Q8WW22 (DNJA4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DnaJ homolog subfamily A member 4

Gene names

Name:

DNAJA4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Lipid-anchor Potential.
Sequence similarities	Contains 1 CR-type zinc finger. Contains 1 J domain.
Sequence caution	The sequence AAH21720.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAH31044.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Chaperone
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro response to heat Inferred from electronic annotation. Source: InterPro
Cellular_component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8WW22-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8WW22-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MARGGSQSWSSGESDGQPKEQTPEKPRHKM

Isoform 3 (identifier: Q8WW22-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: MVKETQYYDILGVKPSASPEEIKKAYRKLALKYHPDKNPDEGEK → MWESLTLDSGQISALTR

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	DnaJ homolog subfamily A member 4		PRO_0000071014
	Propeptide	395 – 397	3	Removed in mature form By similarity		PRO_0000396760
Regions
	Domain	4 – 70	67	J
	Repeat	135 – 142	8	CXXCXGXG motif
	Repeat	151 – 158	8	CXXCXGXG motif
	Repeat	178 – 185	8	CXXCXGXG motif
	Repeat	194 – 201	8	CXXCXGXG motif
	Zinc finger	122 – 206	85	CR-type
	Compositional bias	75 – 96	22	Gly-rich
Sites
	Metal binding	135	1	Zinc 1 By similarity
	Metal binding	138	1	Zinc 1 By similarity
	Metal binding	151	1	Zinc 2 By similarity
	Metal binding	154	1	Zinc 2 By similarity
	Metal binding	178	1	Zinc 2 By similarity
	Metal binding	181	1	Zinc 2 By similarity
	Metal binding	194	1	Zinc 1 By similarity
	Metal binding	197	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	394	1	Cysteine methyl ester By similarity
	Lipidation	394	1	S-farnesyl cysteine By similarity
Natural variations
	Alternative sequence	1 – 44	44	MVKET…DEGEK → MWESLTLDSGQISALTR in isoform 3.		VSP_045696
	Alternative sequence	1	1	M → MARGGSQSWSSGESDGQPKE QTPEKPRHKM in isoform 2.		VSP_038965
	Natural variant	226	1	H → R. Ref.5 Corresponds to variant rs17852881 [ dbSNP | Ensembl ].		VAR_069064
Experimental info
	Sequence conflict	33	1	Y → C in BAC05229. Ref.1
Isoform 3:
	Sequence conflict	1	1	M → T in AAH31044. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 60D6AAD12C9B2529	FASTA	397	44,798
	10 20 30 40 50 60 MVKETQYYDI LGVKPSASPE EIKKAYRKLA LKYHPDKNPD EGEKFKLISQ AYEVLSDPKK 70 80 90 100 110 120 RDVYDQGGEQ AIKEGGSGSP SFSSPMDIFD MFFGGGGRMA RERRGKNVVH QLSVTLEDLY 130 140 150 160 170 180 NGVTKKLALQ KNVICEKCEG VGGKKGSVEK CPLCKGRGMQ IHIQQIGPGM VQQIQTVCIE 190 200 210 220 230 240 CKGQGERINP KDRCESCSGA KVIREKKIIE VHVEKGMKDG QKILFHGEGD QEPELEPGDV 250 260 270 280 290 300 IIVLDQKDHS VFQRRGHDLI MKMKIQLSEA LCGFKKTIKT LDNRILVITS KAGEVIKHGD 310 320 330 340 350 360 LRCVRDEGMP IYKAPLEKGI LIIQFLVIFP EKHWLSLEKL PQLEALLPPR QKVRITDDMD 370 380 390 QVELKEFCPN EQNWRQHREA YEEDEDGPQA GVQCQTA
	Isoform 2 [UniParc]. Checksum: 820C0BE7F54EB221	FASTA	426	47,963
	10 20 30 40 50 60 MARGGSQSWS SGESDGQPKE QTPEKPRHKM VKETQYYDIL GVKPSASPEE IKKAYRKLAL 70 80 90 100 110 120 KYHPDKNPDE GEKFKLISQA YEVLSDPKKR DVYDQGGEQA IKEGGSGSPS FSSPMDIFDM 130 140 150 160 170 180 FFGGGGRMAR ERRGKNVVHQ LSVTLEDLYN GVTKKLALQK NVICEKCEGV GGKKGSVEKC 190 200 210 220 230 240 PLCKGRGMQI HIQQIGPGMV QQIQTVCIEC KGQGERINPK DRCESCSGAK VIREKKIIEV 250 260 270 280 290 300 HVEKGMKDGQ KILFHGEGDQ EPELEPGDVI IVLDQKDHSV FQRRGHDLIM KMKIQLSEAL 310 320 330 340 350 360 CGFKKTIKTL DNRILVITSK AGEVIKHGDL RCVRDEGMPI YKAPLEKGIL IIQFLVIFPE 370 380 390 400 410 420 KHWLSLEKLP QLEALLPPRQ KVRITDDMDQ VELKEFCPNE QNWRQHREAY EEDEDGPQAG VQCQTA
	Isoform 3 [UniParc]. Checksum: 3FCEABA5F96D48A2	FASTA	370	41,597
	10 20 30 40 50 60 MWESLTLDSG QISALTRFKL ISQAYEVLSD PKKRDVYDQG GEQAIKEGGS GSPSFSSPMD 70 80 90 100 110 120 IFDMFFGGGG RMARERRGKN VVHQLSVTLE DLYNGVTKKL ALQKNVICEK CEGVGGKKGS 130 140 150 160 170 180 VEKCPLCKGR GMQIHIQQIG PGMVQQIQTV CIECKGQGER INPKDRCESC SGAKVIREKK 190 200 210 220 230 240 IIEVHVEKGM KDGQKILFHG EGDQEPELEP GDVIIVLDQK DHSVFQRRGH DLIMKMKIQL 250 260 270 280 290 300 SEALCGFKKT IKTLDNRILV ITSKAGEVIK HGDLRCVRDE GMPIYKAPLE KGILIIQFLV 310 320 330 340 350 360 IFPEKHWLSL EKLPQLEALL PPRQKVRITD DMDQVELKEF CPNEQNWRQH REAYEEDEDG 370 PQAGVQCQTA

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Trachea.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Endometrial tumor.
[3]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT ARG-226. Tissue: Brain and Testis.
[6]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK096616 mRNA. Translation: BAC04828.1. AK098079 mRNA. Translation: BAC05229.1. BX648710 mRNA. Translation: CAH10558.1. AC092755 Genomic DNA. No translation available. CH471136 Genomic DNA. Translation: EAW99177.1. BC021720 mRNA. Translation: AAH21720.1. Different initiation. BC031044 mRNA. Translation: AAH31044.2. Different initiation.
IPI	IPI00465105. IPI00794825. IPI00853174.
RefSeq	NP_001123654.1. NM_001130182.1. NP_001123655.1. NM_001130183.1. NP_061072.3. NM_018602.3.
UniGene	Hs.513053.
3D structure databases
ProteinModelPortal	Q8WW22.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8WW22. 3 interactions.
STRING	9606.ENSP00000378324.
PTM databases
PhosphoSite	Q8WW22.
Polymorphism databases
DMDM	27805462.
Proteomic databases
PaxDb	Q8WW22.
PRIDE	Q8WW22.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000343789; ENSP00000339581; ENSG00000140403. ENST00000394852; ENSP00000378321; ENSG00000140403. ENST00000394855; ENSP00000378324; ENSG00000140403. ENST00000446172; ENSP00000413499; ENSG00000140403.
GeneID	55466.
KEGG	hsa:55466.
UCSC	uc002bdi.3. human. uc002bdj.2. human.
Organism-specific databases
CTD	55466.
GeneCards	GC15P078556.
HGNC	HGNC:14885. DNAJA4.
HPA	CAB004646.
neXtProt	NX_Q8WW22.
PharmGKB	PA27411.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0484.
HOGENOM	HOG000226718.
HOVERGEN	HBG066727.
InParanoid	Q8WW22.
KO	K09505.
OMA	PEKHWLS.
OrthoDB	EOG4RJG1R.
PhylomeDB	Q8WW22.
Gene expression databases
ArrayExpress	Q8WW22.
Bgee	Q8WW22.
CleanEx	HS_DNAJA4.
Genevestigator	Q8WW22.
GermOnline	ENSG00000140403. Homo sapiens.
Family and domain databases
Gene3D	1.10.287.110. 1 hit. 2.10.230.10. 1 hit.
InterPro	IPR012724. DnaJ. IPR002939. DnaJ_C. IPR001623. DnaJ_domain. IPR018253. DnaJ_domain_CS. IPR008971. HSP40/DnaJ_pept-bd. IPR001305. HSP_DnaJ_Cys-rich_dom. [Graphical view]
Pfam	PF00226. DnaJ. 1 hit. PF01556. DnaJ_C. 1 hit. PF00684. DnaJ_CXXCXGXG. 1 hit. [Graphical view]
PRINTS	PR00625. JDOMAIN.
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
SUPFAM	SSF46565. DnaJ_N. 1 hit. SSF49493. HSP40_DnaJ_pep. 2 hits. SSF57938. HSP_DnaJ_cys-rich. 1 hit.
PROSITE	PS00636. DNAJ_1. 1 hit. PS50076. DNAJ_2. 1 hit. PS51188. ZF_CR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	DNAJA4. human.
GenomeRNAi	55466.
NextBio	59854.

Entry information

Entry name

DNJA4_HUMAN

Accession

Primary (citable) accession number: Q8WW22
Secondary accession number(s): E9PDM9 , Q6AW87, Q8N5Z4, Q8N7P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.