Q8WW12 (PCNP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PEST proteolytic signal-containing nuclear protein
Short name=PCNP
Short name=PEST-containing nuclear protein

Gene names

Name:

PCNP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in cell cycle regulation.
Subunit structure	Interacts with UHRF2/NIRF. Ref.1 Ref.7
Subcellular location	Nucleus Ref.1.
Post-translational modification	Ubiquitinated; mediated by UHRF2 and leading to its subsequent proteasomal degradation. Ref.7 N-terminally acetylated in a HYPK-dependent manner by the NatA acetyltransferase complex which is composed of NAA10 and NAA15.
Sequence caution	The sequence EAW79790.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence EAW79792.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
PTM	Acetylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW proteasomal ubiquitin-dependent protein catabolic process Inferred from direct assay Ref.7. Source: HGNC protein ubiquitination Inferred from direct assay Ref.7. Source: HGNC
Cellular_component	nucleus Inferred from direct assay Ref.1. Source: HGNC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8WW12-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8WW12-2)

The sequence of this isoform differs from the canonical sequence as follows:
     119-130: SEPEEMPPEAKM → GYTNISWTKLLQ
     131-178: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8WW12-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 178	177	PEST proteolytic signal-containing nuclear protein		PRO_0000058253
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.10
	Modified residue	64	1	N6-acetyllysine Ref.9
	Modified residue	87	1	Phosphoserine Ref.8
	Modified residue	119	1	Phosphoserine Ref.8 Ref.12
	Modified residue	139	1	Phosphothreonine Ref.8
	Modified residue	147	1	Phosphoserine Ref.12
	Modified residue	150	1	N6-acetyllysine Ref.9
	Modified residue	152	1	N6-acetyllysine Ref.9
Natural variations
	Alternative sequence	1 – 123	123	Missing in isoform 3.		VSP_013879
	Alternative sequence	119 – 130	12	SEPEE…PEAKM → GYTNISWTKLLQ in isoform 2.		VSP_013880
	Alternative sequence	131 – 178	48	Missing in isoform 2.		VSP_013881
Experimental info
	Sequence conflict	126	1	P → Q in AAH22001. Ref.6
	Sequence conflict	150	1	K → E in BAD96698. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 7, 2005. Version 2. Checksum: B79D49B362892AE1	FASTA	178	18,925
	10 20 30 40 50 60 MADGKAGDEK PEKSQRAGAA GGPEEEAEKP VKTKTVSSSN GGESSSRSAE KRSAEEEAAD 70 80 90 100 110 120 LPTKPTKISK FGFAIGSQTT KKASAISIKL GSSKPKETVP TLAPKTLSVA AAFNEDEDSE 130 140 150 160 170 PEEMPPEAKM RMKNIGRDTP TSAGPNSFNK GKHGFSDNQK LWERNIKSHL GNVHDQDN
	Isoform 2 [UniParc]. Checksum: 60256023D3435196	FASTA	130	13,544
	10 20 30 40 50 60 MADGKAGDEK PEKSQRAGAA GGPEEEAEKP VKTKTVSSSN GGESSSRSAE KRSAEEEAAD 70 80 90 100 110 120 LPTKPTKISK FGFAIGSQTT KKASAISIKL GSSKPKETVP TLAPKTLSVA AAFNEDEDGY 130 TNISWTKLLQ
	Isoform 3 [UniParc]. Checksum: C95818E33C1461F0	FASTA	55	6,233
	10 20 30 40 50 MPPEAKMRMK NIGRDTPTSA GPNSFNKGKH GFSDNQKLWE RNIKSHLGNV HDQDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NIRF, a novel RING finger protein, is involved in cell-cycle regulation." Mori T., Li Y., Hata H., Ono K., Kochi H. Biochem. Biophys. Res. Commun. 296:530-536(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, INTERACTION WITH UHRF2. Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Small intestine.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain cortex.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Testis.
[7]	"NIRF is a ubiquitin ligase that is capable of ubiquitinating PCNP, a PEST-containing nuclear protein." Mori T., Li Y., Hata H., Kochi H. FEBS Lett. 557:209-214(2004) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION, INTERACTION WITH UHRF2.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87; SER-119 AND THR-139, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-64; LYS-150 AND LYS-152, MASS SPECTROMETRY.
[10]	"The chaperone-like protein HYPK acts together with NatA in cotranslational N-terminal acetylation and prevention of Huntingtin aggregation." Arnesen T., Starheim K.K., Van Damme P., Evjenth R., Dinh H., Betts M.J., Ryningen A., Vandekerckhove J., Gevaert K., Anderson D. Mol. Cell. Biol. 30:1898-1909(2010) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION AT ALA-2 BY NATA ACETYLTRANSFERASE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-119 AND SER-147, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB037675 mRNA. Translation: BAB03501.1. AK314629 mRNA. Translation: BAG37194.1. AK222978 mRNA. Translation: BAD96698.1. CR627371 mRNA. Translation: CAH10470.1. CH471052 Genomic DNA. Translation: EAW79790.1. Different initiation. CH471052 Genomic DNA. Translation: EAW79792.1. Different initiation. BC013916 mRNA. Translation: AAH13916.1. BC022001 mRNA. Translation: AAH22001.1.
IPI	IPI00060650. IPI00604582. IPI00790305.
RefSeq	NP_065090.1. NM_020357.1.
UniGene	Hs.275865.
3D structure databases
ProteinModelPortal	Q8WW12.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000265260.
PTM databases
PhosphoSite	Q8WW12.
Polymorphism databases
DMDM	67460963.
Proteomic databases
PaxDb	Q8WW12.
PRIDE	Q8WW12.
Protocols and materials databases
DNASU	57092.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000265260; ENSP00000265260; ENSG00000081154. ENST00000296024; ENSP00000296024; ENSG00000081154. ENST00000469941; ENSP00000470810; ENSG00000081154.
GeneID	57092.
KEGG	hsa:57092.
UCSC	uc003dva.3. human. uc003dvd.3. human.
Organism-specific databases
CTD	57092.
GeneCards	GC03P101292.
HGNC	HGNC:30023. PCNP.
HPA	HPA035011.
neXtProt	NX_Q8WW12.
PharmGKB	PA143485572.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG46363.
HOVERGEN	HBG059581.
InParanoid	Q8WW12.
OMA	PAKMSKF.
OrthoDB	EOG4CNQSN.
PhylomeDB	Q8WW12.
Gene expression databases
ArrayExpress	Q8WW12.
Bgee	Q8WW12.
CleanEx	HS_PCNP.
Genevestigator	Q8WW12.
Family and domain databases
ProtoNet	Search...
Other
ChiTaRS	PCNP. human.
GenomeRNAi	57092.
NextBio	62899.

Entry information

Entry name

PCNP_HUMAN

Accession

Primary (citable) accession number: Q8WW12
Secondary accession number(s): B2RBE7 , D3DN52, Q53GF3, Q6AI44, Q96CU3, Q9NS81

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	May 29, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.