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Protein

Protein spire homolog 2

Gene

SPIRE2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning and asymmetric cell division during meiosis. Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

BioCyciZFISH:G66-31190-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein spire homolog 2
Short name:
Spir-2
Gene namesi
Name:SPIRE2
Synonyms:KIAA1832, SPIR2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:30623. SPIRE2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000204991.
PharmGKBiPA134926582.

Polymorphism and mutation databases

BioMutaiSPIRE2.
DMDMi296452951.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003200231 – 714Protein spire homolog 2Add BLAST714

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei371PhosphoserineCombined sources1
Modified residuei440PhosphoserineCombined sources1
Modified residuei442PhosphoserineCombined sources1
Modified residuei476PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8WWL2.
PaxDbiQ8WWL2.
PeptideAtlasiQ8WWL2.
PRIDEiQ8WWL2.

PTM databases

iPTMnetiQ8WWL2.
PhosphoSitePlusiQ8WWL2.

Expressioni

Gene expression databases

BgeeiENSG00000204991.
CleanExiHS_SPIRE2.
ExpressionAtlasiQ8WWL2. baseline and differential.
GenevisibleiQ8WWL2. HS.

Organism-specific databases

HPAiHPA041413.
HPA049415.

Interactioni

Protein-protein interaction databases

BioGridi124102. 7 interactors.
IntActiQ8WWL2. 5 interactors.
STRINGi9606.ENSP00000367494.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JCYX-ray1.80B401-427[»]
ProteinModelPortaliQ8WWL2.
SMRiQ8WWL2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 203KINDPROSITE-ProRule annotationAdd BLAST182
Domaini248 – 262WH2 1PROSITE-ProRule annotationAdd BLAST15
Domaini278 – 296WH2 2PROSITE-ProRule annotationAdd BLAST19
Domaini342 – 359WH2 3PROSITE-ProRule annotationAdd BLAST18

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni534 – 554Spir-boxAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi572 – 599Cys-richAdd BLAST28

Domaini

Binds to actin monomers via the WH2 domain.By similarity
The Spir-box targets binding to intracellular membrane structures.By similarity

Sequence similaritiesi

Belongs to the spire family.Curated
Contains 1 KIND domain.PROSITE-ProRule annotation
Contains 3 WH2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IK9H. Eukaryota.
ENOG4111E0J. LUCA.
GeneTreeiENSGT00390000003058.
HOGENOMiHOG000013039.
HOVERGENiHBG058898.
InParanoidiQ8WWL2.
KOiK02098.
OMAiCGAADEG.
OrthoDBiEOG091G0EAQ.
PhylomeDBiQ8WWL2.
TreeFamiTF326239.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR011019. KIND_dom.
IPR029901. Spire.
IPR029904. Spire2.
IPR003124. WH2_dom.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR21345. PTHR21345. 2 hits.
PTHR21345:SF5. PTHR21345:SF5. 2 hits.
PfamiPF16474. KIND. 1 hit.
[Graphical view]
SMARTiSM00750. KIND. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS51377. KIND. 1 hit.
PS51082. WH2. 3 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WWL2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARAGSCGGA AAGAGRPEPW ELSLEEVLKA YEQPLNEEQA WAVCFQGCRG
60 70 80 90 100
LRGSPGRRLR DTGDLLLRGD GSVGAREPEA AEPATMVVPL ASSEAQTVQS
110 120 130 140 150
LGFAIYRALD WGLDESEERE LSPQLERLID LMANNDSEDS GCGAADEGYG
160 170 180 190 200
GPEEEEEAEG VPRSVRTFAQ AMRLCAARLT DPRGAQAHYQ AVCRALFVET
210 220 230 240 250
LELRAFLARV REAKEMLQKL REDEPHLETP RAELDSLGHT DWARLWVQLM
260 270 280 290 300
RELRRGVKLK KVQEQEFNPL PTEFQLTPFE MLMQDIRARN YKLRKVMVDG
310 320 330 340 350
DIPPRVKKDA HELILDFIRS RPPLKQVSER RLRPLPPKQR SLHEKILEEI
360 370 380 390 400
KQERRLRPVR GEGWAARGFG SLPCILNACS GDAKSTSCIN LSVTDAGGSA
410 420 430 440 450
QRPRPRVLLK APTLAEMEEM NTSEEEESPC GEVTLKRDRS FSEHDLAQLR
460 470 480 490 500
SEVASGLQSA THPPGGTEPP RPRAGSAHVW RPGSRDQGTC PASVSDPSHP
510 520 530 540 550
LLSNRGSSGD RPEASMTPDA KHLWLEFSHP VESLALTVEE VMDVRRVLVK
560 570 580 590 600
AEMEKFLQNK ELFSSLKKGK ICCCCRAKFP LFSWPPSCLF CKRAVCTSCS
610 620 630 640 650
IKMKMPSKKF GHIPVYTLGF ESPQRVSAAK TAPIQRRDIF QSLQGPQWQS
660 670 680 690 700
VEEAFPHIYS HGCVLKDVCS ECTSFVADVV RSSRKSVDVL NTTPRRSRQT
710
QSLYIPNTRT LDFK
Length:714
Mass (Da):79,671
Last modified:May 18, 2010 - v3
Checksum:i0999F9D5DE6A29CD
GO
Isoform 2 (identifier: Q8WWL2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     594-641: Missing.

Show »
Length:666
Mass (Da):74,333
Checksum:i855ED63B0D0D5C1C
GO
Isoform 3 (identifier: Q8WWL2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.
     49-95: RGLRGSPGRR...MVVPLASSEA → MSCLCLGLLW...VPPSDRAPVP

Show »
Length:666
Mass (Da):74,632
Checksum:i6C27E30D5E3B74AC
GO
Isoform 4 (identifier: Q8WWL2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-131: Missing.
     296-297: Missing.

Show »
Length:581
Mass (Da):65,375
Checksum:iC1BD44D6B34746E3
GO

Sequence cautioni

The sequence BAB47461 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAD19439 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41W → L in CAD19439 (Ref. 1) Curated1
Sequence conflicti216M → T in CAD39070 (PubMed:17974005).Curated1
Sequence conflicti307K → E in BAB47461 (PubMed:11347906).Curated1
Sequence conflicti711L → F in AAI39733 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0315681 – 131Missing in isoform 4. 1 PublicationAdd BLAST131
Alternative sequenceiVSP_0315691 – 48Missing in isoform 3. 1 PublicationAdd BLAST48
Alternative sequenceiVSP_03157049 – 95RGLRG…ASSEA → MSCLCLGLLWTDSCCLPGRV LGPLHPPAAFSPPHPPAVPP SDRAPVP in isoform 3. 1 PublicationAdd BLAST47
Alternative sequenceiVSP_031571296 – 297Missing in isoform 4. 1 Publication2
Alternative sequenceiVSP_031572594 – 641Missing in isoform 2. 1 PublicationAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ422077 mRNA. Translation: CAD19439.1. Different initiation.
AB058735 mRNA. Translation: BAB47461.1. Different initiation.
BC063706 mRNA. Translation: AAH63706.1.
BC111030 mRNA. Translation: AAI11031.1.
BC139732 mRNA. Translation: AAI39733.1.
AL834408 mRNA. Translation: CAD39070.1.
CCDSiCCDS32516.1. [Q8WWL2-1]
RefSeqiNP_115827.1. NM_032451.1. [Q8WWL2-1]
UniGeneiHs.461786.

Genome annotation databases

EnsembliENST00000378247; ENSP00000367494; ENSG00000204991. [Q8WWL2-1]
ENST00000393062; ENSP00000376782; ENSG00000204991. [Q8WWL2-2]
GeneIDi84501.
KEGGihsa:84501.
UCSCiuc002foz.2. human. [Q8WWL2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ422077 mRNA. Translation: CAD19439.1. Different initiation.
AB058735 mRNA. Translation: BAB47461.1. Different initiation.
BC063706 mRNA. Translation: AAH63706.1.
BC111030 mRNA. Translation: AAI11031.1.
BC139732 mRNA. Translation: AAI39733.1.
AL834408 mRNA. Translation: CAD39070.1.
CCDSiCCDS32516.1. [Q8WWL2-1]
RefSeqiNP_115827.1. NM_032451.1. [Q8WWL2-1]
UniGeneiHs.461786.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JCYX-ray1.80B401-427[»]
ProteinModelPortaliQ8WWL2.
SMRiQ8WWL2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124102. 7 interactors.
IntActiQ8WWL2. 5 interactors.
STRINGi9606.ENSP00000367494.

PTM databases

iPTMnetiQ8WWL2.
PhosphoSitePlusiQ8WWL2.

Polymorphism and mutation databases

BioMutaiSPIRE2.
DMDMi296452951.

Proteomic databases

MaxQBiQ8WWL2.
PaxDbiQ8WWL2.
PeptideAtlasiQ8WWL2.
PRIDEiQ8WWL2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000378247; ENSP00000367494; ENSG00000204991. [Q8WWL2-1]
ENST00000393062; ENSP00000376782; ENSG00000204991. [Q8WWL2-2]
GeneIDi84501.
KEGGihsa:84501.
UCSCiuc002foz.2. human. [Q8WWL2-1]

Organism-specific databases

CTDi84501.
GeneCardsiSPIRE2.
H-InvDBHIX0013371.
HGNCiHGNC:30623. SPIRE2.
HPAiHPA041413.
HPA049415.
MIMi609217. gene.
neXtProtiNX_Q8WWL2.
OpenTargetsiENSG00000204991.
PharmGKBiPA134926582.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IK9H. Eukaryota.
ENOG4111E0J. LUCA.
GeneTreeiENSGT00390000003058.
HOGENOMiHOG000013039.
HOVERGENiHBG058898.
InParanoidiQ8WWL2.
KOiK02098.
OMAiCGAADEG.
OrthoDBiEOG091G0EAQ.
PhylomeDBiQ8WWL2.
TreeFamiTF326239.

Enzyme and pathway databases

BioCyciZFISH:G66-31190-MONOMER.

Miscellaneous databases

ChiTaRSiSPIRE2. human.
GenomeRNAii84501.
PROiQ8WWL2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000204991.
CleanExiHS_SPIRE2.
ExpressionAtlasiQ8WWL2. baseline and differential.
GenevisibleiQ8WWL2. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR011019. KIND_dom.
IPR029901. Spire.
IPR029904. Spire2.
IPR003124. WH2_dom.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR21345. PTHR21345. 2 hits.
PTHR21345:SF5. PTHR21345:SF5. 2 hits.
PfamiPF16474. KIND. 1 hit.
[Graphical view]
SMARTiSM00750. KIND. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS51377. KIND. 1 hit.
PS51082. WH2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPIR2_HUMAN
AccessioniPrimary (citable) accession number: Q8WWL2
Secondary accession number(s): A4QPB1
, Q2TA98, Q6P433, Q8ND47, Q96JJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 18, 2010
Last modified: November 30, 2016
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.