Q8WWL7 (CCNB3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G2/mitotic-specific cyclin-B3

Gene names

Name:	CCNB3
Synonyms:	CYCB3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1395 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cyclins are positive regulatory subunits of the cyclin-dependent kinases (CDKs), and thereby play an essential role in the control of the cell cycle, notably via their destruction during cell division. Its tissue specificity suggest that it may be required during early meiotic prophase I. Ref.1
Subunit structure	Interacts with CDK2 kinase. Ref.1
Subcellular location	Nucleus Ref.1.
Tissue specificity	Testis specific. In testis, it is expressed in developing germ cells, but not in Leydig cells. Weakly or not expressed in other tissues. Ref.1 Ref.2
Domain	The N-terminal destruction box (D-box) probably acts as a recognition signal for degradation via the ubiquitin-proteasome pathway By similarity. Ref.1
Post-translational modification	Ubiquitinated Probable. Ubiquitination leads to its degradation during anaphase entry, after degradation of CCNB1.
Sequence similarities	Belongs to the cyclin family. Cyclin AB subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Meiosis
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Cyclin
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW meiosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of G2/M transition of mitotic cell cycle Inferred from electronic annotation. Source: InterPro regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleus Inferred from direct assay. Source: HPA
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDK2	A8UKE6	2	EBI-767764,EBI-767796	From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8WWL7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8WWL7-2)

Also known as: Variant 1;

The sequence of this isoform differs from the canonical sequence as follows:
     69-1172: Missing.

Isoform 3 (identifier: Q8WWL7-3)

Also known as: Variant 2;

The sequence of this isoform differs from the canonical sequence as follows:
     112-1395: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1395	1395	G2/mitotic-specific cyclin-B3		PRO_0000080373
Regions
	Motif	60 – 68	9	D-box
Natural variations
	Alternative sequence	69 – 1172	1104	Missing in isoform 2.		VSP_010514
	Alternative sequence	112 – 1395	1284	Missing in isoform 3.		VSP_010515
	Natural variant	597	1	K → T in a colorectal cancer sample; somatic mutation. Ref.6		VAR_036580
	Natural variant	1001	1	G → R. Corresponds to variant rs6614336 [ dbSNP | Ensembl ].		VAR_047027
Experimental info
	Mutagenesis	60	1	R → A in cycB3XA; prevents its destruction after completion of anaphase; when associated with A-63 and A-68.
	Mutagenesis	63	1	F → A in cycB3XA; prevents its destruction after completion of anaphase; when associated with A-60 and A-68.
	Mutagenesis	68	1	N → A in cycB3XA; prevents its destruction after completion of anaphase; when associated with A-60 and A-63.
	Sequence conflict	295	1	K → R in CAC40024. Ref.2
	Sequence conflict	331	1	S → F in CAC94915. Ref.1
	Sequence conflict	396	1	S → P in CAC40024. Ref.2
	Sequence conflict	493	1	R → K in CAC40024. Ref.2
	Sequence conflict	573	1	N → S in CAC40024. Ref.2
	Sequence conflict	597	1	K → E in CAC40024. Ref.2
	Sequence conflict	774	1	E → K in CAC94915. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 14, 2008. Version 2. Checksum: 454552BC5A67E34E	FASTA	1,395	157,916
	10 20 30 40 50 60 MLLPLPPQSS KPVPKKSQSS KIVPSHHDPS EKTGENCQTK ISPSSLQESP SSLQGALKKR 70 80 90 100 110 120 SAFEDLTNAS QCQPVQPKKE ANKEFVKVVS KKINRNTHAL GLAKKNKRNL KWHKLEVTPV 130 140 150 160 170 180 VASTTVVPNI MEKPLILDIS TTSKTPNTEE ASLFRKPLVL KEEPTIEDET LINKSLSLKK 190 200 210 220 230 240 CSNHEEVSLL EKLQPLQEES DSDDAFVIEP MTFKKTHKTE EAAITKKTLS LKKKMCASQR 250 260 270 280 290 300 KQSCQEESLA VQDVNMEEDS FFMESMSFKK KPKTEESIPT HKLSSLKKKC TIYGKICHFR 310 320 330 340 350 360 KPPVLQTTIC GAMSSIKKPT TEKETLFQEL SVLQEKHTTE HEMSILKKSL ALQKTNFKED 370 380 390 400 410 420 SLVKESLAFK KKPSTEEAIM MPVILKEQCM TEGKRSRLKP LVLQEITSGE KSLIMKPLSI 430 440 450 460 470 480 KEKPSTEKES FSQEPSALQK KHTTQEEVSI LKEPSSLLKS PTEESPFDEA LAFTKKCTIE 490 500 510 520 530 540 EAPPTKKPLI LKRKHATQGT MSHLKKPLIL QTTSGEKSLI KEPLPFKEEK VSLKKKCTTQ 550 560 570 580 590 600 EMMSICPELL DFQDMIGEDK NSFFMEPMSF RKNPTTEETV LTKTSLSLQE KKITQGKMSH 610 620 630 640 650 660 LKKPLVLQKI TSEEESFYKK LLPFKMKSTT EEKFLSQEPS ALKEKHTTLQ EVSLSKESLA 670 680 690 700 710 720 IQEKATTEEE FSQELFSLHV KHTNKSGSLF QEALVLQEKT DAEEDSLKNL LALQEKSTME 730 740 750 760 770 780 EESLINKLLA LKEELSAEAA TNIQTQLSLK KKSTSHGKVF FLKKQLALNE TINEEEFLNK 790 800 810 820 830 840 QPLALEGYPS IAEGETLFKK LLAMQEEPSI EKEAVLKEPT IDTEAHFKEP LALQEEPSTE 850 860 870 880 890 900 KEAVLKEPSV DTEAHFKETL ALQEKPSIEQ EALFKRHSAL WEKPSTEKET IFKESLDLQE 910 920 930 940 950 960 KPSIKKETLL KKPLALKMST INEAVLFEDM IALNEKPTTG KELSFKEPLA LQESPTYKED 970 980 990 1000 1010 1020 TFLKTLLVPQ VGTSPNVSST APESITSKSS IATMTSVGKS GTINEAFLFE DMITLNEKPT 1030 1040 1050 1060 1070 1080 TGKELSFKEP LALQESPTCK EDTFLETFLI PQIGTSPYVF STTPESITEK SSIATMTSVG 1090 1100 1110 1120 1130 1140 KSRTTTESSA CESASDKPVS PQAKGTPKEI TPREDIDEDS SDPSFNPMYA KEIFSYMKER 1150 1160 1170 1180 1190 1200 EEQFILTDYM NRQIEITSDM RAILVDWLVE VQVSFEMTHE TLYLAVKLVD LYLMKAVCKK 1210 1220 1230 1240 1250 1260 DKLQLLGATA FMIAAKFEEH NSPRVDDFVY ICDDNYQRSE VLSMEINILN VLKCDINIPI 1270 1280 1290 1300 1310 1320 AYHFLRRYAR CIHTNMKTLT LSRYICEMTL QEYHYVQEKA SKLAAASLLL ALYMKKLGYW 1330 1340 1350 1360 1370 1380 VPFLEHYSGY SISELHPLVR QLNKLLTFSS YDSLKAVYYK YSHPVFFEVA KIPALDMLKL 1390 EEILNCDCEA QGLVL
	Isoform 2 (Variant 1) [UniParc]. Checksum: CC00FC3079181B7E	FASTA	291	33,275
	10 20 30 40 50 60 MLLPLPPQSS KPVPKKSQSS KIVPSHHDPS EKTGENCQTK ISPSSLQESP SSLQGALKKR 70 80 90 100 110 120 SAFEDLTNVS FEMTHETLYL AVKLVDLYLM KAVCKKDKLQ LLGATAFMIA AKFEEHNSPR 130 140 150 160 170 180 VDDFVYICDD NYQRSEVLSM EINILNVLKC DINIPIAYHF LRRYARCIHT NMKTLTLSRY 190 200 210 220 230 240 ICEMTLQEYH YVQEKASKLA AASLLLALYM KKLGYWVPFL EHYSGYSISE LHPLVRQLNK 250 260 270 280 290 LLTFSSYDSL KAVYYKYSHP VFFEVAKIPA LDMLKLEEIL NCDCEAQGLV L
	Isoform 3 (Variant 2) [UniParc]. Checksum: 0D8F2ACB1016D226	FASTA	111	12,195
	10 20 30 40 50 60 MLLPLPPQSS KPVPKKSQSS KIVPSHHDPS EKTGENCQTK ISPSSLQESP SSLQGALKKR 70 80 90 100 110 SAFEDLTNAS QCQPVQPKKE ANKEFVKVVS KKINRNTHAL GLAKKNKRNL K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization and expression of mammalian cyclin b3, a prepachytene meiotic cyclin." Nguyen T.B., Manova K., Capodieci P., Lindon C., Bottega S., Wang X.-Y., Refik-Rogers J., Pines J., Wolgemuth D.J., Koff A. J. Biol. Chem. 277:41960-41969(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, DOMAIN, TISSUE SPECIFICITY, MUTANT CYCB3XA, INTERACTION WITH CDK2. Tissue: Testis.
[2]	"Molecular cloning, gene localization, and structure of human cyclin B3." Lozano J.-C., Perret E., Schatt P., Arnould C., Peaucellier G., Picard A. Biochem. Biophys. Res. Commun. 291:406-413(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), ALTERNATIVE SPLICING, TISSUE SPECIFICITY. Tissue: Placenta.
[3]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1249-1395 (ISOFORM 1). Tissue: Testis.
[6]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] THR-597.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ416458 mRNA. Translation: CAC94915.1. AJ314764 mRNA. Translation: CAC40024.1. AJ314765 mRNA. Translation: CAC40025.1. AJ314766 mRNA. Translation: CAC40026.1. AL591367, BX323840 Genomic DNA. Translation: CAI41320.1. AL591367, BX323840 Genomic DNA. Translation: CAI41321.1. BX323840, AL591367 Genomic DNA. Translation: CAI43220.1. BX323840, AL591367 Genomic DNA. Translation: CAI43221.1. CH471180 Genomic DNA. Translation: EAW89922.1. CH471180 Genomic DNA. Translation: EAW89923.1. AL137550 mRNA. Translation: CAB70806.1.
IPI	IPI00066473. IPI00070367. IPI00152849.
PIR	T46391.
RefSeq	NP_149020.2. NM_033031.2. NP_391990.1. NM_033670.2.
UniGene	Hs.130310. Hs.722420. Hs.722421. Hs.740165.
3D structure databases
ProteinModelPortal	Q8WWL7.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8WWL7. 1 interaction.
MINT	MINT-4054013.
STRING	9606.ENSP00000276014.
PTM databases
PhosphoSite	Q8WWL7.
Polymorphism databases
DMDM	209572596.
Proteomic databases
PaxDb	Q8WWL7.
PRIDE	Q8WWL7.
Protocols and materials databases
DNASU	85417.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000276014; ENSP00000276014; ENSG00000147082. ENST00000348603; ENSP00000338682; ENSG00000147082. ENST00000376038; ENSP00000365206; ENSG00000147082. ENST00000376042; ENSP00000365210; ENSG00000147082. ENST00000476167; ENSP00000431645; ENSG00000147082. ENST00000593643; ENSP00000471254; ENSG00000268264. ENST00000594292; ENSP00000472548; ENSG00000268264. ENST00000595350; ENSP00000470817; ENSG00000268264. ENST00000596541; ENSP00000471997; ENSG00000269438. ENST00000597115; ENSP00000469069; ENSG00000268264. ENST00000598186; ENSP00000472565; ENSG00000269438. ENST00000598566; ENSP00000471440; ENSG00000268264. ENST00000599832; ENSP00000470722; ENSG00000269438. ENST00000601442; ENSP00000472573; ENSG00000269438. ENST00000602116; ENSP00000468921; ENSG00000269438.
GeneID	85417.
KEGG	hsa:85417.
UCSC	uc004dox.4. human. uc004doz.3. human.
Organism-specific databases
CTD	85417.
GeneCards	GC0XP049967.
HGNC	HGNC:18709. CCNB3.
HPA	HPA000496.
MIM	300456. gene.
neXtProt	NX_Q8WWL7.
PharmGKB	PA38653.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5024.
HOGENOM	HOG000231355.
HOVERGEN	HBG050835.
InParanoid	Q8WWL7.
KO	K05868.
OMA	SLKKKCT.
OrthoDB	EOG4JT04W.
PhylomeDB	Q8WWL7.
Enzyme and pathway databases
SignaLink	Q8WWL7.
Gene expression databases
ArrayExpress	Q8WWL7.
Bgee	Q8WWL7.
CleanEx	HS_CCNB3.
Genevestigator	Q8WWL7.
GermOnline	ENSG00000147082. Homo sapiens.
Family and domain databases
Gene3D	1.10.472.10. 2 hits.
InterPro	IPR013763. Cyclin-like. IPR015452. Cyclin_B3. IPR004367. Cyclin_C-dom. IPR006671. Cyclin_N. [Graphical view]
PANTHER	PTHR10177:SF22. PTHR10177:SF22. 1 hit.
Pfam	PF02984. Cyclin_C. 1 hit. PF00134. Cyclin_N. 1 hit. [Graphical view]
SMART	SM00385. CYCLIN. 2 hits. [Graphical view]
SUPFAM	SSF47954. Cyclin_like. 2 hits.
PROSITE	PS00292. CYCLINS. False negative. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q8WWL7.
ChEMBL	CHEMBL3606.
GenomeRNAi	85417.
NextBio	75998.
SOURCE	Search...

Entry information

Entry name

CCNB3_HUMAN

Accession

Primary (citable) accession number: Q8WWL7
Secondary accession number(s): B1AQI5 , B1AQI6, Q96SB5, Q96SB6, Q96SB7, Q9NT38

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	October 14, 2008
Last modified:	May 29, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.