Q8WXE0 (CSKI2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Caskin-2

Alternative name(s):
CASK-interacting protein 2

Gene names

Name:	CASKIN2
Synonyms:	KIAA1139

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1202 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	May not bind CASK.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 6 ANK repeats. Contains 2 SAM (sterile alpha motif) domains. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	ANK repeat Repeat SH3 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from sequence or structural similarity Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8WXE0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8WXE0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1202

1202

Caskin-2

PRO_0000066983

Regions

Repeat

48 – 77

30

ANK 1

Repeat

81 – 110

30

ANK 2

Repeat

114 – 143

30

ANK 3

Repeat

147 – 176

30

ANK 4

Repeat

188 – 217

30

ANK 5

Repeat

220 – 249

30

ANK 6

Domain

281 – 347

67

SH3

Domain

489 – 552

64

SAM 1

Domain

558 – 622

65

SAM 2

Compositional bias

685 – 1133

449

Pro-rich

Amino acid modifications

Modified residue

253

1

Phosphotyrosine By similarity

Modified residue

393

1

Phosphoserine Ref.6 Ref.8

Modified residue

396

1

Phosphoserine Ref.6 Ref.7 Ref.8

Modified residue

403

1

Phosphoserine By similarity

Modified residue

409

1

Phosphoserine By similarity

Modified residue

471

1

Phosphoserine Ref.6 Ref.7

Modified residue

858

1

Phosphoserine Ref.6

Modified residue

892

1

Phosphoserine Ref.6

Natural variations

Alternative sequence

1 – 82

82

Missing in isoform 2.

VSP_040568

Natural variant

891

1

E → G. Ref.1 Ref.2 Ref.4 Ref.5 Ref.6
Corresponds to variant rs7503373 [ dbSNP | Ensembl ].

VAR_060244

Experimental info

Sequence conflict

640

1

A → G in BAH14307. Ref.2

Sequence conflict

1093

1

L → P in BAH14307. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

1202

Helix Strand Turn

Details...

Beta strand

284 – 290

7

Beta strand

308 – 312

5

Beta strand

320 – 324

5

Beta strand

335 – 337

3

Helix

339 – 341

3

Beta strand

342 – 345

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: 881E6BAEEBDF3DC5	FASTA	1,202	126,783
	10 20 30 40 50 60 MGREQDLILA VKNGDVTGVQ KLVAKVKATK TKLLGSTKRL NVNYQDADGF SALHHAALGG 70 80 90 100 110 120 SLELIALLLE AQATVDIKDS NGMRPLHYAA WQGRLEPVRL LLRASAAVNA ASLDGQIPLH 130 140 150 160 170 180 LAAQYGHYEV SEMLLQHQSN PCLVNKAKKT PLDLACEFGR LKVAQLLLNS HLCVALLEGE 190 200 210 220 230 240 AKDPCDPNYT TPLHLAAKNG HREVIRQLLR AGIEINRQTK TGTALHEAAL YGKTEVVRLL 250 260 270 280 290 300 LEGGVDVNIR NTYNQTALDI VNQFTTSQAS REIKQLLREA SGILKVRALK DFWNLHDPTA 310 320 330 340 350 360 LNVRAGDVIT VLEQHPDGRW KGHIHESQRG TDRIGYFPPG IVEVVSKRVG IPAARLPSAP 370 380 390 400 410 420 TPLRPGFSRT PQPPAEEPPH PLTYSQLPRV GLSPDSPAGD RNSVGSEGSV GSIRSAGSGQ 430 440 450 460 470 480 SSEGTNGHGP GLLIENAQPL PSAGEDQVLP GLHPPSLADN LSHRPLANCR SGEQIFTQDV 490 500 510 520 530 540 RPEQLLEGKD AQAIHNWLSE FQLEGYTAHF LQAGYDVPTI SRMTPEDLTA IGVTKPGHRK 550 560 570 580 590 600 KIASEIAQLS IAEWLPSYIP TDLLEWLCAL GLPQYHKQLV SSGYDSMGLV ADLTWEELQE 610 620 630 640 650 660 IGVNKLGHQK KLMLGVKRLA ELRRGLLQGE ALSEGGRRLA KGPELMAIEG LENGEGPATA 670 680 690 700 710 720 GPRLLTFQGS ELSPELQAAM AGGGPEPLPL PPARSPSQES IGARSRGSGH SQEQPAPQPS 730 740 750 760 770 780 GGDPSPPQER NLPEGTERPP KLCSSLPGQG PPPYVFMYPQ GSPSSPAPGP PPGAPWAFSY 790 800 810 820 830 840 LAGPPATPPD PPRPKRRSHS LSRPGPTEGD AEGEAEGPVG STLGSYATLT RRPGRSALVR 850 860 870 880 890 900 TSPSVTPTPA RGTPRSQSFA LRARRKGPPP PPPKRLSSVS GPSPEPPPLD ESPGPKEGAT 910 920 930 940 950 960 GPRRRTLSEP AGPSEPPGPP APAGPASDTE EEEPGPEGTP PSRGSSGEGL PFAEEGNLTI 970 980 990 1000 1010 1020 KQRPKPAGPP PRETPVPPGL DFNLTESDTV KRRPKCRERE PLQTALLAFG VASATPGPAA 1030 1040 1050 1060 1070 1080 PLPSPTPGES PPASSLPQPE PSSLPAQGVP TPLAPSPAMQ PPVPPCPGPG LESSAASRWN 1090 1100 1110 1120 1130 1140 GETEPPAAPA ALLKVPGAGT APKPVSVACT QLAFSGPKLA PRLGPRPVPP PRPESTGTVG 1150 1160 1170 1180 1190 1200 PGQAQQRLEQ TSSSLAAALR AAEKSIGTKE QEGTPSASTK HILDDISTMF DALADQLDAM LD
	Isoform 2 [UniParc]. Checksum: F120B5C26DAD308B	FASTA	1,120	118,157
	10 20 30 40 50 60 MRPLHYAAWQ GRLEPVRLLL RASAAVNAAS LDGQIPLHLA AQYGHYEVSE MLLQHQSNPC 70 80 90 100 110 120 LVNKAKKTPL DLACEFGRLK VAQLLLNSHL CVALLEGEAK DPCDPNYTTP LHLAAKNGHR 130 140 150 160 170 180 EVIRQLLRAG IEINRQTKTG TALHEAALYG KTEVVRLLLE GGVDVNIRNT YNQTALDIVN 190 200 210 220 230 240 QFTTSQASRE IKQLLREASG ILKVRALKDF WNLHDPTALN VRAGDVITVL EQHPDGRWKG 250 260 270 280 290 300 HIHESQRGTD RIGYFPPGIV EVVSKRVGIP AARLPSAPTP LRPGFSRTPQ PPAEEPPHPL 310 320 330 340 350 360 TYSQLPRVGL SPDSPAGDRN SVGSEGSVGS IRSAGSGQSS EGTNGHGPGL LIENAQPLPS 370 380 390 400 410 420 AGEDQVLPGL HPPSLADNLS HRPLANCRSG EQIFTQDVRP EQLLEGKDAQ AIHNWLSEFQ 430 440 450 460 470 480 LEGYTAHFLQ AGYDVPTISR MTPEDLTAIG VTKPGHRKKI ASEIAQLSIA EWLPSYIPTD 490 500 510 520 530 540 LLEWLCALGL PQYHKQLVSS GYDSMGLVAD LTWEELQEIG VNKLGHQKKL MLGVKRLAEL 550 560 570 580 590 600 RRGLLQGEAL SEGGRRLAKG PELMAIEGLE NGEGPATAGP RLLTFQGSEL SPELQAAMAG 610 620 630 640 650 660 GGPEPLPLPP ARSPSQESIG ARSRGSGHSQ EQPAPQPSGG DPSPPQERNL PEGTERPPKL 670 680 690 700 710 720 CSSLPGQGPP PYVFMYPQGS PSSPAPGPPP GAPWAFSYLA GPPATPPDPP RPKRRSHSLS 730 740 750 760 770 780 RPGPTEGDAE GEAEGPVGST LGSYATLTRR PGRSALVRTS PSVTPTPARG TPRSQSFALR 790 800 810 820 830 840 ARRKGPPPPP PKRLSSVSGP SPEPPPLDES PGPKEGATGP RRRTLSEPAG PSEPPGPPAP 850 860 870 880 890 900 AGPASDTEEE EPGPEGTPPS RGSSGEGLPF AEEGNLTIKQ RPKPAGPPPR ETPVPPGLDF 910 920 930 940 950 960 NLTESDTVKR RPKCREREPL QTALLAFGVA SATPGPAAPL PSPTPGESPP ASSLPQPEPS 970 980 990 1000 1010 1020 SLPAQGVPTP LAPSPAMQPP VPPCPGPGLE SSAASRWNGE TEPPAAPAAL LKVPGAGTAP 1030 1040 1050 1060 1070 1080 KPVSVACTQL AFSGPKLAPR LGPRPVPPPR PESTGTVGPG QAQQRLEQTS SSLAAALRAA 1090 1100 1110 1120 EKSIGTKEQE GTPSASTKHI LDDISTMFDA LADQLDAMLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CASK participates in alternative tripartite complexes in which Mint 1 competes for binding with Caskin 1, a novel CASK-binding protein." Tabuchi K., Biederer T., Butz S., Suedhof T.C. J. Neurosci. 22:4264-4273(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT GLY-891.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT GLY-891. Tissue: Placenta.
[3]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLY-891. Tissue: Testis.
[5]	"Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain." Hirosawa M., Nagase T., Ishikawa K., Kikuno R., Nomura N., Ohara O. DNA Res. 6:329-336(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 79-1202 (ISOFORM 1), VARIANT GLY-891. Tissue: Brain.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-393; SER-396; SER-471; SER-858 AND SER-892, VARIANT [LARGE SCALE ANALYSIS] GLY-891, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-396 AND SER-471, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-393 AND SER-396, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Error tolerant NMR backbone resonance assignment and automated structure generation." Alipanahi B., Gao X., Karakoc E., Li S.C., Balbach F., Feng G., Donaldson L., Li M. J. Bioinform. Comput. Biol. 9:15-41(2011) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 284-348.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF451976 mRNA. Translation: AAL49757.1.
AK300354 mRNA. Translation: BAG62095.1.
AK315936 mRNA. Translation: BAH14307.1.
AC100787 Genomic DNA. No translation available.
BC066643 mRNA. Translation: AAH66643.1.
AB032965 mRNA. Translation: BAA86453.1.

IPI

IPI00103516.
IPI00916548.

RefSeq

NP_001136115.1. NM_001142643.2.
NP_065804.2. NM_020753.4.

UniGene

Hs.274408.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KE9	NMR	-	A	284-348	[»]

ProteinModelPortal

Q8WXE0.

SMR

Q8WXE0. Positions 4-348, 487-624.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8WXE0. 1 interaction.

MINT

MINT-6768531.

STRING

9606.ENSP00000325355.

PTM databases

PhosphoSite

Q8WXE0.

Polymorphism databases

DMDM

296434467.

Proteomic databases

PaxDb

Q8WXE0.

PRIDE

Q8WXE0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000321617; ENSP00000325355; ENSG00000177303.
ENST00000433559; ENSP00000406963; ENSG00000177303.

GeneID

57513.

KEGG

hsa:57513.

UCSC

uc002joc.3. human.

Organism-specific databases

CTD

57513.

GeneCards

GC17M073496.

H-InvDB

HIX0020291.

HGNC

HGNC:18200. CASKIN2.

MIM

612185. gene.

neXtProt

NX_Q8WXE0.

PharmGKB

PA134944093.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0666.

HOGENOM

HOG000049168.

HOVERGEN

HBG051133.

InParanoid

Q8WXE0.

OMA

PYVFMYP.

OrthoDB

EOG415GCP.

PhylomeDB

Q8WXE0.

Gene expression databases

Bgee

Q8WXE0.

CleanEx

HS_CASKIN2.

Genevestigator

Q8WXE0.

GermOnline

ENSG00000177303. Homo sapiens.

Family and domain databases

Gene3D

1.10.150.50. 2 hits.
1.25.40.20. 1 hit.

InterPro

IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR027013. Caskin.
IPR027001. Caskin/Ankyrin_rpt_con_pro.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]

PANTHER

PTHR24177. PTHR24177. 1 hit.
PTHR24177:SF0. PTHR24177:SF0. 1 hit.

Pfam

PF12796. Ank_2. 2 hits.
PF00536. SAM_1. 2 hits.
PF07653. SH3_2. 1 hit.
[Graphical view]

PRINTS

PR01415. ANKYRIN.

SMART

SM00248. ANK. 6 hits.
SM00454. SAM. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF48403. ANK. 1 hit.
SSF47769. SAM_homology. 2 hits.
SSF50044. SH3. 1 hit.

PROSITE

PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS50105. SAM_DOMAIN. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

57513.

NextBio

63878.

SOURCE

Search...

Entry information

Entry name

CSKI2_HUMAN

Accession

Primary (citable) accession number: Q8WXE0
Secondary accession number(s): B4DTT3 , B7Z9H1, Q7LG69, Q9ULT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.