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Q8WXD9 (CSKI1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Caskin-1
Alternative name(s):
CASK-interacting protein 1
Gene names
Name:CASKIN1
Synonyms:KIAA1306
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1431 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May link the scaffolding protein CASK to downstream intracellular effectors By similarity.

Subunit structure

Binds the CaM kinase domain of CASK. Forms a ternary complex with CASK and LIN7A, LIN7B or LIN7C. Competes with APBA1 that forms a similar complex with CASK and LIN7 proteins. The tripartite complex CASKIN1/CASK/LIN7(A/B/C) binds the cytoplasmic tail of NRXN1 By similarity. Polymerizes, via the tandem SAM domains, to form long, 8 nM wide fibers, upon which other proteins can assemble. Ref.3

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Contains 6 ANK repeats.

Contains 2 SAM (sterile alpha motif) domains.

Contains 1 SH3 domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   DomainANK repeat
Repeat
SH3 domain
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processsignal transduction

Inferred from sequence or structural similarity Ref.1. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity Ref.1. Source: UniProtKB

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 21763699. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CASKO149364EBI-970261,EBI-1215506

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14311431Caskin-1
PRO_0000066980

Regions

Repeat48 – 7730ANK 1
Repeat81 – 11030ANK 2
Repeat114 – 14330ANK 3
Repeat147 – 17630ANK 4
Repeat188 – 21730ANK 5
Repeat220 – 24930ANK 6
Domain281 – 34767SH3
Domain472 – 53564SAM 1
Domain541 – 60565SAM 2
Region375 – 46995CASK-binding By similarity
Compositional bias714 – 1365652Pro-rich

Amino acid modifications

Modified residue2531Phosphotyrosine By similarity
Modified residue12571Phosphoserine By similarity
Modified residue13641Phosphoserine By similarity

Experimental info

Sequence conflict2781R → P in BAA92544. Ref.2

Secondary structure

........................ 1431
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8WXD9 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: CDF60E68B81E884A

FASTA1,431149,814
        10         20         30         40         50         60 
MGKEQELVQA VKAEDVGTAQ RLLQRPRPGK AKLLGSTKKI NVNFQDPDGF SALHHAALNG 

        70         80         90        100        110        120 
NTELISLLLE AQAAVDIKDN KGMRPLHYAA WQGRKEPMKL VLKAGSAVNI PSDEGHIPLH 

       130        140        150        160        170        180 
LAAQHGHYDV SEMLLQHQSN PCMVDNSGKT PLDLACEFGR VGVVQLLLSS NMCAALLEPR 

       190        200        210        220        230        240 
PGDATDPNGT SPLHLAAKNG HIDIIRLLLQ AGIDINRQTK SGTALHEAAL CGKTEVVRLL 

       250        260        270        280        290        300 
LDSGINAHVR NTYSQTALDI VHQFTTSQAS REIKQLLREA SAALQVRATK DYCNNYDLTS 

       310        320        330        340        350        360 
LNVKAGDIIT VLEQHPDGRW KGCIHDNRTG NDRVGYFPSS LGEAIVKRAG SRAGTEPSLP 

       370        380        390        400        410        420 
QGSSSSGPSA PPEEIWVLRK PFAGGDRSGS ISGMAGGRGS GGHALHAGSE GVKLLATVLS 

       430        440        450        460        470        480 
QKSVSESGPG DSPAKPPEGS AGVARSQPPV AHAGQVYGEQ PPKKLEPASE GKSSEAVSQW 

       490        500        510        520        530        540 
LTAFQLQLYA PNFISAGYDL PTISRMTPED LTAIGVTKPG HRKKIAAEIS GLSIPDWLPE 

       550        560        570        580        590        600 
HKPANLAVWL SMIGLAQYYK VLVDNGYENI DFITDITWED LQEIGITKLG HQKKLMLAVR 

       610        620        630        640        650        660 
KLAELQKAEY AKYEGGPLRR KAPQSLEVMA IESPPPPEPT PADCQSPKMT TFQDSELSDE 

       670        680        690        700        710        720 
LQAAMTGPAE VGPTTEKPSS HLPPTPRATT RQDSSLGGRA RHMSSSQELL GDGPPGPSSP 

       730        740        750        760        770        780 
MSRSQEYLLD EGPAPGTPPR EARPGRHGHS IKRASVPPVP GKPRQVLPPG TSHFTPPQTP 

       790        800        810        820        830        840 
TKTRPGSPQA LGGPHGPAPA TAKVKPTPQL LPPTERPMSP RSLPQSPTHR GFAYVLPQPV 

       850        860        870        880        890        900 
EGEVGPAAPG PAPPPVPTAV PTLCLPPEAD AEPGRPKKRA HSLNRYAASD SEPERDELLV 

       910        920        930        940        950        960 
PAAAGPYATV QRRVGRSHSV RAPAGADKNV NRSQSFAVRP RKKGPPPPPP KRSSSALASA 

       970        980        990       1000       1010       1020 
NLADEPVPDA EPEDGLLGVR AQCRRASDLA GSVDTGSAGS VKSIAAMLEL SSIGGGGRAA 

      1030       1040       1050       1060       1070       1080 
RRPPEGHPTP RPASPEPGRV ATVLASVKHK EAIGPGGEVV NRRRTLSGPV TGLLATARRG 

      1090       1100       1110       1120       1130       1140 
PGESADPGPF VEDGTGRQRP RGPSKGEAGV EGPPLAKVEA SATLKRRIRA KQNQQENVKF 

      1150       1160       1170       1180       1190       1200 
ILTESDTVKR RPKAKEREAG PEPPPPLSVY HNGTGTVRRR PASEQAGPPE LPPPPPPAEP 

      1210       1220       1230       1240       1250       1260 
PPTDLAHLPP LPPPEGEARK PAKPPVSPKP VLTQPVPKLQ GSPTPTSKKV PLPGPGSPEV 

      1270       1280       1290       1300       1310       1320 
KRAHGTPPPV SPKPPPPPTA PKPVKAVAGL PSGSAGPSPA PSPARQPPAA LAKPPGTPPS 

      1330       1340       1350       1360       1370       1380 
LGASPAKPPS PGAPALHVPA KPPRAAAAAA AAAAAPPAPP EGASPGDSAR QKLEETSACL 

      1390       1400       1410       1420       1430 
AAALQAVEEK IRQEDAQGPR DSAAEKSTGS ILDDIGSMFD DLADQLDAML E 

« Hide

References

« Hide 'large scale' references
[1]"CASK participates in alternative tripartite complexes in which Mint 1 competes for binding with Caskin 1, a novel CASK-binding protein."
Tabuchi K., Biederer T., Butz S., Suedhof T.C.
J. Neurosci. 22:4264-4273(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 278-1431.
Tissue: Brain.
[3]"Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK."
Stafford R.L., Hinde E., Knight M.J., Pennella M.A., Ear J., Digman M.A., Gratton E., Bowie J.U.
Structure 19:1826-1836(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 470-605, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF451977 mRNA. Translation: AAL49758.1.
AB037727 mRNA. Translation: BAA92544.1.
CCDSCCDS42103.1.
RefSeqNP_065815.1. NM_020764.3.
UniGeneHs.643537.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3SEIX-ray2.40A/B470-605[»]
3SENX-ray3.10A/B/C/D470-613[»]
ProteinModelPortalQ8WXD9.
SMRQ8WXD9. Positions 19-348, 472-613.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121585. 1 interaction.
DIPDIP-34890N.
IntActQ8WXD9. 1 interaction.
STRING9606.ENSP00000345436.

PTM databases

PhosphoSiteQ8WXD9.

Polymorphism databases

DMDM61213003.

Proteomic databases

MaxQBQ8WXD9.
PaxDbQ8WXD9.
PRIDEQ8WXD9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000343516; ENSP00000345436; ENSG00000167971.
GeneID57524.
KEGGhsa:57524.
UCSCuc010bsg.1. human.

Organism-specific databases

CTD57524.
GeneCardsGC16M002227.
HGNCHGNC:20879. CASKIN1.
HPAHPA055990.
MIM612184. gene.
neXtProtNX_Q8WXD9.
PharmGKBPA134878267.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0666.
HOGENOMHOG000049168.
HOVERGENHBG051133.
InParanoidQ8WXD9.
OMAPKIQGSP.
OrthoDBEOG7V49XK.
PhylomeDBQ8WXD9.
TreeFamTF320582.

Gene expression databases

ArrayExpressQ8WXD9.
BgeeQ8WXD9.
CleanExHS_CASKIN1.
GenevestigatorQ8WXD9.

Family and domain databases

Gene3D1.10.150.50. 2 hits.
1.25.40.20. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR027013. Caskin-1.
IPR027001. Caskin/Ankyrin_rpt_con_pro.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERPTHR24177. PTHR24177. 1 hit.
PTHR24177:SF12. PTHR24177:SF12. 1 hit.
PfamPF12796. Ank_2. 2 hits.
PF00536. SAM_1. 2 hits.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSPR01415. ANKYRIN.
SMARTSM00248. ANK. 6 hits.
SM00454. SAM. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF47769. SSF47769. 2 hits.
SSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 6 hits.
PS50105. SAM_DOMAIN. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi57524.
NextBio63914.
PROQ8WXD9.
SOURCESearch...

Entry information

Entry nameCSKI1_HUMAN
AccessionPrimary (citable) accession number: Q8WXD9
Secondary accession number(s): Q9P2P0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM