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Q8WWL7 (CCNB3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G2/mitotic-specific cyclin-B3
Gene names
Name:CCNB3
Synonyms:CYCB3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1395 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cyclins are positive regulatory subunits of the cyclin-dependent kinases (CDKs), and thereby play an essential role in the control of the cell cycle, notably via their destruction during cell division. Its tissue specificity suggest that it may be required during early meiotic prophase I. Ref.1

Subunit structure

Interacts with CDK2 kinase. Ref.1

Subcellular location

Nucleus Ref.1.

Tissue specificity

Testis specific. In testis, it is expressed in developing germ cells, but not in Leydig cells. Weakly or not expressed in other tissues. Ref.1 Ref.2

Domain

The N-terminal destruction box (D-box) probably acts as a recognition signal for degradation via the ubiquitin-proteasome pathway By similarity. Ref.1

Post-translational modification

Ubiquitinated Probable. Ubiquitination leads to its degradation during anaphase entry, after degradation of CCNB1.

Sequence similarities

Belongs to the cyclin family. Cyclin AB subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CDK2A8UKE62EBI-767764,EBI-767796From a different organism.
Plk4Q647024EBI-767764,EBI-2552433From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8WWL7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8WWL7-2)

Also known as: Variant 1;

The sequence of this isoform differs from the canonical sequence as follows:
     69-1172: Missing.
Isoform 3 (identifier: Q8WWL7-3)

Also known as: Variant 2;

The sequence of this isoform differs from the canonical sequence as follows:
     112-1395: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13951395G2/mitotic-specific cyclin-B3
PRO_0000080373

Regions

Motif60 – 689D-box

Natural variations

Alternative sequence69 – 11721104Missing in isoform 2.
VSP_010514
Alternative sequence112 – 13951284Missing in isoform 3.
VSP_010515
Natural variant5971K → T in a colorectal cancer sample; somatic mutation. Ref.7
VAR_036580
Natural variant10011G → R.
Corresponds to variant rs6614336 [ dbSNP | Ensembl ].
VAR_047027

Experimental info

Mutagenesis601R → A in cycB3XA; prevents its destruction after completion of anaphase; when associated with A-63 and A-68.
Mutagenesis631F → A in cycB3XA; prevents its destruction after completion of anaphase; when associated with A-60 and A-68.
Mutagenesis681N → A in cycB3XA; prevents its destruction after completion of anaphase; when associated with A-60 and A-63.
Sequence conflict2951K → R in CAC40024. Ref.2
Sequence conflict3311S → F in CAC94915. Ref.1
Sequence conflict3961S → P in CAC40024. Ref.2
Sequence conflict4931R → K in CAC40024. Ref.2
Sequence conflict5731N → S in CAC40024. Ref.2
Sequence conflict5971K → E in CAC40024. Ref.2
Sequence conflict7741E → K in CAC94915. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 14, 2008. Version 2.
Checksum: 454552BC5A67E34E

FASTA1,395157,916
        10         20         30         40         50         60 
MLLPLPPQSS KPVPKKSQSS KIVPSHHDPS EKTGENCQTK ISPSSLQESP SSLQGALKKR 

        70         80         90        100        110        120 
SAFEDLTNAS QCQPVQPKKE ANKEFVKVVS KKINRNTHAL GLAKKNKRNL KWHKLEVTPV 

       130        140        150        160        170        180 
VASTTVVPNI MEKPLILDIS TTSKTPNTEE ASLFRKPLVL KEEPTIEDET LINKSLSLKK 

       190        200        210        220        230        240 
CSNHEEVSLL EKLQPLQEES DSDDAFVIEP MTFKKTHKTE EAAITKKTLS LKKKMCASQR 

       250        260        270        280        290        300 
KQSCQEESLA VQDVNMEEDS FFMESMSFKK KPKTEESIPT HKLSSLKKKC TIYGKICHFR 

       310        320        330        340        350        360 
KPPVLQTTIC GAMSSIKKPT TEKETLFQEL SVLQEKHTTE HEMSILKKSL ALQKTNFKED 

       370        380        390        400        410        420 
SLVKESLAFK KKPSTEEAIM MPVILKEQCM TEGKRSRLKP LVLQEITSGE KSLIMKPLSI 

       430        440        450        460        470        480 
KEKPSTEKES FSQEPSALQK KHTTQEEVSI LKEPSSLLKS PTEESPFDEA LAFTKKCTIE 

       490        500        510        520        530        540 
EAPPTKKPLI LKRKHATQGT MSHLKKPLIL QTTSGEKSLI KEPLPFKEEK VSLKKKCTTQ 

       550        560        570        580        590        600 
EMMSICPELL DFQDMIGEDK NSFFMEPMSF RKNPTTEETV LTKTSLSLQE KKITQGKMSH 

       610        620        630        640        650        660 
LKKPLVLQKI TSEEESFYKK LLPFKMKSTT EEKFLSQEPS ALKEKHTTLQ EVSLSKESLA 

       670        680        690        700        710        720 
IQEKATTEEE FSQELFSLHV KHTNKSGSLF QEALVLQEKT DAEEDSLKNL LALQEKSTME 

       730        740        750        760        770        780 
EESLINKLLA LKEELSAEAA TNIQTQLSLK KKSTSHGKVF FLKKQLALNE TINEEEFLNK 

       790        800        810        820        830        840 
QPLALEGYPS IAEGETLFKK LLAMQEEPSI EKEAVLKEPT IDTEAHFKEP LALQEEPSTE 

       850        860        870        880        890        900 
KEAVLKEPSV DTEAHFKETL ALQEKPSIEQ EALFKRHSAL WEKPSTEKET IFKESLDLQE 

       910        920        930        940        950        960 
KPSIKKETLL KKPLALKMST INEAVLFEDM IALNEKPTTG KELSFKEPLA LQESPTYKED 

       970        980        990       1000       1010       1020 
TFLKTLLVPQ VGTSPNVSST APESITSKSS IATMTSVGKS GTINEAFLFE DMITLNEKPT 

      1030       1040       1050       1060       1070       1080 
TGKELSFKEP LALQESPTCK EDTFLETFLI PQIGTSPYVF STTPESITEK SSIATMTSVG 

      1090       1100       1110       1120       1130       1140 
KSRTTTESSA CESASDKPVS PQAKGTPKEI TPREDIDEDS SDPSFNPMYA KEIFSYMKER 

      1150       1160       1170       1180       1190       1200 
EEQFILTDYM NRQIEITSDM RAILVDWLVE VQVSFEMTHE TLYLAVKLVD LYLMKAVCKK 

      1210       1220       1230       1240       1250       1260 
DKLQLLGATA FMIAAKFEEH NSPRVDDFVY ICDDNYQRSE VLSMEINILN VLKCDINIPI 

      1270       1280       1290       1300       1310       1320 
AYHFLRRYAR CIHTNMKTLT LSRYICEMTL QEYHYVQEKA SKLAAASLLL ALYMKKLGYW 

      1330       1340       1350       1360       1370       1380 
VPFLEHYSGY SISELHPLVR QLNKLLTFSS YDSLKAVYYK YSHPVFFEVA KIPALDMLKL 

      1390 
EEILNCDCEA QGLVL 

« Hide

Isoform 2 (Variant 1) [UniParc].

Checksum: CC00FC3079181B7E
Show »

FASTA29133,275
Isoform 3 (Variant 2) [UniParc].

Checksum: 0D8F2ACB1016D226
Show »

FASTA11112,195

References

« Hide 'large scale' references
[1]"Characterization and expression of mammalian cyclin b3, a prepachytene meiotic cyclin."
Nguyen T.B., Manova K., Capodieci P., Lindon C., Bottega S., Wang X.-Y., Refik-Rogers J., Pines J., Wolgemuth D.J., Koff A.
J. Biol. Chem. 277:41960-41969(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, DOMAIN, TISSUE SPECIFICITY, MUTANT CYCB3XA, INTERACTION WITH CDK2.
Tissue: Testis.
[2]"Molecular cloning, gene localization, and structure of human cyclin B3."
Lozano J.-C., Perret E., Schatt P., Arnould C., Peaucellier G., Picard A.
Biochem. Biophys. Res. Commun. 291:406-413(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
Tissue: Placenta.
[3]"The DNA sequence of the human X chromosome."
Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. expand/collapse author list , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1249-1395 (ISOFORM 1).
Tissue: Testis.
[6]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] THR-597.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ416458 mRNA. Translation: CAC94915.1.
AJ314764 mRNA. Translation: CAC40024.1.
AJ314765 mRNA. Translation: CAC40025.1.
AJ314766 mRNA. Translation: CAC40026.1.
AL591367, BX323840 Genomic DNA. Translation: CAI41320.1.
AL591367, BX323840 Genomic DNA. Translation: CAI41321.1.
BX323840, AL591367 Genomic DNA. Translation: CAI43220.1.
BX323840, AL591367 Genomic DNA. Translation: CAI43221.1.
CH471180 Genomic DNA. Translation: EAW89922.1.
CH471180 Genomic DNA. Translation: EAW89923.1.
AL137550 mRNA. Translation: CAB70806.1.
CCDSCCDS14331.1. [Q8WWL7-1]
CCDS14332.1. [Q8WWL7-2]
PIRT46391.
RefSeqNP_149020.2. NM_033031.2. [Q8WWL7-1]
NP_391990.1. NM_033670.2. [Q8WWL7-2]
UniGeneHs.130310.
Hs.722420.
Hs.722421.
Hs.740165.

3D structure databases

ProteinModelPortalQ8WWL7.
SMRQ8WWL7. Positions 1127-1370.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid124523. 2 interactions.
IntActQ8WWL7. 3 interactions.
MINTMINT-4054013.
STRING9606.ENSP00000276014.

Chemistry

BindingDBQ8WWL7.
ChEMBLCHEMBL2094127.

PTM databases

PhosphoSiteQ8WWL7.

Polymorphism databases

DMDM209572596.

Proteomic databases

PaxDbQ8WWL7.
PRIDEQ8WWL7.

Protocols and materials databases

DNASU85417.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000276014; ENSP00000276014; ENSG00000147082. [Q8WWL7-1]
ENST00000348603; ENSP00000338682; ENSG00000147082. [Q8WWL7-2]
ENST00000376038; ENSP00000365206; ENSG00000147082. [Q8WWL7-2]
ENST00000376042; ENSP00000365210; ENSG00000147082. [Q8WWL7-1]
ENST00000476167; ENSP00000431645; ENSG00000147082. [Q8WWL7-3]
ENST00000593643; ENSP00000471254; ENSG00000268264. [Q8WWL7-2]
ENST00000594292; ENSP00000472548; ENSG00000268264. [Q8WWL7-1]
ENST00000595350; ENSP00000470817; ENSG00000268264. [Q8WWL7-3]
ENST00000596541; ENSP00000471997; ENSG00000269438. [Q8WWL7-1]
ENST00000597115; ENSP00000469069; ENSG00000268264. [Q8WWL7-1]
ENST00000598186; ENSP00000472565; ENSG00000269438. [Q8WWL7-3]
ENST00000598566; ENSP00000471440; ENSG00000268264. [Q8WWL7-2]
ENST00000599832; ENSP00000470722; ENSG00000269438. [Q8WWL7-1]
ENST00000601442; ENSP00000472573; ENSG00000269438. [Q8WWL7-2]
ENST00000602116; ENSP00000468921; ENSG00000269438. [Q8WWL7-2]
GeneID85417.
KEGGhsa:85417.
UCSCuc004dox.4. human. [Q8WWL7-1]
uc004doz.3. human. [Q8WWL7-2]

Organism-specific databases

CTD85417.
GeneCardsGC0XP049967.
HGNCHGNC:18709. CCNB3.
HPAHPA000496.
MIM300456. gene.
neXtProtNX_Q8WWL7.
PharmGKBPA38653.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5024.
HOGENOMHOG000231355.
HOVERGENHBG050835.
InParanoidQ8WWL7.
KOK05868.
OMANEMISEP.
OrthoDBEOG7P2XST.
PhylomeDBQ8WWL7.
TreeFamTF101001.

Enzyme and pathway databases

SignaLinkQ8WWL7.

Gene expression databases

ArrayExpressQ8WWL7.
BgeeQ8WWL7.
CleanExHS_CCNB3.
GenevestigatorQ8WWL7.

Family and domain databases

Gene3D1.10.472.10. 2 hits.
InterProIPR013763. Cyclin-like.
IPR015452. Cyclin_B3.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
[Graphical view]
PANTHERPTHR10177:SF22. PTHR10177:SF22. 1 hit.
PfamPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMSSF47954. SSF47954. 2 hits.
ProtoNetSearch...

Other

GenomeRNAi85417.
NextBio75998.
PROQ8WWL7.
SOURCESearch...

Entry information

Entry nameCCNB3_HUMAN
AccessionPrimary (citable) accession number: Q8WWL7
Secondary accession number(s): B1AQI5 expand/collapse secondary AC list , B1AQI6, Q96SB5, Q96SB6, Q96SB7, Q9NT38
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 14, 2008
Last modified: July 9, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM