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Q8WWN8

- ARAP3_HUMAN

UniProt

Q8WWN8 - ARAP3_HUMAN

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Protein

Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3

Gene
ARAP3, CENTD3
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Acts on ARF6, RAC1, RHOA and CDC42. Plays a role in the internalization of anthrax toxin.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri504 – 52724C4-typeAdd
BLAST

GO - Molecular functioni

  1. ARF GTPase activator activity Source: UniProtKB
  2. phosphatidylinositol-3,4,5-trisphosphate binding Source: UniProtKB
  3. phosphatidylinositol-3,4-bisphosphate binding Source: UniProtKB
  4. protein binding Source: IntAct
  5. Rho GTPase activator activity Source: UniProtKB
  6. zinc ion binding Source: InterPro

GO - Biological processi

  1. cytoskeleton organization Source: UniProtKB
  2. negative regulation of cell migration Source: UniProtKB
  3. negative regulation of Rac protein signal transduction Source: Ensembl
  4. negative regulation of Rho protein signal transduction Source: UniProtKB
  5. regulation of ARF GTPase activity Source: InterPro
  6. regulation of cell shape Source: UniProtKB
  7. regulation of small GTPase mediated signal transduction Source: Reactome
  8. small GTPase mediated signal transduction Source: Reactome
  9. vesicle-mediated transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_11051. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3
Alternative name(s):
Centaurin-delta-3
Short name:
Cnt-d3
Gene namesi
Name:ARAP3
Synonyms:CENTD3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:24097. ARAP3.

Subcellular locationi

Cytoplasm By similarity. Cytoplasmcytoskeleton By similarity. Cell membrane; Peripheral membrane protein By similarity. Cell projectionlamellipodium By similarity. Cell projectionruffle By similarity
Note: Cytoplasmic, and associated with F-actin-rich membrane ruffles and lamellipodia By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytoskeleton Source: UniProtKB-SubCell
  3. cytosol Source: Reactome
  4. lamellipodium Source: UniProtKB
  5. plasma membrane Source: UniProtKB-SubCell
  6. ruffle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi307 – 3082RR → AA: Loss of PtdIns(3,4,5)P3 binding. 1 Publication

Organism-specific databases

PharmGKBiPA164715983.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15441544Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3PRO_0000074215Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1403 – 14031Phosphotyrosine By similarity
Modified residuei1408 – 14081Phosphotyrosine By similarity
Modified residuei1444 – 14441Phosphoserine2 Publications
Modified residuei1480 – 14801Phosphoserine1 Publication

Post-translational modificationi

Tyrosine phosphorylated at a low basal level. PDGF treatment stimulates phosphorylation. Tyrosine phosphorylation is increased in cells that are in the process of becoming attached to a substrate and that start spreading and flattening By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8WWN8.
PaxDbiQ8WWN8.
PeptideAtlasiQ8WWN8.
PRIDEiQ8WWN8.

PTM databases

PhosphoSiteiQ8WWN8.

Expressioni

Gene expression databases

ArrayExpressiQ8WWN8.
BgeeiQ8WWN8.
CleanExiHS_ARAP3.
GenevestigatoriQ8WWN8.

Organism-specific databases

HPAiHPA042887.

Interactioni

Subunit structurei

Interacts (via SAM domain) with INPPL1/SHIP2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SH3KBP1Q96B972EBI-4402732,EBI-346595

Protein-protein interaction databases

BioGridi122163. 3 interactions.
IntActiQ8WWN8. 1 interaction.
MINTiMINT-8098880.
STRINGi9606.ENSP00000239440.

Structurei

Secondary structure

1
1544
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 135
Helixi14 – 163
Helixi19 – 213
Helixi22 – 276
Helixi33 – 364
Helixi41 – 477
Helixi52 – 6211
Turni63 – 675

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KG5NMR-A1-80[»]
2LNWNMR-B1404-1412[»]
ProteinModelPortaliQ8WWN8.
SMRiQ8WWN8. Positions 1-80, 293-485, 491-653, 906-1100.

Miscellaneous databases

EvolutionaryTraceiQ8WWN8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 6865SAMAdd
BLAST
Domaini287 – 37993PH 1Add
BLAST
Domaini394 – 48390PH 2Add
BLAST
Domaini480 – 611132Arf-GAPAdd
BLAST
Domaini907 – 1088182Rho-GAPAdd
BLAST
Domaini1117 – 121094Ras-associatingAdd
BLAST
Domaini1223 – 1325103PH 3Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi81 – 13959Pro-richAdd
BLAST
Compositional biasi1462 – 154281Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 Arf-GAP domain.
Contains 3 PH domains.
Contains 1 Rho-GAP domain.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri504 – 52724C4-typeAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5347.
HOGENOMiHOG000246988.
HOVERGENiHBG069114.
InParanoidiQ8WWN8.
KOiK12490.
OMAiTQLLCVE.
PhylomeDBiQ8WWN8.
TreeFamiTF105769.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
1.10.555.10. 1 hit.
2.30.29.30. 3 hits.
InterProiIPR001164. ArfGAP.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000159. Ras-assoc.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
PF00169. PH. 2 hits.
PF00788. RA. 1 hit.
PF00620. RhoGAP. 1 hit.
PF07647. SAM_2. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
SM00233. PH. 5 hits.
SM00324. RhoGAP. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF48350. SSF48350. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 3 hits.
PS50200. RA. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8WWN8-1 [UniParc]FASTAAdd to Basket

« Hide

MAAPQDLDIA VWLATVHLEQ YADTFRRHGL ATAGAARGLG HEELKQLGIS     50
ATGHRKRILR LLQTGTEEGS LDPKSDSAME PSPSPAPQAQ PPKPVPKPRT 100
VFGGLSGPAT TQRPGLSPAL GGPGVSRSPE PSPRPPPLPT SSSEQSSALN 150
TVEMMPNSIY FGLDSRGRAQ AAQDKAPDSS QISAPTPALR PTTGTVHIMD 200
PGCLYYGVQP VGTPGAPDRR ESRGVCQGRA EHRLSRQDLE AREDAGYASL 250
ELPGDSTLLS PTLETEETSD DLISPYASFS FTADRLTPLL SGWLDKLSPQ 300
GNYVFQRRFV QFNGRSLMYF GSDKDPFPKG VIPLTAIEMT RSSKDNKFQV 350
ITGQRVFVFR TESEAQRDMW CSTLQSCLKE QRLLGHPRPP QPPRPLRTGM 400
LELRGHKAKV FAALSPGELA LYKSEQAFSL GIGICFIELQ GCSVRETKSR 450
SFDLLTPHRC FSFTAESGGA RQSWAAALQE AVTETLSDYE VAEKIWSNRA 500
NRQCADCGSS RPDWAAVNLG VVICKQCAGQ HRALGSGISK VQSLKLDTSV 550
WSNEIVQLFI VLGNDRANRF WAGTLPPGEG LHPDATPGPR GEFISRKYRL 600
GLFRKPHPQY PDHSQLLQAL CAAVARPNLL KNMTQLLCVE AFEGEEPWFP 650
PAPDGSCPGL LPSDPSPGVY NEVVVRATYS GFLYCSPVSN KAGPSPPRRG 700
RDAPPRLWCV LGAALEMFAS ENSPEPLSLI QPQDIVCLGV SPPPTDPGDR 750
FPFSFELILA GGRIQHFGTD GADSLEAWTS AVGKWFSPLS CHQLLGPGLL 800
RLGRLWLRSP SHTAPAPGLW LSGFGLLRGD HLFLCSAPGP GPPAPEDMVH 850
LRRLQEISVV SAADTPDKKE HLVLVETGRT LYLQGEGRLD FTAWNAAIGG 900
AAGGGGTGLQ EQQMSRGDIP IIVDACISFV TQHGLRLEGV YRKGGARARS 950
LRLLAEFRRD ARSVKLRPGE HFVEDVTDTL KRFFRELDDP VTSARLLPRW 1000
REAAELPQKN QRLEKYKDVI GCLPRVNRRT LATLIGHLYR VQKCAALNQM 1050
CTRNLALLFA PSVFQTDGRG EHEVRVLQEL IDGYISVFDI DSDQVAQIDL 1100
EVSLITTWKD VQLSQAGDLI MEVYIEQQLP DNCVTLKVSP TLTAEELTNQ 1150
VLEMRGTAAG MDLWVTFEIR EHGELERPLH PKEKVLEQAL QWCQLPEPCS 1200
ASLLLKKVPL AQAGCLFTGI RRESPRVGLL RCREEPPRLL GSRFQERFFL 1250
LRGRCLLLLK EKKSSKPERE WPLEGAKVYL GIRKKLKPPT PWGFTLILEK 1300
MHLYLSCTDE DEMWDWTTSI LKAQHDDQQP VVLRRHSSSD LARQKFGTMP 1350
LLPIRGDDSG ATLLSANQTL RRLHNRRTLS MFFPMKSSQG SVEEQEELEE 1400
PVYEEPVYEE VGAFPELIQD TSTSFSTTRE WTVKPENPLT SQKSLDQPFL 1450
SKSSTLGQEE RPPEPPPGPP SKSSPQARGS LEEQLLQELS SLILRKGETT 1500
AGLGSPSQPS SPQSPSPTGL PTQTPGFPTQ PPCTSSPPSS QPLT 1544
Length:1,544
Mass (Da):169,844
Last modified:March 1, 2002 - v1
Checksum:i40C03A22591B2B6F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti218 – 2181D → H.
Corresponds to variant rs1031904 [ dbSNP | Ensembl ].
VAR_048330
Natural varianti471 – 4711R → W in a colorectal cancer sample; somatic mutation. 1 Publication
VAR_036180
Natural varianti1085 – 10851I → M in a breast cancer sample; somatic mutation. 1 Publication
VAR_036181
Natural varianti1428 – 14281T → P in a breast cancer sample; somatic mutation. 1 Publication
VAR_036182

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ310567 mRNA. Translation: CAC83946.1.
CH471062 Genomic DNA. Translation: EAW61904.1.
CH471062 Genomic DNA. Translation: EAW61905.1.
CCDSiCCDS4266.1.
PIRiE59431.
RefSeqiNP_071926.4. NM_022481.5.
UniGeneiHs.726187.

Genome annotation databases

EnsembliENST00000239440; ENSP00000239440; ENSG00000120318.
GeneIDi64411.
KEGGihsa:64411.
UCSCiuc003llm.3. human.

Polymorphism databases

DMDMi73619965.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ310567 mRNA. Translation: CAC83946.1 .
CH471062 Genomic DNA. Translation: EAW61904.1 .
CH471062 Genomic DNA. Translation: EAW61905.1 .
CCDSi CCDS4266.1.
PIRi E59431.
RefSeqi NP_071926.4. NM_022481.5.
UniGenei Hs.726187.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2KG5 NMR - A 1-80 [» ]
2LNW NMR - B 1404-1412 [» ]
ProteinModelPortali Q8WWN8.
SMRi Q8WWN8. Positions 1-80, 293-485, 491-653, 906-1100.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 122163. 3 interactions.
IntActi Q8WWN8. 1 interaction.
MINTi MINT-8098880.
STRINGi 9606.ENSP00000239440.

PTM databases

PhosphoSitei Q8WWN8.

Polymorphism databases

DMDMi 73619965.

Proteomic databases

MaxQBi Q8WWN8.
PaxDbi Q8WWN8.
PeptideAtlasi Q8WWN8.
PRIDEi Q8WWN8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000239440 ; ENSP00000239440 ; ENSG00000120318 .
GeneIDi 64411.
KEGGi hsa:64411.
UCSCi uc003llm.3. human.

Organism-specific databases

CTDi 64411.
GeneCardsi GC05M141032.
HGNCi HGNC:24097. ARAP3.
HPAi HPA042887.
MIMi 606647. gene.
neXtProti NX_Q8WWN8.
PharmGKBi PA164715983.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5347.
HOGENOMi HOG000246988.
HOVERGENi HBG069114.
InParanoidi Q8WWN8.
KOi K12490.
OMAi TQLLCVE.
PhylomeDBi Q8WWN8.
TreeFami TF105769.

Enzyme and pathway databases

Reactomei REACT_11051. Rho GTPase cycle.

Miscellaneous databases

ChiTaRSi ARAP3. human.
EvolutionaryTracei Q8WWN8.
GeneWikii CENTD3.
GenomeRNAii 64411.
NextBioi 66374.
PROi Q8WWN8.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8WWN8.
Bgeei Q8WWN8.
CleanExi HS_ARAP3.
Genevestigatori Q8WWN8.

Family and domain databases

Gene3Di 1.10.150.50. 1 hit.
1.10.555.10. 1 hit.
2.30.29.30. 3 hits.
InterProi IPR001164. ArfGAP.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000159. Ras-assoc.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF01412. ArfGap. 1 hit.
PF00169. PH. 2 hits.
PF00788. RA. 1 hit.
PF00620. RhoGAP. 1 hit.
PF07647. SAM_2. 1 hit.
[Graphical view ]
PRINTSi PR00405. REVINTRACTNG.
SMARTi SM00105. ArfGap. 1 hit.
SM00233. PH. 5 hits.
SM00324. RhoGAP. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view ]
SUPFAMi SSF47769. SSF47769. 1 hit.
SSF48350. SSF48350. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEi PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 3 hits.
PS50200. RA. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF 307-ARG-ARG-308, FUNCTION, INTERACTION WITH PHOSPHATIDYLINOSITOL 3-PHOSPHATE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "GTPase signaling: bridging the GAP between ARF and Rho."
    Santy L.C., Casanova J.E.
    Curr. Biol. 12:R360-R362(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  4. "EST-based genome-wide gene inactivation identifies ARAP3 as a host protein affecting cellular susceptibility to anthrax toxin."
    Lu Q., Wei W., Kowalski P.E., Chang A.C.Y., Cohen S.N.
    Proc. Natl. Acad. Sci. U.S.A. 101:17246-17251(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN THE INTERNALIZATION OF ANTHRAX TOXIN.
  5. "The PI3K effector Arap3 interacts with the PI(3,4,5)P3 phosphatase SHIP2 in a SAM domain-dependent manner."
    Raaijmakers J.H., Deneubourg L., Rehmann H., de Koning J., Zhang Z., Krugmann S., Erneux C., Bos J.L.
    Cell. Signal. 19:1249-1257(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH INPPL1.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1444, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1444 AND SER-1480, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "The Sam domain of the lipid phosphatase Ship2 adopts a common model to interact with Arap3-Sam and EphA2-Sam."
    Leone M., Cellitti J., Pellecchia M.
    BMC Struct. Biol. 9:59-59(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 1-80, INTERACTION WITH INPPL1.
  10. Cited for: VARIANTS [LARGE SCALE ANALYSIS] TRP-471; MET-1085 AND PRO-1428.

Entry informationi

Entry nameiARAP3_HUMAN
AccessioniPrimary (citable) accession number: Q8WWN8
Secondary accession number(s): D3DQE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: March 1, 2002
Last modified: September 3, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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