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Protein

Splicing regulatory glutamine/lysine-rich protein 1

Gene

SREK1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the regulation of alternative splicing by modulating the activity of other splice facors. Inhibits the splicing activity of SFRS1, SFRS2 and SFRS6. Augments the splicing activity of SFRS3 (By similarity).By similarity

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing regulatory glutamine/lysine-rich protein 1
Alternative name(s):
Serine/arginine-rich-splicing regulatory protein 86
Short name:
SRrp86
Splicing factor, arginine/serine-rich 12
Splicing regulatory protein 508
Short name:
SRrp508
Gene namesi
Name:SREK1
Synonyms:SFRS12, SRRP86
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:17882. SREK1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38255.

Polymorphism and mutation databases

BioMutaiSREK1.
DMDMi37537968.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 508508Splicing regulatory glutamine/lysine-rich protein 1PRO_0000081940Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei184 – 1841Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8WXA9.
PaxDbiQ8WXA9.
PeptideAtlasiQ8WXA9.
PRIDEiQ8WXA9.

PTM databases

PhosphoSiteiQ8WXA9.

Expressioni

Gene expression databases

BgeeiQ8WXA9.
CleanExiHS_SFRS12.
ExpressionAtlasiQ8WXA9. baseline and differential.
GenevisibleiQ8WXA9. HS.

Organism-specific databases

HPAiHPA037674.
HPA059332.

Interactioni

Subunit structurei

Homodimer. Binds SFRS1, SFRS2, SFRS3 and SFRS6. Interacts with the spliceosome (By similarity). Interacts with SREK1IP1.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SRSF11Q055193EBI-1044237,EBI-1051785

Protein-protein interaction databases

BioGridi126754. 70 interactions.
IntActiQ8WXA9. 8 interactions.
MINTiMINT-4535913.
STRINGi9606.ENSP00000334538.

Structurei

3D structure databases

ProteinModelPortaliQ8WXA9.
SMRiQ8WXA9. Positions 64-142.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – 14277RRMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi190 – 474285Arg/Glu/Lys/Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG277302.
GeneTreeiENSGT00730000110872.
HOVERGENiHBG079174.
InParanoidiQ8WXA9.
KOiK13165.
OMAiCQPNEES.
OrthoDBiEOG7ZD1VT.
PhylomeDBiQ8WXA9.
TreeFamiTF106266.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WXA9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTSLMPGAGL LPIPTPNPLT TLGVSLSSLG AIPAAALDPN IATLGEIPQP
60 70 80 90 100
PLMGNVDPSK IDEIRRTVYV GNLNSQTTTA DQLLEFFKQV GEVKFVRMAG
110 120 130 140 150
DETQPTRFAF VEFADQNSVP RALAFNGVMF GDRPLKINHS NNAIVKPPEM
160 170 180 190 200
TPQAAAKELE EVMKRVREAQ SFISAAIEPE SGKSNERKGG RSRSHTRSKS
210 220 230 240 250
RSSSKSHSRR KRSQSKHRSR SHNRSRSRQK DRRRSKSPHK KRSKSRERRK
260 270 280 290 300
SRSRSHSRDK RKDTREKIKE KERVKEKDRE KEREREKERE KEKERGKNKD
310 320 330 340 350
RDKEREKDRE KDKEKDRERE REKEHEKDRD KEKEKEQDKE KEREKDRSKE
360 370 380 390 400
IDEKRKKDKK SRTPPRSYNA SRRSRSSSRE RRRRRSRSSS RSPRTSKTIK
410 420 430 440 450
RKSSRSPSPR SRNKKDKKRE KERDHISERR ERERSTSMRK SSNDRDGKEK
460 470 480 490 500
LEKNSTSLKE KEHNKEPDSS VSKEVDDKDA PRTEENKIQH NGNCQLNEEN

LSTKTEAV
Length:508
Mass (Da):59,380
Last modified:March 1, 2002 - v1
Checksum:i878DC088B642EC3F
GO
Isoform 2 (identifier: Q8WXA9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MNSGGGFGLG...LSLLAPAPTM

Note: No experimental confirmation available.
Show »
Length:624
Mass (Da):71,649
Checksum:i86DBE20EDCF3EC6F
GO

Sequence cautioni

The sequence AAH47322.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MNSGGGFGLGLGFGLTPTSV IQVTNLSSAVTSEQMRTLFS FLGEIEELRLYPPDNAPLAF SSKVCYVKFRDPSSVGVAQH LTNTVFIDRALIVVPCAEGK IPEESKALSLLAPAPTM in isoform 2. 1 PublicationVSP_008399

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF459094 mRNA. Translation: AAL67778.1.
BC047322 mRNA. Translation: AAH47322.1. Sequence problems.
BC067770 mRNA. Translation: AAH67770.1.
BC112343 mRNA. Translation: AAI12344.1.
CCDSiCCDS3991.1. [Q8WXA9-1]
CCDS43323.1. [Q8WXA9-2]
RefSeqiNP_001070667.1. NM_001077199.2. [Q8WXA9-2]
NP_001257421.1. NM_001270492.1.
NP_631907.1. NM_139168.3. [Q8WXA9-1]
XP_005248496.1. XM_005248439.1. [Q8WXA9-1]
XP_011541473.1. XM_011543171.1. [Q8WXA9-1]
UniGeneiHs.49367.

Genome annotation databases

EnsembliENST00000334121; ENSP00000334538; ENSG00000153914. [Q8WXA9-2]
ENST00000380918; ENSP00000370305; ENSG00000153914.
GeneIDi140890.
KEGGihsa:140890.
UCSCiuc003jun.4. human. [Q8WXA9-2]
uc003juo.4. human. [Q8WXA9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF459094 mRNA. Translation: AAL67778.1.
BC047322 mRNA. Translation: AAH47322.1. Sequence problems.
BC067770 mRNA. Translation: AAH67770.1.
BC112343 mRNA. Translation: AAI12344.1.
CCDSiCCDS3991.1. [Q8WXA9-1]
CCDS43323.1. [Q8WXA9-2]
RefSeqiNP_001070667.1. NM_001077199.2. [Q8WXA9-2]
NP_001257421.1. NM_001270492.1.
NP_631907.1. NM_139168.3. [Q8WXA9-1]
XP_005248496.1. XM_005248439.1. [Q8WXA9-1]
XP_011541473.1. XM_011543171.1. [Q8WXA9-1]
UniGeneiHs.49367.

3D structure databases

ProteinModelPortaliQ8WXA9.
SMRiQ8WXA9. Positions 64-142.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126754. 70 interactions.
IntActiQ8WXA9. 8 interactions.
MINTiMINT-4535913.
STRINGi9606.ENSP00000334538.

PTM databases

PhosphoSiteiQ8WXA9.

Polymorphism and mutation databases

BioMutaiSREK1.
DMDMi37537968.

Proteomic databases

MaxQBiQ8WXA9.
PaxDbiQ8WXA9.
PeptideAtlasiQ8WXA9.
PRIDEiQ8WXA9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000334121; ENSP00000334538; ENSG00000153914. [Q8WXA9-2]
ENST00000380918; ENSP00000370305; ENSG00000153914.
GeneIDi140890.
KEGGihsa:140890.
UCSCiuc003jun.4. human. [Q8WXA9-2]
uc003juo.4. human. [Q8WXA9-1]

Organism-specific databases

CTDi140890.
GeneCardsiGC05P065435.
HGNCiHGNC:17882. SREK1.
HPAiHPA037674.
HPA059332.
MIMi609268. gene.
neXtProtiNX_Q8WXA9.
PharmGKBiPA38255.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG277302.
GeneTreeiENSGT00730000110872.
HOVERGENiHBG079174.
InParanoidiQ8WXA9.
KOiK13165.
OMAiCQPNEES.
OrthoDBiEOG7ZD1VT.
PhylomeDBiQ8WXA9.
TreeFamiTF106266.

Miscellaneous databases

ChiTaRSiSREK1. human.
GeneWikiiSFRS12.
GenomeRNAii140890.
NextBioi84548.
PROiQ8WXA9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8WXA9.
CleanExiHS_SFRS12.
ExpressionAtlasiQ8WXA9. baseline and differential.
GenevisibleiQ8WXA9. HS.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, characterization, chromosomal assignment, genomic organization and verification of SFRS12(SRrp508), a novel member of human SR protein superfamily and a human homolog of rat SRrp86."
    Zhang D.L., Sun X.J., Ling L.J., Chen R.S., Ma D.L.
    Yi Chuan Xue Bao 29:377-383(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Pancreas.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Mammary gland and Testis.
  3. "Large-scale proteomic analysis of the human spliceosome."
    Rappsilber J., Ryder U., Lamond A.I., Mann M.
    Genome Res. 12:1231-1245(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, INTERACTION WITH THE SPLICEOSOME, IDENTIFICATION BY MASS SPECTROMETRY.
  4. "The splicing regulatory protein p18SRP is down-regulated in Alzheimer's disease brain."
    Heese K., Fujita M., Akatsu H., Yamamoto T., Kosaka K., Nagai Y., Sawada T.
    J. Mol. Neurosci. 24:269-276(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SREK1IP1.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-184, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiSREK1_HUMAN
AccessioniPrimary (citable) accession number: Q8WXA9
Secondary accession number(s): A4FTW3, Q2M1J0, Q86X37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: March 1, 2002
Last modified: July 22, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.