ID   HMGB4_HUMAN             Reviewed;         186 AA.
AC   Q8WW32; B2R4X7; Q0QWA4;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2012, sequence version 2.
DT   24-JAN-2024, entry version 150.
DE   RecName: Full=High mobility group protein B4;
GN   Name=HMGB4;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT ALA-92.
RC   TISSUE=Testis;
RA   Zhou C., Xiao X.W., Zhou J.L., Zhang J.;
RT   "Identification of a novel H. sapiens protein with two high-mobility group
RT   domains.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16710414; DOI=10.1038/nature04727;
RA   Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA   Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA   Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA   Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA   Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA   Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA   Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA   Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA   Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA   Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA   Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA   Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA   Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA   Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA   Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA   Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA   Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA   Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA   Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA   McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA   Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA   Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA   Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA   Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA   Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA   White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA   Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA   Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA   Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT   "The DNA sequence and biological annotation of human chromosome 1.";
RL   Nature 441:315-321(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ALA-92.
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- INTERACTION:
CC       Q8WW32; Q8NHQ1: CEP70; NbExp=6; IntAct=EBI-10277297, EBI-739624;
CC       Q8WW32; P60329: KRTAP12-4; NbExp=3; IntAct=EBI-10277297, EBI-10176396;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q6P8W9}.
CC       Chromosome {ECO:0000250|UniProtKB:Q6P8W9}. Note=Interacts specifically
CC       with the sex chromosomes. {ECO:0000250|UniProtKB:Q6P8W9}.
CC   -!- SIMILARITY: Belongs to the HMGB family. {ECO:0000305}.
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DR   EMBL; DQ207367; ABB22048.1; -; mRNA.
DR   EMBL; AK311985; BAG34924.1; -; mRNA.
DR   EMBL; AL121980; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC021180; AAH21180.1; -; mRNA.
DR   EMBL; CH471059; EAX07453.1; -; Genomic_DNA.
DR   CCDS; CCDS30668.1; -.
DR   RefSeq; NP_660206.2; NM_145205.5.
DR   AlphaFoldDB; Q8WW32; -.
DR   SMR; Q8WW32; -.
DR   BioGRID; 126064; 4.
DR   IntAct; Q8WW32; 3.
DR   STRING; 9606.ENSP00000430919; -.
DR   iPTMnet; Q8WW32; -.
DR   PhosphoSitePlus; Q8WW32; -.
DR   BioMuta; HMGB4; -.
DR   DMDM; 387912835; -.
DR   EPD; Q8WW32; -.
DR   MassIVE; Q8WW32; -.
DR   PaxDb; 9606-ENSP00000430919; -.
DR   PeptideAtlas; Q8WW32; -.
DR   ProteomicsDB; 74853; -.
DR   Antibodypedia; 17205; 284 antibodies from 28 providers.
DR   DNASU; 127540; -.
DR   Ensembl; ENST00000519684.5; ENSP00000429214.1; ENSG00000176256.11.
DR   Ensembl; ENST00000522796.1; ENSP00000430919.1; ENSG00000176256.11.
DR   Ensembl; ENST00000681531.1; ENSP00000505691.1; ENSG00000176256.11.
DR   GeneID; 127540; -.
DR   KEGG; hsa:127540; -.
DR   MANE-Select; ENST00000681531.1; ENSP00000505691.1; NM_001379301.1; NP_001366230.1.
DR   UCSC; uc001bxp.3; human.
DR   AGR; HGNC:24954; -.
DR   CTD; 127540; -.
DR   DisGeNET; 127540; -.
DR   GeneCards; HMGB4; -.
DR   HGNC; HGNC:24954; HMGB4.
DR   HPA; ENSG00000176256; Tissue enriched (testis).
DR   neXtProt; NX_Q8WW32; -.
DR   OpenTargets; ENSG00000176256; -.
DR   VEuPathDB; HostDB:ENSG00000176256; -.
DR   eggNOG; KOG0381; Eukaryota.
DR   GeneTree; ENSGT00940000162735; -.
DR   HOGENOM; CLU_082854_0_4_1; -.
DR   InParanoid; Q8WW32; -.
DR   OMA; MWSAKTD; -.
DR   OrthoDB; 1222485at2759; -.
DR   PhylomeDB; Q8WW32; -.
DR   TreeFam; TF105371; -.
DR   PathwayCommons; Q8WW32; -.
DR   SignaLink; Q8WW32; -.
DR   BioGRID-ORCS; 127540; 10 hits in 1146 CRISPR screens.
DR   GeneWiki; HMGB4; -.
DR   GenomeRNAi; 127540; -.
DR   Pharos; Q8WW32; Tbio.
DR   PRO; PR:Q8WW32; -.
DR   Proteomes; UP000005640; Chromosome 1.
DR   RNAct; Q8WW32; Protein.
DR   Bgee; ENSG00000176256; Expressed in right testis and 107 other cell types or tissues.
DR   GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; HDA:UniProtKB.
DR   GO; GO:0008301; F:DNA binding, bending; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd21978; HMG-box_HMGB_rpt1; 1.
DR   CDD; cd21979; HMG-box_HMGB_rpt2; 1.
DR   Gene3D; 1.10.30.10; High mobility group box domain; 2.
DR   InterPro; IPR009071; HMG_box_dom.
DR   InterPro; IPR036910; HMG_box_dom_sf.
DR   PANTHER; PTHR48112:SF7; HIGH MOBILITY GROUP PROTEIN B4; 1.
DR   PANTHER; PTHR48112; HIGH MOBILITY GROUP PROTEIN DSP1; 1.
DR   Pfam; PF00505; HMG_box; 1.
DR   Pfam; PF09011; HMG_box_2; 1.
DR   PRINTS; PR00886; HIGHMOBLTY12.
DR   SMART; SM00398; HMG; 2.
DR   SUPFAM; SSF47095; HMG-box; 2.
DR   PROSITE; PS50118; HMG_BOX_2; 2.
DR   Genevisible; Q8WW32; HS.
PE   1: Evidence at protein level;
KW   Chromosome; DNA-binding; Nucleus; Reference proteome; Repeat.
FT   CHAIN           1..186
FT                   /note="High mobility group protein B4"
FT                   /id="PRO_0000269180"
FT   DNA_BIND        9..79
FT                   /note="HMG box 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT   DNA_BIND        93..161
FT                   /note="HMG box 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT   REGION          77..98
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         59
FT                   /note="K -> R (in dbSNP:rs11542686)"
FT                   /id="VAR_055951"
FT   VARIANT         92
FT                   /note="E -> A (in dbSNP:rs10379)"
FT                   /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.1"
FT                   /id="VAR_067467"
FT   VARIANT         170
FT                   /note="Y -> H (in dbSNP:rs57068937)"
FT                   /id="VAR_067468"
FT   CONFLICT        33
FT                   /note="N -> S (in Ref. 5; AAH21180)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   186 AA;  22490 MW;  E7F941ABBC8D3025 CRC64;
     MGKEIQLKPK ANVSSYVHFL LNYRNKFKEQ QPNTYVGFKE FSRKCSEKWR SISKHEKAKY
     EALAKLDKAR YQEEMMNYVG KRKKRRKRDP QEPRRPPSSF LLFCQDHYAQ LKRENPNWSV
     VQVAKATGKM WSTATDLEKH PYEQRVALLR AKYFEELELY RKQCNARKKY RMSARNRCRG
     KRVRQS
//