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Q8WVN8

- UB2Q2_HUMAN

UniProt

Q8WVN8 - UB2Q2_HUMAN

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Protein

Ubiquitin-conjugating enzyme E2 Q2

Gene

UBE2Q2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei304 – 3041Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. protein K48-linked ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ8WVN8.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 Q2 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein Q2
Ubiquitin-protein ligase Q2
Gene namesi
Name:UBE2Q2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 15

Organism-specific databases

HGNCiHGNC:19248. UBE2Q2.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142670652.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Ubiquitin-conjugating enzyme E2 Q2PRO_0000223879Add
BLAST

Proteomic databases

MaxQBiQ8WVN8.
PaxDbiQ8WVN8.
PRIDEiQ8WVN8.

PTM databases

PhosphoSiteiQ8WVN8.

Expressioni

Tissue specificityi

Detected in hypopharyngeal head and neck squamous cell carcinoma, in tumor masses and invasive epithelium.1 Publication

Gene expression databases

BgeeiQ8WVN8.
CleanExiHS_UBE2Q2.
ExpressionAtlasiQ8WVN8. baseline and differential.
GenevestigatoriQ8WVN8.

Interactioni

Protein-protein interaction databases

BioGridi124986. 16 interactions.
IntActiQ8WVN8. 8 interactions.
STRINGi9606.ENSP00000267938.

Structurei

Secondary structure

1
375
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi203 – 21715Combined sources
Helixi220 – 2234Combined sources
Beta strandi226 – 2327Combined sources
Beta strandi235 – 2439Combined sources
Helixi251 – 26313Combined sources
Beta strandi267 – 2737Combined sources
Turni276 – 2794Combined sources
Beta strandi284 – 2907Combined sources
Beta strandi292 – 2943Combined sources
Helixi299 – 3013Combined sources
Helixi306 – 3083Combined sources
Turni310 – 3123Combined sources
Helixi319 – 33214Combined sources
Helixi348 – 36114Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZUOX-ray1.80A/B197-363[»]
ProteinModelPortaliQ8WVN8.
SMRiQ8WVN8. Positions 201-362.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8WVN8.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi134 – 17845Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG320521.
GeneTreeiENSGT00390000018573.
HOGENOMiHOG000007757.
HOVERGENiHBG061368.
InParanoidiQ8WVN8.
KOiK10582.
OMAiSYKAGIY.
OrthoDBiEOG76DTSS.
PhylomeDBiQ8WVN8.
TreeFamiTF313338.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR006575. RWD-domain.
IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF05773. RWD. 1 hit.
PF00179. UQ_con. 1 hit.
[Graphical view]
SMARTiSM00591. RWD. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 2 hits.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8WVN8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVSGLKAEL KFLASIFDKN HERFRIVSWK LDELHCQFLV PQQGSPHSLP
60 70 80 90 100
PPLTLHCNIT ESYPSSSPIW FVDSEDPNLT SVLERLEDTK NNNLLRQQLK
110 120 130 140 150
WLICELCSLY NLPKHLDVEM LDQPLPTGQN GTTEEVTSEE EEEEEEMAED
160 170 180 190 200
IEDLDHYEMK EEEPISGKKS EDEGIEKENL AILEKIRKTQ RQDHLNGAVS
210 220 230 240 250
GSVQASDRLM KELRDIYRSQ SYKTGIYSVE LINDSLYDWH VKLQKVDPDS
260 270 280 290 300
PLHSDLQILK EKEGIEYILL NFSFKDNFPF DPPFVRVVLP VLSGGYVLGG
310 320 330 340 350
GALCMELLTK QGWSSAYSIE SVIMQINATL VKGKARVQFG ANKNQYNLAR
360 370
AQQSYNSIVQ IHEKNGWYTP PKEDG
Length:375
Mass (Da):42,818
Last modified:March 1, 2002 - v1
Checksum:i7DE07315E89178A3
GO
Isoform 2 (identifier: Q8WVN8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     296-314: YVLGGGALCMELLTKQGWS → LVHPSKGRWLNMLTVVCLD
     315-375: Missing.

Show »
Length:314
Mass (Da):36,135
Checksum:i2C8943AFD4F6EE8A
GO
Isoform 3 (identifier: Q8WVN8-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-60: MSVSGLKAEL...PPLTLHCNIT → MRMDSLTEEK...ERSIVPSLRQ

Note: No experimental confirmation available.

Show »
Length:359
Mass (Da):40,956
Checksum:i6C596548409AEFEB
GO
Isoform 4 (identifier: Q8WVN8-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     95-129: Missing.

Note: No experimental confirmation available.

Show »
Length:340
Mass (Da):38,699
Checksum:i580712410C256289
GO

Sequence cautioni

The sequence AAH34342.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti161 – 1611E → K in BAH12288. (PubMed:14702039)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6060MSVSG…HCNIT → MRMDSLTEEKLECRLWCCLS DPSPPGLAARCCVLERSIVP SLRQ in isoform 3. 1 PublicationVSP_044817Add
BLAST
Alternative sequencei95 – 12935Missing in isoform 4. 1 PublicationVSP_055681Add
BLAST
Alternative sequencei296 – 31419YVLGG…KQGWS → LVHPSKGRWLNMLTVVCLD in isoform 2. 1 PublicationVSP_017298Add
BLAST
Alternative sequencei315 – 37561Missing in isoform 2. 1 PublicationVSP_017299Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000617 mRNA. No translation available.
AK296234 mRNA. Translation: BAH12288.1.
AL832429 mRNA. Translation: CAH10654.1.
AC019294 Genomic DNA. No translation available.
AC027104 Genomic DNA. No translation available.
BC006827 mRNA. Translation: AAH06827.1.
BC017708 mRNA. Translation: AAH17708.1.
BC034342 mRNA. Translation: AAH34342.1. Different initiation.
CCDSiCCDS10286.1. [Q8WVN8-1]
CCDS45309.1. [Q8WVN8-3]
CCDS66839.1. [Q8WVN8-4]
RefSeqiNP_001138807.1. NM_001145335.1. [Q8WVN8-3]
NP_001271311.1. NM_001284382.1. [Q8WVN8-4]
NP_775740.1. NM_173469.3. [Q8WVN8-1]
UniGeneiHs.23033.

Genome annotation databases

EnsembliENST00000267938; ENSP00000267938; ENSG00000140367. [Q8WVN8-1]
ENST00000561851; ENSP00000456229; ENSG00000140367. [Q8WVN8-3]
ENST00000569423; ENSP00000456324; ENSG00000140367. [Q8WVN8-4]
GeneIDi92912.
KEGGihsa:92912.
UCSCiuc002bbg.2. human. [Q8WVN8-1]

Polymorphism databases

DMDMi74751557.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000617 mRNA. No translation available.
AK296234 mRNA. Translation: BAH12288.1 .
AL832429 mRNA. Translation: CAH10654.1 .
AC019294 Genomic DNA. No translation available.
AC027104 Genomic DNA. No translation available.
BC006827 mRNA. Translation: AAH06827.1 .
BC017708 mRNA. Translation: AAH17708.1 .
BC034342 mRNA. Translation: AAH34342.1 . Different initiation.
CCDSi CCDS10286.1. [Q8WVN8-1 ]
CCDS45309.1. [Q8WVN8-3 ]
CCDS66839.1. [Q8WVN8-4 ]
RefSeqi NP_001138807.1. NM_001145335.1. [Q8WVN8-3 ]
NP_001271311.1. NM_001284382.1. [Q8WVN8-4 ]
NP_775740.1. NM_173469.3. [Q8WVN8-1 ]
UniGenei Hs.23033.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ZUO X-ray 1.80 A/B 197-363 [» ]
ProteinModelPortali Q8WVN8.
SMRi Q8WVN8. Positions 201-362.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 124986. 16 interactions.
IntActi Q8WVN8. 8 interactions.
STRINGi 9606.ENSP00000267938.

PTM databases

PhosphoSitei Q8WVN8.

Polymorphism databases

DMDMi 74751557.

Proteomic databases

MaxQBi Q8WVN8.
PaxDbi Q8WVN8.
PRIDEi Q8WVN8.

Protocols and materials databases

DNASUi 92912.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000267938 ; ENSP00000267938 ; ENSG00000140367 . [Q8WVN8-1 ]
ENST00000561851 ; ENSP00000456229 ; ENSG00000140367 . [Q8WVN8-3 ]
ENST00000569423 ; ENSP00000456324 ; ENSG00000140367 . [Q8WVN8-4 ]
GeneIDi 92912.
KEGGi hsa:92912.
UCSCi uc002bbg.2. human. [Q8WVN8-1 ]

Organism-specific databases

CTDi 92912.
GeneCardsi GC15P076135.
H-InvDB HIX0172650.
HGNCi HGNC:19248. UBE2Q2.
MIMi 612501. gene.
neXtProti NX_Q8WVN8.
PharmGKBi PA142670652.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG320521.
GeneTreei ENSGT00390000018573.
HOGENOMi HOG000007757.
HOVERGENi HBG061368.
InParanoidi Q8WVN8.
KOi K10582.
OMAi SYKAGIY.
OrthoDBi EOG76DTSS.
PhylomeDBi Q8WVN8.
TreeFami TF313338.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinki Q8WVN8.

Miscellaneous databases

ChiTaRSi UBE2Q2. human.
EvolutionaryTracei Q8WVN8.
GenomeRNAii 92912.
NextBioi 77912.
PROi Q8WVN8.
SOURCEi Search...

Gene expression databases

Bgeei Q8WVN8.
CleanExi HS_UBE2Q2.
ExpressionAtlasi Q8WVN8. baseline and differential.
Genevestigatori Q8WVN8.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR006575. RWD-domain.
IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF05773. RWD. 1 hit.
PF00179. UQ_con. 1 hit.
[Graphical view ]
SMARTi SM00591. RWD. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 2 hits.
PROSITEi PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
    Tissue: Thalamus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Melanoma.
  3. "Analysis of the DNA sequence and duplication history of human chromosome 15."
    Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
    , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
    Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 116-375 (ISOFORM 2).
    Tissue: Ovary, Placenta and Uterus.
  5. "Analysis of a novel human gene, LOC92912, over-expressed in hypopharyngeal tumours."
    Seghatoleslam A., Zambrano A., Millon R., Ganguli G., Argentini M., Cromer A., Abecassis J., Wasylyk B.
    Biochem. Biophys. Res. Commun. 339:422-429(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines."
    David Y., Ziv T., Admon A., Navon A.
    J. Biol. Chem. 285:8595-8604(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Structure of human ubiquitin-conjugating enzyme (UBCI) involved in embryo attachment and implantation."
    Structural genomics consortium (SGC)
    Submitted (JAN-2006) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 197-363.

Entry informationi

Entry nameiUB2Q2_HUMAN
AccessioniPrimary (citable) accession number: Q8WVN8
Secondary accession number(s): B7Z3Q2
, H3BRG2, Q8N4G6, Q96J08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: March 1, 2002
Last modified: November 26, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3