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Protein

Ubiquitin-conjugating enzyme E2 Q2

Gene

UBE2Q2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei304Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • protein K48-linked ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS06705-MONOMER.
BRENDAi2.3.2.B6. 2681.
ReactomeiR-HSA-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ8WVN8.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 Q2 (EC:2.3.2.231 Publication)
Alternative name(s):
E2 ubiquitin-conjugating enzyme Q2
Ubiquitin carrier protein Q2
Ubiquitin-protein ligase Q2
Gene namesi
Name:UBE2Q2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:19248. UBE2Q2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi92912.
OpenTargetsiENSG00000140367.
PharmGKBiPA142670652.

Polymorphism and mutation databases

BioMutaiUBE2Q2.
DMDMi74751557.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002238791 – 375Ubiquitin-conjugating enzyme E2 Q2Add BLAST375

Proteomic databases

MaxQBiQ8WVN8.
PaxDbiQ8WVN8.
PeptideAtlasiQ8WVN8.
PRIDEiQ8WVN8.

PTM databases

iPTMnetiQ8WVN8.
PhosphoSitePlusiQ8WVN8.

Expressioni

Tissue specificityi

Detected in hypopharyngeal head and neck squamous cell carcinoma, in tumor masses and invasive epithelium.1 Publication

Gene expression databases

BgeeiENSG00000140367.
CleanExiHS_UBE2Q2.
ExpressionAtlasiQ8WVN8. baseline and differential.
GenevisibleiQ8WVN8. HS.

Interactioni

Protein-protein interaction databases

BioGridi124986. 17 interactors.
IntActiQ8WVN8. 8 interactors.
STRINGi9606.ENSP00000267938.

Structurei

Secondary structure

1375
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi203 – 217Combined sources15
Helixi220 – 223Combined sources4
Beta strandi226 – 232Combined sources7
Beta strandi235 – 243Combined sources9
Helixi251 – 263Combined sources13
Beta strandi267 – 273Combined sources7
Turni276 – 279Combined sources4
Beta strandi284 – 290Combined sources7
Beta strandi292 – 294Combined sources3
Helixi299 – 301Combined sources3
Helixi306 – 308Combined sources3
Turni310 – 312Combined sources3
Helixi319 – 332Combined sources14
Helixi348 – 361Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZUOX-ray1.80A/B197-363[»]
ProteinModelPortaliQ8WVN8.
SMRiQ8WVN8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8WVN8.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi134 – 178Glu-richAdd BLAST45

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0897. Eukaryota.
ENOG410XRET. LUCA.
GeneTreeiENSGT00390000018573.
HOGENOMiHOG000007757.
HOVERGENiHBG061368.
InParanoidiQ8WVN8.
KOiK10582.
OMAiSPLRCDQ.
OrthoDBiEOG091G0LGG.
PhylomeDBiQ8WVN8.
TreeFamiTF313338.

Family and domain databases

Gene3Di3.10.110.10. 2 hits.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 2 hits.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WVN8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVSGLKAEL KFLASIFDKN HERFRIVSWK LDELHCQFLV PQQGSPHSLP
60 70 80 90 100
PPLTLHCNIT ESYPSSSPIW FVDSEDPNLT SVLERLEDTK NNNLLRQQLK
110 120 130 140 150
WLICELCSLY NLPKHLDVEM LDQPLPTGQN GTTEEVTSEE EEEEEEMAED
160 170 180 190 200
IEDLDHYEMK EEEPISGKKS EDEGIEKENL AILEKIRKTQ RQDHLNGAVS
210 220 230 240 250
GSVQASDRLM KELRDIYRSQ SYKTGIYSVE LINDSLYDWH VKLQKVDPDS
260 270 280 290 300
PLHSDLQILK EKEGIEYILL NFSFKDNFPF DPPFVRVVLP VLSGGYVLGG
310 320 330 340 350
GALCMELLTK QGWSSAYSIE SVIMQINATL VKGKARVQFG ANKNQYNLAR
360 370
AQQSYNSIVQ IHEKNGWYTP PKEDG
Length:375
Mass (Da):42,818
Last modified:March 1, 2002 - v1
Checksum:i7DE07315E89178A3
GO
Isoform 2 (identifier: Q8WVN8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     296-314: YVLGGGALCMELLTKQGWS → LVHPSKGRWLNMLTVVCLD
     315-375: Missing.

Show »
Length:314
Mass (Da):36,135
Checksum:i2C8943AFD4F6EE8A
GO
Isoform 3 (identifier: Q8WVN8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-60: MSVSGLKAEL...PPLTLHCNIT → MRMDSLTEEK...ERSIVPSLRQ

Note: No experimental confirmation available.
Show »
Length:359
Mass (Da):40,956
Checksum:i6C596548409AEFEB
GO
Isoform 4 (identifier: Q8WVN8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     95-129: Missing.

Note: No experimental confirmation available.
Show »
Length:340
Mass (Da):38,699
Checksum:i580712410C256289
GO

Sequence cautioni

The sequence AAH34342 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti161E → K in BAH12288 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0448171 – 60MSVSG…HCNIT → MRMDSLTEEKLECRLWCCLS DPSPPGLAARCCVLERSIVP SLRQ in isoform 3. 1 PublicationAdd BLAST60
Alternative sequenceiVSP_05568195 – 129Missing in isoform 4. 1 PublicationAdd BLAST35
Alternative sequenceiVSP_017298296 – 314YVLGG…KQGWS → LVHPSKGRWLNMLTVVCLD in isoform 2. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_017299315 – 375Missing in isoform 2. 1 PublicationAdd BLAST61

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000617 mRNA. No translation available.
AK296234 mRNA. Translation: BAH12288.1.
AL832429 mRNA. Translation: CAH10654.1.
AC019294 Genomic DNA. No translation available.
AC027104 Genomic DNA. No translation available.
BC006827 mRNA. Translation: AAH06827.1.
BC017708 mRNA. Translation: AAH17708.1.
BC034342 mRNA. Translation: AAH34342.1. Different initiation.
CCDSiCCDS10286.1. [Q8WVN8-1]
CCDS45309.1. [Q8WVN8-3]
CCDS66839.1. [Q8WVN8-4]
RefSeqiNP_001138807.1. NM_001145335.1. [Q8WVN8-3]
NP_001271311.1. NM_001284382.1. [Q8WVN8-4]
NP_775740.1. NM_173469.3. [Q8WVN8-1]
UniGeneiHs.23033.

Genome annotation databases

EnsembliENST00000267938; ENSP00000267938; ENSG00000140367. [Q8WVN8-1]
ENST00000561851; ENSP00000456229; ENSG00000140367. [Q8WVN8-3]
ENST00000569423; ENSP00000456324; ENSG00000140367. [Q8WVN8-4]
GeneIDi92912.
KEGGihsa:92912.
UCSCiuc002bbg.4. human. [Q8WVN8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000617 mRNA. No translation available.
AK296234 mRNA. Translation: BAH12288.1.
AL832429 mRNA. Translation: CAH10654.1.
AC019294 Genomic DNA. No translation available.
AC027104 Genomic DNA. No translation available.
BC006827 mRNA. Translation: AAH06827.1.
BC017708 mRNA. Translation: AAH17708.1.
BC034342 mRNA. Translation: AAH34342.1. Different initiation.
CCDSiCCDS10286.1. [Q8WVN8-1]
CCDS45309.1. [Q8WVN8-3]
CCDS66839.1. [Q8WVN8-4]
RefSeqiNP_001138807.1. NM_001145335.1. [Q8WVN8-3]
NP_001271311.1. NM_001284382.1. [Q8WVN8-4]
NP_775740.1. NM_173469.3. [Q8WVN8-1]
UniGeneiHs.23033.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZUOX-ray1.80A/B197-363[»]
ProteinModelPortaliQ8WVN8.
SMRiQ8WVN8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124986. 17 interactors.
IntActiQ8WVN8. 8 interactors.
STRINGi9606.ENSP00000267938.

PTM databases

iPTMnetiQ8WVN8.
PhosphoSitePlusiQ8WVN8.

Polymorphism and mutation databases

BioMutaiUBE2Q2.
DMDMi74751557.

Proteomic databases

MaxQBiQ8WVN8.
PaxDbiQ8WVN8.
PeptideAtlasiQ8WVN8.
PRIDEiQ8WVN8.

Protocols and materials databases

DNASUi92912.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000267938; ENSP00000267938; ENSG00000140367. [Q8WVN8-1]
ENST00000561851; ENSP00000456229; ENSG00000140367. [Q8WVN8-3]
ENST00000569423; ENSP00000456324; ENSG00000140367. [Q8WVN8-4]
GeneIDi92912.
KEGGihsa:92912.
UCSCiuc002bbg.4. human. [Q8WVN8-1]

Organism-specific databases

CTDi92912.
DisGeNETi92912.
GeneCardsiUBE2Q2.
H-InvDBHIX0172650.
HGNCiHGNC:19248. UBE2Q2.
MIMi612501. gene.
neXtProtiNX_Q8WVN8.
OpenTargetsiENSG00000140367.
PharmGKBiPA142670652.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0897. Eukaryota.
ENOG410XRET. LUCA.
GeneTreeiENSGT00390000018573.
HOGENOMiHOG000007757.
HOVERGENiHBG061368.
InParanoidiQ8WVN8.
KOiK10582.
OMAiSPLRCDQ.
OrthoDBiEOG091G0LGG.
PhylomeDBiQ8WVN8.
TreeFamiTF313338.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:HS06705-MONOMER.
BRENDAi2.3.2.B6. 2681.
ReactomeiR-HSA-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ8WVN8.

Miscellaneous databases

ChiTaRSiUBE2Q2. human.
EvolutionaryTraceiQ8WVN8.
GenomeRNAii92912.
PROiQ8WVN8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000140367.
CleanExiHS_UBE2Q2.
ExpressionAtlasiQ8WVN8. baseline and differential.
GenevisibleiQ8WVN8. HS.

Family and domain databases

Gene3Di3.10.110.10. 2 hits.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 2 hits.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUB2Q2_HUMAN
AccessioniPrimary (citable) accession number: Q8WVN8
Secondary accession number(s): B7Z3Q2
, H3BRG2, Q8N4G6, Q96J08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.