Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein N-lysine methyltransferase METTL21A

Gene

METTL21A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein-lysine methyltransferase that selectively trimethylates residues in heat shock protein 70 (HSP70) family members. Contributes to the in vivo trimethylation of Lys residues in HSPA1 and HSPA8. In vitro methylates 'Lys-561' in HSPA1, 'Lys-564' in HSPA2, 'Lys-585' in HSPA5, 'Lys-563' in HSPA6 and 'Lys-561' in HSPA8.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei47S-adenosyl-L-methionine; via amide nitrogen1 Publication1
Binding sitei94S-adenosyl-L-methionine1 Publication1
Binding sitei125S-adenosyl-L-methionine; via amide nitrogen1 Publication1
Binding sitei143S-adenosyl-L-methionine; via carbonyl oxygen1 Publication1

GO - Molecular functioni

  • ATPase binding Source: BHF-UCL
  • Hsp70 protein binding Source: BHF-UCL
  • protein-lysine N-methyltransferase activity Source: UniProtKB

GO - Biological processi

  • peptidyl-lysine methylation Source: BHF-UCL
  • protein methylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciZFISH:ENSG00000144401-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein N-lysine methyltransferase METTL21A (EC:2.1.1.-)
Alternative name(s):
HSPA lysine methyltransferase
HSPA-KMT
Hepatocellular carcinoma-associated antigen 557b
Methyltransferase-like protein 21A
Gene namesi
Name:METTL21A
Synonyms:FAM119A, HCA557B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:30476. METTL21A.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi94D → A: Abolishes methyltransferase activity. 1 Publication1

Organism-specific databases

DisGeNETi151194.
OpenTargetsiENSG00000144401.
PharmGKBiPA145008433.

Chemistry databases

ChEMBLiCHEMBL3588741.

Polymorphism and mutation databases

BioMutaiMETTL21A.
DMDMi150382834.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002920331 – 218Protein N-lysine methyltransferase METTL21AAdd BLAST218

Proteomic databases

EPDiQ8WXB1.
MaxQBiQ8WXB1.
PaxDbiQ8WXB1.
PeptideAtlasiQ8WXB1.
PRIDEiQ8WXB1.

PTM databases

iPTMnetiQ8WXB1.
PhosphoSitePlusiQ8WXB1.

Expressioni

Gene expression databases

BgeeiENSG00000144401.
CleanExiHS_FAM119A.
ExpressionAtlasiQ8WXB1. baseline and differential.
GenevisibleiQ8WXB1. HS.

Organism-specific databases

HPAiHPA034712.

Interactioni

Subunit structurei

Interacts with heat shock protein 70 family members; at least some of these proteins are methylation substrates (PubMed:23349634 and PubMed:23921388).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ATRIPQ8WXE13EBI-8652459,EBI-747353
LNX1Q8TBB13EBI-8652459,EBI-739832
ZBED8Q8IZ133EBI-8652459,EBI-7186123

GO - Molecular functioni

  • ATPase binding Source: BHF-UCL
  • Hsp70 protein binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi127352. 20 interactors.
IntActiQ8WXB1. 6 interactors.
STRINGi9606.ENSP00000385481.

Structurei

Secondary structure

1218
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 18Combined sources3
Beta strandi19 – 26Combined sources8
Beta strandi29 – 34Combined sources6
Turni37 – 40Combined sources4
Helixi42 – 44Combined sources3
Helixi48 – 58Combined sources11
Beta strandi68 – 73Combined sources6
Helixi78 – 85Combined sources8
Beta strandi89 – 94Combined sources6
Helixi96 – 107Combined sources12
Helixi112 – 115Combined sources4
Beta strandi119 – 122Combined sources4
Helixi129 – 131Combined sources3
Beta strandi138 – 144Combined sources7
Helixi149 – 151Combined sources3
Helixi152 – 162Combined sources11
Beta strandi168 – 173Combined sources6
Helixi178 – 190Combined sources13
Beta strandi191 – 199Combined sources9
Turni200 – 203Combined sources4
Beta strandi204 – 212Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LECX-ray2.28A/B8-218[»]
ProteinModelPortaliQ8WXB1.
SMRiQ8WXB1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni73 – 75S-adenosyl-L-methionine binding1 Publication3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2793. Eukaryota.
ENOG4111IJ0. LUCA.
GeneTreeiENSGT00550000074572.
HOVERGENiHBG059353.
InParanoidiQ8WXB1.
PhylomeDBiQ8WXB1.
TreeFamiTF313206.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR019410. Methyltransf_16.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF10294. Methyltransf_16. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WXB1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALVPYEETT EFGLQKFHKP LATFSFANHT IQIRQDWRHL GVAAVVWDAA
60 70 80 90 100
IVLSTYLEMG AVELRGRSAV ELGAGTGLVG IVAALLGAHV TITDRKVALE
110 120 130 140 150
FLKSNVQANL PPHIQTKTVV KELTWGQNLG SFSPGEFDLI LGADIIYLEE
160 170 180 190 200
TFTDLLQTLE HLCSNHSVIL LACRIRYERD NNFLAMLERQ FTVRKVHYDP
210
EKDVHIYEAQ KRNQKEDL
Length:218
Mass (Da):24,600
Last modified:June 26, 2007 - v2
Checksum:i18E7E39A1CDCC56E
GO
Isoform 2 (identifier: Q8WXB1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     88-90: AHV → GGI
     91-218: Missing.

Show »
Length:90
Mass (Da):9,609
Checksum:i4A8C7FFF7CDB4C1C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33I → T in BAC04229 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_032935192T → I.3 PublicationsCorresponds to variant rs2551949dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02637988 – 90AHV → GGI in isoform 2. 1 Publication3
Alternative sequenceiVSP_02638091 – 218Missing in isoform 2. 1 PublicationAdd BLAST128

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF455817 mRNA. Translation: AAL66295.1.
AK093812 mRNA. Translation: BAC04229.1.
AC079767 Genomic DNA. Translation: AAX93175.1.
BC009462 mRNA. Translation: AAH09462.1.
BC033720 mRNA. Translation: AAH33720.1.
CCDSiCCDS2376.1. [Q8WXB1-1]
CCDS82562.1. [Q8WXB1-2]
RefSeqiNP_001120867.1. NM_001127395.3. [Q8WXB1-1]
NP_001294950.1. NM_001308021.2.
NP_001317059.1. NM_001330130.1.
NP_001317060.1. NM_001330131.1.
NP_001317062.1. NM_001330133.1.
NP_001317063.1. NM_001330134.1.
NP_001317064.1. NM_001330135.1.
NP_660323.3. NM_145280.6. [Q8WXB1-1]
XP_005246396.1. XM_005246339.3. [Q8WXB1-1]
XP_005246397.1. XM_005246340.3. [Q8WXB1-1]
UniGeneiHs.664764.
Hs.734938.

Genome annotation databases

EnsembliENST00000406927; ENSP00000385481; ENSG00000144401. [Q8WXB1-1]
ENST00000411432; ENSP00000415115; ENSG00000144401. [Q8WXB1-1]
ENST00000425132; ENSP00000400730; ENSG00000144401. [Q8WXB1-2]
ENST00000426075; ENSP00000403317; ENSG00000144401. [Q8WXB1-1]
ENST00000442521; ENSP00000392062; ENSG00000144401. [Q8WXB1-1]
ENST00000448007; ENSP00000407622; ENSG00000144401. [Q8WXB1-1]
ENST00000458426; ENSP00000389684; ENSG00000144401. [Q8WXB1-2]
GeneIDi151194.
KEGGihsa:151194.
UCSCiuc002vce.4. human. [Q8WXB1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF455817 mRNA. Translation: AAL66295.1.
AK093812 mRNA. Translation: BAC04229.1.
AC079767 Genomic DNA. Translation: AAX93175.1.
BC009462 mRNA. Translation: AAH09462.1.
BC033720 mRNA. Translation: AAH33720.1.
CCDSiCCDS2376.1. [Q8WXB1-1]
CCDS82562.1. [Q8WXB1-2]
RefSeqiNP_001120867.1. NM_001127395.3. [Q8WXB1-1]
NP_001294950.1. NM_001308021.2.
NP_001317059.1. NM_001330130.1.
NP_001317060.1. NM_001330131.1.
NP_001317062.1. NM_001330133.1.
NP_001317063.1. NM_001330134.1.
NP_001317064.1. NM_001330135.1.
NP_660323.3. NM_145280.6. [Q8WXB1-1]
XP_005246396.1. XM_005246339.3. [Q8WXB1-1]
XP_005246397.1. XM_005246340.3. [Q8WXB1-1]
UniGeneiHs.664764.
Hs.734938.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LECX-ray2.28A/B8-218[»]
ProteinModelPortaliQ8WXB1.
SMRiQ8WXB1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127352. 20 interactors.
IntActiQ8WXB1. 6 interactors.
STRINGi9606.ENSP00000385481.

Chemistry databases

ChEMBLiCHEMBL3588741.

PTM databases

iPTMnetiQ8WXB1.
PhosphoSitePlusiQ8WXB1.

Polymorphism and mutation databases

BioMutaiMETTL21A.
DMDMi150382834.

Proteomic databases

EPDiQ8WXB1.
MaxQBiQ8WXB1.
PaxDbiQ8WXB1.
PeptideAtlasiQ8WXB1.
PRIDEiQ8WXB1.

Protocols and materials databases

DNASUi151194.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000406927; ENSP00000385481; ENSG00000144401. [Q8WXB1-1]
ENST00000411432; ENSP00000415115; ENSG00000144401. [Q8WXB1-1]
ENST00000425132; ENSP00000400730; ENSG00000144401. [Q8WXB1-2]
ENST00000426075; ENSP00000403317; ENSG00000144401. [Q8WXB1-1]
ENST00000442521; ENSP00000392062; ENSG00000144401. [Q8WXB1-1]
ENST00000448007; ENSP00000407622; ENSG00000144401. [Q8WXB1-1]
ENST00000458426; ENSP00000389684; ENSG00000144401. [Q8WXB1-2]
GeneIDi151194.
KEGGihsa:151194.
UCSCiuc002vce.4. human. [Q8WXB1-1]

Organism-specific databases

CTDi151194.
DisGeNETi151194.
GeneCardsiMETTL21A.
HGNCiHGNC:30476. METTL21A.
HPAiHPA034712.
MIMi615257. gene.
neXtProtiNX_Q8WXB1.
OpenTargetsiENSG00000144401.
PharmGKBiPA145008433.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2793. Eukaryota.
ENOG4111IJ0. LUCA.
GeneTreeiENSGT00550000074572.
HOVERGENiHBG059353.
InParanoidiQ8WXB1.
PhylomeDBiQ8WXB1.
TreeFamiTF313206.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000144401-MONOMER.

Miscellaneous databases

ChiTaRSiMETTL21A. human.
GenomeRNAii151194.
PROiQ8WXB1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000144401.
CleanExiHS_FAM119A.
ExpressionAtlasiQ8WXB1. baseline and differential.
GenevisibleiQ8WXB1. HS.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR019410. Methyltransf_16.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF10294. Methyltransf_16. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMT21A_HUMAN
AccessioniPrimary (citable) accession number: Q8WXB1
Secondary accession number(s): Q53RV0, Q8N1Z9, Q96GH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: November 30, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families
  7. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.