Q8WWA0 (ITLN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Intelectin-1
Short name=ITLN-1

Alternative name(s):
Endothelial lectin HL-1
Galactofuranose-binding lectin
Intestinal lactoferrin receptor
Omentin

Gene names

Name:	ITLN1
Synonyms:	INTL, ITLN, LFR
ORF Names:	UNQ640/PRO1270

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. Increases AKT phosphorylation in the absence and presence of insulin. May play a role in the defense system against microorganisms. May specifically recognize carbohydrate chains of pathogens and bacterial components containing galactofuranosyl residues, in a calcium-dependent manner. May be involved in iron metabolism. Ref.3 Ref.5
Subunit structure	Homotrimer; disulfide-linked. Ref.3 Ref.13
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor. Secreted. Note: Enriched in lipid rafts By similarity. Ref.1 Ref.3 Ref.5
Tissue specificity	Highly expressed in omental adipose tissue where it is found in stromal vascular cells but not in fat cells but is barely detectable in subcutaneous adipose tissue (at protein level). Highly expressed in the small intestine. Also found in the heart, testis, colon, salivary gland, skeletal muscle, pancreas and thyroid and, to a lesser degree, in the uterus, spleen, prostate, lymph node and thymus. Ref.1 Ref.2 Ref.3 Ref.5
Developmental stage	Found in fetal small intestine and thymus.
Post-translational modification	N-glycosylated. Ref.3 Ref.13
Sequence similarities	Contains 1 fibrinogen C-terminal domain.
Mass spectrometry	Molecular mass is 35500 Da from positions 19 - 298. Determined by MALDI. Ref.13

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Ligand	Lectin
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of glucose import Inferred from direct assay Ref.5. Source: UniProtKB positive regulation of protein phosphorylation Inferred from direct assay Ref.5. Source: UniProtKB response to nematode Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW brush border membrane Inferred from sequence or structural similarity. Source: UniProtKB extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell membrane raft Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.3
	Chain	19 – 298	280	Intelectin-1		PRO_0000009143
	Propeptide	299 – 313	15	Potential		PRO_0000009144
Regions
	Domain	32 – 255	224	Fibrinogen C-terminal
Amino acid modifications
	Lipidation	298	1	GPI-anchor amidated serine Potential
	Glycosylation	163	1	N-linked (GlcNAc...) Probable
	Disulfide bond	31 ↔ 48		Ref.13
Natural variations
	Natural variant	109	1	V → D. Ref.3 Corresponds to variant rs2274907 [ dbSNP | Ensembl ].		VAR_019924
	Natural variant	313	1	R → P. Ref.4 Ref.6 Corresponds to variant rs8144 [ dbSNP | Ensembl ].		VAR_019925
Experimental info
	Mutagenesis	31	1	C → S: Forms mainly monomers; when associated with S-48. Ref.13
	Mutagenesis	48	1	C → S: Forms mainly dimers. Forms mainly monomers; when associated with S-31. Ref.13

Sequences

	Sequence		Length	Mass (Da)
	Q8WWA0 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 56219FE937FC802E	FASTA	313	34,962
	10 20 30 40 50 60 MNQLSFLLFL IATTRGWSTD EANTYFKEWT CSSSPSLPRS CKEIKDECPS AFDGLYFLRT 70 80 90 100 110 120 ENGVIYQTFC DMTSGGGGWT LVASVHENDM RGKCTVGDRW SSQQGSKAVY PEGDGNWANY 130 140 150 160 170 180 NTFGSAEAAT SDDYKNPGYY DIQAKDLGIW HVPNKSPMQH WRNSSLLRYR TDTGFLQTLG 190 200 210 220 230 240 HNLFGIYQKY PVKYGEGKCW TDNGPVIPVV YDFGDAQKTA SYYSPYGQRE FTAGFVQFRV 250 260 270 280 290 300 FNNERAANAL CAGMRVTGCN TEHHCIGGGG YFPEASPQQC GDFSGFDWSG YGTHVGYSSS 310 REITEAAVLL FYR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and functional expression of a human intestinal lactoferrin receptor." Suzuki Y.A., Shin K., Loennerdal B. Biochemistry 40:15771-15779(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GPI-ANCHOR, TISSUE SPECIFICITY. Tissue: Small intestine.
[2]	"Human homologs of the Xenopus oocyte cortical granule lectin XL35." Lee J.K., Schnee J., Pang M., Wolfert M., Baum L.G., Moremen K.W., Pierce M. Glycobiology 11:65-73(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Small intestine.
[3]	"Human intelectin is a novel soluble lectin that recognizes galactofuranose in carbohydrate chains of bacterial cell wall." Tsuji S., Uehori J., Matsumoto M., Suzuki Y., Matsuhisa A., Toyoshima K., Seya T. J. Biol. Chem. 276:23456-23463(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-28, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, SUBUNIT, GLYCOSYLATION, VARIANT ASP-109. Tissue: Placenta.
[4]	"The Xenopus laevis cortical granule lectin: cDNA cloning, developmental expression, and identification of the eglectin family of lectins." Chang B.Y., Peavy T.R., Wardrip N.J., Hedrick J.L. Comp. Biochem. Physiol. 137A:115-129(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT PRO-313.
[5]	"Identification of omentin as a novel depot-specific adipokine in human adipose tissue: possible role in modulating insulin action." Yang R.-Z., Lee M.-J., Hu H., Pray J., Wu H.-B., Hansen B.C., Shuldiner A.R., Fried S.K., McLenithan J.C., Gong D.-W. Am. J. Physiol. 290:E1253-E1261(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Adipose tissue.
[6]	"Human homolog of the Xenopus laevis egg cortical granule lectin." Peavy T.R., Hedrick J.L. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT PRO-313. Tissue: Ovary.
[7]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Adipose tissue.
[9]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[10]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[11]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[12]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[13]	"Differential structure and activity between human and mouse intelectin-1: human intelectin-1 is a disulfide-linked trimer, whereas mouse homologue is a monomer." Tsuji S., Yamashita M., Nishiyama A., Shinohara T., Li Z., Myrvik Q.N., Hoffman D.R., Henriksen R.A., Shibata Y. Glycobiology 17:1045-1051(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, MASS SPECTROMETRY, GLYCOSYLATION, DISULFIDE BOND, MUTAGENESIS OF CYS-31 AND CYS-48.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF271386 mRNA. Translation: AAM20741.1. AY065972 mRNA. Translation: AAL58073.1. AB036706 mRNA. Translation: BAA96094.1. AY157361 mRNA. Translation: AAO17800.1. AY157362 mRNA. Translation: AAO17801.1. AY549722 mRNA. Translation: AAS49907.1. AY619692 mRNA. Translation: AAU88048.1. AY358359 mRNA. Translation: AAQ88725.1. AK000029 mRNA. Translation: BAA90893.1. CR457224 mRNA. Translation: CAG33505.1. AL354714 Genomic DNA. Translation: CAH72357.1. CH471121 Genomic DNA. Translation: EAW52690.1. BC020664 mRNA. Translation: AAH20664.1.
IPI	IPI00291737.
RefSeq	NP_060095.2. NM_017625.2.
UniGene	Hs.50813.
3D structure databases
ProteinModelPortal	Q8WWA0.
SMR	Q8WWA0. Positions 38-127.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000323587.
PTM databases
PhosphoSite	Q8WWA0.
Polymorphism databases
DMDM	55976553.
Proteomic databases
PaxDb	Q8WWA0.
PRIDE	Q8WWA0.
Protocols and materials databases
DNASU	55600.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000326245; ENSP00000323587; ENSG00000179914.
GeneID	55600.
KEGG	hsa:55600.
UCSC	uc001fxc.3. human.
Organism-specific databases
CTD	55600.
GeneCards	GC01M160846.
HGNC	HGNC:18259. ITLN1.
HPA	CAB012652.
MIM	609873. gene.
neXtProt	NX_Q8WWA0.
PharmGKB	PA134870726.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG25076.
HOGENOM	HOG000026788.
HOVERGEN	HBG052157.
InParanoid	Q8WWA0.
OMA	KEIKDEC.
OrthoDB	EOG4NGGN0.
PhylomeDB	Q8WWA0.
Gene expression databases
Bgee	Q8WWA0.
CleanEx	HS_ITLN1.
Genevestigator	Q8WWA0.
GermOnline	ENSG00000179914. Homo sapiens.
Family and domain databases
Gene3D	3.90.215.10. 1 hit.
InterPro	IPR014716. Fibrinogen_a/b/g_C_1. IPR002181. Fibrinogen_a/b/g_C_dom. [Graphical view]
SMART	SM00186. FBG. 1 hit. [Graphical view]
SUPFAM	SSF56496. Fibrinogen_a/b/g_C. 1 hit.
PROSITE	PS00514. FIBRINOGEN_C_1. False negative. PS51406. FIBRINOGEN_C_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ITLN1. human.
GenomeRNAi	55600.
NextBio	60138.
SOURCE	Search...

Entry information

Entry name

ITLN1_HUMAN

Accession

Primary (citable) accession number: Q8WWA0
Secondary accession number(s): Q5IWS4 , Q5VYI4, Q6YDJ3, Q9NP67

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.