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Q8WXE0 (CSKI2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Caskin-2
Alternative name(s):
CASK-interacting protein 2
Gene names
Name:CASKIN2
Synonyms:KIAA1139
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1202 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

May not bind CASK.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Contains 6 ANK repeats.

Contains 2 SAM (sterile alpha motif) domains.

Contains 1 SH3 domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainANK repeat
Repeat
SH3 domain
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from sequence or structural similarity Ref.1. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8WXE0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8WXE0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12021202Caskin-2
PRO_0000066983

Regions

Repeat48 – 7730ANK 1
Repeat81 – 11030ANK 2
Repeat114 – 14330ANK 3
Repeat147 – 17630ANK 4
Repeat188 – 21730ANK 5
Repeat220 – 24930ANK 6
Domain281 – 34767SH3
Domain489 – 55264SAM 1
Domain558 – 62265SAM 2
Compositional bias685 – 1133449Pro-rich

Amino acid modifications

Modified residue3931Phosphoserine Ref.6 Ref.8
Modified residue3961Phosphoserine Ref.6 Ref.7 Ref.8
Modified residue4031Phosphoserine By similarity
Modified residue4711Phosphoserine Ref.6 Ref.7
Modified residue8581Phosphoserine Ref.6
Modified residue8921Phosphoserine Ref.6

Natural variations

Alternative sequence1 – 8282Missing in isoform 2.
VSP_040568
Natural variant8911E → G. Ref.1 Ref.2 Ref.4 Ref.5 Ref.6
Corresponds to variant rs7503373 [ dbSNP | Ensembl ].
VAR_060244

Experimental info

Sequence conflict6401A → G in BAH14307. Ref.2
Sequence conflict10931L → P in BAH14307. Ref.2

Secondary structure

..................................... 1202
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 18, 2010. Version 2.
Checksum: 881E6BAEEBDF3DC5

FASTA1,202126,783
        10         20         30         40         50         60 
MGREQDLILA VKNGDVTGVQ KLVAKVKATK TKLLGSTKRL NVNYQDADGF SALHHAALGG 

        70         80         90        100        110        120 
SLELIALLLE AQATVDIKDS NGMRPLHYAA WQGRLEPVRL LLRASAAVNA ASLDGQIPLH 

       130        140        150        160        170        180 
LAAQYGHYEV SEMLLQHQSN PCLVNKAKKT PLDLACEFGR LKVAQLLLNS HLCVALLEGE 

       190        200        210        220        230        240 
AKDPCDPNYT TPLHLAAKNG HREVIRQLLR AGIEINRQTK TGTALHEAAL YGKTEVVRLL 

       250        260        270        280        290        300 
LEGGVDVNIR NTYNQTALDI VNQFTTSQAS REIKQLLREA SGILKVRALK DFWNLHDPTA 

       310        320        330        340        350        360 
LNVRAGDVIT VLEQHPDGRW KGHIHESQRG TDRIGYFPPG IVEVVSKRVG IPAARLPSAP 

       370        380        390        400        410        420 
TPLRPGFSRT PQPPAEEPPH PLTYSQLPRV GLSPDSPAGD RNSVGSEGSV GSIRSAGSGQ 

       430        440        450        460        470        480 
SSEGTNGHGP GLLIENAQPL PSAGEDQVLP GLHPPSLADN LSHRPLANCR SGEQIFTQDV 

       490        500        510        520        530        540 
RPEQLLEGKD AQAIHNWLSE FQLEGYTAHF LQAGYDVPTI SRMTPEDLTA IGVTKPGHRK 

       550        560        570        580        590        600 
KIASEIAQLS IAEWLPSYIP TDLLEWLCAL GLPQYHKQLV SSGYDSMGLV ADLTWEELQE 

       610        620        630        640        650        660 
IGVNKLGHQK KLMLGVKRLA ELRRGLLQGE ALSEGGRRLA KGPELMAIEG LENGEGPATA 

       670        680        690        700        710        720 
GPRLLTFQGS ELSPELQAAM AGGGPEPLPL PPARSPSQES IGARSRGSGH SQEQPAPQPS 

       730        740        750        760        770        780 
GGDPSPPQER NLPEGTERPP KLCSSLPGQG PPPYVFMYPQ GSPSSPAPGP PPGAPWAFSY 

       790        800        810        820        830        840 
LAGPPATPPD PPRPKRRSHS LSRPGPTEGD AEGEAEGPVG STLGSYATLT RRPGRSALVR 

       850        860        870        880        890        900 
TSPSVTPTPA RGTPRSQSFA LRARRKGPPP PPPKRLSSVS GPSPEPPPLD ESPGPKEGAT 

       910        920        930        940        950        960 
GPRRRTLSEP AGPSEPPGPP APAGPASDTE EEEPGPEGTP PSRGSSGEGL PFAEEGNLTI 

       970        980        990       1000       1010       1020 
KQRPKPAGPP PRETPVPPGL DFNLTESDTV KRRPKCRERE PLQTALLAFG VASATPGPAA 

      1030       1040       1050       1060       1070       1080 
PLPSPTPGES PPASSLPQPE PSSLPAQGVP TPLAPSPAMQ PPVPPCPGPG LESSAASRWN 

      1090       1100       1110       1120       1130       1140 
GETEPPAAPA ALLKVPGAGT APKPVSVACT QLAFSGPKLA PRLGPRPVPP PRPESTGTVG 

      1150       1160       1170       1180       1190       1200 
PGQAQQRLEQ TSSSLAAALR AAEKSIGTKE QEGTPSASTK HILDDISTMF DALADQLDAM 


LD 

« Hide

Isoform 2 [UniParc].

Checksum: F120B5C26DAD308B
Show »

FASTA1,120118,157

References

« Hide 'large scale' references
[1]"CASK participates in alternative tripartite complexes in which Mint 1 competes for binding with Caskin 1, a novel CASK-binding protein."
Tabuchi K., Biederer T., Butz S., Suedhof T.C.
J. Neurosci. 22:4264-4273(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT GLY-891.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT GLY-891.
Tissue: Placenta.
[3]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLY-891.
Tissue: Testis.
[5]"Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain."
Hirosawa M., Nagase T., Ishikawa K., Kikuno R., Nomura N., Ohara O.
DNA Res. 6:329-336(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 79-1202 (ISOFORM 1), VARIANT GLY-891.
Tissue: Brain.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-393; SER-396; SER-471; SER-858 AND SER-892, VARIANT [LARGE SCALE ANALYSIS] GLY-891, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-396 AND SER-471, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[8]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-393 AND SER-396, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Error tolerant NMR backbone resonance assignment and automated structure generation."
Alipanahi B., Gao X., Karakoc E., Li S.C., Balbach F., Feng G., Donaldson L., Li M.
J. Bioinform. Comput. Biol. 9:15-41(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 284-348.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF451976 mRNA. Translation: AAL49757.1.
AK300354 mRNA. Translation: BAG62095.1.
AK315936 mRNA. Translation: BAH14307.1.
AC100787 Genomic DNA. No translation available.
BC066643 mRNA. Translation: AAH66643.1.
AB032965 mRNA. Translation: BAA86453.1.
CCDSCCDS11723.1. [Q8WXE0-1]
CCDS45775.1. [Q8WXE0-2]
RefSeqNP_001136115.1. NM_001142643.2. [Q8WXE0-2]
NP_065804.2. NM_020753.4. [Q8WXE0-1]
UniGeneHs.274408.
Hs.745460.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2KE9NMR-A284-348[»]
4IS7X-ray2.75A483-633[»]
ProteinModelPortalQ8WXE0.
SMRQ8WXE0. Positions 282-348, 488-627.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121577. 3 interactions.
IntActQ8WXE0. 1 interaction.
MINTMINT-6768531.
STRING9606.ENSP00000325355.

PTM databases

PhosphoSiteQ8WXE0.

Polymorphism databases

DMDM296434467.

Proteomic databases

MaxQBQ8WXE0.
PaxDbQ8WXE0.
PRIDEQ8WXE0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000321617; ENSP00000325355; ENSG00000177303. [Q8WXE0-1]
ENST00000433559; ENSP00000406963; ENSG00000177303. [Q8WXE0-2]
GeneID57513.
KEGGhsa:57513.
UCSCuc002joc.4. human. [Q8WXE0-1]

Organism-specific databases

CTD57513.
GeneCardsGC17M073496.
H-InvDBHIX0020291.
HGNCHGNC:18200. CASKIN2.
HPAHPA055836.
MIM612185. gene.
neXtProtNX_Q8WXE0.
PharmGKBPA134944093.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0666.
HOGENOMHOG000049168.
HOVERGENHBG051133.
InParanoidQ8WXE0.
OMAPYVFMYP.
OrthoDBEOG7V49XK.
PhylomeDBQ8WXE0.
TreeFamTF320582.

Gene expression databases

ArrayExpressQ8WXE0.
BgeeQ8WXE0.
CleanExHS_CASKIN2.
GenevestigatorQ8WXE0.

Family and domain databases

Gene3D1.10.150.50. 2 hits.
1.25.40.20. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR027001. Caskin/Ankyrin_rpt_con_pro.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERPTHR24177. PTHR24177. 1 hit.
PfamPF12796. Ank_2. 2 hits.
PF00536. SAM_1. 2 hits.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSPR01415. ANKYRIN.
SMARTSM00248. ANK. 6 hits.
SM00454. SAM. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF47769. SSF47769. 2 hits.
SSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS50105. SAM_DOMAIN. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi57513.
NextBio63878.
PROQ8WXE0.
SOURCESearch...

Entry information

Entry nameCSKI2_HUMAN
AccessionPrimary (citable) accession number: Q8WXE0
Secondary accession number(s): B4DTT3 expand/collapse secondary AC list , B7Z9H1, Q7LG69, Q9ULT1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM