Q8WVN8 (UB2Q2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 Q2
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein Q2
Ubiquitin-protein ligase Q2

Gene names

Name:

UBE2Q2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Ref.6
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Cytoplasm Ref.5.
Tissue specificity	Detected in hypopharyngeal head and neck squamous cell carcinoma, in tumor masses and invasive epithelium. Ref.5
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.
Sequence caution	The sequence AAH34342.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein K48-linked ubiquitination Inferred from direct assay Ref.6. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from direct assay Ref.6. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8WVN8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8WVN8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     296-314: YVLGGGALCMELLTKQGWS → LVHPSKGRWLNMLTVVCLD
     315-375: Missing.

Isoform 3 (identifier: Q8WVN8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-60: MSVSGLKAEL...PPLTLHCNIT → MRMDSLTEEK...ERSIVPSLRQ

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 375

375

Ubiquitin-conjugating enzyme E2 Q2

PRO_0000223879

Regions

Compositional bias

134 – 178

45

Glu-rich

Sites

Active site

304

1

Glycyl thioester intermediate By similarity

Natural variations

Alternative sequence

1 – 60

60

MSVSG…HCNIT → MRMDSLTEEKLECRLWCCLS DPSPPGLAARCCVLERSIVP SLRQ in isoform 3.

VSP_044817

Alternative sequence

296 – 314

19

YVLGG…KQGWS → LVHPSKGRWLNMLTVVCLD in isoform 2.

VSP_017298

Alternative sequence

315 – 375

61

Missing in isoform 2.

VSP_017299

Experimental info

Sequence conflict

161

1

E → K in BAH12288. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

375

Helix Strand Turn

Details...

Helix

203 – 217

15

Helix

220 – 223

4

Beta strand

226 – 232

7

Beta strand

235 – 243

9

Helix

251 – 263

13

Beta strand

267 – 273

7

Turn

276 – 279

4

Beta strand

284 – 290

7

Beta strand

292 – 294

3

Helix

299 – 301

3

Helix

306 – 308

3

Turn

310 – 312

3

Helix

319 – 332

14

Helix

348 – 361

14

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 7DE07315E89178A3	FASTA	375	42,818
	10 20 30 40 50 60 MSVSGLKAEL KFLASIFDKN HERFRIVSWK LDELHCQFLV PQQGSPHSLP PPLTLHCNIT 70 80 90 100 110 120 ESYPSSSPIW FVDSEDPNLT SVLERLEDTK NNNLLRQQLK WLICELCSLY NLPKHLDVEM 130 140 150 160 170 180 LDQPLPTGQN GTTEEVTSEE EEEEEEMAED IEDLDHYEMK EEEPISGKKS EDEGIEKENL 190 200 210 220 230 240 AILEKIRKTQ RQDHLNGAVS GSVQASDRLM KELRDIYRSQ SYKTGIYSVE LINDSLYDWH 250 260 270 280 290 300 VKLQKVDPDS PLHSDLQILK EKEGIEYILL NFSFKDNFPF DPPFVRVVLP VLSGGYVLGG 310 320 330 340 350 360 GALCMELLTK QGWSSAYSIE SVIMQINATL VKGKARVQFG ANKNQYNLAR AQQSYNSIVQ 370 IHEKNGWYTP PKEDG
	Isoform 2 [UniParc]. Checksum: 2C8943AFD4F6EE8A	FASTA	314	36,135
	10 20 30 40 50 60 MSVSGLKAEL KFLASIFDKN HERFRIVSWK LDELHCQFLV PQQGSPHSLP PPLTLHCNIT 70 80 90 100 110 120 ESYPSSSPIW FVDSEDPNLT SVLERLEDTK NNNLLRQQLK WLICELCSLY NLPKHLDVEM 130 140 150 160 170 180 LDQPLPTGQN GTTEEVTSEE EEEEEEMAED IEDLDHYEMK EEEPISGKKS EDEGIEKENL 190 200 210 220 230 240 AILEKIRKTQ RQDHLNGAVS GSVQASDRLM KELRDIYRSQ SYKTGIYSVE LINDSLYDWH 250 260 270 280 290 300 VKLQKVDPDS PLHSDLQILK EKEGIEYILL NFSFKDNFPF DPPFVRVVLP VLSGGLVHPS 310 KGRWLNMLTV VCLD
	Isoform 3 [UniParc]. Checksum: 6C596548409AEFEB	FASTA	359	40,956
	10 20 30 40 50 60 MRMDSLTEEK LECRLWCCLS DPSPPGLAAR CCVLERSIVP SLRQESYPSS SPIWFVDSED 70 80 90 100 110 120 PNLTSVLERL EDTKNNNLLR QQLKWLICEL CSLYNLPKHL DVEMLDQPLP TGQNGTTEEV 130 140 150 160 170 180 TSEEEEEEEE MAEDIEDLDH YEMKEEEPIS GKKSEDEGIE KENLAILEKI RKTQRQDHLN 190 200 210 220 230 240 GAVSGSVQAS DRLMKELRDI YRSQSYKTGI YSVELINDSL YDWHVKLQKV DPDSPLHSDL 250 260 270 280 290 300 QILKEKEGIE YILLNFSFKD NFPFDPPFVR VVLPVLSGGY VLGGGALCME LLTKQGWSSA 310 320 330 340 350 YSIESVIMQI NATLVKGKAR VQFGANKNQY NLARAQQSYN SIVQIHEKNG WYTPPKEDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Thalamus.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Melanoma.
[3]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 116-375 (ISOFORM 2). Tissue: Ovary, Placenta and Uterus.
[5]	"Analysis of a novel human gene, LOC92912, over-expressed in hypopharyngeal tumours." Seghatoleslam A., Zambrano A., Millon R., Ganguli G., Argentini M., Cromer A., Abecassis J., Wasylyk B. Biochem. Biophys. Res. Commun. 339:422-429(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]	"The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines." David Y., Ziv T., Admon A., Navon A. J. Biol. Chem. 285:8595-8604(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Structure of human ubiquitin-conjugating enzyme (UBCI) involved in embryo attachment and implantation." Structural genomics consortium (SGC) Submitted (JAN-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 197-363.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK296234 mRNA. Translation: BAH12288.1.
AL832429 mRNA. Translation: CAH10654.1.
AC019294 Genomic DNA. No translation available.
AC027104 Genomic DNA. No translation available.
BC006827 mRNA. Translation: AAH06827.1.
BC017708 mRNA. Translation: AAH17708.1.
BC034342 mRNA. Translation: AAH34342.1. Different initiation.

IPI

IPI00103165.
IPI00386815.
IPI00922714.

RefSeq

NP_001138807.1. NM_001145335.1.
NP_775740.1. NM_173469.2.

UniGene

Hs.740413.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZUO	X-ray	1.80	A/B	197-363	[»]

ProteinModelPortal

Q8WVN8.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8WVN8. 8 interactions.

STRING

9606.ENSP00000267938.

PTM databases

PhosphoSite

Q8WVN8.

Polymorphism databases

DMDM

74751557.

Proteomic databases

PaxDb

Q8WVN8.

PRIDE

Q8WVN8.

Protocols and materials databases

DNASU

92912.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000267938; ENSP00000267938; ENSG00000140367.
ENST00000561851; ENSP00000456229; ENSG00000140367.

GeneID

92912.

KEGG

hsa:92912.

UCSC

uc002bbg.2. human.

Organism-specific databases

CTD

92912.

GeneCards

GC15P076135.

H-InvDB

HIX0172650.

HGNC

HGNC:19248. UBE2Q2.

MIM

612501. gene.

neXtProt

NX_Q8WVN8.

PharmGKB

PA142670652.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG320521.

HOGENOM

HOG000007757.

HOVERGEN

HBG061368.

InParanoid

Q8WVN8.

KO

K10582.

OMA

SYKAGIY.

OrthoDB

EOG48D0VK.

PhylomeDB

Q8WVN8.

Enzyme and pathway databases

Reactome

REACT_6900. Immune System.

UniPathway

UPA00143.

Gene expression databases

ArrayExpress

Q8WVN8.

Bgee

Q8WVN8.

CleanEx

HS_UBE2Q2.

Genevestigator

Q8WVN8.

GermOnline

ENSG00000140367. Homo sapiens.

Family and domain databases

Gene3D

3.10.110.10. 1 hit.

InterPro

IPR006575. RWD-domain.
IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]

Pfam

PF05773. RWD. 1 hit.
PF00179. UQ_con. 1 hit.
[Graphical view]

SMART

SM00591. RWD. 1 hit.
[Graphical view]

SUPFAM

SSF54495. UBQ-conjugat/RWD-like. 2 hits.

PROSITE

PS00183. UBIQUITIN_CONJUGAT_1. False negative.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

UBE2Q2. human.

EvolutionaryTrace

Q8WVN8.

GenomeRNAi

92912.

NextBio

77912.

SOURCE

Search...

Entry information

Entry name

UB2Q2_HUMAN

Accession

Primary (citable) accession number: Q8WVN8
Secondary accession number(s): B7Z3Q2 , H3BRG2, Q8N4G6, Q96J08

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.