Ubiquitin-conjugating enzyme E2 Q2 - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot Q8WVN8 (UB2Q2_HUMAN)

Last modified June 16, 2009. Version 65. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 Q2
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase Q2
    Ubiquitin carrier protein Q2

Gene names

Name:

UBE2Q2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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Function	Catalyzes the covalent attachment of ubiquitin to other proteins By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Cytoplasm. Ref.3
Tissue specificity	Detected in hypopharyngeal head and neck squamous cell carcinoma, in tumor masses and invasive epithelium. Ref.3
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

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Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 375

375

Ubiquitin-conjugating enzyme E2 Q2

PRO_0000223879

Compositional bias

134 – 178

Glu-rich

Active site

304

Glycyl thioester intermediate By similarity

Modified residue

369

Phosphothreonine Ref.4

Alternative sequence

296 – 314

YVLGG…KQGWS → LVHPSKGRWLNMLTVVCLD in isoform 2.

VSP_017298

Alternative sequence

315 – 375

Missing in isoform 2.

VSP_017299

375

Helix Strand Turn

Details...

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Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 7DE07315E89178A3 Show »	FASTA	375	42,818
10 20 30 40 50 60 MSVSGLKAEL KFLASIFDKN HERFRIVSWK LDELHCQFLV PQQGSPHSLP PPLTLHCNIT 70 80 90 100 110 120 ESYPSSSPIW FVDSEDPNLT SVLERLEDTK NNNLLRQQLK WLICELCSLY NLPKHLDVEM 130 140 150 160 170 180 LDQPLPTGQN GTTEEVTSEE EEEEEEMAED IEDLDHYEMK EEEPISGKKS EDEGIEKENL 190 200 210 220 230 240 AILEKIRKTQ RQDHLNGAVS GSVQASDRLM KELRDIYRSQ SYKTGIYSVE LINDSLYDWH 250 260 270 280 290 300 VKLQKVDPDS PLHSDLQILK EKEGIEYILL NFSFKDNFPF DPPFVRVVLP VLSGGYVLGG 310 320 330 340 350 360 GALCMELLTK QGWSSAYSIE SVIMQINATL VKGKARVQFG ANKNQYNLAR AQQSYNSIVQ 370 IHEKNGWYTP PKEDG « Hide
Isoform 2. Checksum: 2C8943AFD4F6EE8A Show »	FASTA	314	36,135
10 20 30 40 50 60 MSVSGLKAEL KFLASIFDKN HERFRIVSWK LDELHCQFLV PQQGSPHSLP PPLTLHCNIT 70 80 90 100 110 120 ESYPSSSPIW FVDSEDPNLT SVLERLEDTK NNNLLRQQLK WLICELCSLY NLPKHLDVEM 130 140 150 160 170 180 LDQPLPTGQN GTTEEVTSEE EEEEEEMAED IEDLDHYEMK EEEPISGKKS EDEGIEKENL 190 200 210 220 230 240 AILEKIRKTQ RQDHLNGAVS GSVQASDRLM KELRDIYRSQ SYKTGIYSVE LINDSLYDWH 250 260 270 280 290 300 VKLQKVDPDS PLHSDLQILK EKEGIEYILL NFSFKDNFPF DPPFVRVVLP VLSGGLVHPS 310 KGRWLNMLTV VCLD « Hide

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	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Melanoma.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 116-375 (ISOFORM 2). Tissue: Ovary, Placenta and Uterus.
[3]	"Analysis of a novel human gene, LOC92912, over-expressed in hypopharyngeal tumours." Seghatoleslam A., Zambrano A., Millon R., Ganguli G., Argentini M., Cromer A., Abecassis J., Wasylyk B. Biochem. Biophys. Res. Commun. 339:422-429(2006) [PubMed: 16300736] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-369, MASS SPECTROMETRY.
[5]	"Structure of human ubiquitin-conjugating enzyme (UBCI) involved in embryo attachment and implantation." Structural genomics consortium (SGC) Submitted (JAN-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 197-363.
+	Additional computationally mapped references.

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AL832429 mRNA. Translation: CAH10654.1.
BC006827 mRNA. Translation: AAH06827.1.
BC017708 mRNA. Translation: AAH17708.1.
BC034342 mRNA. Translation: AAH34342.1. Different initiation.

IPI

IPI00103165.
IPI00386815.

RefSeq

NP_775740.1.

UniGene

Hs.714732

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZUO	X-ray	1.80	A/B	197-363	[»]

ModBase

Search...

PhosphoSite

Q8WVN8.

PRIDE

Q8WVN8.

Ensembl

ENSG00000140367. Homo sapiens. [Contig view]

GeneID

92912.

KEGG

hsa:92912.

GeneCards

GC15P073923.

H-InvDB

HIX0012448.
HIX0038395.

HGNC

HGNC:19248. UBE2Q2.

MIM

612501. gene.

PharmGKB

PA142670652.

GenAtlas

Search...

HOGENOM

Q8WVN8.

HOVERGEN

Q8WVN8.

OMA

Q8WVN8. YKAGIYS.

BRENDA

6.3.2.19. 247.

ArrayExpress

Q8WVN8.

Bgee

Q8WVN8.

CleanEx

HS_UBE2Q2.

GermOnline

ENSG00000140367. Homo sapiens.

InterPro

IPR006575. RWD.
IPR016135. UBQ-conjugat/RWD-like.
IPR000608. UBQ-conjugat_E2.
[Graphical view]

Gene3D

G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.

Pfam

PF00179. UQ_con. 1 hit.
[Graphical view]

ProDom

PD000461. UBQ_conjugat. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00591. RWD. 1 hit.
SM00212. UBCc. 1 hit.
[Graphical view]

PROSITE

PS00183. UBIQUITIN_CONJUGAT_1. False negative.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

NextBio

77912.

SOURCE

Search...

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This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8WVN8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8WVN8-2)

The sequence of this isoform differs from the canonical sequence as follows:
296-314: YVLGGGALCMELLTKQGWS → LVHPSKGRWLNMLTVVCLD
315-375: Missing.

Entry name

UB2Q2_HUMAN

Accession

Primary (citable) accession number: Q8WVN8
Secondary accession number(s): Q8N4G6, Q96J08

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	March 1, 2002
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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