBiQ8WXE1.
PaxDbiQ8WXE1.
PeptideAtlasiQ8WXE1.
PRIDEiQ8WXE1.

Protocols and materials databases

DNASUi84126.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000320211; ENSP00000323099; ENSG00000164053. [Q8WXE1-1]
ENST00000346691; ENSP00000302338; ENSG00000164053. [Q8WXE1-2]
ENST00000412052; ENSP00000400930; ENSG00000164053. [Q8WXE1-3]
GeneIDi84126.
KEGGihsa:84126.
UCSCiuc003ctf.3. human. [Q8WXE1-1]

Organism-specific databases

CTDi84126.
GeneCardsiATRIP.
GeneReviewsiATRIP.
HGNCiHGNC:33499. ATRIP.
HPAiCAB033109.
MalaCardsiATRIP.
MIMi606605. gene.
neXtProtiNX_Q8WXE1.
Orphaneti808. Seckel syndrome.
PharmGKBiPA162377290.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHIV. Eukaryota.
ENOG41107QS. LUCA.
GeneTreeiENSGT00390000012850.
HOGENOMiHOG000273897.
HOVERGENiHBG050618.
InParanoidiQ8WXE1.
KOiK10905.
OMAiGSNCQCN.
OrthoDBiEOG091G03DF.
PhylomeDBiQ8WXE1.
TreeFamiTF324417.

Enzyme and pathway databases

ReactomeiR-HSA-176187. Activation of ATR in response to replication stress.
R-HSA-5685938. HDR through Single Strand Annealing (SSA).
R-HSA-5693607. Processing of DNA double-strand break ends.
R-HSA-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-HSA-6783310. Fanconi Anemia Pathway.
R-HSA-6804756. Regulation of TP53 Activity through Phosphorylation.
R-HSA-69473. G2/M DNA damage checkpoint.
SIGNORiQ8WXE1.

Miscellaneous databases

GenomeRNAii84126.
PROiQ8WXE1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000164053.
CleanExiHS_ATRIP.
ExpressionAtlasiQ8WXE1. baseline and differential.
GenevisibleiQ8WXE1. HS.

Family and domain databases

InterProiIPR033349. ATRIP.
[Graphical view]
PANTHERiPTHR28594. PTHR28594. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiATRIP_HUMAN
AccessioniPrimary (citable) accession number: Q8WXE1
Secondary accession number(s): A8K6A3
, A8K714, B2RCE7, B4DU92, B5MEB7, Q69YK9, Q8NHQ2, Q8WUG7, Q96CL3, Q9HA30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: March 1, 2002
Last modified: September 7, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

The gene for this protein is either identical to or adjacent to that of TREX1. Some of the mRNAs that encode ATRIP also encode TREX1 in another reading frame.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.