Q8WWZ8 (OIT3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oncoprotein-induced transcript 3 protein

Alternative name(s):
Liver-specific zona pellucida domain-containing protein

Gene names

Name:	OIT3
Synonyms:	LZP
ORF Names:	UNQ826/PRO1753

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in hepatocellular function and development. Ref.1
Subcellular location	Nucleus envelope. Note: Can be secreted into blood. Ref.1
Tissue specificity	Liver-specific. Expressed only in the hepatocytes. Down-regulated in hepatocellular carcinoma (HCC) and HCC cell lines. Ref.1
Sequence similarities	Contains 1 EGF-like domain. Contains 1 ZP domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	EGF-like domain Signal
Ligand	Calcium
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	nuclear envelope Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8WWZ8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8WWZ8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     318-321: VVND → LCFR
     322-545: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 545	523	Oncoprotein-induced transcript 3 protein		PRO_0000298931
Regions
	Domain	182 – 222	41	EGF-like; calcium-binding Potential
	Domain	261 – 516	256	ZP
Amino acid modifications
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	116	1	N-linked (GlcNAc...) Potential
	Glycosylation	291	1	N-linked (GlcNAc...) Ref.5
	Glycosylation	299	1	N-linked (GlcNAc...) Potential
	Disulfide bond	186 ↔ 197		By similarity
	Disulfide bond	193 ↔ 206		By similarity
	Disulfide bond	208 ↔ 221		By similarity
Natural variations
	Alternative sequence	318 – 321	4	VVND → LCFR in isoform 2.		VSP_027482
	Alternative sequence	322 – 545	224	Missing in isoform 2.		VSP_027483
	Natural variant	237	1	S → P. Corresponds to variant rs35089256 [ dbSNP | Ensembl ].		VAR_034742

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 2. Checksum: E4BDE126213484B0	FASTA	545	60,022
	10 20 30 40 50 60 MPPFLLLTCL FITGTSVSPV ALDPCSAYIS LNEPWRNTDH QLDESQGPPL CDNHVNGEWY 70 80 90 100 110 120 HFTGMAGDAM PTFCIPENHC GTHAPVWLNG SHPLEGDGIV QRQACASFNG NCCLWNTTVE 130 140 150 160 170 180 VKACPGGYYV YRLTKPSVCF HVYCGHFYDI CDEDCHGSCS DTSECTCAPG TVLGPDRQTC 190 200 210 220 230 240 FDENECEQNN GGCSEICVNL KNSYRCECGV GRVLRSDGKT CEDVEGCHNN NGGCSHSCLG 250 260 270 280 290 300 SEKGYQCECP RGLVLSEDNH TCQVPVLCKS NAIEVNIPRE LVGGLELFLT NTSCRGVSNG 310 320 330 340 350 360 THVNILFSLK TCGTVVDVVN DKIVASNLVT GLPKQTPGSS GDFIIRTSKL LIPVTCEFPR 370 380 390 400 410 420 LYTISEGYVP NLRNSPLEIM SRNHGIFPFT LEIFKDNEFE EPYREALPTL KLRDSLYFGI 430 440 450 460 470 480 EPVVHVSGLE SLVESCFATP TSKIDEVLKY YLIRDGCVSD DSVKQYTSRD HLAKHFQVPV 490 500 510 520 530 540 FKFVGKDHKE VFLHCRVLVC GVLDERSRCA QGCHRRMRRG AGGEDSAGLQ GQTLTGGPIR IDWED
	Isoform 2 [UniParc]. Checksum: 9AF8C61098ED67A4	FASTA	321	34,911
	10 20 30 40 50 60 MPPFLLLTCL FITGTSVSPV ALDPCSAYIS LNEPWRNTDH QLDESQGPPL CDNHVNGEWY 70 80 90 100 110 120 HFTGMAGDAM PTFCIPENHC GTHAPVWLNG SHPLEGDGIV QRQACASFNG NCCLWNTTVE 130 140 150 160 170 180 VKACPGGYYV YRLTKPSVCF HVYCGHFYDI CDEDCHGSCS DTSECTCAPG TVLGPDRQTC 190 200 210 220 230 240 FDENECEQNN GGCSEICVNL KNSYRCECGV GRVLRSDGKT CEDVEGCHNN NGGCSHSCLG 250 260 270 280 290 300 SEKGYQCECP RGLVLSEDNH TCQVPVLCKS NAIEVNIPRE LVGGLELFLT NTSCRGVSNG 310 320 THVNILFSLK TCGTVVDLCF R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel liver-specific zona pellucida domain containing protein that is expressed rarely in hepatocellular carcinoma." Xu Z.-G., Du J.-J., Zhang X., Cheng Z.-H., Ma Z.-Z., Xiao H.-S., Yu L., Wang Z.-Q., Li Y.-Y., Huo K.-K., Han Z.-G. Hepatology 38:735-744(2003) [PubMed: 12939600] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Liver.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Tongue.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lung.
[5]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-291, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY013707 mRNA. Translation: AAG40096.2. AY358339 mRNA. Translation: AAQ88705.1. AK096435 mRNA. Translation: BAC04788.1. BC126440 mRNA. Translation: AAI26441.1.
IPI	IPI00328215. IPI00855752.
RefSeq	NP_689848.1. NM_152635.1.
UniGene	Hs.8366.
3D structure databases
HSSP	HSSP built from PDB template 1HZ8 based on UniProtKB P01130.
ProteinModelPortal	Q8WWZ8.
SMR	Q8WWZ8. Positions 118-302.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8WWZ8.
PTM databases
PhosphoSite	Q8WWZ8.
Polymorphism databases
DMDM	74730985.
Proteomic databases
PRIDE	Q8WWZ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000334011; ENSP00000333900; ENSG00000138315.
GeneID	170392.
KEGG	hsa:170392.
UCSC	uc001jte.1. human.
Organism-specific databases
CTD	170392.
GeneCards	GC10P074654.
H-InvDB	HIX0008916.
HGNC	HGNC:29953. OIT3.
MIM	609330. gene.
neXtProt	NX_Q8WWZ8.
PharmGKB	PA142671231.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG10091.
GeneTree	ENSGT00530000063244.
HOGENOM	HBG280181.
HOVERGEN	HBG097756.
InParanoid	Q8WWZ8.
OMA	NHGIFPF.
OrthoDB	EOG4CRKZN.
PhylomeDB	Q8WWZ8.
Gene expression databases
ArrayExpress	Q8WWZ8.
Bgee	Q8WWZ8.
CleanEx	HS_OIT3.
Genevestigator	Q8WWZ8.
Family and domain databases
InterPro	IPR006210. EGF-like. IPR001881. EGF-like_Ca-bd. IPR000152. EGF-type_Asp/Asn_hydroxyl_site. IPR018097. EGF_Ca-bd_CS. IPR017976. Endoglin/CD105_subgr. IPR001507. Zona_pellucida_Endoglin/CD105. [Graphical view]
Pfam	PF00100. Zona_pellucida. 1 hit. [Graphical view]
PRINTS	PR00023. ZPELLUCIDA.
SMART	SM00181. EGF. 1 hit. SM00179. EGF_CA. 1 hit. SM00241. ZP. 1 hit. [Graphical view]
PROSITE	PS00010. ASX_HYDROXYL. 1 hit. PS00022. EGF_1. False negative. PS01186. EGF_2. False negative. PS50026. EGF_3. False negative. PS01187. EGF_CA. 1 hit. PS00682. ZP_1. False negative. PS51034. ZP_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	88911.
SOURCE	Search...

Entry information

Entry name

OIT3_HUMAN

Accession

Primary (citable) accession number: Q8WWZ8
Secondary accession number(s): A0AVP3, Q8N1M8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.