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Protein

Negative elongation factor B

Gene

NELFB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding.4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor, RNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-112382. Formation of RNA Pol II elongation complex.
R-HSA-112387. Elongation arrest and recovery.
R-HSA-113418. Formation of the Early Elongation Complex.
R-HSA-167152. Formation of HIV elongation complex in the absence of HIV Tat.
R-HSA-167158. Formation of the HIV-1 Early Elongation Complex.
R-HSA-167200. Formation of HIV-1 elongation complex containing HIV-1 Tat.
R-HSA-167238. Pausing and recovery of Tat-mediated HIV elongation.
R-HSA-167242. Abortive elongation of HIV-1 transcript in the absence of Tat.
R-HSA-167243. Tat-mediated HIV elongation arrest and recovery.
R-HSA-167246. Tat-mediated elongation of the HIV-1 transcript.
R-HSA-167287. HIV elongation arrest and recovery.
R-HSA-167290. Pausing and recovery of HIV elongation.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-HSA-75955. RNA Polymerase II Transcription Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
Negative elongation factor B
Short name:
NELF-B
Alternative name(s):
Cofactor of BRCA1
Gene namesi
Name:NELFB
Synonyms:COBRA1, KIAA1182
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:24324. NELFB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: LIFEdb
  • NELF complex Source: UniProtKB
  • nucleoplasm Source: HPA

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi25920.
PharmGKBiPA162382703.

Polymorphism and mutation databases

BioMutaiNELFB.
DMDMi38372378.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191291 – 580Negative elongation factor BAdd BLAST580

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei519N6-acetyllysineCombined sources1
Modified residuei557PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8WX92.
MaxQBiQ8WX92.
PaxDbiQ8WX92.
PeptideAtlasiQ8WX92.
PRIDEiQ8WX92.

PTM databases

iPTMnetiQ8WX92.
PhosphoSitePlusiQ8WX92.
SwissPalmiQ8WX92.

Expressioni

Tissue specificityi

Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.1 Publication

Gene expression databases

BgeeiENSG00000188986.
CleanExiHS_COBRA1.
ExpressionAtlasiQ8WX92. baseline and differential.
GenevisibleiQ8WX92. HS.

Organism-specific databases

HPAiHPA020259.

Interactioni

Subunit structurei

The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE; the N-terminus of NELFB binds to the NELFA:NELFCD subcomplex. Interacts with the first BRCT repeat of BRCA1. Interacts with KIAA1191.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi117421. 52 interactors.
DIPiDIP-32670N.
IntActiQ8WX92. 27 interactors.
MINTiMINT-1033357.
STRINGi9606.ENSP00000339495.

Structurei

3D structure databases

ProteinModelPortaliQ8WX92.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NELF-B family.Curated

Phylogenomic databases

eggNOGiENOG410IEBF. Eukaryota.
ENOG410YK9P. LUCA.
HOGENOMiHOG000231489.
HOVERGENiHBG052596.
InParanoidiQ8WX92.
KOiK15180.
OrthoDBiEOG091G04WH.
PhylomeDBiQ8WX92.
TreeFamiTF324620.

Family and domain databases

InterProiView protein in InterPro
IPR010405. COBRA1.
PANTHERiPTHR13503. PTHR13503. 1 hit.
PfamiView protein in Pfam
PF06209. COBRA1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8WX92-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFAGLQDLGV ANGEDLKETL TNCTEPLKAI EQFQTENGVL LPSLQSALPF
60 70 80 90 100
LDLHGTPRLE FHQSVFDELR DKLLERVSAI ASEGKAEERY KKLEDLLEKS
110 120 130 140 150
FSLVKMPSLQ PVVMCVMKHL PKVPEKKLKL VMADKELYRA CAVEVKRQIW
160 170 180 190 200
QDNQALFGDE VSPLLKQYIL EKESALFSTE LSVLHNFFSP SPKTRRQGEV
210 220 230 240 250
VQRLTRMVGK NVKLYDMVLQ FLRTLFLRTR NVHYCTLRAE LLMSLHDLDV
260 270 280 290 300
GEICTVDPCH KFTWCLDACI RERFVDSKRA RELQGFLDGV KKGQEQVLGD
310 320 330 340 350
LSMILCDPFA INTLALSTVR HLQELVGQET LPRDSPDLLL LLRLLALGQG
360 370 380 390 400
AWDMIDSQVF KEPKMEVELI TRFLPMLMSF LVDDYTFNVD QKLPAEEKAP
410 420 430 440 450
VSYPNTLPES FTKFLQEQRM ACEVGLYYVL HITKQRNKNA LLRLLPGLVE
460 470 480 490 500
TFGDLAFGDI FLHLLTGNLA LLADEFALED FCSSLFDGFF LTASPRKENV
510 520 530 540 550
HRHALRLLIH LHPRVAPSKL EALQKALEPT GQSGEAVKEL YSQLGEKLEQ
560 570 580
LDHRKPSPAQ AAETPALELP LPSVPAPAPL
Length:580
Mass (Da):65,697
Last modified:March 1, 2002 - v1
Checksum:iC0A4F7F2BEE52CBD
GO

Sequence cautioni

The sequence AAH11892 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB14157 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAB43381 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti156L → F in BAB14157 (PubMed:14702039).Curated1
Sequence conflicti182S → A (PubMed:11230166).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF464935 mRNA. Translation: AAL69965.1.
AK022651 mRNA. Translation: BAB14157.1. Different initiation.
AK091056 mRNA. Translation: BAG52272.1.
BX255925 Genomic DNA. No translation available.
BC011892 mRNA. Translation: AAH11892.1. Different initiation.
AL050280 mRNA. Translation: CAB43381.3. Different initiation.
AB033008 mRNA. Translation: BAA86496.1.
PIRiT08747.
RefSeqiNP_056271.3. NM_015456.4.
UniGeneiHs.521809.

Genome annotation databases

EnsembliENST00000634710; ENSP00000489248; ENSG00000188986.
GeneIDi25920.
KEGGihsa:25920.
UCSCiuc004cmm.5. human.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNELFB_HUMAN
AccessioniPrimary (citable) accession number: Q8WX92
Secondary accession number(s): A2BFA3
, Q96EW5, Q9H9R4, Q9ULN8, Q9Y3W0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: March 1, 2002
Last modified: June 7, 2017
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families