Reviewed,
UniProtKB/Swiss-Prot Q8WWK9 (CKAP2_HUMAN)
Last modified
December 15, 2009.
Version 60.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Cytoskeleton-associated protein 2 Alternative name(s): Tumor- and microtubule-associated protein CTCL tumor antigen se20-10 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 683 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Possesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner By similarity. UniProtKB Q3V1H1 |
| Subunit structure | Associates with alpha- and beta-tubulins By similarity. |
| Subcellular location | Cytoplasm › cytoskeleton. Cytoplasm › cytoskeleton. Cytoplasm › cytoskeleton › spindle. Cytoplasm › cytoskeleton › spindle pole. Note: Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2. At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules. From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle microtubules. During cytokinesis, absent from midbody microtubules. Ref.1 Ref.3 Ref.10 |
| Tissue specificity | Abundant in testis, thymus, and in tumor derived cell lines, while barely detectable in liver, prostate, and kidney. Ref.1 |
| Developmental stage | Present at the G1/S boundary. Accumulates as cells progress from S to G2 into mitosis. Rapidly degraded during mitosis exit by CDH1-activated anaphase promoting complex/cyclosome (APC/C). Ref.10 |
| Induction | Up-regulated in primary human gastric cancers. Ref.3 |
| Sequence similarities | Belongs to the CKAP2 family. |
| Sequence caution | The sequence AAG33675.1 differs from that shown. Reason: Frameshift at position 607. The sequence AAI05807.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Apoptosis Cell cycle |
| Cellular component | Cytoplasm Cytoskeleton Microtubule |
| Coding sequence diversity | Alternative splicing Polymorphism |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | apoptosis Inferred from electronic annotation. Source: UniProtKB-KW cell cycleInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from direct assay. Source: HPA microtubuleInferred from electronic annotation. Source: UniProtKB-KW spindleInferred from electronic annotation. Source: UniProtKB-SubCell |
| Complete GO annotation... | |
Alternative products
| This entry describes 3 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 Ref.1 Ref.3 Ref.8 (identifier: Q8WWK9-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 Ref.6 (identifier: Q8WWK9-2) The sequence of this isoform differs from the canonical sequence as follows: 1-235: Missing. 496-683: Missing. | ||||||
| Note: No experimental confirmation available. | ||||||
| Isoform 3 Ref.3 (identifier: Q8WWK9-3) The sequence of this isoform differs from the canonical sequence as follows: 496-683: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 683 | 683 | Cytoskeleton-associated protein 2 | PRO_0000245774 | |||||
Amino acid modifications | |||||||||
| Modified residue | 69 | 1 | Phosphothreonine Ref.13 | ||||||
| Modified residue | 209 | 1 | Phosphoserine Ref.13 | ||||||
| Modified residue | 211 | 1 | Phosphothreonine Ref.13 | ||||||
| Modified residue | 276 | 1 | Phosphoserine Ref.13 | ||||||
| Modified residue | 280 | 1 | Phosphothreonine Ref.13 | ||||||
| Modified residue | 304 | 1 | Phosphoserine Ref.13 | ||||||
| Modified residue | 305 | 1 | Phosphoserine Ref.13 | ||||||
| Modified residue | 328 | 1 | Phosphoserine Ref.13 | ||||||
| Modified residue | 534 | 1 | Phosphoserine Ref.13 Ref.11 | ||||||
| Modified residue | 579 | 1 | Phosphothreonine Ref.11 Ref.14 | ||||||
| Modified residue | 582 | 1 | Phosphothreonine Ref.11 Ref.14 | ||||||
| Modified residue | 595 | 1 | Phosphoserine Ref.14 | ||||||
| Modified residue | 596 | 1 | Phosphothreonine Ref.14 | ||||||
| Modified residue | 597 | 1 | Phosphothreonine Ref.13 Ref.11 Ref.14 | ||||||
| Modified residue | 599 | 1 | Phosphotyrosine Ref.14 | ||||||
| Modified residue | 602 | 1 | Phosphoserine Ref.11 Ref.14 | ||||||
| Modified residue | 614 | 1 | Phosphoserine Ref.13 Ref.12 | ||||||
| Modified residue | 623 | 1 | Phosphothreonine Ref.11 | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 235 | 235 | Missing in isoform 2. Ref.6 | VSP_052093 | |||||
| Alternative sequence | 496 – 683 | 188 | Missing in isoform 2 and isoform 3. Ref.3 Ref.6 | VSP_052094 | |||||
| Natural variant | 236 | 1 | M → K: dbSNP rs35975899. Ref.8 | VAR_054018 | |||||
| Natural variant | 323 | 1 | I → V: dbSNP rs7335867. Ref.1 Ref.8 Ref.4 Ref.7 Ref.9 | VAR_027005 | |||||
Experimental info | |||||||||
| Sequence conflict | 53 | 1 | Missing in AAL47212. Ref.3 | ||||||
| Sequence conflict | 53 | 1 | Missing in AAL47213. Ref.3 | ||||||
| Sequence conflict | 53 | 1 | Missing in BAA91788. Ref.4 | ||||||
| Sequence conflict | 53 | 1 | Missing in BAB14345. Ref.4 | ||||||
| Sequence conflict | 53 | 1 | Missing in CAH71660. Ref.6 | ||||||
| Sequence conflict | 53 | 1 | Missing in CAH71661. Ref.6 | ||||||
| Sequence conflict | 53 | 1 | Missing in AAI30297. Ref.7 | ||||||
| Sequence conflict | 402 | 1 | P → L in CAB66782. Ref.9 | ||||||
| Sequence conflict | 494 – 495 | 2 | PI → VR in AAL47213. Ref.3 | ||||||
| Sequence conflict | 494 – 495 | 2 | PI → VR in CAH71661. Ref.6 | ||||||
| Sequence conflict | 494 – 495 | 2 | PI → VR in AAH10901. Ref.7 | ||||||
| Sequence conflict | 531 | 1 | K → Q in BAA91788. Ref.4 | ||||||
| Sequence conflict | 531 | 1 | K → R in BAB14345. Ref.4 | ||||||
| Sequence conflict | 559 | 1 | F → L in BAB14345. Ref.4 | ||||||
| Sequence conflict | 577 | 1 | V → A in BAB14345. Ref.4 | ||||||
| Sequence conflict | 643 | 1 | K → R in BAA91788. Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification of a novel cDNA, encoding a cytoskeletal associated protein, differentially expressed in Diffuse Large B-Cell Lymphomas." Maouche-Chretien L., Deleu N., Badoual C., Fraissignes P., Berger R., Gaulard P., Romeo P.H., Leroy-Viard K. Oncogene 17:1245-1251(1998) [PubMed: 9771967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, VARIANT VAL-323. Tissue: T-cell. |
| [2] | "Evolutionarily-conserved gene CKAP2,located in region 13q14.3 of the human genome, is frequently rearranged in various tumors." Udina I.G., Baranova A.V., Kompaniytsev A.A., Sulimova G.E. Genetika 37:120-123(2001) [PubMed: 11234418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1). |
| [3] | "Up-regulation of cytoskeletal-associated protein 2 in primary human gastric adenocarcinomas." Bae C.-D., Sung Y.-S., Jeon S.-M., Suh Y., Yang H.-K., Kim Y.-I., Park K.-H., Choi J., Ahn G., Park J. J. Cancer Res. Clin. Oncol. 129:621-630(2003) [PubMed: 12942315] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), SUBCELLULAR LOCATION, INDUCTION. Tissue: Cervix carcinoma. |
| [4] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-323. Tissue: Teratocarcinoma. |
| [5] | "The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis. |
| [6] | "The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. Ross M.T.Nature 428:522-528(2004) [PubMed: 15057823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [7] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 218-683 (ISOFORM 3), VARIANT VAL-323. Tissue: Bone marrow, Cerebellum and Testis. |
| [8] | "Serological detection of cutaneous T-cell lymphoma-associated antigens." Eichmueller S., Usener D., Dummer R., Stein A., Thiel D., Schadendorf D. Proc. Natl. Acad. Sci. U.S.A. 98:629-634(2001) [PubMed: 11149944] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 38-683 (ISOFORM 1), VARIANTS LYS-236 AND VAL-323. Tissue: Testis. |
| [9] | "Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. Poustka A.Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 135-683 (ISOFORM 1), VARIANT VAL-323. Tissue: Testis. |
| [10] | "CKAP2 is a spindle-associated protein degraded by APC/C-Cdh1 during mitotic exit." Seki A., Fang G. J. Biol. Chem. 282:15103-15113(2007) [PubMed: 17376772] [Abstract] Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE. |
| [11] | "Phosphoproteome analysis of the human mitotic spindle." Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R. Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006) [PubMed: 16565220] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-534; THR-579; THR-582; THR-597; SER-602 AND THR-623, MASS SPECTROMETRY. Tissue: Epithelium. |
| [12] | "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-614, MASS SPECTROMETRY. Tissue: Epithelium. |
| [13] | "Evaluation of the low-specificity protease elastase for large-scale phosphoproteome analysis." Wang B., Malik R., Nigg E.A., Korner R. Anal. Chem. 80:9526-9533(2008) [PubMed: 19007248] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-69; SER-209; THR-211; SER-276; THR-280; SER-304; SER-305; SER-328; SER-534; THR-597 AND SER-614, MASS SPECTROMETRY. |
| [14] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-579; THR-582; SER-595; THR-596; THR-597; TYR-599 AND SER-602, MASS SPECTROMETRY. |
| [15] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-597 AND SER-602, MASS SPECTROMETRY. Tissue: T-cell. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| Y15758 mRNA. Translation: CAC17466.1. AJ429398 Genomic DNA. Translation: CAD22295.1. AY062261 mRNA. Translation: AAL47212.1. AY062262 mRNA. Translation: AAL47213.1. AK001611 mRNA. Translation: BAA91788.1. Different initiation. AK022982 mRNA. Translation: BAB14345.1. EF560732 mRNA. Translation: ABQ59042.1. AL359513 Genomic DNA. Translation: CAH71660.2. AL359513 Genomic DNA. Translation: CAH71661.2. BC010901 mRNA. Translation: AAH10901.1. Different initiation. BC105806 mRNA. Translation: AAI05807.1. Sequence problems. BC130296 mRNA. Translation: AAI30297.1. AF177227 mRNA. Translation: AAG33675.1. Frameshift. AL136848 mRNA. Translation: CAB66782.2. | |
| IPI | IPI00071824. IPI00640794. IPI00946815. |
| RefSeq | NP_001091995.1. NP_060674.3. |
| UniGene | Hs.444028 Hs.449585 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q8WWK9. |
PTM databases | |
| PhosphoSite | Q8WWK9. |
Proteomic databases | |
| PRIDE | Q8WWK9. |
Genome annotation databases | |
| Ensembl | ENST00000378037; ENSP00000367276; ENSG00000136108; Homo sapiens. [Genome view] |
| GeneID | 26586. |
| KEGG | hsa:26586. |
| UCSC | uc001vgt.2. human. uc001vgv.2. human. |
Organism-specific databases | |
| CTD | 26586. |
| GeneCards | GC13P051927. |
| HGNC | HGNC:1990. CKAP2. |
| HPA | HPA008410. |
| MIM | 611569. gene. |
| PharmGKB | PA26526. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOVERGEN | Q8WWK9. |
| InParanoid | Q8WWK9. |
| OMA | CLIKYNV. |
| OrthoDB | EOG9QC3QW. |
Gene expression databases | |
| ArrayExpress | Q8WWK9. |
| Bgee | Q8WWK9. |
| Genevestigator | Q8WWK9. |
| GermOnline | ENSG00000136108. Homo sapiens. |
Family and domain databases | |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 48956. |
| SOURCE | Search... |
Entry information
| Entry name | CKAP2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q8WWK9 Secondary accession number(s): A2BDE0 Q9NVG4 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 13 Human chromosome 13: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


