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Q8WW12

- PCNP_HUMAN

UniProt

Q8WW12 - PCNP_HUMAN

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Protein

PEST proteolytic signal-containing nuclear protein

Gene

PCNP

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be involved in cell cycle regulation.

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. proteasome-mediated ubiquitin-dependent protein catabolic process Source: HGNC
  3. protein ubiquitination Source: HGNC
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Names & Taxonomyi

Protein namesi
Recommended name:
PEST proteolytic signal-containing nuclear protein
Short name:
PCNP
Short name:
PEST-containing nuclear protein
Gene namesi
Name:PCNP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:30023. PCNP.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA143485572.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 178177PEST proteolytic signal-containing nuclear proteinPRO_0000058253Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei64 – 641N6-acetyllysine1 Publication
Modified residuei87 – 871Phosphoserine1 Publication
Modified residuei119 – 1191Phosphoserine2 Publications
Modified residuei139 – 1391Phosphothreonine1 Publication
Modified residuei147 – 1471Phosphoserine1 Publication
Modified residuei150 – 1501N6-acetyllysine1 Publication
Modified residuei152 – 1521N6-acetyllysine1 Publication

Post-translational modificationi

Ubiquitinated; mediated by UHRF2 and leading to its subsequent proteasomal degradation.1 Publication
N-terminally acetylated in a HYPK-dependent manner by the NatA acetyltransferase complex which is composed of NAA10 and NAA15.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ8WW12.
PaxDbiQ8WW12.
PRIDEiQ8WW12.

PTM databases

PhosphoSiteiQ8WW12.

Expressioni

Gene expression databases

BgeeiQ8WW12.
CleanExiHS_PCNP.
ExpressionAtlasiQ8WW12. baseline and differential.
GenevestigatoriQ8WW12.

Organism-specific databases

HPAiHPA035011.

Interactioni

Subunit structurei

Interacts with UHRF2/NIRF.2 Publications

Protein-protein interaction databases

BioGridi121360. 7 interactions.
STRINGi9606.ENSP00000265260.

Structurei

3D structure databases

ProteinModelPortaliQ8WW12.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG46363.
GeneTreeiENSGT00390000010218.
HOVERGENiHBG059581.
InParanoidiQ8WW12.
OMAiPAKMSKF.
OrthoDBiEOG7J70HR.
PhylomeDBiQ8WW12.
TreeFamiTF333058.

Family and domain databases

InterProiIPR029169. PCNP.
[Graphical view]
PANTHERiPTHR16523. PTHR16523. 1 hit.
PfamiPF15473. PCNP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8WW12-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADGKAGDEK PEKSQRAGAA GGPEEEAEKP VKTKTVSSSN GGESSSRSAE
60 70 80 90 100
KRSAEEEAAD LPTKPTKISK FGFAIGSQTT KKASAISIKL GSSKPKETVP
110 120 130 140 150
TLAPKTLSVA AAFNEDEDSE PEEMPPEAKM RMKNIGRDTP TSAGPNSFNK
160 170
GKHGFSDNQK LWERNIKSHL GNVHDQDN
Length:178
Mass (Da):18,925
Last modified:June 7, 2005 - v2
Checksum:iB79D49B362892AE1
GO
Isoform 2 (identifier: Q8WW12-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     119-130: SEPEEMPPEAKM → GYTNISWTKLLQ
     131-178: Missing.

Note: No experimental confirmation available.

Show »
Length:130
Mass (Da):13,544
Checksum:i60256023D3435196
GO
Isoform 3 (identifier: Q8WW12-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.

Show »
Length:55
Mass (Da):6,233
Checksum:iC95818E33C1461F0
GO

Sequence cautioni

The sequence EAW79790.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence EAW79792.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti126 – 1261P → Q in AAH22001. (PubMed:15489334)Curated
Sequence conflicti150 – 1501K → E in BAD96698. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 123123Missing in isoform 3. 1 PublicationVSP_013879Add
BLAST
Alternative sequencei119 – 13012SEPEE…PEAKM → GYTNISWTKLLQ in isoform 2. 1 PublicationVSP_013880Add
BLAST
Alternative sequencei131 – 17848Missing in isoform 2. 1 PublicationVSP_013881Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB037675 mRNA. Translation: BAB03501.1.
AK314629 mRNA. Translation: BAG37194.1.
AK222978 mRNA. Translation: BAD96698.1.
CR627371 mRNA. Translation: CAH10470.1.
CH471052 Genomic DNA. Translation: EAW79790.1. Different initiation.
CH471052 Genomic DNA. Translation: EAW79792.1. Different initiation.
BC013916 mRNA. Translation: AAH13916.1.
BC022001 mRNA. Translation: AAH22001.1.
CCDSiCCDS2942.1. [Q8WW12-1]
RefSeqiNP_065090.1. NM_020357.1. [Q8WW12-1]
UniGeneiHs.744968.

Genome annotation databases

EnsembliENST00000265260; ENSP00000265260; ENSG00000081154. [Q8WW12-1]
ENST00000469941; ENSP00000470810; ENSG00000081154. [Q8WW12-3]
GeneIDi57092.
KEGGihsa:57092.
UCSCiuc003dva.3. human. [Q8WW12-1]
uc003dvd.3. human. [Q8WW12-2]

Polymorphism databases

DMDMi67460963.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB037675 mRNA. Translation: BAB03501.1 .
AK314629 mRNA. Translation: BAG37194.1 .
AK222978 mRNA. Translation: BAD96698.1 .
CR627371 mRNA. Translation: CAH10470.1 .
CH471052 Genomic DNA. Translation: EAW79790.1 . Different initiation.
CH471052 Genomic DNA. Translation: EAW79792.1 . Different initiation.
BC013916 mRNA. Translation: AAH13916.1 .
BC022001 mRNA. Translation: AAH22001.1 .
CCDSi CCDS2942.1. [Q8WW12-1 ]
RefSeqi NP_065090.1. NM_020357.1. [Q8WW12-1 ]
UniGenei Hs.744968.

3D structure databases

ProteinModelPortali Q8WW12.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121360. 7 interactions.
STRINGi 9606.ENSP00000265260.

PTM databases

PhosphoSitei Q8WW12.

Polymorphism databases

DMDMi 67460963.

Proteomic databases

MaxQBi Q8WW12.
PaxDbi Q8WW12.
PRIDEi Q8WW12.

Protocols and materials databases

DNASUi 57092.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000265260 ; ENSP00000265260 ; ENSG00000081154 . [Q8WW12-1 ]
ENST00000469941 ; ENSP00000470810 ; ENSG00000081154 . [Q8WW12-3 ]
GeneIDi 57092.
KEGGi hsa:57092.
UCSCi uc003dva.3. human. [Q8WW12-1 ]
uc003dvd.3. human. [Q8WW12-2 ]

Organism-specific databases

CTDi 57092.
GeneCardsi GC03P101292.
HGNCi HGNC:30023. PCNP.
HPAi HPA035011.
MIMi 615210. gene.
neXtProti NX_Q8WW12.
PharmGKBi PA143485572.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG46363.
GeneTreei ENSGT00390000010218.
HOVERGENi HBG059581.
InParanoidi Q8WW12.
OMAi PAKMSKF.
OrthoDBi EOG7J70HR.
PhylomeDBi Q8WW12.
TreeFami TF333058.

Miscellaneous databases

ChiTaRSi PCNP. human.
GenomeRNAii 57092.
NextBioi 35489760.
PROi Q8WW12.
SOURCEi Search...

Gene expression databases

Bgeei Q8WW12.
CleanExi HS_PCNP.
ExpressionAtlasi Q8WW12. baseline and differential.
Genevestigatori Q8WW12.

Family and domain databases

InterProi IPR029169. PCNP.
[Graphical view ]
PANTHERi PTHR16523. PTHR16523. 1 hit.
Pfami PF15473. PCNP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "NIRF, a novel RING finger protein, is involved in cell-cycle regulation."
    Mori T., Li Y., Hata H., Ono K., Kochi H.
    Biochem. Biophys. Res. Commun. 296:530-536(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, INTERACTION WITH UHRF2.
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Small intestine.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Brain cortex.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain and Testis.
  7. "NIRF is a ubiquitin ligase that is capable of ubiquitinating PCNP, a PEST-containing nuclear protein."
    Mori T., Li Y., Hata H., Kochi H.
    FEBS Lett. 557:209-214(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION, INTERACTION WITH UHRF2.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87; SER-119 AND THR-139, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-64; LYS-150 AND LYS-152, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "The chaperone-like protein HYPK acts together with NatA in cotranslational N-terminal acetylation and prevention of Huntingtin aggregation."
    Arnesen T., Starheim K.K., Van Damme P., Evjenth R., Dinh H., Betts M.J., Ryningen A., Vandekerckhove J., Gevaert K., Anderson D.
    Mol. Cell. Biol. 30:1898-1909(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION AT ALA-2 BY NATA ACETYLTRANSFERASE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-119 AND SER-147, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPCNP_HUMAN
AccessioniPrimary (citable) accession number: Q8WW12
Secondary accession number(s): B2RBE7
, D3DN52, Q53GF3, Q6AI44, Q96CU3, Q9NS81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: October 29, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3