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Protein

Protein spire homolog 2

Gene

SPIRE2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning and asymmetric cell division during meiosis. Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Protein spire homolog 2
Short name:
Spir-2
Gene namesi
Name:SPIRE2
Synonyms:KIAA1832, SPIR2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000204991.10
HGNCiHGNC:30623 SPIRE2
MIMi609217 gene
neXtProtiNX_Q8WWL2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000204991
PharmGKBiPA134926582

Chemistry databases

DrugBankiDB08080 LATRUNCULIN B

Polymorphism and mutation databases

BioMutaiSPIRE2
DMDMi296452951

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003200231 – 714Protein spire homolog 2Add BLAST714

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei371PhosphoserineCombined sources1
Modified residuei440PhosphoserineCombined sources1
Modified residuei442PhosphoserineCombined sources1
Modified residuei476PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8WWL2
PaxDbiQ8WWL2
PeptideAtlasiQ8WWL2
PRIDEiQ8WWL2
ProteomicsDBi74898
74899 [Q8WWL2-2]
74900 [Q8WWL2-3]
74901 [Q8WWL2-4]

PTM databases

iPTMnetiQ8WWL2
PhosphoSitePlusiQ8WWL2

Expressioni

Gene expression databases

BgeeiENSG00000204991
CleanExiHS_SPIRE2
ExpressionAtlasiQ8WWL2 baseline and differential
GenevisibleiQ8WWL2 HS

Organism-specific databases

HPAiHPA041413
HPA049415

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi124102, 9 interactors
IntActiQ8WWL2, 5 interactors
STRINGi9606.ENSP00000367494

Structurei

Secondary structure

1714
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi414 – 417Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JCYX-ray1.80B401-427[»]
ProteinModelPortaliQ8WWL2
SMRiQ8WWL2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 203KINDPROSITE-ProRule annotationAdd BLAST182
Domaini248 – 262WH2 1PROSITE-ProRule annotationAdd BLAST15
Domaini278 – 296WH2 2PROSITE-ProRule annotationAdd BLAST19
Domaini342 – 359WH2 3PROSITE-ProRule annotationAdd BLAST18

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni534 – 554Spir-boxAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi572 – 599Cys-richAdd BLAST28

Domaini

Binds to actin monomers via the WH2 domain.By similarity
The Spir-box targets binding to intracellular membrane structures.By similarity

Sequence similaritiesi

Belongs to the spire family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IK9H Eukaryota
ENOG4111E0J LUCA
GeneTreeiENSGT00390000003058
HOGENOMiHOG000013039
HOVERGENiHBG058898
InParanoidiQ8WWL2
KOiK02098
OMAiCGAADEG
OrthoDBiEOG091G0EAQ
PhylomeDBiQ8WWL2
TreeFamiTF326239

Family and domain databases

InterProiView protein in InterPro
IPR011019 KIND_dom
IPR029901 Spire
IPR029904 Spire2
IPR003124 WH2_dom
IPR011011 Znf_FYVE_PHD
PANTHERiPTHR21345 PTHR21345, 1 hit
PTHR21345:SF5 PTHR21345:SF5, 1 hit
PfamiView protein in Pfam
PF16474 KIND, 1 hit
SMARTiView protein in SMART
SM00750 KIND, 1 hit
SUPFAMiSSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS51377 KIND, 1 hit
PS51082 WH2, 3 hits

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WWL2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARAGSCGGA AAGAGRPEPW ELSLEEVLKA YEQPLNEEQA WAVCFQGCRG
60 70 80 90 100
LRGSPGRRLR DTGDLLLRGD GSVGAREPEA AEPATMVVPL ASSEAQTVQS
110 120 130 140 150
LGFAIYRALD WGLDESEERE LSPQLERLID LMANNDSEDS GCGAADEGYG
160 170 180 190 200
GPEEEEEAEG VPRSVRTFAQ AMRLCAARLT DPRGAQAHYQ AVCRALFVET
210 220 230 240 250
LELRAFLARV REAKEMLQKL REDEPHLETP RAELDSLGHT DWARLWVQLM
260 270 280 290 300
RELRRGVKLK KVQEQEFNPL PTEFQLTPFE MLMQDIRARN YKLRKVMVDG
310 320 330 340 350
DIPPRVKKDA HELILDFIRS RPPLKQVSER RLRPLPPKQR SLHEKILEEI
360 370 380 390 400
KQERRLRPVR GEGWAARGFG SLPCILNACS GDAKSTSCIN LSVTDAGGSA
410 420 430 440 450
QRPRPRVLLK APTLAEMEEM NTSEEEESPC GEVTLKRDRS FSEHDLAQLR
460 470 480 490 500
SEVASGLQSA THPPGGTEPP RPRAGSAHVW RPGSRDQGTC PASVSDPSHP
510 520 530 540 550
LLSNRGSSGD RPEASMTPDA KHLWLEFSHP VESLALTVEE VMDVRRVLVK
560 570 580 590 600
AEMEKFLQNK ELFSSLKKGK ICCCCRAKFP LFSWPPSCLF CKRAVCTSCS
610 620 630 640 650
IKMKMPSKKF GHIPVYTLGF ESPQRVSAAK TAPIQRRDIF QSLQGPQWQS
660 670 680 690 700
VEEAFPHIYS HGCVLKDVCS ECTSFVADVV RSSRKSVDVL NTTPRRSRQT
710
QSLYIPNTRT LDFK
Length:714
Mass (Da):79,671
Last modified:May 18, 2010 - v3
Checksum:i0999F9D5DE6A29CD
GO
Isoform 2 (identifier: Q8WWL2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     594-641: Missing.

Show »
Length:666
Mass (Da):74,333
Checksum:i855ED63B0D0D5C1C
GO
Isoform 3 (identifier: Q8WWL2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.
     49-95: RGLRGSPGRR...MVVPLASSEA → MSCLCLGLLW...VPPSDRAPVP

Show »
Length:666
Mass (Da):74,632
Checksum:i6C27E30D5E3B74AC
GO
Isoform 4 (identifier: Q8WWL2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-131: Missing.
     296-297: Missing.

Show »
Length:581
Mass (Da):65,375
Checksum:iC1BD44D6B34746E3
GO

Sequence cautioni

The sequence BAB47461 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAD19439 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41W → L in CAD19439 (Ref. 1) Curated1
Sequence conflicti216M → T in CAD39070 (PubMed:17974005).Curated1
Sequence conflicti307K → E in BAB47461 (PubMed:11347906).Curated1
Sequence conflicti711L → F in AAI39733 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0315681 – 131Missing in isoform 4. 1 PublicationAdd BLAST131
Alternative sequenceiVSP_0315691 – 48Missing in isoform 3. 1 PublicationAdd BLAST48
Alternative sequenceiVSP_03157049 – 95RGLRG…ASSEA → MSCLCLGLLWTDSCCLPGRV LGPLHPPAAFSPPHPPAVPP SDRAPVP in isoform 3. 1 PublicationAdd BLAST47
Alternative sequenceiVSP_031571296 – 297Missing in isoform 4. 1 Publication2
Alternative sequenceiVSP_031572594 – 641Missing in isoform 2. 1 PublicationAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ422077 mRNA Translation: CAD19439.1 Different initiation.
AB058735 mRNA Translation: BAB47461.1 Different initiation.
BC063706 mRNA Translation: AAH63706.1
BC111030 mRNA Translation: AAI11031.1
BC139732 mRNA Translation: AAI39733.1
AL834408 mRNA Translation: CAD39070.1
CCDSiCCDS32516.1 [Q8WWL2-1]
RefSeqiNP_115827.1, NM_032451.1 [Q8WWL2-1]
UniGeneiHs.461786

Genome annotation databases

EnsembliENST00000378247; ENSP00000367494; ENSG00000204991 [Q8WWL2-1]
ENST00000393062; ENSP00000376782; ENSG00000204991 [Q8WWL2-2]
GeneIDi84501
KEGGihsa:84501
UCSCiuc002foz.2 human [Q8WWL2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSPIR2_HUMAN
AccessioniPrimary (citable) accession number: Q8WWL2
Secondary accession number(s): A4QPB1
, Q2TA98, Q6P433, Q8ND47, Q96JJ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 18, 2010
Last modified: June 20, 2018
This is version 117 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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