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Q8WWT9 (S13A3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 13 member 3
Alternative name(s):
Na(+)/dicarboxylate cotransporter 3
Short name=NaDC-3
Short name=hNaDC3
Sodium-dependent high-affinity dicarboxylate transporter 2
Gene names
Name:SLC13A3
Synonyms:NADC3, SDCT2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

High-affinity sodium-dicarboxylate cotransporter that accepts a range of substrates with 4-5 carbon atoms. The stoichiometry is probably 3 Na+ for 1 divalent succinate.

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.8.

Tissue specificity

Expression is highest in kidney. Detected in placenta, brain, liver and pancreas.

Sequence similarities

Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily. [View classification]

Sequence caution

The sequence AAF73251.1 differs from that shown. Reason: Frameshift at positions 188 and 199.

The sequence CAC09447.2 differs from that shown. Reason: erroneous CDS prediction.

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8WWT9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8WWT9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     181-297: AAVRRNGLHT...LFLLAGWLWI → GIEPNTFLSE...RVLSFLPGGM
     298-602: Missing.
Note: Gene prediction based on EST data.
Isoform 3 (identifier: Q8WWT9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: Missing.
     340-374: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q8WWT9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     182-292: AVRRNGLHTV...FPLMLLFLLA → KTTLGRQRFH...LTSSCLASSR
     375-388: FLSDAVTGVAIVTI → CKMGIISISTIQKM
     389-602: Missing.
Note: No experimental confirmation available. Gene prediction based on EST data.
Isoform 5 (identifier: Q8WWT9-5)

The sequence of this isoform differs from the canonical sequence as follows:
     182-292: AVRRNGLHTV...FPLMLLFLLA → KTTLGRQRFH...LTSSCLASSR
Note: No experimental confirmation available.
Isoform 6 (identifier: Q8WWT9-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: Missing.
Note: No experimental confirmation available. Gene prediction based on EST data.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 602602Solute carrier family 13 member 3
PRO_0000172492

Regions

Topological domain1 – 1616Cytoplasmic Potential
Transmembrane17 – 3721Helical; Potential
Topological domain38 – 5518Extracellular Potential
Transmembrane56 – 7621Helical; Potential
Topological domain77 – 826Cytoplasmic Potential
Transmembrane83 – 10321Helical; Potential
Topological domain104 – 13734Extracellular Potential
Transmembrane138 – 15821Helical; Potential
Topological domain159 – 22971Cytoplasmic Potential
Transmembrane230 – 25021Helical; Potential
Topological domain251 – 27828Extracellular Potential
Transmembrane279 – 29921Helical; Potential
Topological domain300 – 33637Cytoplasmic Potential
Transmembrane337 – 35721Helical; Potential
Topological domain358 – 37215Extracellular Potential
Transmembrane373 – 39321Helical; Potential
Topological domain394 – 42229Cytoplasmic Potential
Intramembrane423 – 44321Helical; Potential
Topological domain444 – 46118Cytoplasmic Potential
Transmembrane462 – 48221Helical; Potential
Topological domain483 – 50523Extracellular Potential
Transmembrane506 – 52621Helical; Potential
Topological domain527 – 54620Cytoplasmic Potential
Transmembrane547 – 56721Helical; Potential
Topological domain568 – 60235Extracellular Potential

Amino acid modifications

Glycosylation5861N-linked (GlcNAc...) Potential
Glycosylation5961N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 4747Missing in isoform 3 and isoform 6.
VSP_015291
Alternative sequence181 – 297117AAVRR…GWLWI → GIEPNTFLSEERLKLQAPLV IRLGQITESGQWNMSGNDVC NFRVLSFLPGGM in isoform 2.
VSP_006123
Alternative sequence182 – 292111AVRRN…LFLLA → KTTLGRQRFHWICRLTPGRR MNIVGTSGRASSSPSPTQPV LGAQPHSRAQPLTSSCLASS R in isoform 4 and isoform 5.
VSP_015292
Alternative sequence298 – 602305Missing in isoform 2.
VSP_006124
Alternative sequence340 – 37435Missing in isoform 3.
VSP_015293
Alternative sequence375 – 38814FLSDA…AIVTI → CKMGIISISTIQKM in isoform 4.
VSP_015294
Alternative sequence389 – 602214Missing in isoform 4.
VSP_015295

Experimental info

Sequence conflict941L → P in BAC04834. Ref.3
Sequence conflict3641I → V in AAH35966. Ref.6
Sequence conflict5721Q → P in BAB71262. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 611A50994BA37687

FASTA60266,841
        10         20         30         40         50         60 
MAALAAAAKK VWSARRLLVL LFTPLALLPV VFALPPKEGR CLFVILLMAV YWCTEALPLS 

        70         80         90        100        110        120 
VTALLPIVLF PFMGILPSNK VCPQYFLDTN FLFLSGLIMA SAIEEWNLHR RIALKILMLV 

       130        140        150        160        170        180 
GVQPARLILG MMVTTSFLSM WLSNTASTAM MLPIANAILK SLFGQKEVRK DPSQESEENT 

       190        200        210        220        230        240 
AAVRRNGLHT VPTEMQFLAS TEAKDHPGET EVPLDLPADS RKEDEYRRNI WKGFLISIPY 

       250        260        270        280        290        300 
SASIGGTATL TGTAPNLILL GQLKSFFPQC DVVNFGSWFI FAFPLMLLFL LAGWLWISFL 

       310        320        330        340        350        360 
YGGLSFRGWR KNKSEIRTNA EDRARAVIRE EYQNLGPIKF AEQAVFILFC MFAILLFTRD 

       370        380        390        400        410        420 
PKFIPGWASL FNPGFLSDAV TGVAIVTILF FFPSQRPSLK WWFDFKAPNT ETEPLLTWKK 

       430        440        450        460        470        480 
AQETVPWNII LLLGGGFAMA KGCEESGLSV WIGGQLHPLE NVPPALAVLL ITVVIAFFTE 

       490        500        510        520        530        540 
FASNTATIII FLPVLAELAI RLRVHPLYLM IPGTVGCSFA FMLPVSTPPN SIAFASGHLL 

       550        560        570        580        590        600 
VKDMVRTGLL MNLMGVLLLS LAMNTWAQTI FQLGTFPDWA DMYSVNVTAL PPTLANDTFR 


TL 

« Hide

Isoform 2 [UniParc].

Checksum: 7EEDA215B050ABEB
Show »

FASTA23225,684
Isoform 3 [UniParc].

Checksum: D9CB6E6BE1A5C936
Show »

FASTA52057,766
Isoform 4 [UniParc].

Checksum: 022516BECC8D3961
Show »

FASTA33837,669
Isoform 5 [UniParc].

Checksum: EF5734E90BBE2167
Show »

FASTA55261,099
Isoform 6 [UniParc].

Checksum: E433B6A826BA4D77
Show »

FASTA55561,757

References

« Hide 'large scale' references
[1]"Structure, function, and genomic organization of human Na(+)-dependent high-affinity dicarboxylate transporter."
Wang H., Fei Y.-J., Kekuda R., Yang-Feng T.L., Devoe L.D., Leibach F.H., Prasad P.D., Ganapathy V.
Am. J. Physiol. 278:C1019-C1030(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Placenta.
[2]"Cloning and characterization of energy metabolism-related sodium-dependent high-affinity dicarboxylate transporter gene from human kidney."
Bai X.-Y., Chen X.-M., Qiu Q.
Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Kidney.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3 AND 5).
Tissue: Brain, Hippocampus and Placenta.
[4]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Kidney.
[7]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 433-602 (ISOFORM 1).
Tissue: Brain.
[8]"Membrane topology structure of human high-affinity, sodium-dependent dicarboxylate transporter."
Bai X.Y., Chen X., Sun A.Q., Feng Z., Hou K., Fu B.
FASEB J. 21:2409-2417(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, MEMBRANE TOPOLOGY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF154121 mRNA. Translation: AAF73251.1. Frameshift.
AY072810 mRNA. Translation: AAL66762.1.
AK056713 mRNA. Translation: BAB71262.1.
AK096658 mRNA. Translation: BAC04834.1.
AK295748 mRNA. Translation: BAG58580.1.
AL133520 Genomic DNA. No translation available.
AL034424 Genomic DNA. No translation available.
CH471077 Genomic DNA. Translation: EAW75725.1.
CH471077 Genomic DNA. Translation: EAW75726.1.
BC035966 mRNA. Translation: AAH35966.1.
AL442082 mRNA. Translation: CAC09447.2. Sequence problems.
RefSeqNP_001011554.1. NM_001011554.2.
NP_001180268.1. NM_001193339.1.
NP_001180269.1. NM_001193340.1.
NP_073740.2. NM_022829.5.
UniGeneHs.655498.
Hs.670365.

3D structure databases

ProteinModelPortalQ8WWT9.
SMRQ8WWT9. Positions 15-568.
ModBaseSearch...
MobiDBSearch...

Chemistry

DrugBankDB00139. Succinic acid.

Protein family/group databases

TCDB2.A.47.1.15. the divalent anion:na(+) symporter (dass) family.

PTM databases

PhosphoSiteQ8WWT9.

Polymorphism databases

DMDM23396845.

Proteomic databases

PaxDbQ8WWT9.
PRIDEQ8WWT9.

Protocols and materials databases

DNASU64849.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000279027; ENSP00000279027; ENSG00000158296. [Q8WWT9-1]
ENST00000290317; ENSP00000290317; ENSG00000158296.
ENST00000339636; ENSP00000344912; ENSG00000158296. [Q8WWT9-2]
ENST00000372121; ENSP00000361193; ENSG00000158296. [Q8WWT9-4]
ENST00000396360; ENSP00000379648; ENSG00000158296. [Q8WWT9-3]
ENST00000413164; ENSP00000415852; ENSG00000158296. [Q8WWT9-5]
ENST00000472148; ENSP00000420177; ENSG00000158296. [Q8WWT9-3]
ENST00000495082; ENSP00000419621; ENSG00000158296.
GeneID64849.
KEGGhsa:64849.
UCSCuc002xsf.2. human. [Q8WWT9-1]
uc010gho.2. human. [Q8WWT9-3]
uc010zxw.2. human. [Q8WWT9-5]

Organism-specific databases

CTD64849.
GeneCardsGC20M045186.
HGNCHGNC:14430. SLC13A3.
HPAHPA014353.
HPA014736.
MIM606411. gene.
neXtProtNX_Q8WWT9.
PharmGKBPA37881.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0471.
HOVERGENHBG055339.
InParanoidQ8WWT9.
KOK14445.
OMARTNFSIC.
OrthoDBEOG7G1V5S.
PhylomeDBQ8WWT9.
TreeFamTF312913.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.
REACT_20633. Bile salt and organic anion SLC transporters.

Gene expression databases

ArrayExpressQ8WWT9.
BgeeQ8WWT9.
GenevestigatorQ8WWT9.

Family and domain databases

InterProIPR001898. Na/sul_symport.
[Graphical view]
PfamPF00939. Na_sulph_symp. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSLC13A3.
GenomeRNAi64849.
NextBio66966.
PROQ8WWT9.
SOURCESearch...

Entry information

Entry nameS13A3_HUMAN
AccessionPrimary (citable) accession number: Q8WWT9
Secondary accession number(s): B4DIR8 expand/collapse secondary AC list , E1P5U4, F6WI18, Q5JYC9, Q5JYD0, Q5JYD1, Q5TCQ2, Q8IVB1, Q8N8K4, Q96MM5, Q9BR25, Q9H1G1, Q9H3W4, Q9NQN5, Q9NS04
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: March 1, 2002
Last modified: April 16, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM