Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable tRNA pseudouridine synthase 1

Gene

TRUB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May be responsible for synthesis of pseudouridine from uracil in transfer RNAs.By similarity

Catalytic activityi

tRNA uridine = tRNA pseudouridine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei121 – 1211NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA pseudouridine synthase 1 (EC:5.4.99.-)
Gene namesi
Name:TRUB1
Synonyms:PUS4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:16060. TRUB1.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38086.

Polymorphism and mutation databases

BioMutaiTRUB1.
DMDMi74751577.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 349348Probable tRNA pseudouridine synthase 1PRO_0000252087Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Modified residuei11 – 111PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8WWH5.
MaxQBiQ8WWH5.
PaxDbiQ8WWH5.
PeptideAtlasiQ8WWH5.
PRIDEiQ8WWH5.
TopDownProteomicsiQ8WWH5.

PTM databases

iPTMnetiQ8WWH5.
PhosphoSiteiQ8WWH5.

Expressioni

Tissue specificityi

Highly expressed in heart, skeletal muscle and liver. Expressed at lower levels in lung, small intestine, kidney and spleen.1 Publication

Gene expression databases

BgeeiQ8WWH5.
CleanExiHS_TRUB1.
GenevisibleiQ8WWH5. HS.

Organism-specific databases

HPAiHPA037914.
HPA057552.

Interactioni

Protein-protein interaction databases

BioGridi126783. 9 interactions.
IntActiQ8WWH5. 4 interactions.
MINTiMINT-3047472.
STRINGi9606.ENSP00000298746.

Structurei

3D structure databases

ProteinModelPortaliQ8WWH5.
SMRiQ8WWH5. Positions 70-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family.Curated

Phylogenomic databases

eggNOGiKOG2529. Eukaryota.
COG0130. LUCA.
GeneTreeiENSGT00530000063611.
HOGENOMiHOG000063679.
HOVERGENiHBG094094.
InParanoidiQ8WWH5.
KOiK03177.
OMAiKFQPPFF.
OrthoDBiEOG7RFTHX.
PhylomeDBiQ8WWH5.
TreeFamiTF320759.

Family and domain databases

HAMAPiMF_01080. TruB_bact.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8WWH5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASEAAVVS SPSLKTDTSP VLETAGTVAA MAATPSARAA AAVVAAAART
60 70 80 90 100
GSEARVSKAA LATKLLSLSG VFAVHKPKGP TSAELLNRLK EKLLAEAGMP
110 120 130 140 150
SPEWTKRKKQ TLKIGHGGTL DSAARGVLVV GIGSGTKMLT SMLSGSKRYT
160 170 180 190 200
AIGELGKATD TLDSTGRVTE EKPYDKITQE DIEGILQKFT GNIMQVPPLY
210 220 230 240 250
SALKKDGQRL STLMKRGEVV EAKPARPVTV YSISLQKFQP PFFTLDVECG
260 270 280 290 300
GGFYIRSLVS DIGKELSSCA NVLELTRTKQ GPFTLEEHAL PEDKWTIDDI
310 320 330 340
AQSLEHCSSL FPAELALKKS KPESNEQVLS CEYITLNEPK REDDVIKTC
Length:349
Mass (Da):37,253
Last modified:March 1, 2002 - v1
Checksum:iC3292D31EC60EE17
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti323 – 3231E → D in BAD97287 (Ref. 4) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti103 – 1031E → A.
Corresponds to variant rs34393297 [ dbSNP | Ensembl ].
VAR_051607
Natural varianti167 – 1671R → K.1 Publication
Corresponds to variant rs7099565 [ dbSNP | Ensembl ].
VAR_027748

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448144 mRNA. Translation: AAL40350.1.
BX648709 mRNA. Translation: CAH10560.1.
AK312805 mRNA. Translation: BAG35663.1.
AK223567 mRNA. Translation: BAD97287.1.
AL355340 Genomic DNA. Translation: CAH73219.1.
BC030601 mRNA. Translation: AAH30601.1.
CCDSiCCDS7591.1.
RefSeqiNP_631908.1. NM_139169.4.
UniGeneiHs.21187.

Genome annotation databases

EnsembliENST00000298746; ENSP00000298746; ENSG00000165832.
GeneIDi142940.
KEGGihsa:142940.
UCSCiuc001lcd.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448144 mRNA. Translation: AAL40350.1.
BX648709 mRNA. Translation: CAH10560.1.
AK312805 mRNA. Translation: BAG35663.1.
AK223567 mRNA. Translation: BAD97287.1.
AL355340 Genomic DNA. Translation: CAH73219.1.
BC030601 mRNA. Translation: AAH30601.1.
CCDSiCCDS7591.1.
RefSeqiNP_631908.1. NM_139169.4.
UniGeneiHs.21187.

3D structure databases

ProteinModelPortaliQ8WWH5.
SMRiQ8WWH5. Positions 70-343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126783. 9 interactions.
IntActiQ8WWH5. 4 interactions.
MINTiMINT-3047472.
STRINGi9606.ENSP00000298746.

PTM databases

iPTMnetiQ8WWH5.
PhosphoSiteiQ8WWH5.

Polymorphism and mutation databases

BioMutaiTRUB1.
DMDMi74751577.

Proteomic databases

EPDiQ8WWH5.
MaxQBiQ8WWH5.
PaxDbiQ8WWH5.
PeptideAtlasiQ8WWH5.
PRIDEiQ8WWH5.
TopDownProteomicsiQ8WWH5.

Protocols and materials databases

DNASUi142940.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000298746; ENSP00000298746; ENSG00000165832.
GeneIDi142940.
KEGGihsa:142940.
UCSCiuc001lcd.4. human.

Organism-specific databases

CTDi142940.
GeneCardsiTRUB1.
HGNCiHGNC:16060. TRUB1.
HPAiHPA037914.
HPA057552.
MIMi610726. gene.
neXtProtiNX_Q8WWH5.
PharmGKBiPA38086.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2529. Eukaryota.
COG0130. LUCA.
GeneTreeiENSGT00530000063611.
HOGENOMiHOG000063679.
HOVERGENiHBG094094.
InParanoidiQ8WWH5.
KOiK03177.
OMAiKFQPPFF.
OrthoDBiEOG7RFTHX.
PhylomeDBiQ8WWH5.
TreeFamiTF320759.

Miscellaneous databases

ChiTaRSiTRUB1. human.
GenomeRNAii142940.
NextBioi84645.
PROiQ8WWH5.
SOURCEiSearch...

Gene expression databases

BgeeiQ8WWH5.
CleanExiHS_TRUB1.
GenevisibleiQ8WWH5. HS.

Family and domain databases

HAMAPiMF_01080. TruB_bact.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The human TruB family of pseudouridine synthase genes, including the Dyskeratosis Congenita 1 gene and the novel member TRUB1."
    Zucchini C., Strippoli P., Biolchi A., Solmi R., Lenzi L., D'Addabbo P., Carinci P., Valvassori L.
    Int. J. Mol. Med. 11:697-704(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Foreskin.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Endometrial tumor.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LYS-167.
    Tissue: Testis.
  4. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.
  5. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTRUB1_HUMAN
AccessioniPrimary (citable) accession number: Q8WWH5
Secondary accession number(s): B2R716, Q53ES2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: March 1, 2002
Last modified: May 11, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.