ID   ABEC4_HUMAN             Reviewed;         367 AA.
AC   Q8WW27; Q8N7F6;
DT   13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   27-MAR-2024, entry version 141.
DE   RecName: Full=Putative C->U-editing enzyme APOBEC-4;
DE            EC=3.5.4.-;
DE   AltName: Full=Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 4;
GN   Name=APOBEC4; Synonyms=C1orf169;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS SER-271 AND GLU-331.
RC   TISSUE=Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16710414; DOI=10.1038/nature04727;
RA   Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA   Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA   Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA   Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA   Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA   Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA   Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA   Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA   Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA   Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA   Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA   Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA   Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA   Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA   Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA   Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA   Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA   Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA   Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA   McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA   Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA   Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA   Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA   Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA   Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA   White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA   Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA   Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA   Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT   "The DNA sequence and biological annotation of human chromosome 1.";
RL   Nature 441:315-321(2006).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   IDENTIFICATION, AND TISSUE SPECIFICITY.
RX   PubMed=16082223; DOI=10.4161/cc.4.9.1994;
RA   Rogozin I.B., Basu M.K., Jordan I.K., Pavlov Y.I., Koonin E.V.;
RT   "APOBEC4, a new member of the AID/APOBEC family of polynucleotide
RT   (deoxy)cytidine deaminases predicted by computational analysis.";
RL   Cell Cycle 4:1281-1285(2005).
CC   -!- FUNCTION: Putative C to U editing enzyme whose physiological substrate
CC       is not yet known. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC   -!- INTERACTION:
CC       Q8WW27; P55212: CASP6; NbExp=3; IntAct=EBI-25836284, EBI-718729;
CC       Q8WW27; P62826: RAN; NbExp=3; IntAct=EBI-25836284, EBI-286642;
CC   -!- TISSUE SPECIFICITY: Predominantly expressed in testis.
CC       {ECO:0000269|PubMed:16082223}.
CC   -!- SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase
CC       family. {ECO:0000305}.
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DR   EMBL; AK098557; BAC05332.1; -; mRNA.
DR   EMBL; AL137800; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC021711; AAH21711.1; -; mRNA.
DR   CCDS; CCDS1358.1; -.
DR   RefSeq; NP_982279.1; NM_203454.2.
DR   AlphaFoldDB; Q8WW27; -.
DR   SMR; Q8WW27; -.
DR   BioGRID; 135625; 3.
DR   IntAct; Q8WW27; 2.
DR   STRING; 9606.ENSP00000310622; -.
DR   iPTMnet; Q8WW27; -.
DR   PhosphoSitePlus; Q8WW27; -.
DR   BioMuta; APOBEC4; -.
DR   DMDM; 74751569; -.
DR   MassIVE; Q8WW27; -.
DR   PaxDb; 9606-ENSP00000310622; -.
DR   PeptideAtlas; Q8WW27; -.
DR   ProteomicsDB; 74852; -.
DR   Antibodypedia; 2817; 295 antibodies from 28 providers.
DR   DNASU; 403314; -.
DR   Ensembl; ENST00000308641.6; ENSP00000310622.4; ENSG00000173627.8.
DR   GeneID; 403314; -.
DR   KEGG; hsa:403314; -.
DR   MANE-Select; ENST00000308641.6; ENSP00000310622.4; NM_203454.3; NP_982279.1.
DR   UCSC; uc001gqn.4; human.
DR   AGR; HGNC:32152; -.
DR   CTD; 403314; -.
DR   GeneCards; APOBEC4; -.
DR   HGNC; HGNC:32152; APOBEC4.
DR   HPA; ENSG00000173627; Group enriched (fallopian tube, testis).
DR   MIM; 609908; gene.
DR   neXtProt; NX_Q8WW27; -.
DR   OpenTargets; ENSG00000173627; -.
DR   PharmGKB; PA142672597; -.
DR   VEuPathDB; HostDB:ENSG00000173627; -.
DR   eggNOG; ENOG502QQXT; Eukaryota.
DR   GeneTree; ENSGT00390000014243; -.
DR   HOGENOM; CLU_832944_0_0_1; -.
DR   InParanoid; Q8WW27; -.
DR   OMA; HNAYEIN; -.
DR   OrthoDB; 5349603at2759; -.
DR   PhylomeDB; Q8WW27; -.
DR   TreeFam; TF338173; -.
DR   PathwayCommons; Q8WW27; -.
DR   Reactome; R-HSA-72200; mRNA Editing: C to U Conversion.
DR   Reactome; R-HSA-75094; Formation of the Editosome.
DR   SignaLink; Q8WW27; -.
DR   BioGRID-ORCS; 403314; 29 hits in 1139 CRISPR screens.
DR   ChiTaRS; APOBEC4; human.
DR   GeneWiki; APOBEC4; -.
DR   GenomeRNAi; 403314; -.
DR   Pharos; Q8WW27; Tdark.
DR   PRO; PR:Q8WW27; -.
DR   Proteomes; UP000005640; Chromosome 1.
DR   RNAct; Q8WW27; Protein.
DR   Bgee; ENSG00000173627; Expressed in bronchial epithelial cell and 39 other cell types or tissues.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.140.10; Cytidine Deaminase, domain 2; 1.
DR   InterPro; IPR038953; APOBEC4.
DR   InterPro; IPR002125; CMP_dCMP_dom.
DR   PANTHER; PTHR35672; C-U-EDITING ENZYME APOBEC-4-RELATED; 1.
DR   PANTHER; PTHR35672:SF1; C-U-EDITING ENZYME APOBEC-4-RELATED; 1.
DR   Pfam; PF18778; NAD1; 1.
DR   PROSITE; PS51747; CYT_DCMP_DEAMINASES_2; 1.
DR   Genevisible; Q8WW27; HS.
PE   1: Evidence at protein level;
KW   Hydrolase; Metal-binding; mRNA processing; Reference proteome; Zinc.
FT   CHAIN           1..367
FT                   /note="Putative C->U-editing enzyme APOBEC-4"
FT                   /id="PRO_0000239355"
FT   DOMAIN          61..177
FT                   /note="CMP/dCMP-type deaminase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01083"
FT   ACT_SITE        95
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         93
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         127
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         134
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   VARIANT         75
FT                   /note="S -> F (in dbSNP:rs16861394)"
FT                   /id="VAR_026639"
FT   VARIANT         271
FT                   /note="F -> S (in dbSNP:rs1174658)"
FT                   /evidence="ECO:0000269|PubMed:14702039"
FT                   /id="VAR_026640"
FT   VARIANT         275
FT                   /note="P -> S (in dbSNP:rs10911391)"
FT                   /id="VAR_026641"
FT   VARIANT         300
FT                   /note="D -> G (in dbSNP:rs16861381)"
FT                   /id="VAR_048724"
FT   VARIANT         331
FT                   /note="K -> E (in dbSNP:rs1174657)"
FT                   /evidence="ECO:0000269|PubMed:14702039"
FT                   /id="VAR_026642"
FT   VARIANT         345
FT                   /note="V -> M (in dbSNP:rs10911390)"
FT                   /id="VAR_026643"
SQ   SEQUENCE   367 AA;  41581 MW;  B5ACD9AB875482C6 CRC64;
     MEPIYEEYLA NHGTIVKPYY WLSFSLDCSN CPYHIRTGEE ARVSLTEFCQ IFGFPYGTTF
     PQTKHLTFYE LKTSSGSLVQ KGHASSCTGN YIHPESMLFE MNGYLDSAIY NNDSIRHIIL
     YSNNSPCNEA NHCCISKMYN FLITYPGITL SIYFSQLYHT EMDFPASAWN REALRSLASL
     WPRVVLSPIS GGIWHSVLHS FISGVSGSHV FQPILTGRAL ADRHNAYEIN AITGVKPYFT
     DVLLQTKRNP NTKAQEALES YPLNNAFPGQ FFQMPSGQLQ PNLPPDLRAP VVFVLVPLRD
     LPPMHMGQNP NKPRNIVRHL NMPQMSFQET KDLGRLPTGR SVEIVEITEQ FASSKEADEK
     KKKKGKK
//