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Protein

Protein spire homolog 2

Gene

SPIRE2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning and asymmetric cell division during meiosis. Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein spire homolog 2
Short name:
Spir-2
Gene namesi
Name:SPIRE2
Synonyms:KIAA1832, SPIR2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:30623. SPIRE2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134926582.

Polymorphism and mutation databases

BioMutaiSPIRE2.
DMDMi296452951.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 714714Protein spire homolog 2PRO_0000320023Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei371 – 3711PhosphoserineCombined sources
Modified residuei442 – 4421PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8WWL2.
MaxQBiQ8WWL2.
PaxDbiQ8WWL2.
PRIDEiQ8WWL2.

PTM databases

iPTMnetiQ8WWL2.
PhosphoSiteiQ8WWL2.

Expressioni

Gene expression databases

BgeeiQ8WWL2.
CleanExiHS_SPIRE2.
ExpressionAtlasiQ8WWL2. baseline and differential.
GenevisibleiQ8WWL2. HS.

Organism-specific databases

HPAiHPA041413.
HPA049415.

Interactioni

Protein-protein interaction databases

BioGridi124102. 7 interactions.
IntActiQ8WWL2. 5 interactions.
STRINGi9606.ENSP00000367494.

Structurei

3D structure databases

ProteinModelPortaliQ8WWL2.
SMRiQ8WWL2. Positions 22-207.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 203182KINDPROSITE-ProRule annotationAdd
BLAST
Domaini248 – 26215WH2 1PROSITE-ProRule annotationAdd
BLAST
Domaini278 – 29619WH2 2PROSITE-ProRule annotationAdd
BLAST
Domaini342 – 35918WH2 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni534 – 55421Spir-boxAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi572 – 59928Cys-richAdd
BLAST

Domaini

Binds to actin monomers via the WH2 domain.By similarity
The Spir-box targets binding to intracellular membrane structures.By similarity

Sequence similaritiesi

Belongs to the spire family.Curated
Contains 1 KIND domain.PROSITE-ProRule annotation
Contains 3 WH2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IK9H. Eukaryota.
ENOG4111E0J. LUCA.
GeneTreeiENSGT00390000003058.
HOGENOMiHOG000013039.
HOVERGENiHBG058898.
InParanoidiQ8WWL2.
KOiK02098.
OMAiCGAADEG.
OrthoDBiEOG77DJ5G.
PhylomeDBiQ8WWL2.
TreeFamiTF326239.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR011019. KIND_dom.
IPR029901. Spire.
IPR029904. Spire2.
IPR003124. WH2_dom.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR21345. PTHR21345. 2 hits.
PTHR21345:SF5. PTHR21345:SF5. 2 hits.
PfamiPF16474. KIND. 1 hit.
[Graphical view]
SMARTiSM00750. KIND. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS51377. KIND. 1 hit.
PS51082. WH2. 3 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WWL2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARAGSCGGA AAGAGRPEPW ELSLEEVLKA YEQPLNEEQA WAVCFQGCRG
60 70 80 90 100
LRGSPGRRLR DTGDLLLRGD GSVGAREPEA AEPATMVVPL ASSEAQTVQS
110 120 130 140 150
LGFAIYRALD WGLDESEERE LSPQLERLID LMANNDSEDS GCGAADEGYG
160 170 180 190 200
GPEEEEEAEG VPRSVRTFAQ AMRLCAARLT DPRGAQAHYQ AVCRALFVET
210 220 230 240 250
LELRAFLARV REAKEMLQKL REDEPHLETP RAELDSLGHT DWARLWVQLM
260 270 280 290 300
RELRRGVKLK KVQEQEFNPL PTEFQLTPFE MLMQDIRARN YKLRKVMVDG
310 320 330 340 350
DIPPRVKKDA HELILDFIRS RPPLKQVSER RLRPLPPKQR SLHEKILEEI
360 370 380 390 400
KQERRLRPVR GEGWAARGFG SLPCILNACS GDAKSTSCIN LSVTDAGGSA
410 420 430 440 450
QRPRPRVLLK APTLAEMEEM NTSEEEESPC GEVTLKRDRS FSEHDLAQLR
460 470 480 490 500
SEVASGLQSA THPPGGTEPP RPRAGSAHVW RPGSRDQGTC PASVSDPSHP
510 520 530 540 550
LLSNRGSSGD RPEASMTPDA KHLWLEFSHP VESLALTVEE VMDVRRVLVK
560 570 580 590 600
AEMEKFLQNK ELFSSLKKGK ICCCCRAKFP LFSWPPSCLF CKRAVCTSCS
610 620 630 640 650
IKMKMPSKKF GHIPVYTLGF ESPQRVSAAK TAPIQRRDIF QSLQGPQWQS
660 670 680 690 700
VEEAFPHIYS HGCVLKDVCS ECTSFVADVV RSSRKSVDVL NTTPRRSRQT
710
QSLYIPNTRT LDFK
Length:714
Mass (Da):79,671
Last modified:May 18, 2010 - v3
Checksum:i0999F9D5DE6A29CD
GO
Isoform 2 (identifier: Q8WWL2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     594-641: Missing.

Show »
Length:666
Mass (Da):74,333
Checksum:i855ED63B0D0D5C1C
GO
Isoform 3 (identifier: Q8WWL2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.
     49-95: RGLRGSPGRR...MVVPLASSEA → MSCLCLGLLW...VPPSDRAPVP

Show »
Length:666
Mass (Da):74,632
Checksum:i6C27E30D5E3B74AC
GO
Isoform 4 (identifier: Q8WWL2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-131: Missing.
     296-297: Missing.

Show »
Length:581
Mass (Da):65,375
Checksum:iC1BD44D6B34746E3
GO

Sequence cautioni

The sequence BAB47461.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAD19439.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti41 – 411W → L in CAD19439 (Ref. 1) Curated
Sequence conflicti216 – 2161M → T in CAD39070 (PubMed:17974005).Curated
Sequence conflicti307 – 3071K → E in BAB47461 (PubMed:11347906).Curated
Sequence conflicti711 – 7111L → F in AAI39733 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 131131Missing in isoform 4. 1 PublicationVSP_031568Add
BLAST
Alternative sequencei1 – 4848Missing in isoform 3. 1 PublicationVSP_031569Add
BLAST
Alternative sequencei49 – 9547RGLRG…ASSEA → MSCLCLGLLWTDSCCLPGRV LGPLHPPAAFSPPHPPAVPP SDRAPVP in isoform 3. 1 PublicationVSP_031570Add
BLAST
Alternative sequencei296 – 2972Missing in isoform 4. 1 PublicationVSP_031571
Alternative sequencei594 – 64148Missing in isoform 2. 1 PublicationVSP_031572Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ422077 mRNA. Translation: CAD19439.1. Different initiation.
AB058735 mRNA. Translation: BAB47461.1. Different initiation.
BC063706 mRNA. Translation: AAH63706.1.
BC111030 mRNA. Translation: AAI11031.1.
BC139732 mRNA. Translation: AAI39733.1.
AL834408 mRNA. Translation: CAD39070.1.
CCDSiCCDS32516.1. [Q8WWL2-1]
RefSeqiNP_115827.1. NM_032451.1. [Q8WWL2-1]
UniGeneiHs.461786.

Genome annotation databases

EnsembliENST00000378247; ENSP00000367494; ENSG00000204991. [Q8WWL2-1]
ENST00000393062; ENSP00000376782; ENSG00000204991. [Q8WWL2-2]
GeneIDi84501.
KEGGihsa:84501.
UCSCiuc002foz.2. human. [Q8WWL2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ422077 mRNA. Translation: CAD19439.1. Different initiation.
AB058735 mRNA. Translation: BAB47461.1. Different initiation.
BC063706 mRNA. Translation: AAH63706.1.
BC111030 mRNA. Translation: AAI11031.1.
BC139732 mRNA. Translation: AAI39733.1.
AL834408 mRNA. Translation: CAD39070.1.
CCDSiCCDS32516.1. [Q8WWL2-1]
RefSeqiNP_115827.1. NM_032451.1. [Q8WWL2-1]
UniGeneiHs.461786.

3D structure databases

ProteinModelPortaliQ8WWL2.
SMRiQ8WWL2. Positions 22-207.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124102. 7 interactions.
IntActiQ8WWL2. 5 interactions.
STRINGi9606.ENSP00000367494.

PTM databases

iPTMnetiQ8WWL2.
PhosphoSiteiQ8WWL2.

Polymorphism and mutation databases

BioMutaiSPIRE2.
DMDMi296452951.

Proteomic databases

EPDiQ8WWL2.
MaxQBiQ8WWL2.
PaxDbiQ8WWL2.
PRIDEiQ8WWL2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000378247; ENSP00000367494; ENSG00000204991. [Q8WWL2-1]
ENST00000393062; ENSP00000376782; ENSG00000204991. [Q8WWL2-2]
GeneIDi84501.
KEGGihsa:84501.
UCSCiuc002foz.2. human. [Q8WWL2-1]

Organism-specific databases

CTDi84501.
GeneCardsiSPIRE2.
H-InvDBHIX0013371.
HGNCiHGNC:30623. SPIRE2.
HPAiHPA041413.
HPA049415.
MIMi609217. gene.
neXtProtiNX_Q8WWL2.
PharmGKBiPA134926582.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IK9H. Eukaryota.
ENOG4111E0J. LUCA.
GeneTreeiENSGT00390000003058.
HOGENOMiHOG000013039.
HOVERGENiHBG058898.
InParanoidiQ8WWL2.
KOiK02098.
OMAiCGAADEG.
OrthoDBiEOG77DJ5G.
PhylomeDBiQ8WWL2.
TreeFamiTF326239.

Miscellaneous databases

ChiTaRSiSPIRE2. human.
GenomeRNAii84501.
PROiQ8WWL2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8WWL2.
CleanExiHS_SPIRE2.
ExpressionAtlasiQ8WWL2. baseline and differential.
GenevisibleiQ8WWL2. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR011019. KIND_dom.
IPR029901. Spire.
IPR029904. Spire2.
IPR003124. WH2_dom.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR21345. PTHR21345. 2 hits.
PTHR21345:SF5. PTHR21345:SF5. 2 hits.
PfamiPF16474. KIND. 1 hit.
[Graphical view]
SMARTiSM00750. KIND. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS51377. KIND. 1 hit.
PS51082. WH2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Drosophila spire gene is highly conserved between species."
    Kerkhoff E., Leberfinger C.B., Borawski J.M., Rapp U.R., Doerks T., Bork P.
    Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O.
    DNA Res. 8:85-95(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
    Tissue: Brain and Skin.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 53-714 (ISOFORM 1).
    Tissue: Brain.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-371 AND SER-442, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Spire-type actin nucleators cooperate with Formin-2 to drive asymmetric oocyte division."
    Pfender S., Kuznetsov V., Pleiser S., Kerkhoff E., Schuh M.
    Curr. Biol. 21:955-960(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSPIR2_HUMAN
AccessioniPrimary (citable) accession number: Q8WWL2
Secondary accession number(s): A4QPB1
, Q2TA98, Q6P433, Q8ND47, Q96JJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 18, 2010
Last modified: June 8, 2016
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.