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Q8WVN8

- UB2Q2_HUMAN

UniProt

Q8WVN8 - UB2Q2_HUMAN

Protein

Ubiquitin-conjugating enzyme E2 Q2

Gene

UBE2Q2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination.1 Publication

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei304 – 3041Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. protein K48-linked ubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
    SignaLinkiQ8WVN8.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 Q2 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein Q2
    Ubiquitin-protein ligase Q2
    Gene namesi
    Name:UBE2Q2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:19248. UBE2Q2.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142670652.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 375375Ubiquitin-conjugating enzyme E2 Q2PRO_0000223879Add
    BLAST

    Proteomic databases

    MaxQBiQ8WVN8.
    PaxDbiQ8WVN8.
    PRIDEiQ8WVN8.

    PTM databases

    PhosphoSiteiQ8WVN8.

    Expressioni

    Tissue specificityi

    Detected in hypopharyngeal head and neck squamous cell carcinoma, in tumor masses and invasive epithelium.1 Publication

    Gene expression databases

    ArrayExpressiQ8WVN8.
    BgeeiQ8WVN8.
    CleanExiHS_UBE2Q2.
    GenevestigatoriQ8WVN8.

    Interactioni

    Protein-protein interaction databases

    BioGridi124986. 15 interactions.
    IntActiQ8WVN8. 8 interactions.
    STRINGi9606.ENSP00000267938.

    Structurei

    Secondary structure

    1
    375
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi203 – 21715
    Helixi220 – 2234
    Beta strandi226 – 2327
    Beta strandi235 – 2439
    Helixi251 – 26313
    Beta strandi267 – 2737
    Turni276 – 2794
    Beta strandi284 – 2907
    Beta strandi292 – 2943
    Helixi299 – 3013
    Helixi306 – 3083
    Turni310 – 3123
    Helixi319 – 33214
    Helixi348 – 36114

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ZUOX-ray1.80A/B197-363[»]
    ProteinModelPortaliQ8WVN8.
    SMRiQ8WVN8. Positions 201-362.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8WVN8.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi134 – 17845Glu-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG320521.
    HOGENOMiHOG000007757.
    HOVERGENiHBG061368.
    InParanoidiQ8WVN8.
    KOiK10582.
    OMAiSYKAGIY.
    OrthoDBiEOG76DTSS.
    PhylomeDBiQ8WVN8.
    TreeFamiTF313338.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR006575. RWD-domain.
    IPR000608. UBQ-conjugat_E2.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF05773. RWD. 1 hit.
    PF00179. UQ_con. 1 hit.
    [Graphical view]
    SMARTiSM00591. RWD. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 2 hits.
    PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8WVN8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSVSGLKAEL KFLASIFDKN HERFRIVSWK LDELHCQFLV PQQGSPHSLP    50
    PPLTLHCNIT ESYPSSSPIW FVDSEDPNLT SVLERLEDTK NNNLLRQQLK 100
    WLICELCSLY NLPKHLDVEM LDQPLPTGQN GTTEEVTSEE EEEEEEMAED 150
    IEDLDHYEMK EEEPISGKKS EDEGIEKENL AILEKIRKTQ RQDHLNGAVS 200
    GSVQASDRLM KELRDIYRSQ SYKTGIYSVE LINDSLYDWH VKLQKVDPDS 250
    PLHSDLQILK EKEGIEYILL NFSFKDNFPF DPPFVRVVLP VLSGGYVLGG 300
    GALCMELLTK QGWSSAYSIE SVIMQINATL VKGKARVQFG ANKNQYNLAR 350
    AQQSYNSIVQ IHEKNGWYTP PKEDG 375
    Length:375
    Mass (Da):42,818
    Last modified:March 1, 2002 - v1
    Checksum:i7DE07315E89178A3
    GO
    Isoform 2 (identifier: Q8WVN8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         296-314: YVLGGGALCMELLTKQGWS → LVHPSKGRWLNMLTVVCLD
         315-375: Missing.

    Show »
    Length:314
    Mass (Da):36,135
    Checksum:i2C8943AFD4F6EE8A
    GO
    Isoform 3 (identifier: Q8WVN8-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-60: MSVSGLKAEL...PPLTLHCNIT → MRMDSLTEEK...ERSIVPSLRQ

    Note: No experimental confirmation available.

    Show »
    Length:359
    Mass (Da):40,956
    Checksum:i6C596548409AEFEB
    GO
    Isoform 4 (identifier: Q8WVN8-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         95-129: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:340
    Mass (Da):38,699
    Checksum:i580712410C256289
    GO

    Sequence cautioni

    The sequence AAH34342.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti161 – 1611E → K in BAH12288. (PubMed:14702039)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 6060MSVSG…HCNIT → MRMDSLTEEKLECRLWCCLS DPSPPGLAARCCVLERSIVP SLRQ in isoform 3. 1 PublicationVSP_044817Add
    BLAST
    Alternative sequencei95 – 12935Missing in isoform 4. 1 PublicationVSP_055681Add
    BLAST
    Alternative sequencei296 – 31419YVLGG…KQGWS → LVHPSKGRWLNMLTVVCLD in isoform 2. 1 PublicationVSP_017298Add
    BLAST
    Alternative sequencei315 – 37561Missing in isoform 2. 1 PublicationVSP_017299Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK000617 mRNA. No translation available.
    AK296234 mRNA. Translation: BAH12288.1.
    AL832429 mRNA. Translation: CAH10654.1.
    AC019294 Genomic DNA. No translation available.
    AC027104 Genomic DNA. No translation available.
    BC006827 mRNA. Translation: AAH06827.1.
    BC017708 mRNA. Translation: AAH17708.1.
    BC034342 mRNA. Translation: AAH34342.1. Different initiation.
    CCDSiCCDS10286.1. [Q8WVN8-1]
    CCDS45309.1. [Q8WVN8-3]
    RefSeqiNP_001138807.1. NM_001145335.1. [Q8WVN8-3]
    NP_001271311.1. NM_001284382.1.
    NP_775740.1. NM_173469.3. [Q8WVN8-1]
    UniGeneiHs.23033.

    Genome annotation databases

    EnsembliENST00000267938; ENSP00000267938; ENSG00000140367. [Q8WVN8-1]
    ENST00000561851; ENSP00000456229; ENSG00000140367. [Q8WVN8-3]
    ENST00000569423; ENSP00000456324; ENSG00000140367. [Q8WVN8-4]
    GeneIDi92912.
    KEGGihsa:92912.
    UCSCiuc002bbg.2. human. [Q8WVN8-1]

    Polymorphism databases

    DMDMi74751557.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK000617 mRNA. No translation available.
    AK296234 mRNA. Translation: BAH12288.1 .
    AL832429 mRNA. Translation: CAH10654.1 .
    AC019294 Genomic DNA. No translation available.
    AC027104 Genomic DNA. No translation available.
    BC006827 mRNA. Translation: AAH06827.1 .
    BC017708 mRNA. Translation: AAH17708.1 .
    BC034342 mRNA. Translation: AAH34342.1 . Different initiation.
    CCDSi CCDS10286.1. [Q8WVN8-1 ]
    CCDS45309.1. [Q8WVN8-3 ]
    RefSeqi NP_001138807.1. NM_001145335.1. [Q8WVN8-3 ]
    NP_001271311.1. NM_001284382.1.
    NP_775740.1. NM_173469.3. [Q8WVN8-1 ]
    UniGenei Hs.23033.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ZUO X-ray 1.80 A/B 197-363 [» ]
    ProteinModelPortali Q8WVN8.
    SMRi Q8WVN8. Positions 201-362.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 124986. 15 interactions.
    IntActi Q8WVN8. 8 interactions.
    STRINGi 9606.ENSP00000267938.

    PTM databases

    PhosphoSitei Q8WVN8.

    Polymorphism databases

    DMDMi 74751557.

    Proteomic databases

    MaxQBi Q8WVN8.
    PaxDbi Q8WVN8.
    PRIDEi Q8WVN8.

    Protocols and materials databases

    DNASUi 92912.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000267938 ; ENSP00000267938 ; ENSG00000140367 . [Q8WVN8-1 ]
    ENST00000561851 ; ENSP00000456229 ; ENSG00000140367 . [Q8WVN8-3 ]
    ENST00000569423 ; ENSP00000456324 ; ENSG00000140367 . [Q8WVN8-4 ]
    GeneIDi 92912.
    KEGGi hsa:92912.
    UCSCi uc002bbg.2. human. [Q8WVN8-1 ]

    Organism-specific databases

    CTDi 92912.
    GeneCardsi GC15P076135.
    H-InvDB HIX0172650.
    HGNCi HGNC:19248. UBE2Q2.
    MIMi 612501. gene.
    neXtProti NX_Q8WVN8.
    PharmGKBi PA142670652.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG320521.
    HOGENOMi HOG000007757.
    HOVERGENi HBG061368.
    InParanoidi Q8WVN8.
    KOi K10582.
    OMAi SYKAGIY.
    OrthoDBi EOG76DTSS.
    PhylomeDBi Q8WVN8.
    TreeFami TF313338.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
    SignaLinki Q8WVN8.

    Miscellaneous databases

    ChiTaRSi UBE2Q2. human.
    EvolutionaryTracei Q8WVN8.
    GenomeRNAii 92912.
    NextBioi 77912.
    PROi Q8WVN8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8WVN8.
    Bgeei Q8WVN8.
    CleanExi HS_UBE2Q2.
    Genevestigatori Q8WVN8.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR006575. RWD-domain.
    IPR000608. UBQ-conjugat_E2.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF05773. RWD. 1 hit.
    PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SMARTi SM00591. RWD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 2 hits.
    PROSITEi PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
      Tissue: Thalamus.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Melanoma.
    3. "Analysis of the DNA sequence and duplication history of human chromosome 15."
      Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
      , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
      Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 116-375 (ISOFORM 2).
      Tissue: Ovary, Placenta and Uterus.
    5. "Analysis of a novel human gene, LOC92912, over-expressed in hypopharyngeal tumours."
      Seghatoleslam A., Zambrano A., Millon R., Ganguli G., Argentini M., Cromer A., Abecassis J., Wasylyk B.
      Biochem. Biophys. Res. Commun. 339:422-429(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    6. "The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines."
      David Y., Ziv T., Admon A., Navon A.
      J. Biol. Chem. 285:8595-8604(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Structure of human ubiquitin-conjugating enzyme (UBCI) involved in embryo attachment and implantation."
      Structural genomics consortium (SGC)
      Submitted (JAN-2006) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 197-363.

    Entry informationi

    Entry nameiUB2Q2_HUMAN
    AccessioniPrimary (citable) accession number: Q8WVN8
    Secondary accession number(s): B7Z3Q2
    , H3BRG2, Q8N4G6, Q96J08
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2006
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3