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Protein

Trafficking protein particle complex subunit 12

Gene

TRAPPC12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in chromosome congression, kinetochore assembly and stability and controls the recruitment of CENPE to the kinetochores (PubMed:25918224). May be involved in endoplasmic reticulum to Golgi apparatus trafficking at a very early stage (PubMed:21525244).2 Publications

GO - Molecular functioni

GO - Biological processi

  • metaphase plate congression Source: UniProtKB
  • negative regulation of mitotic spindle assembly checkpoint Source: UniProtKB
  • positive regulation of protein localization to kinetochore Source: UniProtKB
  • regulation of kinetochore assembly Source: UniProtKB
  • vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000171853-MONOMER.
ReactomeiR-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
Trafficking protein particle complex subunit 12
Alternative name(s):
Tetratricopeptide repeat protein 15
Short name:
TPR repeat protein 15
Short name:
TTC-15
Trafficking of membranes and mitosis1 Publication
Gene namesi
Name:TRAPPC12
Synonyms:TRAMM1 Publication, TTC15
ORF Names:CGI-87
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:24284. TRAPPC12.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
  • kinetochore Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi51112.
OpenTargetsiENSG00000171853.
PharmGKBiPA134944710.

Polymorphism and mutation databases

BioMutaiTRAPPC12.
DMDMi116242834.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001064011 – 735Trafficking protein particle complex subunit 12Add BLAST735

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei109PhosphoserineBy similarity1
Modified residuei184PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated as the cells enter mitosis but is dephosphorylated at or before the onset of anaphase. The phosphorylated form recruits CENPE to kinetochores more efficiently than the non-phosphorylated form.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8WVT3.
MaxQBiQ8WVT3.
PaxDbiQ8WVT3.
PeptideAtlasiQ8WVT3.
PRIDEiQ8WVT3.

PTM databases

iPTMnetiQ8WVT3.
PhosphoSitePlusiQ8WVT3.

Expressioni

Gene expression databases

BgeeiENSG00000171853.
CleanExiHS_TTC15.
ExpressionAtlasiQ8WVT3. baseline and differential.
GenevisibleiQ8WVT3. HS.

Organism-specific databases

HPAiHPA034799.

Interactioni

Subunit structurei

Component of the multisubunit TRAPP (transport protein particle) complex, which includes at least TRAPPC2, TRAPPC2L, TRAPPC3, TRAPPC3L, TRAPPC4, TRAPPC5, TRAPPC8, TRAPPC9, TRAPPC10, TRAPPC11 and TRAPPC12 (PubMed:21525244). Interacts with CENPE (PubMed:25918224).2 Publications

Protein-protein interaction databases

BioGridi119301. 25 interactors.
DIPiDIP-48283N.
IntActiQ8WVT3. 6 interactors.
STRINGi9606.ENSP00000324318.

Structurei

3D structure databases

ProteinModelPortaliQ8WVT3.
SMRiQ8WVT3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati545 – 578TPR 1Add BLAST34
Repeati580 – 613TPR 2Add BLAST34
Repeati620 – 653TPR 3Add BLAST34
Repeati654 – 687TPR 4Add BLAST34

Sequence similaritiesi

Contains 4 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG2796. Eukaryota.
ENOG410XSX3. LUCA.
GeneTreeiENSGT00390000002448.
HOGENOMiHOG000154703.
HOVERGENiHBG068968.
InParanoidiQ8WVT3.
KOiK20309.
OMAiEPFAHIQ.
OrthoDBiEOG091G0BUP.
PhylomeDBiQ8WVT3.
TreeFamiTF320881.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
SMARTiSM00028. TPR. 4 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50005. TPR. 4 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8WVT3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDAGGGEET PAPEAPHPPQ LAPPEEQGLL FQEETIDLGG DEFGSEENET
60 70 80 90 100
ASEGSSPLAD KLNEHMMESV LISDSPNSEG DAGDLGRVRD EAEPGGEGDP
110 120 130 140 150
GPEPAGTPSP SGEADGDCAP EDAAPSSGGA PRQDAAREVP GSEAARPEQE
160 170 180 190 200
PPVAEPVPVC TIFSQRAPPA SGDGFEPQMV KSPSFGGASE ASARTPPQVV
210 220 230 240 250
QPSPSLSTFF GDTAASHSLA SDFFDSFTTS AFISVSNPGA GSPAPASPPP
260 270 280 290 300
LAVPGTEGRP EPVAMRGPQA AAPPASPEPF AHIQAVFAGS DDPFATALSM
310 320 330 340 350
SEMDRRNDAW LPGEATRGVL RAVATQQRGA VFVDKENLTM PGLRFDNIQG
360 370 380 390 400
DAVKDLMLRF LGEKAAAKRQ VLNADSVEQS FVGLKQLISC RNWRAAVDLC
410 420 430 440 450
GRLLTAHGQG YGKSGLLTSH TTDSLQLWFV RLALLVKLGL FQNAEMEFEP
460 470 480 490 500
FGNLDQPDLY YEYYPHVYPG RRGSMVPFSM RILHAELQQY LGNPQESLDR
510 520 530 540 550
LHKVKTVCSK ILANLEQGLA EDGGMSSVTQ EGRQASIRLW RSRLGRVMYS
560 570 580 590 600
MANCLLLMKD YVLAVEAYHS VIKYYPEQEP QLLSGIGRIS LQIGDIKTAE
610 620 630 640 650
KYFQDVEKVT QKLDGLQGKI MVLMNSAFLH LGQNNFAEAH RFFTEILRMD
660 670 680 690 700
PRNAVANNNA AVCLLYLGKL KDSLRQLEAM VQQDPRHYLH ESVLFNLTTM
710 720 730
YELESSRSMQ KKQALLEAVA GKEGDSFNTQ CLKLA
Length:735
Mass (Da):79,375
Last modified:October 17, 2006 - v3
Checksum:iB844FB3749866E3F
GO

Sequence cautioni

The sequence AAD34082 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti451F → V in AAD34082 (PubMed:10810093).Curated1
Sequence conflicti486E → D in AAD34082 (PubMed:10810093).Curated1
Sequence conflicti566E → D in AAH14164 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_028442301S → G.1 PublicationCorresponds to variant rs11686212dbSNPEnsembl.1
Natural variantiVAR_035869717E → Q in a breast cancer sample; somatic mutation. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK098327 mRNA. Translation: BAG53613.1.
CH471053 Genomic DNA. Translation: EAX01067.1.
CH471053 Genomic DNA. Translation: EAX01068.1.
BC014164 mRNA. Translation: AAH14164.2.
BC017475 mRNA. Translation: AAH17475.2.
AF151845 mRNA. Translation: AAD34082.1. Different initiation.
CCDSiCCDS1652.1.
RefSeqiNP_001308031.1. NM_001321102.1.
NP_057114.5. NM_016030.5.
UniGeneiHs.252713.
Hs.661278.

Genome annotation databases

EnsembliENST00000324266; ENSP00000324318; ENSG00000171853.
ENST00000382110; ENSP00000371544; ENSG00000171853.
GeneIDi51112.
KEGGihsa:51112.
UCSCiuc002qxm.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK098327 mRNA. Translation: BAG53613.1.
CH471053 Genomic DNA. Translation: EAX01067.1.
CH471053 Genomic DNA. Translation: EAX01068.1.
BC014164 mRNA. Translation: AAH14164.2.
BC017475 mRNA. Translation: AAH17475.2.
AF151845 mRNA. Translation: AAD34082.1. Different initiation.
CCDSiCCDS1652.1.
RefSeqiNP_001308031.1. NM_001321102.1.
NP_057114.5. NM_016030.5.
UniGeneiHs.252713.
Hs.661278.

3D structure databases

ProteinModelPortaliQ8WVT3.
SMRiQ8WVT3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119301. 25 interactors.
DIPiDIP-48283N.
IntActiQ8WVT3. 6 interactors.
STRINGi9606.ENSP00000324318.

PTM databases

iPTMnetiQ8WVT3.
PhosphoSitePlusiQ8WVT3.

Polymorphism and mutation databases

BioMutaiTRAPPC12.
DMDMi116242834.

Proteomic databases

EPDiQ8WVT3.
MaxQBiQ8WVT3.
PaxDbiQ8WVT3.
PeptideAtlasiQ8WVT3.
PRIDEiQ8WVT3.

Protocols and materials databases

DNASUi51112.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000324266; ENSP00000324318; ENSG00000171853.
ENST00000382110; ENSP00000371544; ENSG00000171853.
GeneIDi51112.
KEGGihsa:51112.
UCSCiuc002qxm.2. human.

Organism-specific databases

CTDi51112.
DisGeNETi51112.
GeneCardsiTRAPPC12.
HGNCiHGNC:24284. TRAPPC12.
HPAiHPA034799.
MIMi614139. gene.
neXtProtiNX_Q8WVT3.
OpenTargetsiENSG00000171853.
PharmGKBiPA134944710.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2796. Eukaryota.
ENOG410XSX3. LUCA.
GeneTreeiENSGT00390000002448.
HOGENOMiHOG000154703.
HOVERGENiHBG068968.
InParanoidiQ8WVT3.
KOiK20309.
OMAiEPFAHIQ.
OrthoDBiEOG091G0BUP.
PhylomeDBiQ8WVT3.
TreeFamiTF320881.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000171853-MONOMER.
ReactomeiR-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

ChiTaRSiTRAPPC12. human.
GenomeRNAii51112.
PROiQ8WVT3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000171853.
CleanExiHS_TTC15.
ExpressionAtlasiQ8WVT3. baseline and differential.
GenevisibleiQ8WVT3. HS.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
SMARTiSM00028. TPR. 4 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50005. TPR. 4 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPC12_HUMAN
AccessioniPrimary (citable) accession number: Q8WVT3
Secondary accession number(s): B3KV01
, D6W4Y2, Q8WVW1, Q9Y395
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: October 17, 2006
Last modified: November 30, 2016
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.